JPH0356500A - Extraction of egg yolk phosvitin - Google Patents
Extraction of egg yolk phosvitinInfo
- Publication number
- JPH0356500A JPH0356500A JP1191291A JP19129189A JPH0356500A JP H0356500 A JPH0356500 A JP H0356500A JP 1191291 A JP1191291 A JP 1191291A JP 19129189 A JP19129189 A JP 19129189A JP H0356500 A JPH0356500 A JP H0356500A
- Authority
- JP
- Japan
- Prior art keywords
- egg yolk
- phosvitin
- aqueous solution
- ammonium sulfate
- minutes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108010000912 Egg Proteins Proteins 0.000 title claims abstract description 69
- 102000002322 Egg Proteins Human genes 0.000 title claims abstract description 69
- 210000002969 egg yolk Anatomy 0.000 title claims abstract description 66
- 235000013345 egg yolk Nutrition 0.000 title claims abstract description 66
- 102100027992 Casein kinase II subunit beta Human genes 0.000 title claims abstract description 49
- 101710158100 Casein kinase II subunit beta Proteins 0.000 title claims abstract description 49
- 238000000605 extraction Methods 0.000 title description 3
- 239000007864 aqueous solution Substances 0.000 claims abstract description 29
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 11
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 9
- 239000000203 mixture Substances 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 229920006395 saturated elastomer Polymers 0.000 abstract description 3
- 239000003963 antioxidant agent Substances 0.000 abstract description 2
- 230000003078 antioxidant effect Effects 0.000 abstract description 2
- 238000001556 precipitation Methods 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract 3
- 239000002994 raw material Substances 0.000 description 11
- 239000000843 powder Substances 0.000 description 10
- 238000005119 centrifugation Methods 0.000 description 8
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 5
- 239000002244 precipitate Substances 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000000706 filtrate Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 229910001431 copper ion Inorganic materials 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 102000007982 Phosphoproteins Human genes 0.000 description 1
- 108010089430 Phosphoproteins Proteins 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 238000005571 anion exchange chromatography Methods 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 108010015053 lipovitellin Proteins 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000000464 low-speed centrifugation Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000031787 nutrient reservoir activity Effects 0.000 description 1
- 239000004584 polyacrylic acid Substances 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は、卵黄ホスビチンの抽出方法に関する．〔従来
の技術〕
卵黄ホスビチンは卵黄に含まれる蛋白質のうちの約２％
を占めるリン蛋白質であり、鉄や銅イオンをキレートす
る作用が強く、天然の抗酸化剤となる。また、カルシウ
ムイオンとも結合しやすいので、カルシウム分強化剤の
補助剤として期待されている。DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Application) The present invention relates to a method for extracting egg yolk phosvitin. [Prior art] Egg yolk phosvitin accounts for approximately 2% of the protein contained in egg yolk.
It is a phosphoprotein that makes up the majority of iron and copper ions, and has a strong effect of chelating iron and copper ions, making it a natural antioxidant. In addition, since it easily binds to calcium ions, it is expected to be used as an adjunct to calcium content enhancers.
しかしながら、従幻ト黄からホスビチンを抽出するには
、Ｊ． Ａｇｒｉｃ． Ｆｏｏｄ Ｃｈｅｗ．， Ｖｏ
ｌ ２５ ．Ｎａ３１９７７． ６３２〜６３７頁に
みられるように、希食塩水中に生卵黄を加えて遠心分離
し、ホスビチンとりボビテリンの混合物からなる沈殿（
凝集物）を得、この凝集物をＩＮの食塩水に溶解させた
後、この食塩水に高濃度の硫安水溶液を加えてから数万
回転／分（ｒ’ｐ■）遠心分離して、沈殿（リポビテリ
ン）を除き、得られた上澄よりホスビチンを採取する方
法がとられていた．
このように、従来法によるとホスビチンの抽出工程が複
雑であるため、得ら．れたホスビチンは高価なものとな
ってしまうという問題があった．また、従来法では、ホ
スビチンの原料として生卵黄しか使用できず、したがっ
て清水に不溶な脱脂卵黄は原料として使用できないとい
う問題があった．〔発明が解決しようとする課題〕
ところで、ホスビチンの需要が見込まれる今日において
は、ホスビチンを低コストで供給することが急務であり
、卵黄や脱脂卵黄粉からホスビチンを工業的に抽出する
技術の確立が望まれている。However, in order to extract phosvitin from Fugentohuang, J. Agric. Food Chew. , Vo
l 25. Na31977. As shown on pages 632-637, raw egg yolk is added to dilute saline solution and centrifuged to form a precipitate consisting of a mixture of phosvitin and bovitellin.
