JP2929812B2 - Ruminant feed additive composition - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a feed additive composition for ruminants. More specifically, the biologically active substance is coated with a coating composition that is stable in the rumen of the ruminant animal and allows the release of the biologically active substance in the digestive tract below the abomasum. The present invention relates to a ruminant feed additive composition which is coated and enables digestion of biologically active substances in the digestive tract below the abomasum.

[0002]

2. Description of the Related Art In ruminants such as cows and sheep, amino acids,
Oral administration of biologically active substances such as vitamins directly
Most are degraded by microorganisms in the rumen and are not effectively used.

[0003] Therefore, these biologically active substances are
A ruminant bypass preparation for ruminants that protects against microbial degradation in the rumen and is digested and absorbed in the digestive tract below the abomasum is used in the fields of ruminant feed, nutrients, veterinary drugs, etc. is important.

[0004] As a ruminant feed additive containing a biologically active substance, coating with fatty acids, hardened animal and vegetable oils and the like has been proposed, but particles coated with these fats and oils are the first. While good protection in the stomach, it is difficult to elute biologically active substances in the digestive tract below the abomasum.

[0005] Therefore, a substance other than fats and oils for promoting dissolution is added to the substance to be protected, the biologically active substance is dispersed and granulated, and the biologically active substance is coated with the protective substance. A method of doing so has been proposed.

As a method of dispersing a biologically active substance in a protective substance, for example, JP-A-60-168351 discloses a method of dispersing a biologically active substance, calcium carbonate at 20% by weight or more, an aliphatic monocarboxylic acid, and a hardened fat or oil. And the like are proposed in which 10% by weight or more is contained and granulation is carried out. In addition, Japanese Unexamined Patent Publication
In 1-195563, a biologically active substance is dispersed in a protective substance comprising 10% by weight or more of an aliphatic monocarboxylic acid, a hardened oil or the like, and 20% by weight or more and 50% by weight or less of an insoluble salt of an acid weakly acidic than hydrochloric acid. Suggest a way to do it.

As a method of coating a biologically active substance with a protective substance, for example, JP-A-63-317053 discloses a method of coating a biologically active substance with a protective agent comprising an aliphatic monocarboxylic acid, a hardened oil and lecithin and a glycerin fatty acid ester. A method of coating the material is proposed.

However, in the method of dispersing a biologically active substance in a protective substance, the biologically active substance is present near the particle surface. It is difficult to sufficiently protect the water-soluble biologically active substance, considering that the residence time in the rumen is 10 to several hours to several days. Further, when coated with a protective substance consisting of lecithin and glycerin fatty acid ester and fats and oils, the strength of the coating layer is insufficient and a problem remains in the protective property. Although lecithin and glycerin fatty acid esters are expected to emulsify fats and oils, they cannot be said to have sufficient dissolution properties in view of the time required to pass through the digestive tract after the abomasum.

In addition, in order to utilize the pH difference between the rumen and the abomasum, a method of coating with a pH-responsive synthetic polymer has also been proposed. In view of the fact that it becomes expensive, it cannot be said that this is a sufficiently satisfactory means.

[0010]

The object of the present invention is to stably protect a biologically active substance in the rumen of a ruminant animal in consideration of safety and economy. The purpose is to efficiently digest and absorb the gastrointestinal tract below the abomasum.

[0011]

The present inventors have made intensive efforts to achieve the above-mentioned object, and as a result, the nucleus containing the biologically active substance is stable in lecithin and neutral,
14 carbon atoms containing inorganic substances soluble under acidic conditions
At least one selected from the group consisting of linear or branched saturated or unsaturated aliphatic monocarboxylic acids or salts thereof having up to 22 carbon atoms, hardened vegetable oils, hardened animal oils and waxes, and waxes The present invention has been achieved by finding that by coating with various kinds of protective substances, it is possible to have both excellent protection in the rumen and excellent dissolution in the lower digestive tract below the abomasum.

That is, the gist of the present invention is that a nucleus containing a biologically active substance comprises a linear or branched saturated or unsaturated aliphatic monocarboxylic acid having 14 to 22 carbon atoms, At least one substance selected from the group consisting of salts, hardened vegetable oils, hardened animal fats and waxes, and waxes, lecithin, and one of inorganic substances that are stable in neutral but soluble under acidic conditions. A feed additive composition for ruminants, which is coated with a coating composition containing at least one species.

