JP3266608B2 - Ruminant feed additives - Google Patents

Description

Description: FIELD OF THE INVENTION The present invention relates to ruminant feed additives, and more particularly to protecting biologically active substances from rumen gastric juice of ruminants. The present invention relates to a preparation in which nuclear granules containing a biologically active substance are coated with a protective film made of a fatty acid salt and a water-insoluble substance compatible with the fatty acid salt so as to be efficiently absorbed in the digestive organs after the four stomachs.

The ruminant feed additive of the present invention can be added to and mixed with a feed and orally administered to ruminants such as cattle and sheep, and efficiently absorbs biologically active substances such as amino acids, proteins, and veterinary drugs. Is used as a pharmaceutical preparation.

[Conventional technology]

When a biologically active substance such as an amino acid, a protein or an animal drug is orally administered to a ruminant, it is decomposed by microorganisms present in the rumen gastric juice of the ruminant and is not absorbed as it is.

 Therefore, these biologically active substances are absorbed efficiently.
For the purpose of collecting, biologically active substances
Protected with a substance that is stable against rumen gastric juice
Rumination that releases biologically active substances in subsequent digestive organs
Various feed additives for foods have been proposed (JP-A-56-1549).
No. 56, JP-A-61-151133, etc.)
Applicants also convert biologically active substances into protective substances such as hydrogenated oils.
Protective Substances Disintegrate in the Digestive Organs after the Abomasum
We propose a formulation protected by adding chitosan for a longer purpose.
(JP-A-58-175449, JP-A-59-198946, etc.
See), Lactet It is marketed under the name of

On the other hand, divalent metals such as calcium, magnesium and the like of fatty acids having 14, 16, and / or 18 carbon atoms are used as an energy source that efficiently bypasses the rumen of ruminants and is absorbed in the digestive organs of the abomasum and beyond with high efficiency. Salt is proposed (USP 4,82
6,694) and calcium salts of mixed fatty acids produced from natural fats and oils (hereinafter referred to as “bypass fats and oils”) are commercially available.

Also known is a method of producing a feed additive for ruminant animals, in which these bypass fats are heated to a temperature above their melting point to soften, and a biologically active substance is added and mixed therein, then cooled, solidified and ground. (See JP-A-63-313546).

[Problems to be solved by the invention]

In the ruminant feed additive in which the biologically active substance is coated and protected with the above-mentioned protective substance such as hydrogenated oil, the ruminal bypass of the biologically active substance and the release in the digestive tract after the abomasum are performed. Extremely excellent in storage stability,
In particular, it is required to improve the thermal stability when stored at 40 ° C. or higher.

On the other hand, bypass fats and oils have a higher melting point than hardened oils and the like, and are excellent in thermal stability. Therefore, a preparation in which a biologically active substance is coated and protected with a bypass fat can be expected to have excellent heat stability, but a method of simply mixing a biologically active substance with the heat-softened bypass fat described in the cited reference. In the preparation manufactured in the above, various factors are superimposed, and the ruminal bypass property of the biologically active substance is inferior. In particular, a preparation containing a high amount of the biologically active substance or a biologically active substance having a high water solubility is used. In the formulation containing, it is difficult to sufficiently cover the biologically active substance with the protective film of the bypass oil or fat, so that the ruminal bypass property of the biologically active substance is hardly obtained.

An object of the present invention is to provide a feed additive for ruminants that uses a bypass fat and oil having a superior ruminal bypass property of a biologically active substance as a protective substance.

[Means for solving the problem]

The present inventors have conducted intensive studies in order to achieve the above object, and as a result, obtained a core granule prepared by using a biologically active substance as a binder with bypass oils and fats, water-insoluble fatty acids, oils and waxes as binders, and further with bypass oils and bypass oils and fats. Coating with a protective substance consisting of a water-insoluble substance that is compatible with
The present inventors have found that the preparation is extremely excellent in gastric release, and completed the present invention.

The present invention relates to a repellent comprising a fatty acid salt which is insoluble in a neutral region and decomposable in an acidic region, and a water-insoluble substance compatible with the fatty acid salt. It is a ruminant feed additive characterized by being coated with a dense protective film having excellent aqueous properties.

In the present invention, the biologically active substance is a substance which shows an activity such as promotion of fattening, disease prevention, disease treatment and the like when given to an animal. It is a substance that is decomposed by microorganisms existing in, and hardly expresses its effect as it is.