After dissolving this aggregate in IN saline solution, a highly concentrated aqueous ammonium sulfate solution was added to the saline solution, and centrifugation was performed at tens of thousands of revolutions per minute (r'p■) to precipitate. (Lipovitellin) was removed, and phosvitin was collected from the resulting supernatant. As described above, according to the conventional method, the extraction process of phosvitin is complicated, so it is difficult to obtain phosvitin. The problem was that the phosvitin obtained was expensive. Furthermore, in the conventional method, only raw egg yolk can be used as a raw material for phosvitin, and therefore defatted egg yolk, which is insoluble in fresh water, cannot be used as a raw material. [Problem to be solved by the invention] Nowadays, when demand for phosvitin is expected, there is an urgent need to supply phosvitin at a low cost, and it is necessary to establish a technology for industrially extracting phosvitin from egg yolk or defatted egg yolk powder. is desired.
そこで本発明者等は、そのような技術を開発すべく研究
を重ねた結果、本発明を完威したものである．
〔課題を解決するための手段〕
本発明は、卵黄ホスビチンの抽出法に関し、卵黄又は脱
脂卵黄に硫安水溶液を加えて撹拌し、この水溶液中にホ
スビチンを溶出させた後、水溶液から凝集物を除き、次
に、この水溶液からホスビチンを採取することを特徴と
するものである。Therefore, the present inventors conducted repeated research to develop such a technology, and as a result, they have perfected the present invention. [Means for Solving the Problems] The present invention relates to a method for extracting egg yolk phosvitin, which involves adding an ammonium sulfate aqueous solution to egg yolk or defatted egg yolk and stirring, eluting phosvitin into this aqueous solution, and then removing aggregates from the aqueous solution. Next, phosvitin is collected from this aqueous solution.
本発明において卵黄とは、殻付卵を割卵して卵白を除い
て得られる生卵黄のほか、生卵黄を凍結エタノールやヘ
キサン等の溶媒にて卵黄中に含まれるレシチン・中性脂
質等の油脂威分の一部または全部を取り除いたものをい
い、通常、乾燥状に仕上げて、脱脂卵黄粉として市販さ
れている．脱脂卵黄は溶媒処理によって卵黄蛋白質が変
性しており、清水に不溶となっているため、従来、家畜
や稚魚の飼料にしか用途がなかったものである．本発明
の実施に当って、まず原料の卵黄又は脱脂卵黄を用意す
る。卵黄が冷凍卵黄の場合、予め解凍しておくとよい。In the present invention, egg yolk refers to raw egg yolk obtained by breaking shell eggs and removing the egg white, as well as raw egg yolk obtained by freezing the raw egg yolk in a solvent such as frozen ethanol or hexane to remove lecithin, neutral lipids, etc. contained in the egg yolk. Egg yolk powder is a product from which some or all of the oil and fat content has been removed, and is usually dried and sold as defatted egg yolk powder. In defatted egg yolk, the yolk protein has been denatured by solvent treatment, making it insoluble in fresh water, so it has traditionally only been used as feed for livestock and young fish. In carrying out the present invention, first, egg yolk or defatted egg yolk as a raw material is prepared. If the egg yolk is frozen, it is best to thaw it beforehand.
また卵黄が卵黄粉の場合又は脱脂卵黄が乾燥状の場合に
はそのまま用いればよい．
次に、この原料に硫安水溶液を加えて撹拌し、この水溶
液中に原料に含まれているホスビチンを溶出させる．ホ
スビチンの溶出を効率よく行なうには、乾燥卵黄や脱脂
卵黄を用いる場合には、７０％飽和硫安水溶液を加え、
５０〜３００ｒｐｍで３〜２４時間撹拌する事が望まし
い．また生卵黄を用いる場合には、最終硫安濃度が７０
％飽和になるように１００％飽和硫安水溶液を加えた後
この水溶液を８０”Ｃ−１００℃で１５分程加熱し、卵
黄蛋白質を凝固させるのが望ましい。If the egg yolk is powdered egg yolk or dried defatted egg yolk, it can be used as is. Next, an aqueous ammonium sulfate solution is added to this raw material and stirred to elute phosvitin contained in the raw material into this aqueous solution. In order to elute phosvitin efficiently, when using dried or defatted egg yolk, add a 70% saturated aqueous ammonium sulfate solution.
