JPH0352954B2 - - Google Patents
Info
- Publication number
- JPH0352954B2 JPH0352954B2 JP60118954A JP11895485A JPH0352954B2 JP H0352954 B2 JPH0352954 B2 JP H0352954B2 JP 60118954 A JP60118954 A JP 60118954A JP 11895485 A JP11895485 A JP 11895485A JP H0352954 B2 JPH0352954 B2 JP H0352954B2
- Authority
- JP
- Japan
- Prior art keywords
- cells
- cell
- culture solution
- culture
- hollow fiber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000012510 hollow fiber Substances 0.000 claims description 66
- 239000006185 dispersion Substances 0.000 claims description 27
- 238000004113 cell culture Methods 0.000 claims description 22
- 239000012528 membrane Substances 0.000 claims description 16
- 239000006285 cell suspension Substances 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 12
- 230000004663 cell proliferation Effects 0.000 claims description 10
- 238000000926 separation method Methods 0.000 claims description 8
- 238000007667 floating Methods 0.000 claims description 7
- 238000005192 partition Methods 0.000 claims description 7
- 230000010261 cell growth Effects 0.000 claims description 2
- 238000011144 upstream manufacturing Methods 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 90
- 238000000034 method Methods 0.000 description 25
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 12
- 239000001301 oxygen Substances 0.000 description 12
- 229910052760 oxygen Inorganic materials 0.000 description 12
- 239000012530 fluid Substances 0.000 description 11
- 238000012258 culturing Methods 0.000 description 10
- 239000013543 active substance Substances 0.000 description 9
- 238000013019 agitation Methods 0.000 description 8
- 210000004102 animal cell Anatomy 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 6
- 238000004062 sedimentation Methods 0.000 description 5
- 230000001464 adherent effect Effects 0.000 description 4
- 206010035226 Plasma cell myeloma Diseases 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 201000000050 myeloid neoplasm Diseases 0.000 description 3
- 230000007910 cell fusion Effects 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 210000004408 hybridoma Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 229920006393 polyether sulfone Polymers 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 238000010008 shearing Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 208000008720 Bone Marrow Neoplasms Diseases 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 102000008072 Lymphokines Human genes 0.000 description 1
- 108010074338 Lymphokines Proteins 0.000 description 1
- 239000004695 Polyether sulfone Substances 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 description 1
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 201000006491 bone marrow cancer Diseases 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 210000001985 kidney epithelial cell Anatomy 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000021231 nutrient uptake Nutrition 0.000 description 1
- 229920002492 poly(sulfone) Polymers 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 210000004989 spleen cell Anatomy 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229920002803 thermoplastic polyurethane Polymers 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 229960005356 urokinase Drugs 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60118954A JPS61280270A (ja) | 1985-06-03 | 1985-06-03 | 細胞培養装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60118954A JPS61280270A (ja) | 1985-06-03 | 1985-06-03 | 細胞培養装置 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61280270A JPS61280270A (ja) | 1986-12-10 |
| JPH0352954B2 true JPH0352954B2 (de) | 1991-08-13 |
Family
ID=14749373
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60118954A Granted JPS61280270A (ja) | 1985-06-03 | 1985-06-03 | 細胞培養装置 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61280270A (de) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0302894B1 (de) * | 1986-04-28 | 1992-11-19 | Endotronics Inc. | Zuchtverfahren für leukocyten |
| JPH0644859B2 (ja) * | 1987-02-09 | 1994-06-15 | タバイエスペツク株式会社 | 潅流培養装置 |
| US5656421A (en) * | 1990-02-15 | 1997-08-12 | Unisyn Technologies, Inc. | Multi-bioreactor hollow fiber cell propagation system and method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5642584A (en) * | 1979-09-18 | 1981-04-20 | Asahi Chem Ind Co Ltd | Cell cultivation method |
| JPS575690A (en) * | 1980-06-13 | 1982-01-12 | Toray Ind Inc | Circulation device for culture medium in cultivation tank |
| JPS59175878A (ja) * | 1982-12-14 | 1984-10-04 | バイオ−レスポンス・インコ−ポレ−テツド | 細胞の培養方法及び装置 |
| JPS59175877A (ja) * | 1982-12-15 | 1984-10-04 | バイオ−レスポンス・インコ−ポレ−テツド | 培養方法および培養システム |
-
1985
- 1985-06-03 JP JP60118954A patent/JPS61280270A/ja active Granted
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5642584A (en) * | 1979-09-18 | 1981-04-20 | Asahi Chem Ind Co Ltd | Cell cultivation method |
| JPS575690A (en) * | 1980-06-13 | 1982-01-12 | Toray Ind Inc | Circulation device for culture medium in cultivation tank |
| JPS59175878A (ja) * | 1982-12-14 | 1984-10-04 | バイオ−レスポンス・インコ−ポレ−テツド | 細胞の培養方法及び装置 |
| JPS59175877A (ja) * | 1982-12-15 | 1984-10-04 | バイオ−レスポンス・インコ−ポレ−テツド | 培養方法および培養システム |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS61280270A (ja) | 1986-12-10 |
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