JP2847879B2 - Ruminant feed additives - Google Patents

Description

Description: FIELD OF THE INVENTION The present invention relates to a feed additive for ruminant animals, and more particularly, to a method for bypassing the rumen of a ruminant with a biologically active substance from the abomasum and beyond. The present invention relates to a feed additive for ruminants, which is coated and protected by dispersing a biologically active substance in a protective matrix containing a fatty acid metal salt as a main component so as to be absorbed by the digestive organs of the animal.

The ruminant feed additive of the present invention can be orally administered to ruminants such as cattle and sheep by mixing and adding to feed such as concentrated feed and pellets, and biological activity of amino acids, proteins, veterinary drugs, etc. It is suitably used as a preparation that allows a ruminant to absorb a substance with high efficiency.

[Conventional technology]

 Antibiotics such as amino acids, proteins and veterinary drugs
Ruminant rumen stomach when directly orally administered to ruminants
Decomposed by microorganisms in the liquid
It is hardly absorbed. Therefore, these
Oral administration of biologically active substances to ruminants for efficient absorption
For the purpose of collecting, biologically active substances
A formulation protected by a substance that is stable against rumen fluid
Various proposals have been made (JP-A-56-155496,
(See, for example, JP-A-61-151133). The applicant of the present invention also
Biologically active substance protected by adding chitosan to hardened oil etc.
In the digestive tract after the abomasum of the formulation
Good absorbability of biologically active substances with improved disintegration
Formulations have been proposed (JP-A-58-175449, JP-A-59-19).
8946), racquet Marketed under the name
You.

On the other hand, divalent metals such as calcium, magnesium and the like of fatty acids having 14, 16, and / or 18 carbon atoms are used as energy sources that efficiently bypass the rumen of ruminants and are absorbed with high efficiency in the digestive organs after the abomasum. Salt is proposed (USP 4,82
6,694), and calcium salts of mixed fatty acids produced from natural fats and oils (hereinafter referred to as "bypass fats and oils") are commercially available.

In addition, these bypass fats are heated to above their melting point to soften, after adding and mixing the biologically active substance therein, and then cooled, solidified and pulverized. It is disclosed in JP-A-63-313546.

[Problems to be solved by the invention]

In the preparation in which the biologically active substance is coated and protected with the above-mentioned hardened oil or the like, although the ruminal bypass property of the biologically active substance is excellent, the release of the biologically active substance in the digestive organs after the abomasum is performed. Due to its poor sex, it is excreted without being sufficiently absorbed.

In a preparation in which a biologically active substance is coated and protected with a protective substance obtained by adding chitosan to a hardened oil or the like, chitosan acts as a disintegrating agent for the protective substance in the digestive tract of the abomasum and beyond, and the biologically active substance Due to its excellent release in the digestive tract after the four stomachs, the biologically active substance is efficiently absorbed by ruminants. However, since the softening point of the hardened oil is low, improvement in storage stability, particularly, thermal stability at 40 ° C. or higher is required.

On the other hand, bypass oils have a higher melting point than hardened oils and are excellent in thermal stability. Therefore, it is expected that a preparation in which a biologically active substance is coated and protected by using a bypass fat or oil is excellent in the ruminal bypass property of the biologically active substance, the release in the digestive tract after the abomasum and the heat stability. Is done. However, the performance of the preparation, particularly the rapid release in the abomasum, greatly depends on the preparation method and the production conditions of the preparation, and a slight difference in the production conditions causes
No gastric release is achieved.

The present invention provides a ruminant feed additive which is excellent in the heat stability of the preparation and always excellent in the ruminal bypass property of the biologically active substance and in the release property in the fourth and subsequent digestive organs. With that purpose.

[Means for solving the problem]

The present invention provides a feed for ruminants, wherein a biologically active substance is dispersed and protected in a protective matrix containing a fatty acid metal salt having a free metal hydroxide content of 5% by weight or less as a main component. It is an additive.

In the present invention, the biologically active substance is a substance which is provided to an animal and exhibits activities such as promotion of fattening, disease prevention, disease treatment, etc., and particularly when directly orally administered to a ruminant, it is contained in the rumen of a ruminant. It is a substance that is decomposed by microorganisms and hardly exhibits its administration effect.

For example, amino acids such as methionine, lysine and tryptophan; N-acyl amino acids such as N-stearoyl methionine and N-oleyl methionine; calcium salts of N-hydroxymethyl methionine; salts of amino acids such as lysine hydrochloride; Hydroxy homologous compounds of amino acids such as 4-methylmercaptobutyric acid and its calcium salt, fish powder, casein, potato protein, soy protein and other proteins, vitamin A, vitamin A acetate, vitamin A palmitate, vitamin D ₃ Vitamins such as vitamin E, nicotinic acid and nicotinic acid amide, calcium pantetonate, β-carotene, enzymes such as acidic protease, carbohydrates such as glucose, antibiotics such as penicillin and tetracycline, and anthelmintics such as nefphone Veterinary medicine, etc. Is mentioned. These biologically active substances may be used alone or in combination of two or more.

The fatty acid metal salt is, for example, a metal salt of a saturated or unsaturated fatty acid having a straight or branched chain having 8 to 22 carbon atoms, preferably a divalent metal salt, and more preferably a calcium salt. In addition, metal salts of mixed fatty acids of carbon 14, 16 and / or 18 produced from the above-mentioned natural fats and oils can also be used. These fatty acid metal salts usually contain a free metal hydroxide as an impurity derived from the production process, and the fatty acid metal salt used in the present invention has a free metal hydroxide content of 5%. % By weight or less. Within this range, the preparation will have excellent release of the biologically active substance in the digestive tract after the abomasum.

