JP2664999B2 - Egg yolk phosvitin extraction method - Google Patents
Egg yolk phosvitin extraction methodInfo
- Publication number
- JP2664999B2 JP2664999B2 JP1191291A JP19129189A JP2664999B2 JP 2664999 B2 JP2664999 B2 JP 2664999B2 JP 1191291 A JP1191291 A JP 1191291A JP 19129189 A JP19129189 A JP 19129189A JP 2664999 B2 JP2664999 B2 JP 2664999B2
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- phosvitin
- yolk
- egg yolk
- aqueous solution
- egg
- Prior art date
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Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、卵黄ホスビチンの抽出方法に関する。Description: TECHNICAL FIELD The present invention relates to a method for extracting yolk phosvitin.
卵黄ホスビチンは卵黄に含まれる蛋白質のうちの約2
%を占めるリン蛋白質であり、鉄や銅イオンをキレート
する作用が強く、天然の抗酸化剤となる。また、カルシ
ウムイオンとも結合しやすいので、カルシウム分強化剤
の補助剤として期待されている。Egg yolk phosvitin is about 2 of the proteins contained in egg yolk.
% Of the protein, which has a strong ability to chelate iron and copper ions and is a natural antioxidant. Further, since it easily binds to calcium ions, it is expected as an auxiliary agent for a calcium content enhancer.
しかしながら、従来卵黄からホスビチンを抽出するに
は、J.Agric.Food Chem.,Vol25,No.3 1977,632〜637頁
にみられるように、希食塩水中に生卵黄を加えて遠心分
離し、ホスビチンとリポビテリンの混合物からなる沈殿
(凝集物)を得、この凝集物を1Nの食塩水に溶解させた
後、この食塩水に高濃度の硫安水溶液を加えてから数万
回転/分(rpm)遠心分離して、沈殿(リポビテリン)
を除き、得られた上澄よりホスビチンを採取する方法が
とられていた。However, conventionally, to extract phosvitin from egg yolk, as shown in J. Agric. Food Chem., Vol 25, No. 3 1977, 632-637, add fresh egg yolk in diluted saline and centrifuge. A precipitate (aggregate) consisting of a mixture of phosvitin and lipovitelin is obtained, and the aggregate is dissolved in 1N saline, and then a high-concentration aqueous solution of ammonium sulfate is added to the saline, and then tens of thousands of revolutions / minute (rpm) Centrifuge and precipitate (lipovitelin)
Except that phosvitin was collected from the resulting supernatant.
このように、従来法によるとホスビチンの抽出工程が
複雑であるため、得られたホスビチンは高価なものとな
ってしまうという問題があった。また、従来法では、ホ
スビチンの原料として生卵黄しか使用できず、したがっ
て清水に不溶な脱脂卵黄は原料として使用できないとい
う問題があった。As described above, according to the conventional method, there is a problem that the obtained phosvitin is expensive because the extraction step of phosvitin is complicated. Further, the conventional method has a problem that only raw egg yolk can be used as a raw material of phosvitin, and therefore, defatted egg yolk insoluble in fresh water cannot be used as a raw material.
〔発明が解決しようとする課題〕 ところで、ホスビチンの需要が見込まれる今日におい
ては、ホスビチンを低コストで供給することが急務であ
り、卵黄や脱脂卵黄からホスビチンを工業的に抽出する
技術の確立が望まれている。[Problems to be Solved by the Invention] By the way, today, when demand for phosvitin is expected, it is urgently necessary to supply phosvitin at low cost. Is desired.
そこで本発明者等は、そのような技術を開発すべく研
究を重ねた結果、本発明を完成したものである。Thus, the present inventors have conducted research for developing such a technique, and as a result, have completed the present invention.
本発明は、卵黄ホスビチンの抽出法に関し、卵黄又は
脱脂卵黄に硫安水溶液を加えて撹拌し、この水溶液中に
ホスビチンを溶出させた後、水溶液から凝集物を除き、
次に、この水溶液からホスビチンを採取することを特徴
とするものである。The present invention relates to a method for extracting yolk phosvitin, adding an aqueous solution of ammonium sulfate to yolk or defatted egg yolk and stirring, elutes phosvitin into this aqueous solution, removes aggregates from the aqueous solution,
Next, phosvitin is collected from the aqueous solution.
