JPS6128351A - Preparation of feed additive for ruminant - Google Patents

Abstract

PURPOSE:To obtain the titled additive having excellent disintegration property of the protecting substance in the abomasum and thereafter, and by-passing smoothly through the rumen, by melting a mixture of a water-soluble biologically active substance and a specific protecting substance, dripping the resultant molten slurry into hot water, and cooling and solidifying in chilled water. CONSTITUTION:(A) A water-soluble biologically active substance (e.g. water- soluble amino acid) is mixed with (B) a protecting substance insoluble in the gastric juice of the rumen of a ruminant and dissolving or disintegrating in the gastric juice of the abomasum, and if necessary with (C) a disintegrant, a seasoning, etc. The molten slurry of the mixture is dripped into hot water, and cooled and solidified in chilled water to obtain the objective granular additive. The component B is preferably the one composed of a 14-22C aliphatic monocarboxylic acid, a hardened animal or vegetable oil and an acid-soluble substance such as chitosan.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a method for producing a feed additive for ruminants.
More specifically, for ruminants, the biologically active substance is protected from decomposition by microorganisms living in the rumen of the ruminant, and is protected with a protective substance so that it can be digested and absorbed in the digestive tract from the abomasum onwards. This invention relates to a method for producing feed additives.

[Conventional technology]

Many microorganisms live in the rumen of ruminants such as cows and sheep, and when biologically active substances such as amino acids and proteins are orally administered, most of them are decomposed and inactivated by the microorganisms. It is known that it is not used effectively. Various feed additives for ruminants have been proposed in which these biologically active substances, especially essential amino acids, are protected from microorganisms in the rumen and protected with protective substances so that they can be digested and absorbed in the abomasum and beyond. Japanese Patent Publication No. 48-12785 discloses that biologically active substances are coated in granular form with a continuous film of one or more protective substances such as triglycerides, animal or vegetable fats obtained by hydrogenating triglycerides, and bran wax. A feed additive is disclosed in which a core material obtained by granulating a mixture of these protective substances and a biologically active substance is coated with one type of protective substance. .

Special Publication No. 56-1057, Special Publication No. 59-10780
Various protective substances have been proposed, and granular feed additives prepared by granulating a mixture of a protective substance and a biologically active substance are described in Japanese Patent Application Laid-open No. 58-175449 and the like.

[Problem that the invention seeks to solve]

In the feed additive disclosed in Japanese Patent Publication No. 48-12785, the rumen bypass property of ruminants is good due to the strong coating of the protective substance, especially the continuous film coated with the granules. The disintegration of protective substances in the digestive tract after the abomasum is poor, and biologically active substances are excreted without being effectively absorbed. In addition, in the publication,
Feed additives are manufactured through a two-step process of granulation and coating. Feed additives other than those disclosed in Japanese Patent Publication No. 48-12785 propose protective substances with improved disintegration properties in the digestive tract after the abomasum, and Japanese Patent Publication No. 58-175449 proposes
This was proposed by the applicant.

These granules are simply granulated mixtures of a protective substance and a biologically active substance, so the surface of the granules contains biologically active substances that are not coated with a protective substance. When exposed, if the biologically active substance is water-soluble, it will leach out in the rumen of the ruminant, causing the granules to disintegrate in the rumen, causing the granules to disintegrate. The ruminal bypass property of

The present invention uses a protective substance that has improved disintegration in the digestive tract after the abomasum, and has no biologically active substance exposed on its surface. The object is to provide an improved method for producing a granular feed additive with improved gastric bypass properties.

[Means for solving problems]

The present invention provides granular ruminant preparations in which a molten slurry of a mixture of water-soluble biologically active substances, protective substances and optionally added additives is dropped into hot water and then introduced into cold water to cool and solidify. This is a method for producing feed additives.

