AU652920B2 - Promoter probe vectors, able to replicate in E.coli, B.subtilis, lactococci and lactobacillus as well as uses thereof - Google Patents

Description

WVO 92/04451 PCT/F190/00204 TITLE OF THE INVENTION PROMOTER PROBE VECTORS, ABLE TO REPLICATE IN E.COII, B.SUBTILIS, LACTOCOCCI AND LACTOBACILLUS AS WELL AS USES THEREOF BACKGROUND OF THE INVENTION Field of the Invention The present invention relates to the field of molecular biology, and, more particularly, to the fields of recombinant genetics and genetic engineering. The invention further relates to DNA sequences, derived from Lactococcus lactis, which are useful as promoters and promoter/secretion promoting signals for heterologous or homologous expression in bacteria.

In another aspect, the invention relates to vectors, such as plasmids, comprising the sequences of the present invention, and to host cells transformed with such vectors. Yet additional aspects of the present invention are related to methods for producing desired heterologous or homologous peptides or proteins employing the sequences, vectors, or transformed hosts of the invention. By means of the invention, greatly improved heterologous and homologous expression and secretion may be achieved.

Description of Related Art The lactic acid bacteria are of great commercial importance for, among other things, their ability to carry out fermentation, a process in which organic compounds serve as both electron donors and electron acceptors. Lactic fermentation reduces pyruvate to lactate in a single step reaction catalyzed by NAD-lii. d lactic dehydrogenase, without gas formation, and is the first stage in cheese manufacture.

SUBSTITUTE SHEET WO 92/04451 PCr/F190/0204 -2- Thus, lactic fermentations are responsible for souring, or acidification, of milk and certain other foods, which allows for anaerobic preservation. Further, these processes are involved in the formation of interesting and desirable food and beverage flavors.

The lactic acid bacteria are thus of significant commercial importance. To-date, however, much of the work involving recombinant genetics has been carried out in other bacteria, such as E. coli. One result of this is that the genetics of the lactic acid bacteria are relatively less well understood or characterized. Inasmuch as there is a great deal of practical knowledge relating to the cultivation of lactic acid bacteria for commercial purposes, a continuing need exists for the application of recombinant genetic techniques to the understanding of these bacteria.

de Vos, Neth. Milk Dairy J. 40:141-154 (1986), and FEMS Microbiol. Rev. 46:281-295 (1987), are review articles which disclose mesophilic lactic streptococcal host-vector systems used in cloning and expression of homologous and heterologous genes. Summaries of the properties of lactic streptococcal host. and cloning vectors are set forth, as well as difficulties and issues related to successful transformation.

Gene cloning strategies also are discussed, and sequences of several lactic streptococcal promoters, ribosome binding sites, and terminators are mentioned.

European patent application publication number 0 157 441 discloses certain shuttle vectors capable of expression in B.

subtilis, E. coli and Streptococcus lactis, containing the replicon from the large Clal fragment of the S. cremoris Wg2 plasmid pWVOI. It is stated by the applicants that these vectors can give improved or new properties to lactic acid bacteria transformed therewith. Examples of the use of this system include the expression of genes for a protease and a chymosin precursor in S. lactis.

SUBSTITUTE SHEET WO 92/04451 PC/F190/00204 -3- A number of other reports have appeared relating to the characterization of S. cremoris Wg2 protease activity. For example, Kok et al., Applied Environmental Microbiol. 50:94- 101 (1985), disclose the cloning of a large (4.3 Md) HindIII fragment from the S. cremoris Wg2 plasmid pWV05, and its expression in B. subtilis. It is reported that this fragment contained two proteolytic proteins, and that it could be expressed in a proteinase-deficient S. lactis strain following protoplast transformation. van der Vossen et al., Applied Environmental Microbiol. 50:540-542 (1985), disclose several shuttle vectors derived from the largest Clal fragment of the S. cremoris Wg2 plasmid pWVO1, and the B. subtilis vector pPL608, carrying the B. pumilus chloramphenico! acetyltransferase (CAT) gene. The authors state that these vectors allow the isolation of promoter and transcription terminator signals in lactic streptococci. However, as pointed out subsequently by van der Vossen et al., Applied Environmental Microbiol. 53:2452 -2457 (1987), fragments with promoter activity could be obtained only via precloning in B.

subtilis, because of the low transformation efficiency of S_ lactis protoplasts. The latter paper discloses the characterization of a number of S. cremoris promoters of various strengths, which were isolated partly via precloning in B. subtilis and partly by direct cloning in S. lactis with a pGKV210 plasmid. Several S. cremoris promoter nucleotide sequences are disclosed. Kok et al., Applied Environmental Microbiol. 54:239-244 (1988), disclose a deletion analysis of the S. cremoris Wg2 proteinase gene cloned in the heterologous host S. lactis. van de Guchte et al., Applied Environmental Microbiol. 55:224-228 (1989), disclose the construction of a pair of vectors for expression of heterologous genes in Lactococcus lactis, containing a multiple cloning site flanked by gene expression signals originating from L. cremoris Wg2.

This system was used to express a fusion gene containing the SUBSTITUTE SHEET WO 92/04451 PCr/F190/00204 -4eukaryotic hen egg white lysozyme (HEL) coding sequence in L.

lactis. However, no lysozyme activity was detected from the expressed fusion protein, and the authors state that this was because the fusion protein was either inactive or was produced in too low a quantity to be detected.

Simons et al., J. Dairy Sci. 71 (Supp 1):Abstr. D64 (1988), discloses efficient expression vectors based upon the cryptic S. lactis plasmid pSH71 replicon and lactic streptococcal specific expression signals. It is stated that sequences resembling postulated E. coli and B. subtilis concensus sequences have been identified and function very efficiently in these hosts. It is further stated that these expression signals have been used for synthesis in lactic streptococci of f-galactosidase and chymosin.

Vos et al., J. Dairy Sci. 71 (SupD 1):Abstr. D65 (1988), discloses that S. cremoris SK11 contains a non-bitter cell wall-associated proteinase, of which the complete gene has been cloned and sequenced. It is stated that a DNA fragment containing this gene and another proteinase gene was cloned into a lactic streptococcal cloning vector (pNZ521) and expressed.

Kondo, J. Dairy Sci. 71 (Supp 1):Abstr. D125 (1988), discloses that development of gene transfer and host-vector systems in lactic streptococci have made it possible to study the genetics and plasmid biology of these industrially significant bacteria. It is stated that, basically, four methods of gene transfer exist: transduction, conjugation, protoplast fusion and transformation/transfection. It also is stated that shuttle vector systems for cloning and analysis of lactic streptococcal genes in Escherichia coli, Bacillus subtilis and Streptococcus sanquis allow for detailed molecular analysis of genes and gene products.

de Vos, J. Dairy Sci. 71 (Supp 1):Abstr. D127 (1988), discloses that recently established host-vector systems have SUBSTITUTE

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WO 92/04451 PCT/F190/00204 been used to study the organization and expression of plasmid located genes in mesophilic lactic streptococci S. lactis and S. cremoris. It is stated that most attention has been focussed on homologous genes important for use of these strains in industrial fermentation, and on heterologous genes which could be used to construct strains having novel properties. It also is stated that homologous genes encoding lactose and casein degradation events have been analyzed, as well as regulatory control of copy number of S. lactis plasmid pSH71, and that topogenic sequences which direct cellular location of expressed proteins have been identified.

Despite attempts by others, as illustrated by the preceeding discussion, there continues to exist a need for improved means and methods of homologous and especially heterologous expression in Gram-positive bacteria. Moreover, the present inventors are not aware of the existence of any significant studies involving the Lactococcus lactis subsp.

lactis system.

SUMMARY OF THE INVENTION Accordingly, the present inventors have discovered, isolated, cloned and sequenced novel promoters and promoter/secretion promoting signals from Lactococcus lactis subsp. lactis, which are useful in the production of heterologous and homologous proteins and peptides in E. coli and, especially, in Gram-positive bacteria.