本発明において卵黄とは、殻付卵を割卵して卵白を除
いて得られる生卵黄のほか、生卵黄を凍結して得られる
冷凍卵黄、生卵黄を乾燥して得られる卵黄粉等をいう。
また、脱脂卵黄とは、卵黄からエタノールやヘキサン等
の溶媒にて卵黄中に含まれるレシチン・中性脂質等の油
脂成分の一部または全部を取り除いたものをいい、通
常、乾燥状に仕上げて、脱脂卵黄粉として市販されてい
る。脱脂卵黄は溶媒処理によって卵黄蛋白質が変性して
おり、清水に不溶となっているため、従来、家畜や稚魚
の飼料にしか用途がなかったものである。In the present invention, the yolk means, in addition to raw egg yolk obtained by breaking a shell egg to remove egg white, frozen yolk obtained by freezing raw yolk, yolk powder obtained by drying raw yolk, and the like. .
In addition, defatted egg yolk refers to those obtained by removing some or all of the oil and fat components such as lecithin and neutral lipids contained in the egg yolk with a solvent such as ethanol or hexane from the egg yolk, and is usually finished in a dry state. Commercially available as defatted egg yolk powder. Since defatted egg yolks have denatured egg yolk proteins due to solvent treatment and are insoluble in fresh water, they have been conventionally used only for feed for livestock and fry.
本発明の実施に当って、まず原料の卵黄又は脱脂卵黄
を容易する。卵黄が冷凍卵黄の場合、予め解凍しておく
とよい。また卵黄が卵黄粉の場合又は脱脂卵黄が乾燥状
の場合にはそのまま用いればよい。In the practice of the present invention, the raw yolk or defatted yolk is first facilitated. If the yolk is a frozen yolk, it may be thawed in advance. When the yolk is yolk powder or when the defatted yolk is dry, it may be used as it is.
次に、この原料に硫安水溶液を加えて撹拌し、この水
溶液中に原料に含まれているホスビチンを溶出させる。
ホスビチンの溶出を効率よく行なうには、乾燥卵黄や脱
脂卵黄を用いる場合には、70%飽和硫安水溶液を加え、
50〜300rpmで3〜24時間撹拌する事が望ましい。また生
卵黄を用いる場合には、最終硫安濃度が70%飽和になる
ように100%飽和硫安水溶液を加えた後この水溶液を80
℃〜100℃で15分程加熱し、卵黄蛋白質を凝固させるの
が望ましい。Next, an aqueous solution of ammonium sulfate is added to the raw material and stirred to elute phosvitin contained in the raw material into the aqueous solution.
In order to efficiently elute phosvitin, when using dried egg yolk or defatted egg yolk, add a 70% saturated ammonium sulfate aqueous solution,
It is desirable to stir at 50 to 300 rpm for 3 to 24 hours. When using raw egg yolk, add a 100% saturated ammonium sulfate aqueous solution so that the final ammonium sulfate concentration becomes 70% saturated, and then add this aqueous solution to 80%.
It is desirable to heat the egg yolk protein for about 15 minutes at -100 ° C to coagulate the yolk protein.
次に、ホスビチンを溶出した硫安水溶液から硫安水溶
液に不溶な凝集物を除く。凝集物を除くには、低速遠心
分離法や濾過法を採用すればよい。Next, aggregates insoluble in the aqueous ammonium sulfate solution are removed from the aqueous ammonium sulfate solution from which phosvitin has been eluted. Aggregates can be removed by low-speed centrifugation or filtration.
最後に、上記凝集物を除いた水溶液からホスビチンを
採取する。ホスビチンを採取するには、例えば、水溶液
をpH1.5〜1.8に調整してホスビチンを沈殿させ、これを
水溶液から分離する方法や、水溶液を希塩酸に対して透
析してホスビチンを沈殿させ、これを水溶液から分離す
る方法などがある。Lastly, phosvitin is collected from the aqueous solution from which the aggregates have been removed. In order to collect phosvitin, for example, the aqueous solution is adjusted to pH 1.5 to 1.8 to precipitate phosvitin and separated from the aqueous solution, or the aqueous solution is dialyzed against dilute hydrochloric acid to precipitate phosvitin. There is a method of separating from an aqueous solution.
以上のように本発明の卵黄ホスビチンの抽出方法は、
撹拌、沈殿及び分離工程の組み合せからなるものであ
り、大型タンクや遠心分離機を用いて工業的に行なうこ
とができ、卵黄ホスビチンの大量生産が可能である。そ
して、このようにして得られた卵黄ホスビチンは極めて
簡便な工程で抽出されるにも拘らず、純度の高い(95%
以上)ものである。As described above, the method of extracting yolk phosvitin of the present invention comprises:
It consists of a combination of agitation, precipitation and separation steps, can be industrially performed using a large tank or a centrifuge, and can mass-produce egg yolk phosvitin. And although the yolk phosvitin thus obtained is extracted by an extremely simple process, it has a high purity (95%
Above).
