JPS63317053A - Feed additive for ruminants - Google Patents

Abstract

PURPOSE:To provide a feed additive for ruminants with excellent properties to pass biologically active substances through the rumen and release them in the organs after the maw, by coating cores containing biologically active substances with a specific coat. CONSTITUTION:A coating agent is prepared from lecithin and 12-24C straight- chained or branched saturated or unsaturated aliphatic monocarboxylic acid such as stearic acid, containing lecithin and glycerol fatty acid esters, hardened vegetable oil such as hardened castor oil, hardened animal oil such as hardened tallow oil and wax such as paraffin wax. Nuclei or cores containing biologically active substances such as lysine hydrochloride or Vitamin A are coated with the coating agent to give the subject additive for ruminants. Thus, the biologically active substances can be protected from the gastric juice in the rumens.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a feed additive for breeding animals, and more particularly, it protects biologically active substances from the forestomach fluid of breeding animals; The present invention relates to a feed additive for ruminants in which a biologically active substance is coated with a film of a protective substance so as to be released in the digestive tract after the abomasum.

The feed additive for ruminants of the present invention can be added to concentrate feed etc. and orally administered to ruminants such as cows and sheep, thereby allowing the biologically active substances to be effectively absorbed by the ruminants. .

[Conventional technology]

As a method for orally administering biologically active substances such as amino acids to expanded animals, biologically active substances are administered to protect the biologically active substances from fermentation and decomposition by microorganisms present in the rumen of ruminants. A method has been adopted in which a granulated preparation is administered by dispersing the protective substance in a matrix.

For example, granular preparations in which biologically active substances are dispersed in a matrix of fat, wax, etc.
It is disclosed in Japanese Patent No. 154956.

In addition, the present inventors also published a formulation in which a biologically active substance is dispersed in a matrix of a protective substance consisting of chitosan, an aliphatic monocarboxylic acid, and/or a hardened oil and fat, in Japanese Patent Laid-Open No. 175449/1983. I made a proposal and approved it. Furthermore, a leek preparation in which a core of granulated biologically active substances is coated with a film that is poorly soluble in neutral or alkaline water and easily soluble in acidic water was published in Japanese Patent Application Laid-Open No. 1983-1999. Disclosed.

[Problem that the invention seeks to solve]

Dispersion of biologically active substances in a matrix of protective substances,
In granulated preparations, biologically active substances are also distributed on the surface of the preparation, so if the ability to bypass the rumen of ruminants is important, the content of biologically active substances should be reduced to 40%.
It is necessary to keep it below. Furthermore, in preparations in which granules of biologically active substances are coated with a protective substance, it is possible to increase the content of biologically active substances. However, in the case of highly water-soluble biologically active substances, such as lysine hydrochloride, the ruminal bypass properties are greatly reduced in all of the above methods.

The present invention provides stable protection of biologically active substances, particularly water-soluble biologically active substances such as lysine hydrochloride, from the rumen juice of ruminants, and improves the ruminal bypass properties of biologically active substances. The object of the present invention is to provide a feed additive for expanded animals that has excellent release properties in the digestive tract after the abomasum.

[Means for solving problems]

As a result of intensive research to achieve the above object, the present inventors have found that a preparation in which a core mainly composed of lysine hydrochloride is coated with a film of a hydrophobic substance containing lecithin and glycerin fatty acid ester is The present invention was completed based on the discovery that it has excellent bypass properties and excellent release properties of biologically active substances in the digestive organs from the abomasum onwards.

The present invention provides a core containing a biologically active substance, and a linear or branched saturated or unsaturated aliphatic monomer having 12 to 24 carbon atoms containing lecithin and glycerin fatty acid ester coated on the core. This is an expanded animal feed additive characterized by comprising a film of at least one selected from the group consisting of carboxylic acid, hardened vegetable oil, hardened animal oil, and wax wax.

In the present invention, the biologically active substance is not particularly limited as long as it imparts physiological activity when administered to a rumen, but when administered orally, it is fermented and decomposed by microorganisms present in the rumen. Substances that are easily exposed to water are targeted, and substances with high water solubility are particularly preferred.

For example, amino acids such as methionine, lysine, tryptophan 1 threonine, glutamic acid, N-acylamino acids such as aspartic acid and their derivatives, vitamin A, vitamin A acetate, vitamin A palmitate, vitamin D2゜vitamin D! , vitamins such as vitamin B, vitamin E, and nicotinic acid, and their derivatives, and other veterinary drugs such as penicillin.

These may be used alone or in a mixture of two or more.