In the course of elucidating the novel sequences of the present invention, it was necessary to design and construct certain vectors which would act as probes to identify L.

lactis subsp. lactis promoters, and which were themselves novel. Accordingly, one embodiment of the present invention provides for a promoter probe-vector able to replicate in SUBSTITUTE SHEET WO 92/04451 PCT/F190/00204 -6- E. coli, B. subtilis, Lactococci and Lactobacillus, selected from the group consisting of the plasmids pKTH1734 and pKTH1736, the said plasmids constructed as shown in Figure or a functional derivative thereof. There is also provided the previously mentioned promoter probe-vector, further comprising rultiple cloning sites having nucleotide sequences as shown in Figure 1, or a functional derivative thereof.

Also provided is a promoter probe-vector able to replicate in E. coli, B. subtilis, Lactococci and Lactobacillus, comprising the plasmid pKTH1750, or a functional derivative thereof.

E. coli, B. subtilis, Lactococci and Lactobacillus hosts transformed with any of these promoter probe-vectors comprise an additional embodiment of the invention.

Using the promoter probe-vectors of the invention, the present inventors were able to clone and sequence previously unknown and undescribed L. lactis subsp. lactis promoter and promoter/secretion signal promoting nucleotide sequences.

Thus, in another embodiment, the present invention provides for a substantially pure nucleotide sequence as shown in Figures 9, 10, 11, 12, 13, 14, 15, 16, 17 or 19, or a functional or chemical derivative thereof. These sequences may be beneficially incorporated into plasmids, by means of which it has been possible to achieve enhanced heterologous protein expression in E. coli and, especially, in Grampositive bacteria. Plasmids comprising these nucleotide sequences thus form another embodiment of the present invention.

Among the sequences and plasmids of the present invention are those which include L. lactis subsp. lactis-derived promoter sequences, exemplified by the sequences found in plasmids pKTH1789, pKTH1816, pKTH1817, pKTH1820, pKTH1821 and pKTH1874. Other sequences and plasmids of the invention include both the promoter and the secretion promoting signals, and are exemplified by the sequences found in plasmids SUBSTITUTE

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WO 92/04451 PCr/F190/00204 -7pKTH1797, pKTH1798, pKTH1799, pKTH1801, pKTH1805, pKTH1806, pKTH1807 and pKTH1809. These plasmids and their respective nucleotide sequences form additional embodiments of the present invention.

In addition to the sequences and plasmids described above, however, an important teaching of the present invention is the discovery by the present inventors that the regulatory elements of those sequences and plasmids may be recombined to produce hybrid expression units which can function together to allow enhanced heterologous expression in E. coli and, especially, in Gram-positive bacteria. Thus, in another embodiment, there is provided according to the present invention a hybrid expression unit composed of a promoter sequence, exemplified by the any of the sequences found in plasmids.pKTH1789, pKTH1816, pKTH1817, pKTH1820, pKTH1821 and pKTH1874, together with a secretion promoting signal derived from sequences and plasmids of the invention including both the promoter and the secretion promoting signals, such as are exemplified by the sequences found in plasmids pKTH1797, pKTH1798, pKTH1799, pKTH1801, pKTH1805, pKTH1806, pKTH1807 and pKTH1809. In one illustrative non-limiting embodiment, there is provided a hybrid expression unit wherein the promoter sequence is derived from the plasmid pKTH1817, and wherein the secretion signal sequence is derived from the plasmid pKTH1807.

In another aspect, the present invention is directed to E. coli and, especially, to Gram-positive host cells transformed with any of the sequences or plasmids of the invention. In accordance with the invention, of course, the plasmids may additionally comprise a nucleotide sequence encoding one or more homologous or heterologous proteins or peptides which it is desired to express primarily in a Grampositive host. Host cells according to the invention are SUBSTITUTE SHEET WO 92/04451 PC/F90/00204 -8selected from the group consisting of E. coli and the Grampositive B. subtilis, Lactococci and Lactobacillus hosts.

An additional embodiment of the present invention provides for a method of heterologous or homologous protein or peptide expression, comprising transforming E. coli or a Grampositive host cell with a plasmid according to the invention (which plasmid also comprises the nucleotide sequence encoding the desired protein or peptide); culturing the transformed host cell in a suitable medium under conditions allowing expression of said protein or peptide, and recovering the expressed protein or peptide from said host cell or said medium.

These embodiments, as well as additional embodiments of the present invention, will become more apparent and easily understood to those of skill by reference to the following detailed description of the invention.

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1: Oligonucleotides used in cloning multiple cloning sites (MCS) in the vector pKTH1736.

Figure 2: The size of in vitro synthesized #-lactamase precursors. Lane 1, f-lactamase control; lane 2, pKTH1797; lane 3, pKTH1798; lane 4, pKTH1799; lane 5, pKTH1801; lane 6, Mr standard. See text for technical details.

Figure 3: mRNAs of L. lactis subsp. lactis promoter constructions (panel A) and promoter signal sequence constructions (panel B) obtained by Northern hybridization.

Panel A: mRNAs were isolated from promoter constructions pKTH1816 pKTH1817 pKTH1820 and pKTH1821 and probed with labeled pPL603. To visualize the bands, X-ray film was exposed 1 h. Panel B: mRNAs were isolated from promoter signal sequence constructions pKTH1805 pKTH1806 pKTH1807 and pKTH1809 as a probe labeled pKTH78 SUBSTITUTE SHEET WO 92/04451 P~J/F90/00204 -9was used. To visualize the bands, the film was exposed overnight.

Figure 4: Construction of vector pKTH1722.

Figure 5: Construction of vectors pKTH1734 and pKTH1736.

Figure 6: Promoter probe vector pKTH1750.

Figure 7: Construction of vectors pKTH1797, pKTH1798, pKTH1799 and pKTH1801 based upon pKTH33, and of vectors pKTH1805, pKTH1806, pKTH1807 and pKTH1809 based upon pVS2.

Figure 8: Identification of the 5' end of mRNAs of L_ lactis subsp. lactis promoter constructions by primer extension. Promoters were from constructions pKTH1817 (panel A, lane pKTH1820 (panel A, lane pKTH1821 (panel B, lane and pKTH1816 (panel B, lane The standard sequence in panel A was from promoter in construction pKTH1817 and in panel B from promoter in construction pKTH1816.

Figure 9: Sequence of pK(TH1816. The black dot above the sequences indicates the start site of mRNA; if it is in parenthesis it indicates a possible secondary start site (this is true generally for all figures showing plasmic sequences where applicable).

Figure 10: Sequence of pKTH1817.

Figure 11: Sequence of pKTH1820.

Figure 12: Sequence of pKTH1874.

Figure 13: Sequence of pKTH1789.

Figure 14: Sequence of pKTH1797.

Figure 15: Sequence of pKTH1798.

Figure 16: Sequence of pKTH1799.

Figure 17: Sequence of pKTH1801.

Figure 18: 01igonucleotide primers used in the construction of the hybrid vector of Figure Figure 19: Sequence of pKTH1821.

Figure 20: Construction of hybrid vector pKTH1889.

SUBSTITUTE SHEET WO 92/04451 Pcr/F190/00204 DESCRIPTION OF THE PREFERRED EMBODIMENTS In the following description, reference will be made to various methodologies known to those of skill in the art of molecular genetics and biology. Publications ?nd other materials setting forth such known methodologies to which reference is made are incorporated herein by reference in their entireties as though set forth in full.

Standard reference works setting forth the general principles of recombinant DNA technology include Watson, J.D.

et al., Molecular Biology of the Gene, Volumes I and II, The Benjamin/Cummings Publishing Company, Inc., publisher, Menlo Park, CA (1987); Darnell, J.E. et al., Molecular Cell Biology, Scientific American Books, Inc., publisher, New York, N.Y.

