JP2847882B2 - Ruminant feed additives - Google Patents
Ruminant feed additivesInfo
- Publication number
- JP2847882B2 JP2847882B2 JP2082552A JP8255290A JP2847882B2 JP 2847882 B2 JP2847882 B2 JP 2847882B2 JP 2082552 A JP2082552 A JP 2082552A JP 8255290 A JP8255290 A JP 8255290A JP 2847882 B2 JP2847882 B2 JP 2847882B2
- Authority
- JP
- Japan
- Prior art keywords
- fatty acid
- biologically active
- preparation
- metal salt
- active substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、反芻動物用飼料添加剤に係り、さらに詳し
くは、反芻動物の第１胃の胃液から生物学的活性物質を
保護し、第４胃以降の消化器官において効率良く吸収さ
せるべく、生物学的活性物質を、脂肪酸金属塩単独又は
脂肪酸金属塩を主成分とする保護マトリックス中に分散
した製剤に関する。Description: FIELD OF THE INVENTION The present invention relates to ruminant feed additives and more particularly to protecting biologically active substances from the rumen gastric juice of ruminants. The present invention relates to a formulation in which a biologically active substance is dispersed in a protective matrix containing a fatty acid metal salt alone or a fatty acid metal salt as a main component, so as to be efficiently absorbed in the digestive organs after the four stomachs.
本発明の反芻動物用飼料添加剤は、飼料に添加混合
し、牛、羊等の反芻動物に経口投与することができ、ア
ミノ酸、タンパク質、動物医薬等の生物学的活性物質を
効率よく吸収させるための製剤として好適に使用され
る。The ruminant feed additive of the present invention can be added to and mixed with feed and orally administered to ruminants such as cows and sheep, and efficiently absorbs biologically active substances such as amino acids, proteins, and veterinary drugs. Is used as a pharmaceutical preparation.
アミノ酸、タンパク質、動物医薬等の生物学的活性物
質を反芻動物に経口投与した場合、反芻動物の第１胃の
胃液に存在する微生物により分解され、そのまま吸収さ
れることはない。When orally administered biologically active substances such as amino acids, proteins and veterinary drugs to ruminants, they are degraded by microorganisms present in the rumen gastric juice of ruminants and are not absorbed as they are.
したがって、これらの生物学的活性物質を効率よく吸
収させることを目的として、生物学的活性物質を油脂等
の第１胃胃液に対して安定な物質で被覆保護し、第４胃
以降の消化器官で生物学的活性物質を放出させる反芻動
物用飼料添加剤が種々提案されており（特開昭56−1549
56号公報、特開昭61−151133号公報等参照）、本発明の
出願人も生物学的活性物質を、硬化油等にその第４胃以
降の消化器官における崩壊性を付与する目的でキトサン
を加えた保護物質で被覆保護した製剤を提案し（特開昭
58−175449号公報、特開昭59−198946号公報等参照）、
ラクテット の名称で上市している。 Therefore, these biologically active substances are absorbed efficiently.
For the purpose of collecting, biologically active substances
Protected with a substance that is stable against ruminal gastric juice,
Ruminant release of biologically active substance in subsequent digestive organs
Various feed additives for foods have been proposed (JP-A-56-1549).
No. 56, JP-A-61-151133, etc.)
Applicant also converts the biologically active substance into a hardened oil
Chitosan for conferring disintegration in the descending digestive tract
Formulation that is coated and protected with a protective substance containing
58-175449, JP-A-59-198946, etc.),
Lactet It is marketed under the name of
一方、反芻動物の第１胃をバイパスし、第４胃以降の
消化器官において高効率で吸収されるエネルギー源とし
て、炭素数14、16および／または18の脂肪酸のカルシウ
ム、マグネシウム等の２価金属塩が提案され（USP 4,82
6,694明細書参照）、天然油脂から製造された混合脂肪
酸のカルシウム塩（以下「バイパス油脂」と称す）が市
販されている。On the other hand, divalent metals such as calcium, magnesium and the like of fatty acids having 14, 16, and / or 18 carbon atoms are used as an energy source that efficiently bypasses the rumen of ruminants and is absorbed in the digestive organs of the abomasum and beyond with high efficiency. Salt is proposed (USP 4,82
6,694), and calcium salts of mixed fatty acids produced from natural fats and oils (hereinafter referred to as “bypass fats and oils”) are commercially available.