The core containing the biologically active substance contains the biologically active substance,
A solid obtained by molding a mixture of an excipient and a binder into a granular shape with a particle size of 0.4 to 2.5 mm, preferably a shape close to a spherical shape, from a mixture of an excipient and a binder and an optionally added inorganic substance. It is a thing.

There are no particular limitations on the method for producing the core containing the biologically active substance, and for example, it can be produced by pelletizing the mixture using an extrusion device and then spheronizing it using a spheronization device.

As a binder, cellulose derivatives such as methylcellulose, ethylcellulose, hydroxypropylcellulose, and hydroxypropylmethylcellulose; vinyl derivatives such as polyvinyl alcohol and polyvinylpyrrolidone;
Starches, fatty acids, hydrogenated vegetable oils, hydrogenated animal oils, etc. can be used.

Starch, microcrystalline cellulose, etc. are used as excipients.

In addition, calcium carbonate, calcium phosphate, talc, etc. are used as inorganic substances.

The film coated on the core containing the biologically active substance is composed of a linear or branched saturated or unsaturated aliphatic monocarboxylic acid having 12 to 24 carbon atoms, containing lecithin and glycerin fatty acid ester; It consists of at least one selected from the group consisting of hardened vegetable oil, hardened animal oil, and wax.

Lecithin extracted from egg yolk, soybean, etc. is preferably used, and does not necessarily have to be pure.

The glycerin fatty acid ester can be used without any particular restriction as long as it is an ester of glycerin and a saturated fatty acid or an unsaturated fatty acid, but glycerin monostearate, which is commercially available and can be made manually at low cost, is preferably used.

As an aliphatic monocarboxylic acid, myristic acid.

Palmitic acid, stearic acid, oleic acid, lauric acid, behenic acid, etc. can be used. Hydrogenated vegetable oils include hydrogenated castor oil, hydrogenated soybean oil, hydrogenated rapeseed oil, etc., hydrogenated animal oils include 54 hydrogenated beef tallow, extremely hardened beef tallow, etc., and waxes include beeswax, carnauba wax, whale wax, etc. Waxes such as paraffin wax, natural wax, and synthetic wax are used.

The composition of the film is in the range of 1 to 15 parts by weight of lecithin and 1 to 15 parts by weight of glycerin fatty acid ester based on 100 parts by weight of the base agent such as aliphatic monocarboxylic acid, hydrogenated vegetable oil, hydrogenated animal oil, or wax.

The feed additive of the present invention has a core containing the biologically active substance as a main component coated with the film.

Although it is preferable that the coating amount be small, it is required that at least the entire surface of the core is coated.

Usually, 5 to 30 parts by weight, preferably 10 to 30 parts by weight, is coated on 100 parts by weight of the core containing the biologically active substance as a main component.

There are no particular limitations on the coating method, and for example, a method of spray coating a melt or molten slurry of a coating material for film formation can be adopted.

[For production]

As described above, the present invention consists of a core mainly composed of a biologically active substance, and a film of a hydrophobic substance such as an aliphatic monocarboxylic acid containing lecithin and glycerin fatty acid ester coated on this core. This is an anti-separate animal feed additive characterized by the following.

In the present invention, the membrane coated on the core mainly composed of a biologically active substance protects the core mainly composed of a biologically active substance from the forestomach fluid of the ruminant, and allows the membrane to be transferred to the rumen. prevent the elution of biologically active substances.

As substances having these functions, there are used straight-chain or branched saturated or unsaturated aliphatic monocarboxylic acids having 12 to 24 carbon atoms, hydrogenated vegetable oils, hydrogenated animal oils, waxes and mixtures thereof.

In addition, the lecithin and glycerin fatty acid ester blended into this film can be used in the digestive organs of animals from the abomasum onwards.
Particularly in the small intestine, it emulsifies the aliphatic monocarboxylic acids, hydrogenated vegetable oils, hydrogenated animal oils, waxes, etc. and promotes the release of biologically active substances.

In the present invention, these act synergistically to provide excellent abomasal bypass properties and release properties of biologically active substances from the abomasum onwards in the digestive tract, resulting in biologically active substances being released from the abomasum and onward. Effectively absorbed by other animals.

〔Example〕

The present invention will be explained in more detail with reference to Examples and Comparative Examples.

However, the scope of the present invention is not limited to the same extent by the following examples.

+1) Preparation of animal feed additives (a) Production sample of biologically active substance-containing nuclear material L-1: Lysine-containing nuclear material lysine hydrochloride 1.900 g, calcium carbonate 300 g
and 800g of rapeseed hydrogenated oil in a nigu, 90g
After kneading for 1 hour while keeping the temperature at ℃, 3.9 m
Cylindrical granules were obtained using an extruder having a screen with openings of mφ.