(1986); Lewin, Genes II, John Wiley Soun publishers, New York, N.Y. (1985); Old, et al., Prirciples of Gene Manipulation: An Introduction to Genetic Engineering, 2d edition, University of California Press, publisher, Berkeley, CA (1981); and Maniatis, et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, publisher, Cold Spring Harbor, NY (1982). General principles of microbiology are set forth, for example, in Davis, B.D. et al., Microbiology, 3d edition, Harper Row, publishers, Philadelphia, PA (1980).

By "promoter" is meant generally a region on a DNA molecule to which an RNA polymerase binds and initiates transcription. The nucleotide sequence of the promoter determines both the nature of the enzyme that attaches to it and the rate of RNA synthesis. As used herein, "promoter" preferably refers to nucleotide sequences derived from L la:tis subsp. lactis. Similarly, by "promoter/signal promoting sequence" is meant generally a nucleotide sequence which comprises, in addition to a promoter sequence, a SUBSTITUTE SHEET WO 92/04451 PC/F190/00204 -11sequence encoding a 16-35 amino acid segment, usually containing hydrophobic amino acids that become embedded in the lipid bilayer membrane, which allows for the secretion of an accompanying protein or pepFide sequence from the host cell, and which usually is cleaved from that protein or peptide.

As used herein, "promoter/signal promoting sequence" preferably refers to nucleotide sequences derived from L.

lactis subsp. lactis.

By "hybrid expression unit" is meant any combination of the promoter and promoter/signal promoting sequences jf the invention to produce a different or distinct sequence which retains expression or expression and secretion functions. The manner and methods of combining the sequences of the invention to produce numerous such hybrid expression units are well known to those of skill, and are described and exemplified herein. Further, those skilled in the art who have fully appreciated the teachings of the present invention will recognize that it will be possible and even desirable to produce such hybrid expression units in order to optimize expression and secretion of given heterologous or homologous proteins or peptides, and that the same will be accomplished using well-known recombinant methods with the exercise of merely routine skill.

By "cloning" is meant the use of in vitro recombination techniques to insert a particular gene or other DNA sequence into a vector molecule. In order to successfully clone a desired gene, it is necessary to employ methods for generating DNA fragments, for joining the fragments to vector molecules, for introducing the composite DNA molecule into a host cell in which it can replicate, and for selecting the clone having the target gene from amongst the recipient host cells.

By "cDNA" is meant complementary or copy DNA produced from an RNA template by the action of RNA-dependent DNA polymerase (reverse transcriptase). Thus, a "cDNA clone" SUBSTITUTE SHE'aT WO 92/04451 PCr/F19000204 -12means a duplex DNA sequence complementary to an RNA molecule of interest, carried in a cloning vector.

By "cDNA library" is meant a collection of recombinant DNA molecules containing cDNA inserts which together comprise the entire genome of an organism. Such a cDNA library may be prepared by methods known to those of skill, and described, for example, in Maniatis et al., Molecular Cloning: A Laboratory Manual, supra. Generally, RNA is first isolated from the cells of an organism from whose genome it is desired to clone a particular gene. Preferred for the purposes of the present invention are cell lines of bacteria.

By "vector" is meant a DNA molecule, derived from a plasmid or bacteriophage, into which fragments of DNA may be inserted or cloned. A vector will contain one or more unique restriction sites, and may be capable of autonomous replication in a defined host or vehicle organism such that the cloned sequence is reproducible. Thus, by "DNA expression vector" is meant any autonomous element capable of replicating in a host independently of the host's chromosome, after additional sequences of DNA have been incorporated into the autonomous element's genome. Such DNA expression vectors include bacterial plasmids and phages. Preferred for the purposes of the present invention, however, are plasmids comprising promoters and promot'.. secretion promoting sequences derived from L. lactis.

By "substantially pure" is meant any protein of the present invention, or any gene encoding any such protein, which is essentially free of other proteins or genes, respectively, or of other contaminants with which it might normally be found in nature, and as such exists in a form not found in nature. This term also may be used with reference to the nucleotide sequences encoding the promoters and promotersecretion promoting sequences of the invention derived from L lactis. By "functional derivative" is meant the "fragments," SUBSTITUTE SHEET WO 92/04451 PC/F190/00204 -13- "variants," "anal or "chemical derivatives" of a molecule. A "fragment" of a molecule, such as any of the DNA sequences of the present invention, is meant to refer to any nucleotide subset of the molecule. A "variant" of such molecule is meant to refer to a naturally occurring molecule substantially similar to either the entire molecule, or a fragment thereof. An "analog" of a molecule is meant to refer to a non-natural molecule substantially similar to either the entire molecule or a fragment thereof.

A molecule is said to be "substantially similar" to another molecule if the sequence of amino acids in both molecules is substantially the same. Substantially similar amino acid molecules will possess a similar biological activity. Thus, provided that two molecules possess a similar activity, they are considered variants as that term is used herein even if one of the molecules contains additional amino acid residues not found in the other, or if the sequence of amino azid residues is not identical. As used herein, a molecule is said to be a "chemical derivative" of another molecule when it contains additional chemical moieties not normally a part of the molecule. Such moieties may improve the molecule's solubility, absorption, biological half life, etc. The moieties may alternatively decrease the toxicity of the molecule, eliminate or attenuate any undesirable side effect of the molecule, etc. Moieties capable of mediating such effects are disclosed, for example, in Remington's Pharmaceutical Sciences, 16th ed., Mack Publishing Co., Easton, Penn. (1980).

Similarly, a "functional derivative" of a gene encoding any of the molecules of the present invention is meant to include "fragments," "variants," or "analogues" of the gene, which may be "substantially similar" in nucleotide sequence, and which encode a molecule possessing similar activity.

SUBSTITUTE SHEET WO 92/04451 PCT/F190/00204 -14- A nucleic acid molecule, such as DNA, is said to be "capable of expressing" a polypeptide if it contains nucleotide sequences which contain transcriptional and translational regulatory information and such sequences are "operably linked" to nucleotide sequences which encode the polypeptide. An operable linkage is a linkage in which the regulatory DNA sequences and the DNA sequence sought to be expressed are connected in such a way as to permit gene expression. The precise nature of the regulatory regions needed for gene expression may vary from organism to organism, but shall in general include a promoter region which, in prokaryotes, contains both the promoter (which directs the initiation of RNA transcription) as well as the DNA sequences which, when transcribed into RNA, will signal the initiation of protein synthesis. Such regions will normally include those 5'-non-coding sequences involved with initiation of transcription and translation, such as the TATA box, Shine- Dalgarno sequence, and the like.

If desired, the non-coding region 3' to the gene sequence coding for the protein may be obtained by the above-described methods. This region may be retained for its transcriptional termination regulatory sequences, such as termination. Thus, by retaining the 3'-region naturally contiguous to the DNA sequence coding for the protein, the transcriptional termination signals may be provided. Where the transcriptional termination signals are not satisfactorily functional in the expression host cell, then a 3' region functional in the host cell may be substituted.

Two DNA sequences (such as a promoter region sequence and a heterologous protein encoding sequence) are said to be operably linked if the nature of the linkage between the two DNA sequences does not result in the introduction of a frame-shift mutation, interfere with the ability of the promoter region sequence to direct the transcription of the SUBSTITUTE SHEET '0 92/04451 PCY/F190100204 heterologous protein gene sequence, or interfere with the ability of the heterologous protein gpne sequence to be transcribed by the promoter region ,ience. Thus, a promoter region would be operably linked to a DNA sequence if the promoter were capable of effecting transcription of that DNA sequence.

Thus, to express the protein, transcriptional and translational signals recognized by an appropriate host are necessary.