実施例1 脱脂卵黄粉(キユーピー(株)製、商品名「ヨークプ
ロテインH」)100gに70%飽和硫安水溶液2を加え
て、200rpmで24時間撹拌した。Example 1 A 70% saturated aqueous solution of ammonium sulfate 2 was added to 100 g of defatted egg yolk powder (trade name "York Protein H" manufactured by KP Corporation), followed by stirring at 200 rpm for 24 hours.
次に、この水溶液を3,000rpmで15分間遠心分離して凝
集物を除いた後、No.5C濾紙で濾過した。Next, this aqueous solution was centrifuged at 3,000 rpm for 15 minutes to remove aggregates, and then filtered with No. 5C filter paper.
次に、得られた濾液を透析チューブ(カット分子量約
10,000)に詰め、約20倍量の0.04N塩酸に対し5〜6℃
で24時間透析した後、3,000rpmで15分間遠心分離し、得
られた沈殿物を0.04Nの塩酸で洗浄し、さらに蒸留水に
対して透析後凍結乾燥して卵黄ホスビチン740mgを得
た。Next, the obtained filtrate is passed through a dialysis tube (cut molecular weight of about
10,000), 5 to 6 ° C for about 20 times the volume of 0.04N hydrochloric acid
, And centrifuged at 3,000 rpm for 15 minutes. The obtained precipitate was washed with 0.04N hydrochloric acid, further dialyzed against distilled water, and freeze-dried to obtain 740 mg of egg yolk phosvitin.
実施例2 脱脂卵黄粉(実施例1で用いたものと同じ)100gに70
%飽和硫安水溶液2を加え、200rpmで24時間撹拌し
た。Example 2 70 g of 100 g of defatted egg yolk powder (same as used in Example 1)
2% saturated ammonium sulfate aqueous solution 2 was added and stirred at 200 rpm for 24 hours.
次に、この水溶液を3,000rpmで15分間遠心分離して凝
集物を除いた後、No.5C濾紙で濾過した。そして、濾液
のpHを1.8に調整してホスビチンを凝集させた後、3,000
rpmで15分間遠心分離して沈殿物を回収し、この沈殿物
を蒸留水に対して透析後、凍結乾燥したところ、卵黄ホ
スビチン664mgを得た。Next, this aqueous solution was centrifuged at 3,000 rpm for 15 minutes to remove aggregates, and then filtered with No. 5C filter paper. Then, after adjusting the pH of the filtrate to 1.8 and aggregating phosvitin, 3,000
The precipitate was collected by centrifugation at rpm for 15 minutes, and the precipitate was dialyzed against distilled water and freeze-dried to obtain 664 mg of egg yolk phosvitin.
実施例3 原料として卵黄粉(キユーピー(株)製、商品名「卵
黄粉」)10gを用い、実施例1と同じ方法でこの卵黄粉
を処理して卵黄ホスビチン106mgを得た。Example 3 Using 10 g of yolk powder (trade name "yolk powder", manufactured by KUP Co., Ltd.) as a raw material, the yolk powder was treated in the same manner as in Example 1 to obtain 106 mg of yolk phosvitin.
実施例4 原料として卵黄粉(実施例3で用いたものと同じ)10
gを用い、実施例2と同じ方法でこの卵黄粉を処理して
卵黄ホスビチン30mgを得た。Example 4 Egg yolk powder as raw material (same as used in Example 3) 10
This yolk powder was treated in the same manner as in Example 2 to obtain 30 mg of yolk phosvitin.
実施例5 原料として殻付卵を割卵して得た生卵黄20gを用い、
これに蒸留水40ml飽和硫安水溶液70mlを加え、100℃で1
5分間加熱した。次に3,000rpmで15分間遠心分離して浮
上した凝集物を除いて、下層液を得た。この下層液をN
o.5Cの濾紙で濾過し、濾液を実施例1と同様に処理した
ところ、卵黄ホスビチン93mgを得た。Example 5 As raw material, 20 g of raw egg yolk obtained by breaking shelled eggs was used.
To this, add 40 ml of distilled water and 70 ml of a saturated aqueous solution of ammonium sulfate, and add
Heat for 5 minutes. Next, centrifugation was performed at 3,000 rpm for 15 minutes to remove the floating aggregates, thereby obtaining a lower layer liquid. This lower layer solution is
The solution was filtered through o.5C filter paper, and the filtrate was treated in the same manner as in Example 1 to obtain 93 mg of egg yolk phosvitin.
実施例6 原料として、生卵黄(実施例5で用いたものと同じ)
20gを用い、実施例5と同じ方法で下層液を得た。この
下層液を実施例2と同じ方法で処理したところ、卵黄ホ
スビチン27mgを得た。Example 6 Raw egg yolk as raw material (same as used in Example 5)
Using 20 g, a lower layer solution was obtained in the same manner as in Example 5. This lower layer solution was treated in the same manner as in Example 2 to obtain 27 mg of egg yolk phosvitin.