またこれらのバイパス油脂をその融点以上の温度に加
熱して軟化し、その中に生物学的活性物質を添加混合し
た後、冷却固化して粉砕する反芻動物用飼料添加剤の製
造法が知られている（特開昭63−313546号公報参照）。Also known is a method for producing a feed additive for ruminant animals in which these bypass fats are heated to a temperature above their melting point to soften, and a biologically active substance is added and mixed therein, then cooled and solidified and pulverized. (See JP-A-63-313546).
前記引用した硬化油等の保護物質で生物学的活性物質
を被覆保護した反芻動物用飼料添加剤においては、生物
学的活性物質の第１胃バイパス性および第４胃以降の消
化器官での放出性が優れているが、貯蔵安定性、特に40
℃以上で保護した場合の熱安定性を改良することが要求
されている。In the feed additive for ruminants in which the biologically active substance is coated and protected with the above-mentioned protective substances such as hardened oil, the ruminal bypass of the biologically active substance and the release in the digestive tract after the abomasum are performed. Good storage stability, especially 40
There is a need to improve the thermal stability when protected above ℃.
一方、バイパス油脂は、硬化油等に比較して融点が高
く、熱安定性に優れている。したがって、生物学的活性
物質をバイパス油脂で被覆保護した製剤は熱安定性に優
れることが期待できるが、バイパス油脂は結晶性であ
り、前期引用した方法でバイパス油脂のみを用いて製造
した製剤においては緻密な保護被覆が形成されにくく製
剤の性能、すなわち生物学的活性物質の第１胃バイパス
性にバラツキがある。また、生物学的活性物質を高含有
させた製剤や、水溶性の高い生物学的活性物質を含有す
る製剤においては、バイパス油脂のみからなる保護物質
では生物学的活性物質の十分な被覆が困難であることか
ら、生物学的活性物質の第１胃バイパス性がほとんど得
られない。On the other hand, bypass fats and oils have a higher melting point than hardened oils and the like, and are excellent in thermal stability. Therefore, a formulation in which a biologically active substance is covered and protected with bypass oils and fats can be expected to have excellent thermal stability, but bypass oils and fats are crystalline. Is difficult to form a dense protective coating, and the performance of the preparation, that is, the ruminal bypass of biologically active substances varies. In addition, in a preparation containing a high content of a biologically active substance or a preparation containing a biologically active substance with high water solubility, it is difficult to sufficiently coat the biologically active substance with a protective substance consisting of only a bypass fat. Therefore, the ruminal bypass of the biologically active substance is hardly obtained.
本発明は、生物学的活性物質の第１胃バイパス性、第
４胃放出性および熱安定性に優れた反芻動物用飼料添加
剤を提供することをその目的とする。It is an object of the present invention to provide a ruminant feed additive which is excellent in the ruminal bypass property, abomasum release property and heat stability of a biologically active substance.
本発明は、 （１） 生物学的活性物質を、脂肪酸金属塩単独又は脂
肪酸金属塩を主成分とする保護マトリックス中に分散し
保護した製剤からなり、該製剤の空隙率が15％以下かつ
水分含有量が２重量％以下であることを特徴とする反芻
動物用飼料添加剤、 （２） 前記製剤において、脂肪酸金属塩中の遊離の金
属水酸化物が５重量％以下である反芻動物用飼料添加剤
および、 （３） 前記（１）または（２）の製剤において、脂肪
酸金属塩を主成分とする保護マトリックスが該脂肪酸金
属塩と相溶する水不溶性物質を含有する反芻動物用飼料
添加剤である。The present invention provides: (1) a preparation in which a biologically active substance is dispersed and protected in a protective matrix containing a fatty acid metal salt alone or a fatty acid metal salt as a main component, wherein the porosity of the preparation is 15% or less and the water content is A ruminant feed additive having a content of 2% by weight or less, (2) a ruminant feed in which the free metal hydroxide in the fatty acid metal salt is 5% by weight or less in the formulation. (3) In the preparation of the above (1) or (2), the feed additive for ruminant animals, wherein the protective matrix containing a fatty acid metal salt as a main component contains a water-insoluble substance compatible with the fatty acid metal salt. It is.