In a preferred embodiment, the introduced sequence will be incorporated into a plasmid vector capable of autonomous replication in the recipient host. Any of a wide variety of vectors may be employed for this purpose. Factors of importance in selecting a particular plasmid vector include: the ease with which recipient cells that contain the vector may be recognized and selected from those recipient cells which do not contain the vector; the number of copies of the vector which are desired in a particular host; and whether it is desirable to be able to "shuttle" the vector between host cells of different species. Preferred prokaryotic vectors include plasmids such as t ose capable of replication in E.

coli (such as, for example, pBR322, ColE1, pSC101, pACYC 184, 7rVX). Such plasmids are, for example, disclosed by Maniatis, et al. (In: Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Press, Cold Spring Harbor, NY (1982)). Bacillus plasmids include pC194, pC221, pT127, etc. Such plasmids are disclosed by Gryczan, T. (In: The Molecular Biology of the Bacilli, Academic Press, NY (1982), pp. 307-329).

Particularly preferred vectors according to the invention are those which are able to replicate in E. coli, B. subtilis, Lactococci and Lactobacillus.

Once the vector or DNA sequence containing the construct(s) has been prepared for expression, the vector or DNA construct(s) may be introduced into an appropriate host cell al jDCTiTI ITF SHFFT WO 92/04451 PCT/F190/100204 -16by any of a variety of suitable means, including such biochemical means as transformation, transfection, conjugation, protoplast fusion, calcium phosphateprecipitation, and application with polycations such as diethylaminoethyl (DEAE) dextran, and such mechanical means as electroporation, direct microinjection, and microprojectile (biolistic) bombardment (Johnston et al., Science 240(4858): 1538 (1988)), etc.

After the introduction of the vector, recipient cells are grown in a selective medium, which selects for the growth of vector-containing cells. Expression of the cloned gene sequence(s) results in the production of the desired heteroloJus or homologous protein, or in the production of a fragment of this protein.

The expressed protein may be isolated and purif 4 ed in accordance with conventional conditions, such as extraction, precipitation, chromatography, affinity chromatography, electrophoresis, or the like. For example, the cells may be collected by centrifugation, or with suitable buffers, lysed, and the protein isolated by column chromatography, for example, on DEAE-cellulose, phosphocellulose, polyribocytidylic acid-agarose, hydroxyapatite or by electrophoresis or immunoprecipitation. In a preferred embodiment, the expressed protein will also be secreted from the host cell when any of the promoter/secretion promoting signals of the invention are employed, with the advantage that isolation and purification procedures will be simplified.

Alternatively, the expressed heterologous protein or functional derivative thereof, may be isolated by the use of antibodies directed against the desired protein or functional derivative. Such antibodies may be obtained by well-known methods.

SUBSTITUTE SHEET WO 92/04451 PCT/FI90/00204 -17- The manner and method of carrying out the present invention may be more fully understood by those of skill by reference to the following examples, which examples are not intended in any manner to limit the scope of the present invention or of the claims directed thereto.

EXAMPLE I BACTERIAL STRAINS AND GROWTH MEDIA The bacterial strains used are listed in Table 1.

Table 1 Bacterial Strains, Genotypes and Sources Strain E. coli TG1 B. subtilis Lactococcus lactis subsp. lactis MG1614 Our Code ERF173 BRB1 Genotype K12A(lac pro) supE thi hsdD5 proA+, B+ laqlI lacZ M15 metB5 sacA321 Reference or Source

EMBL

(European Molecular Biology Laboratory) Palva I., Gene 19:81- 87 (1982) GRS5 transformable Valio 2 Gasson M.

Lactobacillus plantarum NRLB192 Valio 'Formerly called Streptococcus lactis.

2 Valio Finnish Co-operative Dairies' Association.

SUBSTITUTE SHEET WO 92/04451 PCr/F190/00204 For propagating E. coli and B. subtilis strains, Luria broth (Lennox, Virology 1:190-206 (1955)) was used; for L.

lactis M17G or M17GS broth (Terzaghi et al., Appl. Microbiol.

29:807-813 (1975)) and for L. olantarum, MRS broth (De Man et al., J. Appl. Bacteriol. 23:130-135 (1960)) was used.

Table 2 Media and Markers Used fo Various Hosts Host E. coli ERF173 B. subtilis BRB1 Growth Luria Luria Antibiotic 50 pg/ml or 11 pg/ml 12,5 pg/ml 5 'g/ml, pg/ml L. lactis GRS5 M17G, M17GS cm 4-5 pg/ml cm 4-5 pg/ml L. plantarum MRS ORIGINATING PLASMIDS pVS2 shuttle vector between E. coli, lactis, L. plantarum kb, emr, cmr Described by von Wright et al., Microbiol. 53:1584-1588 (1987).

pAMB11 Bacillus vector, 5.3 kb, kmr, cmr Described by Zukowski et al., (1986)).

B. subtilis, Appl. Environm.

(Gene 46:247-255 pKTH33 A deletion derivative of pHV33, a chimera between pBR322 and pC194.

SUBSTITUTE SHEET WO 92/04451 PCT/F190/00204 -19- Described by Palva, Ph.D. thesis, University of Helsinki (1983).

4.6 kb, apr, cmr in E. coli, cmr in B. subtilis (Michel et al., Gene 12:147-154 (1980)).

pSH71 Cryptic L. lactis plasmid, 2 kb Described by Gasson, J. Bacteriol. 154:1-9 (1983).

pBR322 4.4 kb, apr, tcr Described by Sutcliffe, Cold Spring Harbor Symp. Quant. Biol. 43:77 (1979); and Peden, K.W.C., Gene 22:277 (1983).

pPL603 Promotor cloning vector for B. subtilis 4.8 kb, kmr Described by Duval et al., J. Bacteriol. 158:784-790 (1984).

pK1H78 Bacillus vector 5.5 kb, kmr Contains TEM--lactamase gene.

Described by Palva et al., Proc. Natl. Acad. Sci.

USA 79:5582-5586 (1982).

METHODOLOGY USED DNA Isolations and Modifications Rapid isolation of plasmid DNA from E. coli for screening of the clones was done according to, Holmes et al. (Anal.

Biochem. 134:193-197 (1980)). DNA for restriction enzyme digests was prepared by the method of Birnboim et al. (Nucl.

Acids Res. 7:1513-1523 (1979)) either from 1 ml or 10 ml of liquid culture. RNAse (Boehringer) was added prior to restriction enzyme treatments.

SUBSTITUTE SHEET WO 92/04451 PCT/F190/00204 Isolation of plasmid DNA from B. subtilis was carried out according to Gryczan et al. Bacteriol. 134:318-329 (1978)). Isolation of plasmid DNA from L. lactis subsp.

lactis was carried out according to Andersson et al., (Appl.

Environm. Microbiol. 46:549-552 (1983)), both for small-scale and large-scale isolations.

Chromosomal DNA from L. lactis subsp. lactis was isolated by the above-described method; only the chromosomal band was collected from CsCl-runs.

Further purification of DNA, if needed, was done by CsCl- EtBr density gradient centrifugation, regardless of the source of the DNA preparation.

Restriction enzyme digestions were performed according to the manufacturer's recommendations (Boehringer, BRL, Promega).

Selected restriction fragments were obtained by separation of the digested DNA on 0.8% agarose gel electrophoresis (Sharp et al., Biochemistry 12:3055-3063 (1973)) after which DNA extraction and purification was performed by a phenol-liquid nitrogen freezing method as follows: a slice of agarose containing the desired fragment was transferred to a siliconized Eppendorf tube and mashed with a glass rod. About 250 ul TE-buffer was added together with an equal volume of phenol. After thorough mixing in a Vortex shaker, the tube was immersed in liquid nitrogen until frozen. The phases were separated by centrifugation at 1200 rpm for 15 minutes, after which phenol extraction was repeated and the resulting aqueous layer treated with ether and ethanol-precipitated.