For example, amino acids: methionine, lysine, tryptophan and the like; N-acylaminos: calcium salts of N-stearoylmethionine, N-oleylmethionine, N-hydroxymethylmethionine; amino acid salts: lysine hydrochloride and the like; Classes: 2-hydroxy-4-methylmercaptobutyric acid and its calcium salt, etc. Proteins: Fish powder, casein, potato protein, soy protein, etc. Vitamins: Vitamin A, vitamin A acetate, vitamin A palmitate, vitamin
D _3, vitamin E, nicotinic acid and nicotinic acid amide, calcium pantothenate, beta-carotene, enzymes: acid protease or the like, carbohydrates: Fighter sugar and the like, veterinary compounds:
Examples include antibiotics such as penicillin and tetracycline, and anthelmintic drugs such as negphone, and one or more of them are used.

The protective substance used as a binder in the production of nuclear granules is stable in the rumen gastric juice of ruminants in the neutral range of pH 5 to 8, and digested in the abdominal gastric juice or in the abomasum and beyond in the acidic range of pH 3 or less. Any substance that can be decomposed or emulsified by the digestive fluid of the organ may be used.

For example, a metal salt of a saturated or unsaturated fatty acid having a straight or branched chain having 8 to 22 carbon atoms is preferably a divalent metal salt, and more preferably a calcium salt. These can be used alone, but may be a saturated or unsaturated monocarboxylic acid having a straight or branched chain having 14 to 22 carbon atoms, or a saturated or unsaturated monocarboxylic acid having a straight or branched chain having 14 to 22 carbon atoms. Alcohols, glycerin fatty acid esters,
It is also possible to use a mixture with one or more kinds of hardened animal and vegetable oils.

Although the mixing ratio is not particularly specified, the number of carbon atoms is 8 to 22.
It is necessary to use at least 30% by weight or more of a metal salt of a saturated or unsaturated fatty acid having a straight or branched chain, and the melting point of the protective substance is preferably at least 80 ° C. or more. In order to adjust the specific gravity of the preparation, an inorganic filler such as calcium carbonate can be added.

The nuclear granules can be produced by mixing one or more of the above-mentioned biologically active substances with a protective substance and by a usual granulation method. As a production method, a spray granulation method, an extrusion granulation method, a stirring granulation method, or the like can be used.

Fatty acid salts that are insoluble in the neutral region and decomposable in the acidic region used for the protective film are salts of fatty acids that are stable in rumen ruminal fluid of ruminants and are degraded in ruminal gastric fluid like the above-mentioned protective substances. For example, calcium salts of the above-cited fatty acids having 14, 16 and / or 18 carbon atoms, calcium salts of mixed fatty acids produced from natural fats and oils and the like are used.

Preferably the melting point is 30-50 ° C, more preferably 35-45
A calcium salt of a mixed fatty acid at 0 ° C is used.

Water-insoluble substances compatible with the fatty acid salt as one component of the protective film include, for example, fatty acids and alcohols having 8 to 22 carbon atoms having a saturated or unsaturated straight or branched chain, and glycerin monofatty acid esters. Etc. are used. In particular, the above-mentioned fatty acids, alcohols, glycerin monofatty acid esters having a melting point in the range of 55 to 80 ° C, and mixtures thereof are used. Further, in order to further improve the rumen bypass property, hardened animal and vegetable oils, waxes and the like can be added. There is no particular limitation on the proportion of these additions, but when the melting point as a protective substance is measured.
It is preferably 80 ° C. or higher.

The protective film is formed by using a usual coating apparatus, for example, a fluidized bed type or a rolling type apparatus. In order to coat the protective film efficiently, the core granules are preferably as round as possible.

[Action]

The present invention provides, as described above, a nuclear granule containing a biologically active substance, a fatty acid salt and a higher fatty acid, which are insoluble in the neutral region and show degradability in the acidic region, higher alcohols and glycerin monofatty acid esters. A feed additive for ruminant animals, characterized by being coated with a protective film consisting of:

In the present invention, by using a fatty acid salt insoluble in the neutral range and decomposable in the acidic range and a higher fatty acid, a higher alcohol, a fat or the like in the production of the core granules, a more dense core granule is formed than the fatty acid salt alone. As a result, the rumen bypass of ruminants is also improved.