本発明において、生物学的活性物質は、動物に供与し
て肥育促進、乳質改善、泌乳量増加、疾病予防、疾病治
療等の活性を示す物質であり、特に反芻動物に直接経口
投与した場合、第１胃において第１胃内に存在する微生
物により分解され易く、そのままでは効力が発現されに
くい物質である。In the present invention, the biologically active substance is a substance that shows an activity of promoting fattening, improving milk quality, increasing milk yield, preventing disease, treating disease, etc. by donating to an animal, particularly when orally administered directly to a ruminant, It is a substance that is easily decomposed in the rumen by microorganisms present in the rumen, and is unlikely to exhibit efficacy as it is.
たとえばアミノ酸類：メチオニン，リジン，トリプト
ファン等、Ｎ−アシルアミノ酸類:N−ステアロイルメチ
オニン,N−オレインメチルオニン,N−ヒドロキシメチル
メチオニンのカルシウム塩等、アミノ酸の塩類：リジン
塩酸塩等、アミノ酸のヒドロキシ同族化合物類:2−ヒド
ロキシ−４−メチルメルカプト酪酸およびそのカルシウ
ム塩等、タンパク質類：魚粉末，カゼイン，馬鈴薯蛋
白，大豆蛋白等、ビタミン類：ビタミンA,ビタミンＡ酢
酸エステル，ビタミンＡパルミチン酸エステル，ビタミ
ンD3,ビタミンE,ニコチン酸およびニコチン酸アミド，
パントテン酸カルシウム，β−カロチン等、酵素類：酸
性プロテアーゼ等、炭水化物類：ぶどう糖等、獣医薬
類：ペニシリン，テトラサイクリン等の抗生物質，ネグ
フォン等の駆虫薬等が挙げられ、それらの１種または２
種以上が使用される。この生物学的活性物質は、製剤の
投与目的により各種含有量のものが調製されるが、過少
な場合飼料添加剤の給与量が非常に多くなり、不都合が
生じる。一方、過大な場合、保護マトリックスによる生
物学的活性物質の十分な保護効果が得られず、従って第
１胃バイパス性が達成されない。従って生物学的活性物
質含有量は２〜40数量％が好ましく、更に好ましくは２
〜30重量％である。For example, amino acids: methionine, lysine, tryptophan, etc. N-acyl amino acids: N-stearoyl methionine, N-olein methylonine, calcium salts of N-hydroxymethyl methionine, etc. Salts of amino acids: lysine hydrochloride, etc. Homologous compounds: 2-hydroxy-4-methylmercaptobutyric acid and its calcium salt, etc. Proteins: fish powder, casein, potato protein, soy protein, etc., vitamins: vitamin A, vitamin A acetate, vitamin A palmitate , vitamin D 3, vitamin E, nicotinic acid and nicotinamide,
Enzymes: acid protease, etc. Carbohydrates: glucose, etc. Veterinary drugs: Antibiotics such as penicillin and tetracycline, anthelmintics such as Negphone, etc., and one or two of them.
More than seeds are used. This biologically active substance is prepared in various contents depending on the purpose of administration of the preparation. However, if the content is too small, the feeding amount of the feed additive becomes extremely large, which causes inconvenience. On the other hand, if it is too large, the protective matrix does not provide a sufficient protective effect of the biologically active substance, so that the rumen bypass property cannot be achieved. Therefore, the content of the biologically active substance is preferably 2 to 40% by volume, more preferably 2% by volume.
~ 30% by weight.
脂肪酸金属塩は、たとえば炭素数８〜22の直鎖または
分岐を有する飽和または不飽和の脂肪酸の金属塩、好ま
しくは２価の金属塩であり、さらに好ましくはカルシウ
ム塩である。また前記引用した天然油脂から製造される
炭素数14、16および／または18の混合脂肪酸のカルシウ
ム塩等も使用できる。The fatty acid metal salt is, for example, a saturated or unsaturated fatty acid metal salt having a straight or branched chain having 8 to 22 carbon atoms, preferably a divalent metal salt, and more preferably a calcium salt. In addition, calcium salts of mixed fatty acids having 14, 16 and / or 18 carbon atoms produced from the above-mentioned natural fats and oils can also be used.