As an alternative method for DNA fragment isolation, the procedure described by Hawkins et al. (Curr. Genet. 9:305-311 (1985)) was used, or the isolation was done by electroelution with a "Model UEA Undirectional Electroelutor Analytical" apparatus (International Biotechnologies, Inc.) according to the manufacturer's instructions.

SUBSTITUTE SHEET WO 92/04451 PCY1/F190/00204 -21- Modification of the Ends of DNA Fragments For the generation of blunt-end fragments, the Klenow fragment of DNA polymerase I (Promega) was used. As alternative methods, T4 DNA polymerase (Promega) or mung bean nuclease (Promega) also were used. For dephosphorylation of ends, calf intestinal phosphatase (CIP, Boehringer) was used. T4 polynucleotide kinase (Promega) was used for phosphorylation of the 5'-hydroxyl ends.

The ends of the DNA fragments were joined by T4 DNA ligase (Promega). All modifying enzymes were used according to manufacturer's recommendations.

DNA Transformations Transformation of E. coli cells was accomplished by the method of Hanahan Mol. Biol. 166:557-580 (1983)). B.

subtilis cells were transformed by the method of Gryczan et al. Bacteriol. 134:318-329 (1978)). L. lactis protoplast transformation was carried out according to von Wright et al.

(Appl. Environm. Microbiol. 50:1100-1102 (1985)). L.

plantarum transformation by electroporation was performed by the method of Aukrust et al. (submitted for publication).

The method is described below.

Lactobacillus plantarum transformation by Electroporation Electroporation. For electroporation experiments, cells were grown to an optical density of 0.5-1.0 (A 600 chilled on ice, harvested by centrifugation, washed, and resuspended in electroporation buffer (EB) to a cell density of about 109 cells/mi. An aliquot of 0.8 ml ice-cold cell suspension was mixed with 0.5-1.0 jg of plasmid DNA. Cells were kept on ice before and after electroporation in buffer (PEB).

SUBSTITUTE SHEET WO 92/04451 PCT/F190/00204 -22- Electroporation was performed using a GenePulserTM apparatus (BioRad Laboratories, Richmond, USA) at a constant capacitance of 2b uFD, with a field strength between 1250 and 6250 V/cm for whole cells and between 1250 and 5000 V/cm for osmosensitive clls. Electroporation of intact cells was carried out in EB as described in the GenePulserTM operating instructions (BioRad Laboratories, Richmond, USA).

Osmosensitive cells were electropurated in protoplast electroporatior: buffer (PEB): 0.5 M raffinose, 7 mM sodium phosphate pH 7.4, MgC12 up to 50 mM.

Enzymatic Assays B-lactamase was assayed according to O'Callaghan et al.

(Antimicrob. Aq. Chemother. 1:238-288 (1972)). Cell and supernatant fractions were separated by centrifugation after growth in appropriate liquid medium.

Chloramphenicol acetyltransferase (CAT) assay. Cells were grown to log phase, and 1 ml cultures were collected for enzyme activity analysis. Cells were harvested by centrifugation, washed with 50 mM sodium phosphate buffer pH 7, and suspended in 0.2 ml of the same buffer containing 4 mg/mi lysozyme. Cells were incubated for 30 minutes at 37'C, after which they were disrupted by sonication (4 x 15 seconds, using a Bransonic sonicator; after each 15 seconds of sonication, the medium was cooled for 30 seconds in an ice bath). After sonication, the cell debris was pelleted by centrifugation. 50 pl of the supernatant was used for enzyme assay. CAT-activity was measured according to the method of Shaw, Meth. Enzvmol. 43:737-755 (1975)).

Catechol 2,3-dioxygenase was assayed according to Zukowski et al. (Proc. Natl. Acad. Sci. USA 80:1101-1105 (1983)).

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WO 92/04451 PCr/F190/00204 -23- RNA Methods Isolation of RNA RNA was isolated according to the method of van der Vossen et al. (Appl. Environm. Microbiol. 53:2452-2457 (1987)), except that cells were cultured in 10 ml of M17Gmedium containing 5 pg/ml chloramphenicol until Klett 80 was reached, and that the RNA (and DNA) was precipitated with ethanol (the medium was made to 0.5 M with 3 M NaAc, and 3 volumes of ethanol were added). The pellet was dissolved in distilled water. The DNA was digested with RNAse-free DNAase I (Promega) in 40 mM Tris-HCl (pH 10 mM NaC1, 6 mM MgC12 buffer containing 10 mM dithiothreitol and 40 U/ml ribonuclease inhibitor RNAsinR (Promega). After incubation for 10 minutes at 37'C, the reaction mixture was extracted once with phenol, phenol-chloroform-isoamyl-alcohol (25:24:1, vol/vol) and chloroform-isoamylalcohol (24:1 vol/vol). The RNA was precipitated with ethanol and the pellet was dissolved in 75 p1 of water.

Nortnern Transfer and Hybridization To estimate the length of the RNAs transcribed by the cloned promoter or promoter/signal sequence fragments and to study the strength of the promoters, Northern analysis was made. The RNA gel was run and Northern transfer to nitrocellulose membrane (Schleicher and Schuell) was done according to Williams et al. (in, "Nucleic Acid Hybridization--A Practical Approach," Hames et al. IRL Press, pp. 139-160 (1985)).

To detect the RNA species, the nitrocellulose filter was prehybridized in 0.06 M sodium citrate (4 x SSC), 50 mM sodium phosphate buffer (pH 5 x Denhardt (Biochem. Biophys.

SUBSTITUTE SHEET WO 92/04451 PCT/F190/00204 -24- Res. Commun. 23:641-646 (1966)), 0.2% sodium dodecyl sulphate (SDS), and 200 jg/ml denatured herring sperm DNA (Sigma).

Incubation was done for one to two hours at Hybridization was done in the same medium containing nicktranslated probe (106 cpm/ml). After hybridization, the filter was washed (1-2 x) with 0.03 M sodium citrate (2 x SSC), 0.2% SDS, and incubated at 37°C for 30 minutes and for minutes at Primer Extension The transcriptional start sites were determined by primer extension. To 15 p1 RNA (5 to 10 jg) primer (0.2 pmol of base oligonucleotide) mixture, 15 pl 2 x hybridization buffer (100 mM Tris-HC1, pH 8.3, 2 mM EDTA, 0.8 M NaCl) was added.

The mixture was heated to 95' for 2 minutes and allowed to cool to room temperature over a two-hour period by gradually lowering the thermostat of the water bath.

The RNA-primer hybrid was precipitated with ethanol, and the pellet was dissolved in 5 p1 of 2 x reaction buffer (100 mM Tris-HCl, pH 8.3 at 42°C, 20 mM DTT, 12 mM MgC12, 100 mM KC1, 0.5 mM dATP, dTTP and dGTP and 50 pg/ml actinomycin C 1 (Boehringer)). To this mixture, 1.5 il of deoxicytidine (a- 32 P) triphosphate (3000 Ci/mmol, 10 mCi/ml, Amersham), and U of RNAsinR 7 U AMV Reverse transcriptase (Promega) were added, and the total reaction volume was made to 10 p1 with water. The reaction mixture was incubated for 15 minutes at 42"C, after which 0.5 p1 of 10 mM dCTP (chase) was added, and incubation was continued at 42"C for 1 hour and 45 minutes.

Subsequently, the reaction mixture was extracted with phenol and phenol-chloroform-isoamylalcohol (25:24:1), and precipitated with ethanol.. The reverse transcriptase reactions were analyzed by electrophoresis on a standard sequencing gel. Sequencing reactions of one of the promoter SUBSTITUTE SHEET WO 92/04451 PCT/F190/00204 constructions were used as a size marker and were run in parallel with the reverse tra-scriptase (RT) reactions.