The ruminant feed additive composition of the present invention contains lecithin and a neutrally stable protective substance in a protective substance, but contains an inorganic substance soluble under acidic conditions, but is acidic in the abomasum. With the release property by the action of inorganic substances utilizing that and the emulsifying action of fatty acids, hardened fats etc. by lecithin,
It also has the property of releasing a biologically active substance in the lower digestive tract from the abomasum and has a good dissolution property due to its synergistic effect. Further, the strength of the coating layer is increased by the addition of the inorganic salt. Hereinafter, the present invention will be described in detail.

In the present invention, the biologically active substance includes various known nutrients and feeds or drugs containing the same, for example, amino acids and their derivatives, hydroxy homologous compounds of amino acids, proteins, carbohydrates, vitamins. Or a mixture of two or more selected from the group and veterinary medicine.

More specifically, amino acids such as lysine, methionine, tryptophan and threonine; amino acid derivatives such as N-acylamino acids, calcium salts of N-hydroxymethylmethionine and lysine hydrochloride; 2-hydroxy-4-methylmercapto Hydroxy homologous compounds of amino acids such as butyric acid and salts thereof; powders of natural nutrients such as cereal powder, feather powder, fish meal; proteins such as casein, corn protein, potato protein; carbohydrates such as starch, sucrose, glucose; , Vitamin A acetate,
Vitamin A palmitate, vitamin B group, thiamine,
Thiamine hydrochloride, riboflavin, nicotinic acid, nicotinamide, calcium pantothenate, choline pantoate, pyridoxine hydrochloride, choline chloride, cyanocobalamin, biotin, folic acid, p-aminobenzoic acid, vitamin D
2. Vitamins such as vitamin D3 and vitamin E and substances having functions similar thereto; tetracyclines, aminoglycosides, macrolides, polyether antibiotics, anthelmintics such as negphone, insecticides such as piperazine, estrogen Hormones such as stilbestrol, hexestrol, thyroprotein and goitrogen are used.

The method for preparing the core containing the biologically active substance is not particularly limited, and if necessary, a thickener, an excipient, etc. may be added, and a conventional granulation method such as extrusion granulation, fluidization Granular, preferably nearly spherical, particles are prepared by a granulation method, a stirring granulation method or the like.

Examples of the thickener include cellulose derivatives such as hydroxypropylcellulose, methylcellulose and sodium carboxymethylcellulose, vinyl derivatives such as polyvinyl alcohol and polyvinylpyrrolidone, gum arabic, guar gum, sodium polyacrylate and the like.

As the excipient, starch, protein, crystalline cellulose and the like can be used. If necessary, calcium carbonate, calcium phosphate, talc or the like may be added as a specific gravity adjuster.

The protective substance for coating the nucleus containing the biologically active substance is a linear or branched saturated or unsaturated aliphatic monocarboxylic acid having 14 to 22 carbon atoms or a salt thereof, Hardened vegetable oils, hardened animal fats and waxes, at least one substance selected from the group consisting of waxes, lecithin, and one of inorganic substances that are stable in neutral but soluble under acidic conditions or Contains two or more.

As the aliphatic monocarboxylic acid, myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, behenic acid and the like can be used, and their salts may be used. As hardened vegetable oils, hardened palm oil, hardened soybean oil, hardened rapeseed oil, hardened castor oil, etc., as hardened animal oils, hardened tallow, hardened lard, etc., as waxes, carnauba wax, beeswax, etc., as waxes Can be natural wax, synthetic wax, paraffin wax or the like.

The lecithin used in the present invention does not necessarily have to be pure, but may be a mixture of phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, etc., and is preferably produced using soybean, egg yolk or the like as a raw material. Commercially available lecithin is used.

Inorganic substances which are stable under neutral conditions but are soluble under acidic conditions include magnesium carbonate, calcium carbonate, calcium phosphate, calcium pyrophosphate and the like. Carbonates such as magnesium carbonate and calcium carbonate, and pyrophosphate Is more preferred.