好ましくは融点が30〜50℃、さらに好ましくは35〜45
℃の混合脂肪酸のカルシウム塩を使用する。Preferably the melting point is 30-50 ° C, more preferably 35-45
Use the calcium salt of mixed fatty acids at 0 ° C.
また前記脂肪酸金属塩と相溶する水不溶性物質とし
て、たとえば炭素数８〜22の飽和または不飽和の直鎖ま
たは分岐を含有する脂肪酸類、高級アルコール類、グリ
セリンモノ脂肪酸エステル類等が使用される。特に融点
が20〜80℃の範囲にある脂肪酸類、高級アルコール類、
グリセリンモノ脂肪酸エステル類およびそれらの混合物
が使用される。これら脂肪酸金属塩と相溶する水不溶性
物質の含有量は該脂肪酸金属塩を相溶するに十分な量で
あればよく、通常、相溶性物質／脂肪酸金属塩（重量基
準）として5/95〜25/75である。相溶性物質の過剰な配
合は、製剤の融点を低下させるので好ましくない。Further, as the water-insoluble substance compatible with the fatty acid metal salt, for example, fatty acids, higher alcohols, glycerin monofatty acid esters and the like having a saturated or unsaturated straight or branched chain having 8 to 22 carbon atoms are used. . In particular, fatty acids and higher alcohols having melting points in the range of 20 to 80 ° C,
Glycerin monofatty acid esters and mixtures thereof are used. The content of the water-insoluble substance compatible with the fatty acid metal salt may be a sufficient amount to be compatible with the fatty acid metal salt, and is usually 5/95 to 5% by weight as the compatible substance / fatty acid metal salt. 25/75. Excessive incorporation of a compatible substance is not preferred because it lowers the melting point of the preparation.
また、第１胃バイパス性をさらに向上させるために、
硬化した動植物油、ワックス等を添加することができ
る。これらの添加割合については特に制限はないが、保
護物質としての融点が、60℃以上であることが好まし
く、特に80℃以上が好ましい。In addition, in order to further improve the rumen bypass property,
Hardened animal and vegetable oils, waxes and the like can be added. Although there is no particular limitation on the ratio of these additions, the melting point as a protective substance is preferably 60 ° C. or higher, particularly preferably 80 ° C. or higher.
本発明の製剤は、生物学的活性物質の第４胃における
溶出性をさらに向上させるために、中性域では不溶性で
あり、酸性域において膨潤、溶解または分解性を示す崩
壊性付与剤を添加することができる。このような崩壊性
付与剤として、たとえばキトサンが挙げられる。The formulation of the present invention contains a disintegrating agent which is insoluble in the neutral region and swells, dissolves or decomposes in the acidic region in order to further improve the dissolution of the biologically active substance in the abomasum. can do. Examples of such a disintegrating agent include chitosan.
さらに製剤の比重を調節する目的で、炭酸カルシウム
のような無機フィラーを添加することもできる。Further, for the purpose of adjusting the specific gravity of the preparation, an inorganic filler such as calcium carbonate can be added.
本発明では、製剤の空隙率は15％以下である。空隙率
が15％以下の場合には生物学的活性物質は反芻動物の第
１胃をバイパスするが、15％より大きい場合には製剤へ
の水の進入度が大きく生物学的活性物質の第１胃バイパ
ス性が劣る。また、空隙率が15％以下であれば製剤が咀
嚼されても生物学的活性物質の第１胃バイパス性は維持
される。そして、空隙率が15％より大きくなると製剤の
保存、運搬中の粉化率が大きくなり製剤形が保てない。In the present invention, the porosity of the preparation is 15% or less. When the porosity is less than 15%, the biologically active substance bypasses the rumen of the ruminant. Poor gastric bypass. If the porosity is 15% or less, the rumen bypass of the biologically active substance is maintained even when the preparation is chewed. If the porosity is larger than 15%, the powdering rate during storage and transportation of the preparation increases, and the preparation form cannot be maintained.