It is desirable to stir at 50 to 300 rpm for 3 to 24 hours. In addition, when using raw egg yolk, the final ammonium sulfate concentration is 70
It is desirable to add a 100% saturated ammonium sulfate aqueous solution to achieve % saturation and then heat this aqueous solution at 80"C-100C for about 15 minutes to coagulate the egg yolk protein.
次に、ホスビチンを溶出した硫安水溶液から硫安水溶液
に不溶な凝集物を除く．凝集物を除くには、低速遠心分
離法や濾過法を採用すればよい。Next, remove aggregates that are insoluble in the ammonium sulfate aqueous solution from which phosvitin has been eluted. To remove aggregates, low-speed centrifugation or filtration may be employed.
最後に、上記凝集物を除いた水溶液からホスビチンを採
取する．ホスビチンを採取するには、例えば、水溶液を
ｐｉ １． ５〜１．８に調整してホスビチンを沈殿さ
せ、これを水溶液から分離する方法や、水溶液を希塩酸
に対して透析してホスビチンを沈殿させ、これを水溶液
から分離する方法などがある．
以上のように本発明の卵黄ホスビチンの抽出方法は、撹
拌、沈殿及び分離工程の組み合せからなるものであり、
大型タンクや遠心分離機を用いて工業的に行なうことが
でき、卵黄ホスビチン功大量生産が可能である．そして
、このようにして得られた卵黄ホスビチンは極めて簡便
な工程で抽出されるにも拘らず、純度の高い（９５％以
上）ものである．
〔実施例〕
皇嵐班上
脱脂卵黄粉（キューピー■製、商品名「ヨークプロテイ
ンＨＪ）１００ｇに７０％飽和硫安水溶液２ｌを加え、
２ ０ ０ ｒｐｍで２４時間撹拌した．次に、この水
溶液を３． ０ ０ ０ｒｐｔａで１５分間遠心分離し
て凝集物を除いた後、Ｎｌ１５Ｃ濾紙で濾過した．
次に、得られた濾液を透析チューブ（カット分子量約１
０．０００）に詰め、約２０倍量の０．０４Ｎ塩酸に対
し５〜６℃で２４時間透析した後、３． ，０ ０ ０
ｒｐｍで１５分間遠心分離し、得られた沈殿物を０．
０ ４ Ｎの塩酸で洗浄し、さらに蒸留水に対して透
析後凍結乾燥して卵黄ホスビチン７４０■を得た．
裏簾量主
脱脂卵黄粉（実施例１で用いたものと同じ）１００ｇに
７０％飽和硫安水溶液２ｌを加え、２００ｒｐｍで２４
時間撹拌シタ．
次に、この水溶液を３．ＯＯＯｒｐ−で１５分間遠心分
離して凝集物を除いた後、Ｎｔ１５Ｃ濾紙で濾過した．
そして、濾液のｐＨを１．８に調整してホスビチンを凝
集させた後、３．０００ｒｐ−で１５分間遠心分離して
沈殿物を回収し、この沈殿物を蒸留水に対して透析後、
凍結乾燥したところ、卵黄ホスビチン６６４■を得た．
実１０東よ
原料として卵黄粉（キューピー■製、商品名「卵黄粉」
）ｌＯｇを用い、実施例１と同じ方法でこの卵黄粉を処
理して卵黄ホスビチン１０６■を得た．
裏益班工
原料として卵黄粉（実施例３で用いたものと同じ）Ｌｏ
ｇを用い、実施例２と同じ方法でこの卵黄粉を処理して
卵黄ホスビチン３０■を得た．裏旌斑ｉ
原料として殻付卵を割卵して得た生卵黄２０ｇを用い、
これに蒸留水４０ａ＋ｆ飽和硫安水溶液１０ｍｌを加え
、ｔ ｏ ｏ ’ｃで１５分間加熱した。Finally, collect phosvitin from the aqueous solution from which the aggregates have been removed. To collect phosvitin, for example, an aqueous solution is prepared by pi 1. There is a method in which phosvitin is precipitated by adjusting it to 5 to 1.8 and separated from the aqueous solution, and a method in which the aqueous solution is dialyzed against dilute hydrochloric acid to precipitate phosvitin and this is separated from the aqueous solution. As described above, the egg yolk phosvitin extraction method of the present invention consists of a combination of stirring, precipitation, and separation steps,
It can be carried out industrially using large tanks and centrifuges, making it possible to mass-produce egg yolk phosvitin. Although the egg yolk phosvitin thus obtained is extracted by an extremely simple process, it has a high purity (95% or more). [Example] 2 liters of 70% saturated ammonium sulfate aqueous solution was added to 100 g of Koranban upper defatted egg yolk powder (manufactured by Kewpie ■, trade name "Yoke Protein HJ"),
The mixture was stirred at 200 rpm for 24 hours. Next, add this aqueous solution to 3. After centrifugation at 0 0 0 rpm for 15 minutes to remove aggregates, the mixture was filtered through Nl15C filter paper. Next, the obtained filtrate was poured into a dialysis tube (cut with a molecular weight of about 1
0.000) and dialyzed against approximately 20 times the amount of 0.04N hydrochloric acid at 5 to 6°C for 24 hours. ,0 0 0
Centrifugation for 15 minutes at rpm, and the resulting precipitate was centrifuged at 0.05 rpm.