In the present invention, a water-soluble biologically active substance is a substance that exhibits biological activity when administered to ruminants,
In addition, since they are water-soluble, they are degraded in the rumen when administered orally and are difficult to digest and absorb effectively, such as water-soluble amino acids and their derivatives such as methionine and lysine hydrochloride, nicotinic acid, nicotinamide, These include water-soluble vitamins such as vitamin H, sugars such as glucose and fructose, and water-soluble veterinary medicines. The protective substance may be any substance as long as it is insoluble in the ruminal fluid of ruminants and is disintegrated or digested and absorbed in the digestive tract from the abomasum onwards, and any of the substances described in the above-cited publications may be used. Can be used. Preferably, those mainly composed of animal and vegetable oils and fats and waxes having a melting point of 45 to 100°C, and more preferably one of aliphatic monocarboxylic acids having 14 to 22 carbon atoms, hardened animal oils, and hardened vegetable oils. or JP-A-58-17544 consisting of two or more species and an acid-soluble substance such as chitosan or acid-soluble polymer.
Protective substances described in Publication No. 9 etc. are used.

Additives added as desired include disintegration aids such as acid-soluble inorganic salts that help disintegrate protective substances in the digestive tract from the abomasum onward, and density adjustment agents made of inorganic substances to adjust the density of feed additives. agents, flavoring agents, coloring agents, etc.

In the present invention, a required amount of optional additives is added to a mixture of 100 parts by weight of the biologically active substance and 50 to 500 parts by weight of the protective substance. Wax, fatty acids, etc. are heated to a temperature higher than their melting temperature to prepare a molten slurry. This molten slurry is made into droplets using a nozzle, a rotating disk, etc.
Drop into warm water. The hot water need not be pure water but may be a dilute solution of a water-soluble substance, the temperature of which varies depending on the solubility of the water-soluble biologically active substance, but between 35 and 8
It is 0°C. Water-soluble biologically active substances dissolved in hot water can be recrystallized by evaporation concentration or cooling, or
It can be recovered by solvent extraction etc. Next, the slurry is introduced into cold water and solidified by cooling to obtain the desired granular feed additive. The cold water also does not need to be pure water, and its temperature may be as long as the temperature after introducing the hot water slurry is below the melting point of the protective substance, and the temperature is between 0 and 30.
A range of 0.degree. C. is preferably used. The solidified feed additive is filtered and dried using conventional methods to produce a product.

[For production]

As a result of intensive research aimed at improving the disintegration resistance of feed additives for ruminants in the rumen and improving their bypass properties, the present inventors discovered that water-soluble biologically active substances, protective substances, and optionally added By dropping a molten slurry of a mixture of additives into hot water, the droplets of the molten slurry coat the mixture with a coating of molten material under the action of the surface tension of the water-insoluble molten material. A nearly perfectly spherical feed additive for ruminants in which a water-soluble substance is exposed on the surface and is then covered with a protective coating by introducing the spherical body into cold water and cooling and solidifying it. The present invention was completed based on the discovery that the following can be obtained.

That is, dropping the molten slurry into warm water makes the dosage form of the ruminant feed additive almost spherical, and at the same time forms a protective substance coating on its surface free of water-soluble substances.

〔Example〕

Below, the present invention will be explained in more detail with reference to Examples and Test Examples. However, the scope of the present invention is not limited in any way by the following examples. Example 1 52 parts by weight of 54" hardened oil (manufactured by NOF) was melted by heating to 75°C, and while stirring, methionine di35 parts by weight, chitosan: 3 parts by weight, calcium carbonate: 8 parts by weight, and monobasic calcium phosphate; 2 parts by weight were added to prepare a molten slurry.The prepared molten slurry was heated through a nozzle at 40°C.
The mixture was dropped into hot water at 20° C., and then cooled and solidified by introducing into cold water at 20° C. to obtain spherical particles having an average diameter of 3.5 rtan.

The surfaces of the obtained particles were coated with a fusible substance, and no non-fusible substance was observed to be exposed.

Examples 2 to 5 In Example 1, the components and composition ratio of the molten slurry were changed, and the treatment was performed under the same conditions as in Example 1.
True spherical particles similar to those were obtained.

The components and composition ratio of the molten slurry are shown in Table 1 together with Example 1.

Comparative Examples 1 to 5 The molten slurry prepared in Examples 1 to 5 was dropped onto a stainless steel plate cooled to 4°C using the same nozzle as used in the example, cooled and solidified to form a slurry with an average diameter of 4 m. Hemispherical particles with a top height were obtained.