さらに本発明では、製剤の水分含有量は２重量％以下
である。これより水分含有量が大きいと生物学的活性物
質の第４胃以降の消化器官での放出性が低下し、水分含
有量を２重量％以下に抑えることで良好な性能の製剤を
安定に製造できる。Furthermore, in the present invention, the water content of the preparation is 2% by weight or less. If the water content is higher than this, the release of the biologically active substance from the digestive tract after the abomasum is reduced, and the water content is suppressed to 2% by weight or less, whereby a preparation with good performance is stably manufactured. it can.
また本発明の製剤は、好ましくは脂肪酸金属塩中の遊
離の金属水酸化物が５重量％以下である。この範囲では
生物学的活性物質の第４胃以降の消化器官での放出性は
いっそう良好である。In the preparation of the present invention, the free metal hydroxide in the fatty acid metal salt is preferably 5% by weight or less. In this range, the release of the biologically active substance in the digestive tract after the abomasum is even better.
本製剤は、前記脂肪酸金属塩、脂肪酸金属塩と相溶す
る水不溶性物質、場合によりその他の添加物および生物
学的活性物質を混合し、加熱軟化して混練後、成形する
ことにより容易に製造できる。This preparation is easily manufactured by mixing the above-mentioned fatty acid metal salt, a water-insoluble substance compatible with the fatty acid metal salt, and optionally other additives and a biologically active substance, heating and softening and kneading, followed by molding. it can.
本製剤の成形法には、押し出し造粒法等を採用するこ
とができ、製造装置には通常の熱可塑性樹脂用の押し出
し機等が好適に使用される。ただし、押し出し造粒法を
採用した場合、混練物の脱気が不十分であると、空隙
率、水分含有量が共に大きくなり、生物学的活性物質の
溶出特性が低下する。An extrusion granulation method or the like can be adopted as a molding method of the present preparation, and an ordinary extruder for a thermoplastic resin or the like is suitably used for the production apparatus. However, when the extrusion granulation method is adopted, if the deaeration of the kneaded material is insufficient, both the porosity and the water content increase, and the elution characteristics of the biologically active substance decrease.
本発明において、保護物質の成分である脂肪酸金属塩
が、中性域では不溶性かつ酸性域では分解性であること
から、pH5〜８の範囲にある反芻動物の第１胃の胃液に
極めて安定であり、pH3以下の反芻動物の第４胃で容易
に分解する。その結果生物学的活性物質が反芻動物の第
４胃で溶出し、それ以降の消化器官で効率よく吸収され
る。In the present invention, since the fatty acid metal salt, which is a component of the protective substance, is insoluble in the neutral region and degradable in the acidic region, it is extremely stable in the rumen gastric juice of ruminants in the pH range of 5 to 8. Yes, readily decomposes in the rumen of ruminants below pH 3. As a result, the biologically active substance elutes in the rumen of the ruminant and is efficiently absorbed by the digestive tract thereafter.
さらに保護物質の成分として、前記脂肪酸塩と相溶す
る水不溶性の高級脂肪酸類、高級アルコール類、グリセ
リンモノ脂肪酸エステル類等を併用した場合には、本質
的に結晶性である脂肪酸金属塩単独で成形するよりも緻
密な保護物質が得られ、生物学的活性物質の第１胃バイ
パス性が安定する。これは、脂肪酸金属塩と相溶する水
不溶性物質を添加することにより混練物（製剤）の融点
が若干低下するものの、脂肪酸金属塩の結晶性がなくな
り、緻密な保護マトリックスが形成され、生物学的活性
物質を保護する性能が向上するのであると考えられる。Furthermore, when water-insoluble higher fatty acids compatible with the fatty acid salt, higher alcohols, glycerin monofatty acid esters and the like are used in combination as the components of the protective substance, the fatty acid metal salt which is essentially crystalline alone is used. A denser protective substance is obtained than by molding, and the ruminal bypass of the biologically active substance is stabilized. This is because although the addition of a water-insoluble substance compatible with the fatty acid metal salt slightly lowers the melting point of the kneaded product (formulation), the crystallinity of the fatty acid metal salt is lost, and a dense protective matrix is formed. It is thought that the performance of protecting the active substance is improved.