Preferably, a mixed fatty acid metal salt having a melting point of 30 to 50 ° C, more preferably 35 to 45 ° C, is used.

In addition, in order to further improve the rumen bypass property,
C8 to C22 saturated or unsaturated fatty acids having straight or branched chains, higher alcohols, glycerin fatty acid esters, hardened animal and vegetable oils, waxes and the like can be added. Although there is no particular limitation on the ratio of these additions, the melting point as a protective substance is preferably 60 ° C. or higher, particularly preferably 80 ° C. or higher.

The formulation of the present invention further comprises a disintegrating agent which is insoluble in the neutral region and swells, dissolves or decomposes in the acidic region to further improve the dissolution of the biologically active substance in the abomasum. be able to. Examples of such a disintegration imparting agent include chitosan.

Further, for the purpose of adjusting the specific gravity of the preparation, an inorganic filler such as calcium carbonate can be added.

The biologically active substance is prepared in various contents depending on the purpose of administration of the preparation. However, if the amount is too small, the supply amount of the preparation becomes extremely large, which causes inconvenience. On the other hand, if it is too large, the protective matrix does not provide a sufficient effect of covering the biologically active substance, so that the rumen bypass property cannot be achieved.
Therefore, the content of the biologically active substance is preferably 2 to 40% by weight, more preferably 5 to 30% by weight.

The present preparation can be easily produced by mixing the fatty acid metal salt and the biologically active substance, and optionally other additives, heating and softening, kneading, and then molding.

An extrusion granulation method or the like can be adopted as a molding method of the present preparation, and an ordinary extruder for a thermoplastic resin or the like is suitably used as a production apparatus.

〔Example〕

The present invention will be described in more detail with reference to Examples and Comparative Examples.

However, the scope of the present invention is not limited by the following examples.

In the following examples, "parts" and "%"
Unless otherwise specified, it is based on weight.

(1) Preparation of feed additives for ruminant animals Mixed fatty acids having a melting point of 43 ° C (2% myristic acid, 38.5% palmitic acid, 17% stearic acid) at the mixing ratios shown in Table 1
%, Oleic acid 35.4%, linoleic acid 6%, linolenic acid 0.
5%, 0.6% arachidic acid) and a biologically active substance were mixed and melt-kneaded at 130 ° C. After cooling and solidifying to room temperature, the mixture was pulverized and sieved to obtain a granular preparation having a particle size of 1 to 5 mm.

The amount of free calcium hydroxide in the fatty acid calcium salt was measured by extracting free calcium hydroxide into water at room temperature and measuring the amount by free EDTA titration.

(2) Dissolution test of biologically active substance Each 2 g of the sample prepared in the above section (1) was immersed in 200 cc of Tris buffer solution corresponding to ruminal gastric juice of cattle, and was immersed at a temperature of 37 ° C. for 24 hours. After shaking, the mixture was taken out of the Tris buffer, and 0.05 M (= mol · dm ⁻³ ) hydrochloric acid (200 c) corresponding to bovine abomasum gastric juice was used.
c and shaken at 37 ° C. for another 4 hours. Then, the preparation taken out from 0.05M hydrochloric acid was added to cattle small intestine liquid 2
The sample was immersed in 00 cc and shaken at a temperature of 37 ° C. for another 4 hours.

Tris buffer Tris [tris (hydridoxymethyl) aminomethane] 6.
06 g was dissolved in 292 ml of 0.1 M hydrochloric acid and diluted to 1000 ml with water, pH 8.0.Then, methionine and lysine eluted in Tris buffer, 0.05 M hydrochloric acid and the solution corresponding to the small intestine were analyzed by iodometric titration or ninhydrin coloring. Quantified by the method.

 The test results are shown in Table 1.

[Effect of the Invention] As shown in the above Examples, the feed additive for ruminant animals of the present invention, when orally administered to ruminant animals, has the fourth and subsequent digestive organs of biologically active substances contained therein. Is extremely stable and excellent, and the fatty acid metal salt has a high melting point and a high heat resistance as compared with the hardened oil, and thus has extremely excellent storage stability.

The present invention is suitable for allowing a biologically active substance which is liable to be decomposed in the rumen of a ruminant animal when administered orally to bypass the rumen and to be absorbed with high efficiency in the digestive organs after the rumen. Further, the present invention provides a ruminant feed additive having excellent storage stability, particularly excellent heat stability, and is extremely significant in industry, particularly in the livestock industry.

Continuation of the front page (56) References JP-A-56-155496 (JP, A) JP-A-63-313546 (JP, A) JP-A-59-198946 (JP, A) JP-A-58-175449 (JP) , A) JP-A-2-6428 (JP, A) (58) Fields investigated (Int. Cl. ⁶ , DB name) A23K 1/18 A23K 1/16

Claims (3)

(57) [Claims] 1. A ruminant which comprises a biologically active substance dispersed and protected in a protective matrix containing a fatty acid metal salt having a free metal hydroxide content of 5% by weight or less as a main component. Feed additives 2. The feed additive for ruminants according to claim 1, wherein the content of the biologically active substance is 2 to 40% by weight. 3. The metal salt of a fatty acid according to claim 1 or 2, wherein the metal salt of a fatty acid is a metal salt of a mixture of a saturated or unsaturated fatty acid having a straight or branched chain having 8 to 22 carbon atoms. Certain ruminant feed additives