Non-melting substances were present in spots on the surfaces of the obtained particles.

The components and composition ratio of the molten slurry are shown in Table 1.

Test Examples The particles 2f obtained in the Examples and Comparative Examples were mixed with Me, Dougall's artificial saliva 2, which corresponds to the gastric juice of the rumen of a cow.
00ml and kept at a temperature of 39°C for 24 hours while being constantly moved by a vibrating device. Then,
C1ark-L, which corresponds to the gastric juice of the abomasum of cattle,
It was immersed in 200 m of PH2 buffer solution (UBS) and heated at a temperature of 39°C for 4 hours with constant movement using a vibration device.
Holds time.

After the immersion test was completed, the biologically active substance concentration in the artificial saliva and buffer solution was measured, and the elution rate of the biologically active substance was calculated.

The elution rate of biologically active substances into artificial saliva and buffer solution is shown in Table 1.

The Me, Dougall's artificial saliva and C1ark-Lub+s buffer used in this test were as follows.

(Mc, Dougal's artificial saliva) Sodium bicarbonate: 9.8? , potassium chloride 20,57f, calcium chloride: 0.04t, sodium phosphate dodecahydrate; 9°30f1 sodium chloride: 0.47f and magnesium sulfate heptahydrate: 0°129, pure water: 1t (C1ark-Lubs buffer) 0.2N potassium chloride aqueous solution: 50ml and 0.2N
An aqueous solution of PH2 in which 10.6ml of hydrochloric acid is dissolved in 139.41d of pure water. [Effects of the Invention] The spherical feed additive for ruminants produced by the method of the present invention has the elution rate shown in Table 1 above. As is clear from the test results, compared to the conventionally produced ruminant feed additive (comparative example), Mc, which corresponds to the gastric juice of the cow's rumen,
Although the elution rate of biologically active substances in the artificial saliva of Douga 11 is as low as below the plate, the elution rate of biologically active substances in the buffer solution of C1ark-Lubs, which corresponds to the gastric juice of the fourth helmet, is Indicates a value equivalent to y. In other words, this shows that the ability to bypass the rumen of ruminants is significantly improved.

In addition, the feed additive for ruminants produced by the method of the present invention has a true spherical shape and the surface is coated with a protective substance, so when it is orally administered to ruminants, the ruminant remains in the rumen. It is expected that abrasion and disintegration caused by mixing can be prevented, and the effective utilization rate of biologically active substances is higher than that of ruminant feed additives produced by conventional methods.
It can be expected that the difference will be greater than the difference in the elution rate of 1 ml to artificial saliva.

Furthermore, the method of the present invention requires a simple, special coating process that allows the production of ruminant feed additives of any particle size by selecting the molten slurry dropletization device and its operating conditions. This is an industrially superior method that does not require

The present invention provides an industrial method for producing a feed additive for ruminants that improves the ruminant properties of ruminants, and has extremely great industrial significance.

Claims (5)

[Claims] (1) Melting of a mixture consisting of a water-soluble biologically active substance, a protective substance that is insoluble in the ruminal juice of a ruminant and soluble or disintegrating in the abomasal juice, and optionally added additives. A method for producing a feed additive for ruminants, which comprises dropping the slurry into hot water, then introducing it into cold water, cooling it, and solidifying and granulating it. (2) Claim (1) wherein the water-soluble biologically active substance is one or more of water-soluble amino acids and their derivatives, water-soluble vitamins, sugars, and water-soluble veterinary drugs. Method for producing the described feed additive for ruminants (3) The protective substance is one or two of linear or branched saturated or unsaturated aliphatic monocarboxylic acids having 14 to 22 carbon atoms, hydrogenated animal oils, and hydrogenated vegetable oils.
Claim 1 (1) consisting of at least one species and an acid-soluble substance
Method for producing feed additives for ruminant animals described in section ) (4) A method for producing a feed additive for ruminants according to claim (1), wherein the hot water temperature is 35 to 80°C. (5) The chilled water temperature is 0 to 30°C (
Method for manufacturing the feed additive for ruminant animals described in section 1)