また生物学的活性物質の保護物質が緻密な被覆である
ため、生物学的活性物質を高含有する製剤や、水溶性の
高い生物学的活性物質を含有する製剤においても優れた
第１胃バイパス性が得られる。In addition, since the protective substance of the biologically active substance is a dense coating, the ruminal bypass is excellent even in a preparation containing a high amount of the biologically active substance or a preparation containing the biologically active substance having high water solubility. Property is obtained.
さらに保護物質の融点が60℃以上であることから、優
れた保存安定性、特に熱安定性が得られる。Further, since the melting point of the protective substance is 60 ° C. or more, excellent storage stability, particularly heat stability can be obtained.
本発明を、実施例および比較例によりさらに詳細に説
明する。The present invention will be described in more detail with reference to Examples and Comparative Examples.
ただし、本発明の範囲は、以下の実施例により何等の
制限を受けるものではない。However, the scope of the present invention is not limited by the following examples.
なお、以下の例中において、「部」および「％」は、
特に断りのない限り重量基準である。In the following examples, "parts" and "%"
Unless otherwise specified, it is based on weight.
（１） 各種製剤の調製 各種生物学的活性物質、第１表に示した混合脂肪酸の
Ca塩および各種添加物質を混合し、真空脱気装置付の押
出成形機を使用して直径２〜3mmの紐状に押し出し、長
さ２〜3mmに切断し、本発明の製剤：飼料MA−１〜MA−1
6（メチオニン含有製剤）、LA−１〜LA−14（リジン塩
酸塩含有製剤）ならびにVA−１〜VA−10（ビタミンＥ含
有製剤）および比較のための製剤：飼料MC−１〜MC−５
（メチオニン含有製剤）ならびにLC−１〜LC−３（リジ
ン塩酸塩含有製剤）を調製した。水分含有率及び空隙率
の違いは押出機のベント部の真空度を変えて調製した。(1) Preparation of various preparations Various biologically active substances, mixed fatty acids shown in Table 1
The Ca salt and various additives are mixed, extruded into a string having a diameter of 2 to 3 mm using an extruder equipped with a vacuum deaerator, cut into a length of 2 to 3 mm, and the preparation of the present invention: feed MA- 1 to MA-1
6 (methionine-containing preparation), LA-1 to LA-14 (lysine hydrochloride-containing preparation) and VA-1 to VA-10 (vitamin E-containing preparation) and preparations for comparison: feed MC-1 to MC-5
(Methionine-containing preparation) and LC-1 to LC-3 (lysine hydrochloride-containing preparation) were prepared. The difference in water content and porosity was prepared by changing the degree of vacuum at the vent of the extruder.
また比較として、生物学的活性物質と牛脂硬化油とを
混合し、要すればキトサンを添加して加熱溶融して噴霧
造粒し、平均粒径が1mmφの真球状の牛脂硬化油を保護
マトリックスとする製剤：試料CC−１〜CC−６を調製し
た。As a comparison, a biologically active substance and hardened tallow oil are mixed, and if necessary, chitosan is added and melted by heating to spray granulate, and a hard spherical tallow oil having an average particle diameter of 1 mmφ is used as a protective matrix. Preparation: Samples CC-1 to CC-6 were prepared.
得られた製剤の組成および物性を第２表に示した。 Table 2 shows the composition and physical properties of the obtained preparation.
第２表中、各項目は、下記に基づいた。 In Table 2, each item was based on the following.
（ａ）混合脂肪酸Ca塩中の遊離Ca（OH）２量： 室温で水に遊離Ca（OH）２を抽出し、EDTA滴定法によ
り測定 （ｂ）混合脂肪酸Ca塩を構成する脂肪酸の融点： メーカー分析値より析出 （ｃ）空隙率： 空隙率＝［（W0−W1）/W0］×100 W0:製剤の真比重 W1:得られた製剤の比重 （ｄ）水分含有量： 乾燥減量法（110℃×４時間）および示差熱分析法に
より測定 また、第２表中、混合脂肪酸Ca塩溶解性物質および添
加物の種類として下記の記号を用いた。(A) Amount of free Ca (OH) 2 in mixed fatty acid Ca salt: Extraction of free Ca (OH) 2 in water at room temperature and measurement by EDTA titration method (b) Melting point of fatty acid constituting mixed fatty acid Ca salt: (C) Porosity: Porosity = [(W 0 −W 1 ) / W 0 ] × 100 W 0 : True specific gravity of the preparation W 1 : Specific gravity of the obtained preparation (d) Water content : Measured by loss on drying method (110 ° C. × 4 hours) and differential thermal analysis. In Table 2, the following symbols were used as the types of the mixed fatty acid Ca salt-soluble substance and additives.