実施例1乃至実施例6で得られた卵黄ホスビチンをテ
スト区とし、また、生卵黄83gに等量の0.16N食塩水を加
え、20,000rpmで30分間遠心分離し、得られた沈殿物を1
60gの0.16N食塩水中に分散させ、次にこの懸濁液を10,0
00rpmで30分間遠心分離して沈殿物を得、この沈殿物を9
5gの1N食塩水中に溶解させた後、この溶液に226gの100
%飽和硫安水溶液を加え、而る後、この水溶液を20,000
rpmで15分間遠心分離して濾過を得、この濾液から実施
例1と同じ方法でホスビチンを採取し(公知のJ.Agric
Food Chemに準する方法)、得られた卵黄ホスビチンを
対照区とした。The yolk phosvitin obtained in Examples 1 to 6 was used as a test group, 83 g of raw egg yolk was added with an equal volume of 0.16N saline, and the mixture was centrifuged at 20,000 rpm for 30 minutes.
Disperse in 60 g 0.16 N saline, then add this suspension to
Centrifuge at 00 rpm for 30 minutes to obtain a precipitate,
After dissolving in 5 g of 1N saline, 226 g of 100
% Saturated ammonium sulfate aqueous solution,
Filtration was performed by centrifuging at 15 rpm for 15 minutes, and phosvitin was collected from this filtrate in the same manner as in Example 1 (known J. Agric
Food Chem), and the obtained egg yolk phosvitin was used as a control.
そして、上記各卵黄ホスビチンの収率を算出すると共
に、その純度を測定したところ、表−1の結果が得られ
た。And the yield of each said yolk phosvitin was calculated and the purity was measured, and the result of Table 1 was obtained.
尚、収率は原料中に含まれる卵黄ホスビチン含量の理
論値に対する得られた卵黄ホスビチンの百分率(重量
比)である。また、純度は、SDSポリアクリルアミドゲ
ル電気泳動の結果と拘束液体クロマトグラフィ(陰イオ
ン交換クロマト使用)の結果より測定した。The yield is a percentage (weight ratio) of the obtained yolk phosvitin with respect to the theoretical value of the content of yolk phosvitin contained in the raw material. The purity was measured from the results of SDS polyacrylamide gel electrophoresis and the results of constrained liquid chromatography (using anion exchange chromatography).
〔発明の効果〕 以上述べたように、本発明によれば、簡便な方法によ
り純度の高い卵黄ホスビチンを収率よく得ることができ
る。そして、卵黄ホスビチンを大型タンクや遠心分離機
を用いて大量生産ができるので、卵黄ホスビチンを安価
に提供することが可能である。また、原料としてこれま
であまり用途がなかった脱脂卵黄を用いて卵黄ホスビチ
ンを得ることができるという利点も有する。 [Effects of the Invention] As described above, according to the present invention, high-purity egg yolk phosvitin can be obtained with a high yield by a simple method. And since yolk phosvitin can be mass-produced using a large tank or a centrifuge, it is possible to provide yolk phosvitin at low cost. Also, there is an advantage that egg yolk phosvitin can be obtained using defatted egg yolk, which has not been used so far as a raw material.
Claims (1)
拌し、この水溶液中にホスビチンを溶出させた後水溶液
から凝集物を除き、次に、この水溶液からホスビチンを
採取することを特徴とする卵黄ホスビチンの抽出方法。(1) An aqueous solution of ammonium sulfate is added to egg yolk or defatted egg yolk, stirred, phosvitin is eluted in the aqueous solution, aggregates are removed from the aqueous solution, and phosvitin is collected from the aqueous solution. Egg yolk phosvitin extraction method.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1191291A JP2664999B2 (en) | 1989-07-26 | 1989-07-26 | Egg yolk phosvitin extraction method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1191291A JP2664999B2 (en) | 1989-07-26 | 1989-07-26 | Egg yolk phosvitin extraction method |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH0356500A JPH0356500A (en) | 1991-03-12 |
JP2664999B2 true JP2664999B2 (en) | 1997-10-22 |
Family
ID=16272124
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP1191291A Expired - Lifetime JP2664999B2 (en) | 1989-07-26 | 1989-07-26 | Egg yolk phosvitin extraction method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP2664999B2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002256000A (en) * | 2001-02-28 | 2002-09-11 | Transgenic Inc | Antibody for specifically recognizing vitellogenin of bird and vitellogenin assay system of bird |
-
1989
- 1989-07-26 JP JP1191291A patent/JP2664999B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
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JPH0356500A (en) | 1991-03-12 |
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