Other Methods In vitro translation of pre-9-lactamase was performed with a DNA expression system kin vitro DNA Directed, Prokaryotic by NEN Products, DuPont), after which the products were separated by SDS-PAGE according to Laemmli (Nature (London) 227:680-685 (1970)) and subjected to fluorography.

DNA sequencinq All DNA sequencing was based on the Sanger method (Proc.

Natl. Acad. Sci. USA 80:3963-3965 (1977)). For plasmid sequencing, a SequenaseTM (United States Biochemical Corporation, USB) system was used, as described by Hattori et al. (Anal. Biochem. 152:232-238 (1986)).

Oliqonucleotide synthesis Oligonucleotide synthesis of primers for sequencing and polymerase chain reactions were performed by phosphoramidite chemistry (Beaucage et al., Tetrahedron Letters 22:1859-1862 (1981)) using Applied Biosystems DNA synthesizer model 381A.

PCR-polymerase chain reaction: Amplification of specific DNA fragments was accomplished by GeneAmpTM DNA Amplification kit as described by Saiki et al. (Science 239:487-491 (1988)) and the DNA Thermal Cycler (both from Perkin Elmer-Cetus).

Taq polymerase was purchased from Perkin Elmer-Cetus.

SUBSTITUTE SHEET WO 92/04451 PCF19000204 -26- EXAMPLE II Construction if a Promoter Probe Vector For the screening of chromosomal DNA fragments containing promoter-like activity, a promoter probe-vector able to replicate in E. coli, B. subtilis, Lactococci and Lactobacillus was constructed.

The replication origin for the shuttle vector was isolated from the plasmid pSH71. The plasmid pSH71 was digested with restriction enzyme Clal to create two fragments of about 1.7 kb and 0.3 kb, the larger one of which contained the replication origin. The sticky ends were filled in with the Klenow fragment. The mixture was run in an agarose gel to isolate the large DNA fragment, and the DNA was eluted from the gel by electroelution.

To the replication fragment, two antibiotic selectio, markers were added: the gene coding for tetracycline was isolated from the plasmid pBR322, and the gene coding for erythromycin resistance was isolated from the plasmid pVS2.

In order to get the gene fragment coding for tetracycline resistance, pBR322 was digested with EcoRI and PvuII. The sticky ends created by EcoRI were filled in with the Klenow fragment, the mixture was run in an agarose gel, the tetracycline gene-containing fragment was isolated, and the DNA fragment (about 2 kb) was eluted from the gel by electroelution.

The Clal fragment containing the pSH71 replication origin and the DNA fragment containing the tetracycline gene were ligated and transformed into competent E. coli ERF173 cells.

Transformants were selected by plating the transformation mixture on Luria-agar plates containing 12.5 gg/ml tetracycline. The structure of the plasmid was verified by SUBSTITUTE SHEET WO 92/04451 PC/F190/0P0204 -27restriction enzyme digestions. To this new plasmid, designated pKTH1722 (Figure the second resistance marker was added. pKTH1722 was linearized by XmnI digestion. The erythromycin gene was isolated from the plasmid pVS2 by HindIII-Clal digestion, and the sticky ends were filled in with the Klenow fragment. The mixture was run in an agarose gel, the gel fragment containing the erythromycin gene was isolated, and the DNA fragment eluted from the gel by electroelution.

The linearized plasmid pKTH1722 and the erythromycin gene-containing DNA fragment were ligated, the ligation mixture was transformed into competent E. coli ERF173 cells, and the mixture was plated on Luria agar plates containing 12.5 gg/ml tetracycline. Transformants were screened by their ability to grow on Luria-agar plates containing 100 #g/ml erythromycin. Plasmid isolation was done from erythromycin resistant colonies and the presence of the gene was verified by restriction enzyme digestions. One correct plasmid construction was named pKTH1734 (Figure For the construction of the promoter probe plasmid, a promoterless gene coding for chloramphenicol acetyltransferase from the plasmid pPL603 was ligated to the plasmid pKTH1734.

pKTH1734 was linearized by EcoRI digestion, and the sticky ends were made blunt by the Klenow fragment. The promoterless cat gene was isolated from the plasmid pPL603 by EcoRI-PvuII digestion, the sticky ends were filled in with Klenowfragment, and the mixture was run on an agarose gel. The cat gene-containing DNA fragment (about 1.7 kb) was isolated by the phenol-liquid nitrogen freezing method as described above.

The linearized plasmid pKTH1734 and the cat genecontaining DNA-fragment were ligated and transformed to E. coli ERF173 cells. The inserts were screened by isolating plasmids and checking the restriction enzyme recognition SUBSTITUTE SHEET WO 92/04451 PCr/F19 0/00 204 -28patterns by digestions. The plasmid pKTH1736 was obtained (Figure To further improve the vector, a DNA fragment containing multiple cloning sites (MCS) was added in front of the cat gene. pKTH1736 was linearized by PstI digestion. The MCSsequence was constructed from two synthetic 21 base singlestranded oligonucleotides (Figure which were annealed in vitro. The ends of the MCS fragment were constructed so that, when ligated to PstI site, only one functional PstI site was formed. After ligation of the MCS segment to linearized pKTH1736, the mixture was transformed into competent E. coli ERF173 cells, and the MCS sequence-containing transformants were screened by isolating plasmids and doing restriction enzyme digestions.

To check that the MCS sequence was present in the vector only once, the plasmid obtained by the above procedure was digested with EcoRI and ligated to itself in a dilute medium and transformed to E. coli ERF173. The promoter probe vector pKTH1750 was obtained from this transformation (Figure 6).

EXAMPLE III Screening for Lactococcus Promoter Fragments by a Promoter Probe Vector DKTH1750 The promoter probe plasmid pKTH1750 can replicate in E. coli, B. subtilis, and L. lactis. The promoters were screened both in B. subtilis and in L. lactis. Lactococcus chromosomal DNA, digested with Sau3A, was ligated with BglIIdigested pKTH1750 in a molar ratio of 2:1 (insert:vector DNA).

The mixture was transformed to L. lactis GRS5 cells and plated on M17GS-cm (4 #g/ml) plates, and also to B. subtilis BRB1 SUBSTITUTE SHEET WO 92/04451 PCT/F190/00204 29 cells and plated on Luria-cm (5 p(spl2hl2vsb6Tg/ml) plates. Only those transformants which contained promoterlike sequences in front of the cat gene could grow on cm plates. The transformants obtained from B. subtilis transformation were further screened by Minimal Inhibitory Concentration assay (MIC). An overnight colony was suspended in a 1 ml 50 mM phosphate buffer pH 7.0. F:om the suspension, a streak was drawn with a glass rod for a set of Luria-cm plates containing different concentrations of cm 15, 45, 100 pg/ml). Only transformants which could grow at a cm concentration of 45 to 100 pg/ml were transformed to L. lactis subsp. lactis Clones which were screened directly from L. lactis subsp. lactis. and clones which were first screened in B. subtilis and then transformed to GRS5, were characterized by CAT assay in both BRBI and GRS5 hosts. Results for the clones pKTH1816 (Figure pKTH1817 (Figure pKTH1820 (Figure 11), pKTH1821 (Figure 19) are shown in Table 3.

Inserts were sequenced according to Sanger's dideoxy method, and were further characterized by Northern hybridization and primer extension.

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PC/F190/00204 WO 92/04451 Table 3 Expression of Chloramphenicol Acetyltransferase in L. lactis subsp. lactis and in B. subtilis Initial Cloning Host Plasmid pKTH1816 GRS5 pKTH1817 GRS5 pKTH1820 BRB1 pKTH1821 BRB1 (control) or BRB1 CAT Activity in L. lactis GRS5 U/ml* 0.015 0.2 0.029 0.012 0.004 CAT in B.subtilis BRB1 U/mi 9.3 45.3 12.5 8.1 0.09 *Activity was measured described herein.

as U/ml of culture medium as EXAMPLE IV Screening for Lactococcus Promoter Fragments by a Promoter Probe Vector pAMB11, Capable of Replication in B. subtilis A promoter probe vector pAMB11 replicating in B. subtilis has been previously described (Zukowski et al., Gene 46:247- 255 (1986)). This plasmid was opened with the restriction enzyme Smal for a blunt-end cloning site or with BamHI to generate sticky ends.