HHA:ひまし油硬化脂肪酸 YA :やし油脂肪酸 NHA:なたね油硬化脂肪酸 CHT:キトサン （２） 各種製剤の評価試験 前記第（１）項で調製した各製剤について、それぞれ
2gを牛の第一胃胃液に対応したTris緩衝液:200ccに浸漬
し、37℃の温度下に24時間振盪保持した。ついで飼料を
Tris緩衝液から取り出し牛の第四胃胃液に対応した0.05
M（＝mol・dm-3）塩酸:200ccに浸漬し、37℃の温度下に
さらに４時間振盪保持した。引続き0.05M塩酸から取り
出した試料を、牛の小腸液対応液:200ccに浸漬し、37℃
の温度下にさらに４時間振盪保持した。HHA: Castor oil-cured fatty acid YA: Palm oil fatty acid NHA: Rapeseed oil-cured fatty acid CHT: Chitosan (2) Evaluation test of various preparations
2 g of the bovine ruminal fluid was immersed in 200 cc of a Tris buffer solution corresponding to the rumen gastric juice, and kept under shaking at a temperature of 37 ° C. for 24 hours. Then feed
0.05 from bovine abomasum gastric juice removed from Tris buffer
M (= mol · dm −3 ) hydrochloric acid: immersed in 200 cc and shaken and maintained at a temperature of 37 ° C. for 4 hours. Subsequently, the sample taken out from 0.05M hydrochloric acid was immersed in a small intestinal fluid-compatible solution of cattle: 200 cc,
At 4 ° C. for a further 4 hours.
＜Tris緩衝液＞ Tris［トリス（ヒドロキシメチル）アミノメタン］:6.0
6gを292mlの0.1M塩酸に溶解し、水で1,000mlに希釈した
pH8.0の溶液 前記各対応液に溶出した生物学的活性物質量および脂
肪酸Ca塩が解離して生成したCaイオン量を、下記の方法
により測定した。<Tris buffer> Tris [tris (hydroxymethyl) aminomethane]: 6.0
6 g was dissolved in 292 ml of 0.1 M hydrochloric acid and diluted to 1,000 ml with water.
pH 8.0 solution The amount of the biologically active substance eluted in each of the corresponding solutions and the amount of Ca ions generated by dissociation of the fatty acid Ca salt were measured by the following methods.
（ａ） リジン ：ニンヒドリン発色法により測定 （ｂ） メチオニン：ヨード滴定法により測定 （ｃ） ビタミン類：高速液体クロマトグラフィーを用
いて測定 （ｄ） 解離した脂肪酸Ca塩：解離した生成したCaイオ
ンを、EDTA滴定法により測定 各測定結果を第３表に示す。(A) Lysine: Measured by ninhydrin colorimetric method (b) Methionine: Measured by iodometric titration (c) Vitamins: Measured by high performance liquid chromatography (d) Dissociated fatty acid Ca salt: Dissociated Ca ions formed Table 3 shows the results of the measurements.
〔発明の硬化〕 本発明の反芻動物用飼料添加剤は、前記実施例にも示
したように、反芻動物に経口投与した場合に、それに含
まれる生物学的活性物質の第１胃バイパス性および第４
胃以降の消化器官での溶出特性が極めて安定でかつ優れ
ており、また高い耐熱性も有することから、保存安定性
にも極めて優れている。 [Curing of the Invention] The ruminant feed additive of the present invention, as shown in the above Examples, has a ruminal bypass property of a biologically active substance contained therein when administered orally to ruminants. 4th
The dissolution characteristics in the digestive tract after the stomach are extremely stable and excellent, and since it also has high heat resistance, it is extremely excellent in storage stability.