In addition, by using a fatty acid salt that is insoluble in the neutral region and decomposable in the acidic region and a fatty acid compatible with this salt as a protective substance, a higher alcohol, a glycerin fatty acid ester, etc., compared to a film formed only with a fatty acid salt, The membrane quality is improved, the ruminal bypass of biologically active substances is improved, and the abomasum release is also improved. Further, since the melting point is 80 ° C. or higher, storage stability is improved.

In the present invention, the fatty acid salt as a component of the protective film,
Since it is insoluble in the neutral range and degradable in the acidic range, it is extremely stable in the rumen gastric juice of ruminants in the pH range of 5-8 and easily decomposed in the rumen of the ruminant below pH 3 I do. As a result, the biologically active substance contained in the nuclear granules is eluted in the rumen of the ruminant and is efficiently absorbed in the digestive organs thereafter.

In addition, by using a water-insoluble substance that is compatible with fatty acid salts such as fatty acids, alcohols, and glycerin monofatty acid esters as components of the protective film, the film quality becomes denser than a film formed only with fatty acid salts, and biological The elution rate of the active substance in the rumen is reduced and the bypass rate is improved. As a result, the biologically active substance contained in the nuclear granules is eluted in the rumen of the ruminant and is efficiently absorbed in the digestive organs thereafter.

Furthermore, in the present invention, a dense protective film is formed by using a fatty acid salt and a higher fatty acid, a higher alcohol, a fat or the like which are insoluble in a neutral region and decomposable in an acidic region, as a binder at the time of manufacturing the core granules, thereby forming a dense protective film. Thus, the ruminal bypass of the biologically active substance is improved.

〔Example〕

The present invention will be described in more detail with reference to Examples and Comparative Examples.

However, the scope of the present invention is not limited by the following examples.

In the following examples, "parts" and "%"
Unless otherwise specified, it is based on weight.

(1) Preparation of core granules A calcium salt of beef tallow fatty acid having a melting point of 43 ° C. is heated and melt-softened to 130 to 140 ° C., and castor-cured fatty acid and methionine powder are added thereto, and kneaded for about 10 minutes. And cut at a temperature of 90-110 ° C, particle size 1.0-3.4mm
Of nuclear granules A-1 and A-2 were prepared.

Similarly, after mixing calcium salt of tallow fatty acid having a melting point of 43 ° C., stearic acid, lysine hydrochloride powder, and calcium carbonate, kneading, extruding, and cutting, the particle size is 1.0 to 1.2 mm.
Was prepared.

In addition, a calcium salt of tallow fatty acid having a melting point of 43 ° C. is dissolved in a mixed solution of stearic acid and hardened tallow oil heated to 120 ° C., and a slurry obtained by adding lysine hydrochloride powder and calcium carbonate to the mixture is spray-solidified to obtain a particle size. 0.6-0.8 mm nuclear granules A-4 were prepared.

The composition, melting point and particle size of the obtained core granules are shown in Table 1.

(2) Preparation of feed additive for ruminants Nuclear granules prepared in the above item (1): 1 k of A-1 to A-4
g in a Henschel mixer and, while stirring, a castor-hardened fatty acid or a cast liquid containing 120% of a castor-hardened fatty acid and stearic acid in which the same fatty acid calcium salt as used in the above item (1) is dissolved, or A protective film was formed on the surface of the core granules by spraying a melt of 120 ° C. consisting of stearic acid in which the fatty acid calcium salt was dissolved and rapeseed hardened oil to prepare a ruminant feed additive.

Table 2 shows the composition, coating amount, softening point / melting point, and content of the biologically active substance of the coating layer of the obtained feed additive for ruminants.

(3) Dissolution test of biologically active substance Ruminant feed additive prepared in the above item (2) and nuclear granules prepared in the above item (1) for comparison and comparison prepared in item 2 for comparison 2 g of each of the preparations was immersed in 200 cc of Tris buffer corresponding to bovine ruminal gastric juice, kept at 37 ° C. for 24 hours with shaking, removed from the Tris buffer, and added to bovine ruminal gastric juice. Corresponding 0.05M (= mol · dm ^-3 ) hydrochloric acid 200cc
And shaken at 37 ° C. for another 4 hours. Then, the preparation taken out from 0.05M hydrochloric acid was added to bovine small intestine corresponding liquid 200.
The sample was immersed in cc and shaken at a temperature of 37 ° C. for another 4 hours.