The composition of the protective substance in the present invention is 0.1 to 20% by weight of lecithin in the total protective substance, and is neutral and stable;
0.1 to 10% by weight of an inorganic substance soluble under acidic conditions, preferably 1 to 10% of lecithin and 1 to 10% of an inorganic substance.
%. If the lecithin content in the protective substance exceeds 20% by weight, the strength of the coating layer is reduced and the protective properties in the rumen are reduced. If the lecithin content is less than 0.1% by weight, the emulsifying action is insufficient and the dissolution in the lower digestive tract from the abomasum is reduced. Neutral and stable, when the amount of the inorganic substance soluble under acidic conditions exceeds 10% by weight, the protective property in the rumen decreases,
If it is less than 0.1%, the action of the inorganic substance utilizing the fact that the inside of the abomasum is acidic is insufficient.

The ruminant feed additive composition of the present invention is characterized in that a core containing the biologically active substance is coated with the protective substance.

There is no particular limitation on the coating amount of the protective substance, and it is preferable that the coating amount of the protective substance is as small as possible because the content of the biologically active substance is large. However, an amount that can sufficiently protect the biologically active substance in the rumen is required. Usually, 10 to 200 parts by weight, preferably 15 to 150 parts by weight is coated with respect to 100 parts by weight of the core containing the biologically active substance.

There is no particular limitation on the coating method, and ordinary coating methods such as a fluid coating method, a pan coating method,
It can be coated by a melt coating method or the like.

Hereinafter, the present invention will be described in more detail with reference to Examples and Comparative Examples, but the scope of the present invention is not limited to these Examples.

[0028]

EXAMPLES The usefulness of ruminant feed additives is as follows.
Evaluation was made by the following method.

About 2 g of the prepared sample was put into a 200 ml Erlenmeyer flask, and 100 ml of Mc Dougall buffer solution corresponding to the rumen fluid was added.
And shaken at a temperature of 39 ° C. for 48 hours. After shaking, the amount of the biologically active substance eluted was analyzed to calculate the ruminal protection.

The amount of the amino acid eluted in the examples as a biologically active substance was analyzed by liquid chromatography.

* Mc Dougall buffer: a buffer obtained by dissolving the following reagents in 1000 ml of water. Sodium hydrogen carbonate: 7.43 g Disodium phosphate / 12 hydrate: 7.00 g Sodium chloride: 0.34 g Potassium chloride: 0.43 g Magnesium chloride / hexahydrate: 0.10 g Calcium chloride: 0.05 g

Dissolution in the abomasum After completion of the protection test, the shaken sample was recovered, and the adherent solution was washed, and then poured into a 200 ml Erlenmeyer flask.
Inject 40 ml of Clark-Lubs buffer equivalent to gastric juice,
Shake at a temperature of 39 ° C. for 3 hours. After the shaking, the elution amount of the biologically active substance was analyzed, and the elution property of the abomasum was calculated.

* Clark-Lubs buffer: a buffer in which the following reagents are dissolved in 1000 ml of water: potassium chloride: 3.73 g hydrochloric acid: 2.1 ml

Dissolution property in small intestine After the dissolution test in the abomasum was completed, the shaken sample was collected, poured into a 200 ml Erlenmeyer flask, and 100 ml of a buffer solution corresponding to the small intestine fluid was injected. Shake at temperature for 24 hours. After completion of the shaking, the elution amount of the biologically active substance was analyzed, and the elution property of the small intestine was calculated.

[0035]

Example 1 325 g of L-lysine hydrochloride, talc 17
2.5 g, sodium carboxymethyl cellulose 2.
5 g and 135 g of water were charged into a kneader and kneaded.
Columnar granules were obtained using an extrusion granulator having a 1.5 mmφ aperture screen. The granules obtained were converted into granules having a near spherical shape using a sphering apparatus (Malmerizer, manufactured by Fuji Paudal). The obtained spherical granules were flow-dried to obtain a core containing L-lysine hydrochloride.

5 parts by weight of lecithin (using soybean lecithin manufactured by Wako Pure Chemicals, a food additive, the same applies hereinafter) and 5 parts by weight of magnesium carbonate are dispersed in 90 parts by weight of molten hardened tallow, and 100 parts by weight of core The core was coated at a rate of 67 parts by weight (40% coverage). The coating was melt-coated using a fluidized-granulation coating device (New Melmizer, manufactured by Fuji Paudal).