The product was washed with 0 4 N hydrochloric acid, further dialyzed against distilled water, and then freeze-dried to obtain 740 ml of egg yolk phosvitin. Add 2 liters of 70% saturated ammonium sulfate aqueous solution to 100 g of main defatted egg yolk powder (same as that used in Example 1), and mix at 200 rpm for 24 hours.
Stir for a while. Next, add this aqueous solution to 3. After centrifugation at OOOrp- for 15 minutes to remove aggregates, the mixture was filtered through Nt15C filter paper.
After adjusting the pH of the filtrate to 1.8 to aggregate phosvitin, the precipitate was collected by centrifugation at 3.000 rpm for 15 minutes, and this precipitate was dialyzed against distilled water.
After freeze-drying, 664 μ of egg yolk phosvitin was obtained. The raw material is egg yolk powder (manufactured by Kewpie ■, product name: "Egg Yolk Powder")
) This egg yolk powder was treated in the same manner as in Example 1 using 10g of egg yolk phosvitin. Egg yolk powder (same as that used in Example 3) Lo as a raw material
This egg yolk powder was treated in the same manner as in Example 2 using 30 μg of egg yolk phosvitin. Ura-kei-ma-i Using 20g of raw egg yolk obtained by cracking shelled eggs as the raw material,
To this was added 10 ml of distilled water 40a+f saturated aqueous ammonium sulfate solution, and the mixture was heated for 15 minutes at t o o' c.
次に３． ０ ０ ０ｒｐ＋ｓで１５分間遠心分離して
浮上した凝集物を除いて、下層液を得た．この下層液を
Ｎｔ１５Ｃの濾紙で濾過し、濾液を実施例１と同様に処
理したところ、卵黄ホスビチン９３■を得た．裏旌銖ｉ
原料として、生卵黄（実施例５で用いたものと同じ）２
０ｇを用い、実施例５と同じ方法で下層液を得た。この
下層液を実施例２と同じ方法で処理したところ、卵黄ホ
スビチン２７■を得た。Next 3. After centrifugation at 000 rpm+s for 15 minutes, floating aggregates were removed to obtain a lower layer liquid. This lower layer liquid was filtered through a Nt15C filter paper, and the filtrate was treated in the same manner as in Example 1 to obtain 93 ml of egg yolk phosvitin. As a raw material, raw egg yolk (same as that used in Example 5) 2
A lower layer liquid was obtained in the same manner as in Example 5 using 0 g. When this lower layer liquid was treated in the same manner as in Example 2, 27 ml of egg yolk phosvitin was obtained.