The obtained granules were placed in a Henschel mixer, kept at 90°C, and while stirring, lysine hydrochloride/calcium carbonate =
220 g of mixed powder of 190/30 (weight ratio) was added in several portions and sprinkled on the growing granules to obtain spherical granules.

The obtained granules were sieved to 0.8 to 1.2 mm to obtain a lysine hydrochloride-containing core material (L-1).

Sample M-1: Methionine-containing nuclear substance 1.600 g of methionine, 200 g of calcium carbonate, and 200 g of microcrystalline cellulose were placed in a Nigu, and a binder solution in which 0.6 g of methylcellulose was dissolved in 69 g of water was added and kneaded. Cylindrical granules were obtained using an extruder having a screen with a diameter of 9 mm. Then, using a marmerizer, the obtained granules were granulated while adding a mixed powder of methionine/calcium carbonate = 80/20 (weight ratio) to obtain spherical granules. The obtained granules are 0.8 to 1.2 mm? , :,
Separation was performed to obtain a methionine-containing nuclear material (M-1).

(bl Coating of nuclear material (L-1) or (M-
100 g of 1) was placed in a fluidized bed, and a coating liquid prepared by dissolving a mixture prepared in an organic solvent to have the same coating composition as shown in Table 1 was spray coated to form a uniform coating on the surface of the core material.

The film composition is shown in Table 1.

(2) Evaluation test (a) Standard test 2 g of the preparation prepared in item +11 above was added to 200 CC of Mc Dougall's artificial gastric juice, which corresponds to the forestomach juice of cows.
After shaking and holding at a temperature of 37°C for 24 hours, it was taken out from Me Dougall's artificial gastric fluid and immersed in 200 cc of C1ark Lubs' pH 2 buffer solution corresponding to bovine abomasum fluid, and heated to a temperature of 37°C. Shake for an additional 4 hours. Furthermore, the preparation taken out from the C1ark Lubs buffer solution was immersed in 200 cc of a solution corresponding to bovine small intestine, and further shaken at a temperature of 37° C. for 4 hours.

In addition, taking into account the pH fluctuations of the forestomach fluid of the separated animals,
Stomach friendly Mc Dougall's artificial gastric juice has a pH of 5.2.
The formulation was treated under the same conditions as described above, except that it was prepared with acetic acid and used in place of Me Dougall's artificial gastric fluid.

Next, Mc Dougall's artificial gastric juice, C1ark
Lysine and methionine eluted in the Lubs buffer and small intestine corresponding fluid were quantified by ninhydrin colorimetric method or iodine titration method.

The test results are shown in Table 1 along with comparative examples.

Mc Dougall's artificial gastric juice: Sodium bicarbonate; 9.8 g, Potassium chloride; 0.5
7g, calcium chloride io, 04g, disodium phosphate dodecahydrate; 9.30g, sodium chloride; 0
．． 47 g and sodium sulfate heptahydrate: 0°12 g
C1ark, a solution of pH 8.3 dissolved in water;
Lubs buffer: 0.2N potassium chloride; 50mC0, 2N hydrochloric acid; 10,
6 m j! and water; 139.4 ml of mixed solution [Effects of the Invention] In the present invention, as shown in the above Examples (see Table 1), lecithin and glycerin fatty acid ester In preparations coated with a film that does not contain
It exhibits excellent bypass properties for gastric fluids, but exhibits almost no dissolution properties for abomasal fluids and small intestine fluids.

In addition, in preparations (see Examples) in which the core material containing the biologically active substance of the present invention is coated with a film containing lecithin and glycerin fatty acid ester, abomasum-compatible fluid and small intestine-compatible fluid It shows extremely excellent dissolution properties.

That is, when orally administered to an animal, the biologically active substance bypasses the abomasum, is eluted in the digestive organs from the abomasum onwards, and is effectively absorbed by the animal.

INDUSTRIAL APPLICABILITY The present invention provides a feed additive for separated animals that has excellent abomasal bypass properties and release properties of biologically active substances in the digestive organs from the abomasum onwards, and is useful in industry, particularly in livestock farming. is extremely significant.

Claims (1)

[Claims] (1) A linear or branched saturated or unsaturated aliphatic monocarboxylic acid having 12 to 24 carbon atoms containing a core containing a biologically active substance, lecithin coated on this core, and glycerin fatty acid ester A feed additive for ruminants, characterized by comprising at least one film selected from the group consisting of hardened vegetable oil, hardened animal oil, and wax.