本発明は、経口投与した場合に反芻動物の第１胃で分
解されやすい生物学的活性物質を、第１胃をバイパスさ
せ第４胃以降の消化器官で高効率で吸収させるに好適
な、かつ保存安定性、特に熱安定性の優れた反芻動物用
飼料添加剤を提供するものであり、その産業上、特に畜
産分野における意義は極めて大きい。The present invention is suitable for allowing a biologically active substance which is liable to be degraded in the rumen of a ruminant animal when orally administered to the animal to bypass the rumen and to be absorbed with high efficiency in the digestive organs after the abomasum, and An object of the present invention is to provide a ruminant feed additive having excellent storage stability, particularly heat stability, and its significance is extremely large in the industry, particularly in the livestock industry.
───────────────────────────────────────────────────── フロントページの続き (58)調査した分野(Int.Cl.6，ＤＢ名) A23K 1/18 A23K 1/16──────────────────────────────────────────────────続 き Continued on the front page (58) Field surveyed (Int.Cl. 6 , DB name) A23K 1/18 A23K 1/16
Claims (3)
は脂肪酸金属塩を主成分とする保護マトリックス中に分
散し保護した製剤からなり、該製剤の空隙率が15％以下
かつ水分含有量が２重量％以下であることを特徴とする
反芻動物用飼料添加剤1. A preparation in which a biologically active substance is dispersed and protected in a protective matrix containing a fatty acid metal salt alone or a fatty acid metal salt as a main component, wherein the porosity of the preparation is 15% or less and the water content is Characterized in that the content is less than 2% by weight.
中の遊離の金属水酸化物が５重量％以下である反芻動物
用飼料添加剤2. The feed additive for ruminant animals according to claim 1, wherein the free metal hydroxide in the fatty acid metal salt is 5% by weight or less.
て、脂肪酸金属塩を主成分とする保護マトリックスが、
該脂肪酸金属塩と相溶する水不溶性物質を含有する反芻
動物用飼料添加剤3. The protective matrix according to claim 1 or 2, wherein the protective matrix containing a fatty acid metal salt as a main component is:
Ruminant feed additive containing a water-insoluble substance compatible with the fatty acid metal salt
Priority Applications (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2082552A JP2847882B2 (en) | 1990-03-29 | 1990-03-29 | Ruminant feed additives |
| ES91905311T ES2059126T3 (en) | 1990-03-02 | 1991-03-02 | FOOD ADDITIVE FOR RUMINANTS. |
| DK91905311.6T DK0478783T3 (en) | 1990-03-02 | 1991-03-02 | Feed additive for ruminants |
| AT91905311T ATE107476T1 (en) | 1990-03-02 | 1991-03-02 | FEED SUPPLEMENTS FOR RUMINANTS. |
| PCT/JP1991/000282 WO1991012731A1 (en) | 1990-03-02 | 1991-03-02 | Feed additive for ruminant |
| AU73115/91A AU635823B2 (en) | 1990-03-02 | 1991-03-02 | Ruminant feed additive for rumen bypass |
| EP91905311A EP0478783B1 (en) | 1990-03-02 | 1991-03-02 | Feed additive for ruminant |
| CA002051422A CA2051422C (en) | 1990-03-02 | 1991-03-02 | Feedstuffs for ruminants |
| DE69102608T DE69102608T2 (en) | 1990-03-02 | 1991-03-02 | FEEDING ADDITIVES FOR Ruminants. |
| FI915180A FI915180A0 (en) | 1990-03-02 | 1991-11-01 | FODERTILLSATS FOER IDISSLARE. |
| NO914301A NO301573B1 (en) | 1990-03-02 | 1991-11-01 | Feed for ruminants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2082552A JP2847882B2 (en) | 1990-03-29 | 1990-03-29 | Ruminant feed additives |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH03280845A JPH03280845A (en) | 1991-12-11 |
| JP2847882B2 true JP2847882B2 (en) | 1999-01-20 |
Family
ID=13777664
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2082552A Expired - Lifetime JP2847882B2 (en) | 1990-03-02 | 1990-03-29 | Ruminant feed additives |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2847882B2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101467592A (en) * | 2007-12-29 | 2009-07-01 | 农村振兴厅 | Ruminant feed addictive for protecting vitamin C as well as preparation method and application thereof |
-
1990
- 1990-03-29 JP JP2082552A patent/JP2847882B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH03280845A (en) | 1991-12-11 |
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