実施例ｌ乃至実施例６で得られた卵黄ホスビチンをテス
ト区とし、また、生卵黄８３ｇに等量の０． １ ６
Ｎ食塩水を加え、２０． ０００ｒｐｍで３０分間遠心
分離し、得られた沈殿物を１６０ｇの０． １ ６ Ｎ
食塩水中に分散させ、次にこの懸濁液を１０．００Ｏｒ
ｐｍで３０分間遠心分離して沈殿物を得、この沈殿物を
９５ｇのＩＮ食塩水中に溶解させた後、この溶液に２２
６ｇの１００％飽和硫安水溶液を加え、而る後、この水
溶液を２０．０００ｒｐ＋ｍで１５分間遠心分離して濾
液を得、この濾液から実施例１と同じ方法でホスビチン
を採取し（公知のＪ． ＡｇｒｉｃＦｏｏｄ ＣｈｅＩ
１に準ずる方法）、得られた卵黄ホスビチンを対照区と
した。The egg yolk phosvitin obtained in Examples 1 to 6 was used as the test group, and 83 g of raw egg yolk was given an equivalent amount of 0. 1 6
Add N salt solution, 20. Centrifugation was performed at 0.000 rpm for 30 minutes, and the resulting precipitate was separated into 160 g of 0.000 rpm. 1 6 N
Dispersed in saline solution, then this suspension was heated to 10.00 Or
Centrifugation for 30 minutes at
After adding 6 g of 100% saturated ammonium sulfate aqueous solution, this aqueous solution was centrifuged at 20.000 rpm+m for 15 minutes to obtain a filtrate, from which phosvitin was collected in the same manner as in Example 1 (known in J. AgricFoodCheI
1), the obtained egg yolk phosvitin was used as a control.
そして、上記各卵黄ホスビチンの収率を算出すると共に
、その純度を測定したところ、表−１の結果が得られた
．
尚、収率は原料中に含まれる卵黄ホスビチン含量の理論
値に対する得られた卵黄ホスビチンの百分率（重量比）
である．また、純度は、ＳＤＳボリアクリルア逅ドゲル
電気泳動の結果と高速液体クロマトグラフィ（陰イオン
交換クロマト使用）の結果より測定した．
表−１
〔発明の効果〕
以上述べたように、本発明によれば、簡便な方法により
純度の高い卵黄ホスビチンを収率よく得ることができる
．そして、卵黄ホスビチンを大型タンクや遠心分離機を
用いて大量生産ができるので、卵黄ホスビチンを安価に
提供することが可能である。また、原料としてこれまで
あまり用途がなかった脱脂卵黄を用いて卵黄ホスビチン
を得ることができるという利点も有する。Then, when the yield of each egg yolk phosvitin was calculated and its purity was measured, the results shown in Table 1 were obtained. In addition, the yield is the percentage (weight ratio) of the obtained egg yolk phosvitin to the theoretical value of the egg yolk phosvitin content contained in the raw material.
It is. Purity was determined based on the results of SDS polyacrylic acid gel electrophoresis and high performance liquid chromatography (using anion exchange chromatography). Table 1 [Effects of the Invention] As described above, according to the present invention, highly pure egg yolk phosvitin can be obtained in good yield by a simple method. Since egg yolk phosvitin can be mass-produced using large tanks and centrifuges, it is possible to provide egg yolk phosvitin at a low cost. It also has the advantage that egg yolk phosvitin can be obtained using defatted egg yolk, which has not had much use as a raw material so far.
Claims (1)
溶液中にホスビチンを溶出させた後水溶液から凝集物を
除き、次に、この水溶液からホスビチンを採取すること
を特徴とする卵黄ホスビチンの抽出方法。A method for extracting egg yolk phosvitin, which comprises adding an aqueous ammonium sulfate solution to egg yolk or defatted egg yolk and stirring the mixture, eluting phosvitin into the aqueous solution, removing aggregates from the aqueous solution, and then collecting phosvitin from the aqueous solution. .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1191291A JP2664999B2 (en) | 1989-07-26 | 1989-07-26 | Egg yolk phosvitin extraction method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1191291A JP2664999B2 (en) | 1989-07-26 | 1989-07-26 | Egg yolk phosvitin extraction method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0356500A true JPH0356500A (en) | 1991-03-12 |
| JP2664999B2 JP2664999B2 (en) | 1997-10-22 |
Family
ID=16272124
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1191291A Expired - Lifetime JP2664999B2 (en) | 1989-07-26 | 1989-07-26 | Egg yolk phosvitin extraction method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2664999B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002256000A (en) * | 2001-02-28 | 2002-09-11 | Transgenic Inc | Antibody for specifically recognizing vitellogenin of bird and vitellogenin assay system of bird |
-
1989
- 1989-07-26 JP JP1191291A patent/JP2664999B2/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002256000A (en) * | 2001-02-28 | 2002-09-11 | Transgenic Inc | Antibody for specifically recognizing vitellogenin of bird and vitellogenin assay system of bird |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2664999B2 (en) | 1997-10-22 |
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