Lactococcus chromosomal DNA was digested with Sau3A, which yielded fairly large (over 1000 bp) fragments, compatible for ligation with the BamHI-treated vector. For SUBSTITUTE

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WO 92/04451 PCT/ 190/0204 -31blunt-ended fragments, the chromosomal DNA was sonicated (Branson Sonifier, Branson Sonic Power Co.) to get 500-600 bp fragments. The extent of sonication was checked by running a small aliquot of treated sample in a 0.8% agarose gel with appropriate controls. The total sonicated DNA was then applied to a 0.8% agarose gel and electrophoresed. Fractions of about 600 bp were extracted and purified by phenol-liquid nitrogen treatment. The ends of the DNA fragments were treated with the Klenow fragment as described above.

The ligations, in both cases, were performed in a molar ratio of 2:1 (insert:vector DNA) under standard conditions, and the mixtures were transformed into B. subtilis BRB1.

Selection for promoter-containing plasmids was done by spraying the bacterial colonies with 0.5 M catechol.

Transformants bearing a promoter sequence turned yellow due to the expression of catechol 2,3-dioxygenase, which converts catechol to 2-hydroxymuconic semialdehyde. The intensity of the yellow color is known to correlate with promoter strength. Plasmids pKTH1874 (Figure 12) and pKTH1789 (Figure 13) were obtained from the above transformations.

To study the production of catechol 2,3-dioxygenase in liquid culture (Table the two strains were grown in Luria broth containing 10 ug/ml kanamycin. After 10 hours growth, 1 ml of cells was collected by centrifugation, treated, and the enzymatic activity determined according to the method described by Zukowski et al. (Proc. Natl. Acad. Sci. USA 80:1101-1105 (1983)).

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WO 92/04451 PCr/F190/00P4 -32- Table 4 Lactococcus Chromosomal DNA Fragments Promoting the Expression of Catechol 2,3-dioxygenase in B. subtilis Formation of 2-hydroxy-Muconic Semialdehyde (Catechol 2,3dioxvaenase) Activity Size of Insert bp Color 1 Intensity Construction pKTH1874 pKTH1789 control 550 500 25.2 mmol/min 207 mmol/min <2 No insert 1See text for details.

2 Below detection limit.

DNA was extracted from the positive clones and subjected to plasmid sequencing.

EXAMPLE V Screening for the Promoter/Signal Sequence Fragments Using Plasmid pKTH33 Plasmid pKTH33 contains the structural part of TEM-#lactamase gene preceded by an EcoRI linker. Part of the plasmid originates from pBR322, allowing its replication in E. co i. If a sequence bearing an expression/secretion signal is inserted, in frame, with the marker gene #-lactamase, active enzyme is produced, which renders the transformants resistant to ampicillin. By plating the transformants SUBSTITUTE SHEET WO 92/04451 PT/F]90/00204 -33directly on ampicillin plates, a positive selection for signal sequence fragments is obtained.

Plasmid pKTH33 was opened with EcoRI, treated with Klenow fragment to obtain blunt-end molecules, and purified by phenol extraction and ethanol precipitation.

The ligation mixture was transformed into E. coli ERF173 cells, and plated on Luria-ampicillin (50 gg/ml) plates.

Several transformants were screened for #-lactamase activity by Nitrocefin assay on microtiter wells: 200 p1 of Nitrocefin (Glaxo) in 50 mM K-phosphate buffer (pH 7.0) were pipetted into microtiter plate wells. Bacterial colonies were transferred from plates with a toothpick and suspended in Nitrocefin. Positive clones turned red after 1-30 minutes incubation at room temperature, whereas negative clones stayed yellow.

The minimal inhibitory concentration (MIC) of ampicillin for the positive clones was determined as described, except that cells were plated or Luria-ap plates containing ampicillin from 50-450 pg/ml. MIC was the highest concentration still supporting growth.

Clones which grew on ampicillin (400 pg/ml or greater) were chosen for further characterization.

Rapid isolation of plasmid DNA was performed on positive clones showing iighest ampicillin resistance. The size of the chromosomal DNA insert was verified by restriction enzyme digests. Clones pKTH1797 (Figure 14), pKTH1798 (Figure pKTH1799 (Figure 16) and pKTH1801 (Figure 17) are shown in Table SUBSTITUTE SHEET WO 92/04451 PCT/F190/00204 -34- Table Plasmids Showing High Resistance to Ampicillin Size of Insert MIC of ap Construction bp Uq/ml pKTH1797 2,000 400 pKTH1798 350 >450 pKTH1799 500 400 pKTH1801 500 >450 Inserts of pKTH1797, pKTH1798, pKTH1799 and pKTH1801 were sequenced according to the dideoxy method of Sanger, and analyzed for the presence of expression/secretion signals. By matching the three reading frames with the known reading frame of P-lactamase, the correct reading frame was determined. The length of the precursor proteins was compared with the data obtained from an in vitro transcription-translation assay (Figure in order to confirm the validity of the sequences.

B-lactamase activity of the four constructions was also determined by growing the appropriate strains in liquid medium (Table 6).

SUBSTITUTE SHEET WO 92/04451 PCT/F190/00204 Table 6 Expression of P-lactamase in E. coli Periplasm U/ml pKTH1797 pKTH1798 pKTH1799 pKTH1801 pBR322 2 ERF173 336 841 74 nd 1 4,103 <3 Cells U/ml 292 155 47 nd 1 42 <3 1 Not determined.

2 The source of the intact #-lactamase gene. The inserts were carried on E. coli vector pKTH33. To study periplasmic B-lactamase, cells were sonicated (4 x 15 seconds, Bransonic sonifier), cell debris was separated by centrifugation, and the supernatant taken as the periplasmic fraction.

Below detection level.

EXAMPLE VI Subcloning of Promoter/Secretion Signal Fragments into a Shuttle Replicon Although the use of pKTH33 allowed direct selection of the desired fragments, the clones could not, as such, be propagated in Gram-positive bacteria. It was therefore necessary to change the replicon by subcloning the promoter/signal sequence fragments into the plasmid pVS2.

SUBSTITUTE SHEET WO 92/04451 PCT/F190/00204 -36- The insert plus the entire #-lactamase gene was cleaved off from pKTH1797, pKTH1798, pKTH1799, and pKTH1801 by Clal- Pvull double digestion, and the desired fragments were extracted from a 0.8% agarose gel as previously described and treated with the Klenow fragment to generate blunt ends. The vector pVS2 was opened with HindIII, and treated with the Klenow fragment as above.

Ligation was performed in a molar ratio of 2:1 (insert:plasmid) under standard conditions, and the mixture was transformed into E. coli ERF173 cells and plated on Luriacm (11 gg/ml) plates. The production of #-lactamase was checked by the Nitrocefin microtiterwell assay, as described.

Rapid isolation of plasmid DNA was done for positive clones, and the size of the insert was verified by restriction enzym2 digests.

Since heterogeneity among subclones was observed, four clones that retained both #-lactamase activity and DNA structure unchanged were selected for further transformations.

The four secretion vectors were designated pKTH1805, pKTH1806, pKTH1807 and pKTH1809 (Figure 7).

EXAMPLE VII Expression and Secretion of B-lactamase in Gram-positive Hosts To test the functioning of the isolated promoter/signal sequence fragments in Gram-positive bacteria, the four different constructions were transformed into B. subtilis BRB1, L. lactis GRS5, and L. plantarum NRLB192. The strains were then grown in liquid culture, under optimal conditions for each specific host.

