DE2537123A1 - Albumin recovery from blood fractions - by first treating with an anionic ion exchanger, then selective denaturation of other proteins - Google Patents

Albumin recovery from blood fractions - by first treating with an anionic ion exchanger, then selective denaturation of other proteins

Info

Publication number
DE2537123A1
DE2537123A1 DE19752537123 DE2537123A DE2537123A1 DE 2537123 A1 DE2537123 A1 DE 2537123A1 DE 19752537123 DE19752537123 DE 19752537123 DE 2537123 A DE2537123 A DE 2537123A DE 2537123 A1 DE2537123 A1 DE 2537123A1
Authority
DE
Germany
Prior art keywords
soln
proteins
fraction
denaturation
contg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE19752537123
Other languages
German (de)
Inventor
Alan Cooke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New Zealand Inventions Development Authority
Original Assignee
New Zealand Inventions Development Authority
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New Zealand Inventions Development Authority filed Critical New Zealand Inventions Development Authority
Priority to DE19752537123 priority Critical patent/DE2537123A1/en
Publication of DE2537123A1 publication Critical patent/DE2537123A1/en
Application status is Pending legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76Albumins
    • C07K14/765Serum albumin, e.g. HSA
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K38/00Medicinal preparations containing peptides

Abstract

Prepn. of blood albumin (I) comprises first sepg. a (I)-contg. fraction from blood protein, serum or plasma by treatment with a anionic ion-exchange resin having amino, alkylamino, guanidino or quat. ammonium exchanging gps. An aq. soln. of this separated fraction is prepd., an agent (II) capable of stabilising (I) against heat-denaturation is added, then the soln. heated to denature proteins other than (I). The denaturated proteins are removed leaving a soln. of (I). The stabiliser is >=1 of acetyltryptophan; middle chainlength fatty acids or the salts of these cpds. (esp. Na octanoate). Pref. the resin is crosslinked regenerated cellulose or crosslinked dextran contg. diethylaminoethyl gps. I) is used as a plasma-volume extender and to treat hypoproteinaemia. Using the ion exchange treatment gives a more conc. (I) fraction almost completely free of gamma-globulins (which tend to resist the heat-denaturation step), so less unwanted protein has to be removed.
DE19752537123 1975-08-20 1975-08-20 Albumin recovery from blood fractions - by first treating with an anionic ion exchanger, then selective denaturation of other proteins Pending DE2537123A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE19752537123 DE2537123A1 (en) 1975-08-20 1975-08-20 Albumin recovery from blood fractions - by first treating with an anionic ion exchanger, then selective denaturation of other proteins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19752537123 DE2537123A1 (en) 1975-08-20 1975-08-20 Albumin recovery from blood fractions - by first treating with an anionic ion exchanger, then selective denaturation of other proteins

Publications (1)

Publication Number Publication Date
DE2537123A1 true DE2537123A1 (en) 1977-03-03

Family

ID=5954433

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19752537123 Pending DE2537123A1 (en) 1975-08-20 1975-08-20 Albumin recovery from blood fractions - by first treating with an anionic ion exchanger, then selective denaturation of other proteins

Country Status (1)

Country Link
DE (1) DE2537123A1 (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2742150A1 (en) * 1976-09-20 1978-03-23 Monsanto Co A process for the production of serum albumin
EP0367220A2 (en) * 1988-10-31 1990-05-09 The Green Cross Corporation Albumin preparation and process for preparing the same
US5277818A (en) * 1988-10-31 1994-01-11 The Green Cross Corporation Albumin preparation and process for preparing the same
US5616691A (en) * 1994-06-01 1997-04-01 The Green Cross Corporation Process for producing albumin preparation
US6034221A (en) * 1992-09-23 2000-03-07 Delta Biotechnology Limited High purity albumin
US7993877B2 (en) 1999-01-30 2011-08-09 Novozymes Biopharma Dk A/S Process for the purification of recombinant albumin

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2742150A1 (en) * 1976-09-20 1978-03-23 Monsanto Co A process for the production of serum albumin
EP0367220A2 (en) * 1988-10-31 1990-05-09 The Green Cross Corporation Albumin preparation and process for preparing the same
EP0367220A3 (en) * 1988-10-31 1991-10-09 The Green Cross Corporation Albumin preparation and process for preparing the same
US5277818A (en) * 1988-10-31 1994-01-11 The Green Cross Corporation Albumin preparation and process for preparing the same
US6034221A (en) * 1992-09-23 2000-03-07 Delta Biotechnology Limited High purity albumin
US5616691A (en) * 1994-06-01 1997-04-01 The Green Cross Corporation Process for producing albumin preparation
US7993877B2 (en) 1999-01-30 2011-08-09 Novozymes Biopharma Dk A/S Process for the purification of recombinant albumin
US9029102B2 (en) 1999-01-30 2015-05-12 Novozymes Biopharma Dk A/S Process for the purification of recombinant albumin
US9555344B2 (en) 1999-01-30 2017-01-31 Albumedix A/S Process for the purification of recombinant albumin

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