The coated particles were subjected to the above evaluation test. As a result, the ruminal dissolution rate was 9%, the abomasum dissolution rate was 39%, and the small intestine dissolution rate was 40%.

[0038]

Example 2 Coated particles were prepared in the same manner as in Example 1 except that calcium carbonate was used instead of magnesium carbonate as an inorganic substance which was neutral, stable and soluble under acidic conditions.

The coated particles were subjected to the above evaluation test. As a result, the ruminal dissolution rate was 4%, the abomasum dissolution rate was 46%, and the small intestine dissolution rate was 36%.

[0040]

Example 3 Coated particles were prepared in the same manner as in Example 2, except that the coating of the protective substance was carried out at a rate of 43 parts by weight (coverage 30%).

The coated particles were subjected to the above evaluation test. As a result, the ruminal dissolution rate was 12%, the abomasum dissolution rate was 20%, and the small intestine dissolution rate was 58%.

[0042]

Example 4 As a protective substance, 10 parts by weight of lecithin and 5 parts by weight of calcium carbonate were contained with respect to 85 parts by weight of hardened tallow, and the protective substance was coated at a rate of 33 parts by weight with respect to 100 parts by weight of the core. Except for (coverage 25%), coated particles were prepared in the same manner as in Example 2.

The coated particles were subjected to the above evaluation test. As a result, the ruminal dissolution rate was 19%, the abomasum dissolution rate was 46%, and the small intestine dissolution rate was 32%.

[0044]

Example 5 As a protective substance, 2 parts by weight of lecithin and 10 parts by weight of calcium carbonate were contained in 88 parts by weight of hardened beef tallow, and a coating of 25 parts by weight was applied to 100 parts by weight of cores. Except for (20% coverage), coated particles were prepared in the same manner as in Example 2.

The coated particles were subjected to the above evaluation test. As a result, the ruminal dissolution rate was 14%, the abomasum dissolution rate was 31%, and the small intestine dissolution rate was 35%.

[0046]

Example 6 Coated particles were prepared in the same manner as in Example 1, except that calcium pyrophosphate was used instead of calcium carbonate as an inorganic substance which was neutral, stable and soluble under acidic conditions.

The coated particles were subjected to the above evaluation test. As a result, the ruminal dissolution rate was 7%, the abomasum dissolution rate was 49%, and the small intestine dissolution rate was 32%.

[0048]

Example 7 D, L-methionine (375 g), talc 12
0 g, sodium carboxymethylcellulose 5 g, and water 150 g were charged into a kneader, kneaded, and then mixed with 1.5 mm.
Cylindrical granules were obtained using an extrusion granulator having a screen with a mesh opening of φ. The granules obtained were converted into granules having a near spherical shape using a sphering apparatus (Malmerizer, manufactured by Fuji Paudal). The obtained spherical granules are flow-dried, and D, L-
A core containing methionine was obtained.

A protective substance containing 5 parts by weight of lecithin and 5 parts by weight of magnesium carbonate was melted with respect to 90 parts by weight of hardened tallow, and the core was coated at a ratio of 43 parts by weight with respect to 100 parts by weight of the core (coating ratio: 30). %).

The above particles were subjected to the above evaluation test. As a result, the ruminal dissolution rate was 17%, the abomasum dissolution rate was 20%, and the small intestine dissolution rate was 60%.

[0051]

Example 8 Coated particles were prepared in the same manner as in Example 7, except that calcium carbonate was used instead of magnesium carbonate as an inorganic substance which was neutral, stable and soluble under acidic conditions.

The above particles were subjected to the above evaluation test. As a result, the ruminal dissolution rate was 15%, the abomasum dissolution rate was 26%, and the small intestine dissolution rate was 59%.

[0053]

Example 9 As a protective substance, 10 parts by weight of lecithin and 2 parts by weight of calcium carbonate were contained with respect to 88 parts by weight of hardened tallow, and the protective substance was coated at a rate of 33 parts by weight with respect to 100 parts by weight of the core. Coated particles were prepared in the same manner as in Example 8, except that the coating ratio was 25%.

