JPH03505402A - cDNA構造遺伝子のライブラリーの作製のためのファージ置換ベクターλSK15 - Google Patents

cDNA構造遺伝子のライブラリーの作製のためのファージ置換ベクターλSK15

Info

Publication number
JPH03505402A
JPH03505402A JP1509793A JP50979389A JPH03505402A JP H03505402 A JPH03505402 A JP H03505402A JP 1509793 A JP1509793 A JP 1509793A JP 50979389 A JP50979389 A JP 50979389A JP H03505402 A JPH03505402 A JP H03505402A
Authority
JP
Japan
Prior art keywords
dna
phage
fragment
vector
size
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP1509793A
Other languages
English (en)
Japanese (ja)
Inventor
ザバロフスキ,エフゲニ レアナドビチ
ザゴルスカヤ,エレナ ゲンナディエフナ
キセレフ,レフ ラボビチ
Original Assignee
インスティテュト モレクルヤルノイ ビオロギイ イメニ ベー.アー.エンゲルガルドタ アカデミイ ナウク エスエスエスエル
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by インスティテュト モレクルヤルノイ ビオロギイ イメニ ベー.アー.エンゲルガルドタ アカデミイ ナウク エスエスエスエル filed Critical インスティテュト モレクルヤルノイ ビオロギイ イメニ ベー.アー.エンゲルガルドタ アカデミイ ナウク エスエスエスエル
Publication of JPH03505402A publication Critical patent/JPH03505402A/ja
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • C12N15/73Expression systems using phage (lambda) regulatory sequences
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP1509793A 1988-08-31 1989-08-29 cDNA構造遺伝子のライブラリーの作製のためのファージ置換ベクターλSK15 Pending JPH03505402A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SU884478259A SU1650699A1 (ru) 1988-08-31 1988-08-31 Фаговый вектор замещени @ К15 дл конструировани библиотек структурных генов к ДНК
SU4478259/31 1988-08-31

Publications (1)

Publication Number Publication Date
JPH03505402A true JPH03505402A (ja) 1991-11-28

Family

ID=21397371

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1509793A Pending JPH03505402A (ja) 1988-08-31 1989-08-29 cDNA構造遺伝子のライブラリーの作製のためのファージ置換ベクターλSK15

Country Status (6)

Country Link
JP (1) JPH03505402A (enrdf_load_stackoverflow)
DE (1) DE3990972C2 (enrdf_load_stackoverflow)
GB (1) GB2230012A (enrdf_load_stackoverflow)
SE (1) SE465577B (enrdf_load_stackoverflow)
SU (1) SU1650699A1 (enrdf_load_stackoverflow)
WO (1) WO1990002188A1 (enrdf_load_stackoverflow)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU694269B2 (en) * 1994-09-22 1998-07-16 Merck Sharp & Dohme Corp. Dna encoding human papillomavirus type 6a

Also Published As

Publication number Publication date
GB2230012A (en) 1990-10-10
GB9009525D0 (en) 1990-07-25
DE3990972C2 (enrdf_load_stackoverflow) 1992-12-24
SE9001560D0 (sv) 1990-04-30
WO1990002188A1 (en) 1990-03-08
SE9001560L (sv) 1990-04-30
SE465577B (sv) 1991-09-30
SU1650699A1 (ru) 1991-05-23

Similar Documents

Publication Publication Date Title
ES2226361T3 (es) Vector de alto rendimiento para la secuenciacion de dna.
US4673640A (en) Regulated protein production using site-specific recombination
US5300431A (en) Positive selection vector for the bacteriophage P1 cloning system
Glover Gene cloning: the mechanics of DNA manipulation
US5075227A (en) Directional cloning
JP2004089197A (ja) 組換えdna技術によってdna、rna、ペプタイド、ポリペプタイド、または蛋白質を取得するための方法
WO1998038299A1 (en) Single step assembly of multiple dna fragments
JP2001500740A (ja) 大きな反復dna配列を安定にクローニングする方法
CN108135949A (zh) 递送媒介物
US5128256A (en) DNA cloning vectors with in vivo excisable plasmids
CA1338137C (en) Dna sequences from lactococcus for the efficient expression and secretion of proteins in gram-positive bacteria
JPS58150517A (ja) Dna配列、組換dna分子およびヒト血清アルブミン様ポリペプチドの製造方法
JP2009523428A (ja) 直鎖状ベクター、宿主細胞およびクローニング法
JPH0527400B2 (enrdf_load_stackoverflow)
AU652920B2 (en) Promoter probe vectors, able to replicate in E.coli, B.subtilis, lactococci and lactobacillus as well as uses thereof
Kirschman et al. Two new tools: multi-purpose cloning vectors that carry kanamycin or spectinomycin/streptomycin resistance markers
Lodish et al. Section 7.1 DNA Cloning with Plasmid Vectors
Zeng et al. Large-insert BAC/YAC libraries for selective re-isolation of genomic regions by homologous recombination in yeast
JPH03505402A (ja) cDNA構造遺伝子のライブラリーの作製のためのファージ置換ベクターλSK15
Newton et al. Analysis of the topology of the cytochrome d terminal oxidase complex of Escherichia coli by alkaline phosphatase fusions
Nuss Deletion mutants of double-stranded RNA genetic elements found in plants and fungi
CN101575601A (zh) 生产偶数200bp梯度DNA分子量标准的超级质粒及制备方法
Hughes et al. Genetic mapping of the amino-terminal domain of bacteriophage T4 DNA polymerase
US5863730A (en) Procedure for the polymerization of nucleic acid sequences and its applications
Sorge Bacteriophage lambda cloning vectors