Tris buffer Tris [tris (hydroxymethyl) aminomethane] 6.
06 g was dissolved in 292 ml of 0.1 M hydrochloric acid and diluted to 1000 ml with water, pH 8.0 solution.Then, methionine and lysine eluted in Tris buffer, 0.05 M hydrochloric acid and the solution corresponding to the small intestine were analyzed by iodometric titration or ninhydrin coloration. Quantified by the method.

 The test results are shown in Table 2.

(4) Storage stability test Each of the ruminant feed additives prepared in the above section (2)
2 g was stored at 40 ° C. and 60 ° C. for 30 days, and the appearance of the formulation after storage and the dissolution characteristics of the biologically active substance were examined.

The test results are shown in Table 2. After storage for 30 days, if the appearance of the preparation and the dissolution characteristics of the biologically active substance did not change at all from those before the test, it was marked with a circle. The appearance did not change, but the rumen bypass property of the biologically active substance was slightly deteriorated. The product is marked with a triangle, the appearance and dissolution characteristics change greatly, and it is the first biologically active substance.
Those with markedly impaired gastric bypass were indicated by crosses.

As shown in Table 2, in the ruminant feed additive coated with a protective film comprising the fatty acid calcium salt of the present invention and a water-insoluble substance compatible with the fatty acid calcium salt, the ruminant feed additive was not used. The dissolution rate of the biologically active substance is extremely low, and most of the remainder is eluted in the abomasum corresponding liquid and the small intestine corresponding liquid.

On the other hand, in the preparation not covered with the protective film (see Comparative Example), the dissolution rate of the biologically active substance in the rumen-corresponding liquid is large, and the rumen bypass rate of the biologically active substance is 50%. % Or less.

The preparation of the present invention is also excellent in heat resistance, and particularly, a preparation having a melting point of 80 ° C. or more for both the protective substance and the protective film of the core granules exhibits high heat resistance.

〔The invention's effect〕

As shown in the above Examples, the ruminant feed additive of the present invention, when orally administered to a ruminant, has a very excellent ruminal bypass property of a biologically active substance contained therein, Also, since it has high heat resistance, it is extremely excellent in storage stability.

The present invention relates to a ruminant suitable for allowing a biologically active substance, which is easily degraded in the rumen of a ruminant when administered orally, to be efficiently absorbed in the digestive organs of the abomasum and beyond by bypassing the rumen. The present invention provides a feed additive for foods, and its significance in the industry, particularly in the livestock industry, is extremely large.

──────────────────────────────────────────────────続 き Continuation of the front page (72) Inventor Hiroshi Maruyama 12-54, Goi-minamikaigan, Ichihara-shi, Chiba Japan, Functional Products Research Laboratories, Nippon Soda Co., Ltd. (56) References JP-A-60-141242 (JP, A) JP-A-59-198946 (JP, A) JP-A-58-175449 (JP, A) JP-A-56-155496 (JP, A) JP-A-63-313546 (JP, A) (58) Int.Cl. ⁷ , DB name) A23K 1/16 A23K 1/18

Claims (5)

(57) [Claims] 1. A divalent fatty acid having a melting point in the range of 30 to 50.degree. C., which is insoluble in the neutral region and decomposable in the acidic region, in nuclear granules formed from a biologically active substance and a protective substance. A feed additive for ruminants, which is coated with a protective substance containing a metal salt and a water-insoluble substance compatible with the fatty acid salt. 2. The feed additive for ruminants according to claim 1, wherein the biologically active substance is at least one selected from the group consisting of methionine, lysine hydrochloride and vitamins. 3. The ruminant feed additive according to claim 1, wherein the fatty acid is a mixed fatty acid containing an unsaturated fatty acid. 4. The method according to claim 1, wherein the protective substance is
A ruminant comprising a divalent metal salt of a mixed fatty acid having a melting point in the range of 30 to 50 ° C and at least one selected from the group consisting of water-insoluble higher fatty acids, higher alcohols, fatty acid esters of glycerin, oils and waxes. Feed additives for 5. A ruminant according to claim 1, wherein the water-insoluble substance compatible with the fatty acid salt is at least one selected from the group consisting of higher fatty acids, higher alcohols and glycerin monofatty acid esters. feed