The coated particles were subjected to the above evaluation test. As a result, the ruminal dissolution rate was 21%, the abomasum dissolution rate was 38%, and the small intestine dissolution rate was 37%.

[0055]

Comparative Example 1 Coated particles were prepared in the same manner as in Example 1 except that a protective substance containing 10 parts by weight of calcium carbonate was used for 90 parts by weight of hardened tallow. As a result of performing the above-described evaluation test on the coated particles, the ruminal dissolution rate was 5%, the abomasum dissolution rate was 15%, and the small intestine dissolution rate was 16%.

[0056]

Comparative Example 2 Lecithin 30 was added to 70 parts by weight of hardened tallow.
Coated particles were prepared in the same manner as in Example 3, except that the protective substance contained in parts by weight was used. As a result of performing the above-described evaluation test on the coated particles, the ruminal dissolution rate was 71%, the abomasum dissolution rate was 27%, and the small intestine dissolution rate was 1%.

[0057]

Comparative Example 3 Coated particles were prepared in the same manner as in Example 8, except that a protective substance containing 30 parts by weight of calcium carbonate was used for 70 parts by weight of hardened tallow. As a result of performing the above evaluation test on the coated particles, the ruminal dissolution rate was 82%,
The gastric dissolution rate was 10% and the small intestine dissolution rate was 3%.

The above results are summarized in Tables 1 and 2. It can be seen that the feed additive composition of the present invention has superior effects on the protection in the rumen and the dissolution in the lower digestive tract from the abomasum compared to the conventional composition.

[0059]

[Table 1]

[0060]

[Table 2]

[0061]

As described above, a nucleus containing a biologically active substance is composed of a linear or branched saturated or unsaturated aliphatic monocarboxylic acid having 14 to 22 carbon atoms or a monocyclic or branched aliphatic monocarboxylic acid. At least one substance selected from the group consisting of salts, hardened vegetable oils, hardened animal fats and waxes, and waxes, lecithin, and one of inorganic substances that are stable in neutral but soluble under acidic conditions. A ruminant animal having an effect of being superior in protection in the rumen and dissolution in the lower digestive tract from the abomasum compared to the prior art by being coated with a coating composition containing one or more species. A feed additive composition for use was obtained.

The present invention provides a feed additive which enables a biologically active substance to be effectively absorbed by ruminants, and is of great industrial significance.

Continued on the front page (72) Inventor Takeshi Nagai 1-1 Suzukicho, Kawasaki-ku, Kawasaki-shi, Kanagawa Prefecture Ajinomoto Co., Inc. (72) Inventor Hiroyuki Sato 1-1 Suzukicho, Kawasaki-ku, Kawasaki-shi, Kanagawa Ajino Keiko Nagai, Examiner, Central Research Laboratory, Motoo Corporation (56) References JP-A-63-317053 (JP, A) (58) Fields investigated (Int. Cl. ⁶ , DB name) A23K 1 / 16,1 / 18

Claims (5)

(57) [Claims] 1. A nucleus containing a biologically active substance comprising a linear or branched saturated or unsaturated aliphatic monocarboxylic acid having 14 to 22 carbon atoms or a salt thereof, and a hardened vegetable. At least one substance selected from the group consisting of fats and oils, hardened animal fats and waxes, and waxes, lecithin, and one or more inorganic substances that are stable in neutral but soluble under acidic conditions. A feed additive composition for ruminants, which is coated with a coating composition containing the composition. 2. The composition according to claim 1, wherein lecithin is contained in an amount of 0.1 to 2 lecithin.
The ruminant feed additive composition according to claim 1, wherein the composition contains 0.1 to 10% by weight of 0% by weight and 0.1 to 10% by weight of an inorganic substance which is stable under neutral conditions but soluble under acidic conditions. 3. The inorganic substance which is stable under neutral conditions but soluble under acidic conditions is carbonate.
A feed additive composition for ruminants as described in the above. 4. The ruminant feed additive composition according to claim 1, wherein the inorganic substance which is stable under neutral conditions but soluble under acidic conditions is a calcium salt of pyrophosphate. 5. A feed containing the ruminant feed additive composition according to claim 1.