SUBSTITUTE SHEET PCT/F190/00204 WO 92/04451 -37- Cell and supernatant fractions from 8-10 hour cultivations were subjected to Nitrocefin assay (Table 7).

Table 7 -lactamase Activity in Gram-positive Hosts #-lactamase Activity U/mi B. subtilis L. lactis L. plantarum Sup Cells Sup Cells Sup Cells Construction pKTH1805 pKTH1806 pKTH1807 pKTH1809 Controll 2.6 17.1 2.6 1.6 1.3 24.6 8.2 6.3 5.8 75.2 245 72.6 0 2.5 1.7 57 <2 229.0 10.5 5.3 lEach host strain without a plasmid.

2 Below detection limit.

EXAMPLE IX Estimation of Promoter Strength Promoter strength was initially estimated by comparing the promoter's ability to grow on antibiotic plates (cm plates for strains cloned by promoter probe vector; ap plates for strains cloned by promoter/signal sequence vector), its ability to produce high MIC, or its ability to synthesize large amounts of gene product (chloramphenicol acetyl transferase or P-lactamase).

SUBSTITUTE SHEET WO 92/04451 PCr/F190/00204 -38- Selected clones (promoter clones pKTH1816, pKTH1817, pKTH1820 and pKTH1821) were further studied by Northern hybridization (Figure The results indicated that promoters cloned by promoter probe vector (pKTH1750) produced more test gene (cat) specific mRNA than did promoters cloned together with the signal sequence (the test gene in the latter case was bla). The difference was about 5-10 fold, when the differences between the specific activities of the probes were taken into account. Judged by transcriptional efficiency, the promoters cloned by the cat-plasmid pKTH1750 appeared stronger than promoters cloned together with the signal sequence.

EXAMPLE X Construction of Hybrid Vectors To demonstrate the manner in which the different promoters and signal sequences function together as hybrid expression units according to the present invention, the promoter on the expression/secretion plasmid pKTH1807 was replaced by the promoter on the expression plasmid pKHT1817.

The promoter was taken from plasmid pKTH1817 by the polymerase chain reaction (PCR) technique, using oligonucleotides A and B as primers (Figure 18). Primer B for the 3'-end of the promoter fragment was designed so that, at the end of the PCR fragment, a restriction enzyme recognition site for Xbal was created.

The signal sequence-#-lactamase (bla) region was taken from plasmid pKTH1807 by PCR, using oligonucleotides C and D as primers. The 5'-end primer (primer C) was designed so that a restriction enzyme recognition site for Xbal was created.

SUBSTITUTE SHEET WO 92/04451 PCr/F190/00204 -39- Both the promoter fragment and the signal sequence-bla fragment obtained by PCR were digested with Xbal and purified on an agarose gel. They were ligated (as a 1:1 molar concentration ratio of signal sequence-bla to promoter fragment). The ligation of the Xbal site between the promoter and the signal sequence fra-ments regenerated the authentic and sequences at the joint region. The -igation mixture was digested with BglII and Clal. The digestion mixture was run in an agarose gel, from which the proper fragment--containing the promoter ligated to the signal sequence bla--was isolated. This fragment was amplified with PCR and digested with PvuII. It was ligated to a pVS2-vector, which was digested with HpaII and made blunt by the Klenow enzyme.

Table 8 -lactamase Activity of a Hybrid Construction P-lactamase Activity (U/ml) Construction sup cells pKTH1807 268 1.3 pKTH1889 2,892 4.6 The ligation mixture was transformed into competent E. coli ERF173 cells and plated on Luria-ap (100 jg/ml) plates. Transformants so obtained were streak-d wveral times on ap plates, in order to get stable cultures.

From the clones so obtained, the plasmid was isolated, transformed to L. lactis GRS5 cells, and plated on M17GS-cm Mg/ml) plates. From these transformations, a clone (pKTH1889) was obtained which, as shown in Table 8, produced SUBSTITUTE

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WO 92/04451 PCr/F190/00204 approximately ten times more f-lactamase than L. lactis strain pKTH1807, which contained the original promoter/signal sequence combination.

SUBSTITUTE SHEET

Claims (18)

1. A promoter probe-vector able to replicate in E. coli, B. subtilis, Lactococci and Lactobacillus, compris- ing the plasmid pKTH1736, the said plasmid constructed as shown in Figure 5, or a functional derivative thereof.

2. A promoter probe-vector able to replicate in E. coli, B. subtilis, Lactococci and Lactobacillus, further comprising multiple cloning sites having nucleotide se- quences as shown in Figure 1, or a functional derivative thereof. A promoter probe-vector able to replicate in E. coli, B. subtilis, Lactococci and Lactobacillus, compris- ing the plasmid pKTH1750, or a functional derivative thereof.

4. A host transformed with the promoter probe-vec- tor of any of claims 1, 2 or 3. A substantially pure nucleotide sequence as shown in Figures 9, 10, 11, 12, 13, 14, 15, 16, 17 or 19, or a functional or chemical derivative thereof.

6. A plasmid comprising the nucleotide sequence of claim

7. A plasmid selected from the group consisting of plasmids pKTH1805, pKTH1806, pKTH1807 and pKTH1809, said plasmid constructed as shown in Figure 7, or a functional derivative thereof.

8. A plasmid selected from the group consisting of plasmids pKTH1816, pKTH1817, pKTH1820 and pKTH1821, said plasmid constructed as described in Examples II or III, or a functional derivative thereof.

9. The plasmid of claims 6, 7 or 8, further com- prising a nucleotide sequence encoding a heterologous or homologous protein or peptide which it is desired to ex- press. A Gram-positive host cell transformed with the SUBSTITUTE 'HEET The Swedish Patent Office F 2 0 4 PCT International Application 42 30 -09- 1992 plasmid of claim 9.

11. The host cell of claim 10, wherein said cell is selected from the group consisting of B. subtilis, Lactococci and Lactobacillus.

12. The plasmid pKTH1889, constructed as shown in Figure

13. The plasmid of claim 12, further comprising a homologous or heterologous protein or peptide which it is desired to express.

14. A Gram-positive host cell transformed with the plasmid of claim 13. The host cell of claim 14, wherein said cell is selected from the group consisting of B. subtilis, Lactococci and Lactobacillus.

16. A plasmid comprising a promoter sequence de- rived from Lactococcus lactis, selected from the group consisting of plasmids pKTH1789, pKTH1816, pKTH1817, pKTH1820, pKTH1821 and pKTH1874, or a functional derivat- ive thereof.

17. A plasmid comprising a promoter/secretion sig- nal sequence derived from Lactococcus lactis, selected from the group consisting of pKTH1797, pKTH1798, pKTH1799, pKTH1801, pKTH1805, pKTH1806, pKTH1807 and pKTH1809, or a functional derivative thereof.

18. A hybrid expression unit, comprising a pro- moter sequence of any of the plasmids of claim 16 and a secretion signal sequence of any of the plasmids of claim .17.

19. The hybrid expression unit of claim 18, where- in said promoter sequence is derived from the plasmid pKTH1817, and wherein said secretion signal sequence is derived from the plasmid pKTH1807. The hybrid expression unit of claim 18 or 19, further comprising a homologous or heterologous protein or peptide which i is desired to express.

21. A Gram-positive host cell transformed with the SUBSTITUTE SHEET The Swedish Patent Office PCT International Application 43 L -09- 1992 hybrid expression unit of claim

22. The host cell of claim 21, wherein said cell is selected from the group consisting of B. subtilis, Lactococci and Lactobacillus.

23. A method for producing a desired heterologous or homologous protein or peptide in a Gram-positive host cell, comprising transforming said host cell with the plasmid of claim 9; culturing the transformed host cell in a suitable medium under conditions allowing expression of said protein or peptide, and recovering the expressed protein or peptide from said host cell or said medium. p SUBSTITUTE SHEET