JPH0292268A - Injection device of very small amount to cell tissue of organism - Google Patents
Injection device of very small amount to cell tissue of organismInfo
- Publication number
- JPH0292268A JPH0292268A JP24618888A JP24618888A JPH0292268A JP H0292268 A JPH0292268 A JP H0292268A JP 24618888 A JP24618888 A JP 24618888A JP 24618888 A JP24618888 A JP 24618888A JP H0292268 A JPH0292268 A JP H0292268A
- Authority
- JP
- Japan
- Prior art keywords
- capillary
- small amount
- cell tissue
- solution
- organism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002347 injection Methods 0.000 title abstract description 7
- 239000007924 injection Substances 0.000 title abstract description 7
- 230000001105 regulatory effect Effects 0.000 claims abstract description 11
- 238000000520 microinjection Methods 0.000 claims description 2
- 239000000243 solution Substances 0.000 abstract description 16
- 241000255789 Bombyx mori Species 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 229940057995 liquid paraffin Drugs 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Infusion, Injection, And Reservoir Apparatuses (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は例えばカイコの卵などのような生体細胞組織に
微量の溶液を注射するための生体細胞組織への微量注射
装置に関する。DETAILED DESCRIPTION OF THE INVENTION (Industrial Application Field) The present invention relates to a microinjection device for a living cell tissue, for injecting a small amount of solution into a living cell tissue such as silkworm eggs.
（従来の技術）
従来のこの種装置においては溶液を満たしたキャピラリ
イを注射筒やゴム球に接続してその注射筒やゴム球内の
空気圧により、キャピラリイ内の溶液を押し出す方法か
或いは溶液を満たしたキャピラリイをパイプを介して流
動パラフィン等の油性の溶液を満たしたシリンジに接続
してシリンジ内の流体圧によりキャピラリイ内の溶液を
押し出す方法が一般に用いられていた。(Prior art) In conventional devices of this type, a capillary filled with a solution is connected to a syringe or a rubber bulb, and the solution in the capillary is pushed out using air pressure inside the syringe or rubber bulb. A commonly used method is to connect a capillary filled with liquid paraffin via a pipe to a syringe filled with an oily solution such as liquid paraffin, and use the fluid pressure in the syringe to push out the solution in the capillary.
（発明が解決しようとする課題）
しかしながらこれらの方法は、いずれもキャピラリイ内
の溶液を押し出すための圧力を手の調整で行うため正確
さに限界があると共にこの時、キャピラリイを生体細胞
組織内へ刺し込むための位置の調整は圧力調整を行う片
手と異なる他方片手でのみ行うこと＼なり、結果として
操作が不正確になり組織を傷付は易い等の欠点があり、
また後者のシリンジ内の流体圧による押し出し方法では
溶液が油に接するため汚染される恐れがあり、そのため
組織に害を与えるものを注射することになる等の欠点が
あった。(Problem to be Solved by the Invention) However, in all of these methods, the pressure to push out the solution in the capillary is manually adjusted, so there is a limit to accuracy, and at this time, the capillary is not connected to the living cell tissue. Adjustment of the position for inserting the needle into the body must be done with the other hand, which is different from the one used to adjust the pressure.As a result, there are disadvantages such as inaccurate operation and easy damage to the tissue.
Furthermore, in the latter extrusion method using fluid pressure within a syringe, the solution comes into contact with oil and may become contaminated, which has the disadvantage of injecting something harmful to the tissue.
本発明はか＼る欠点を改良することを目的とする。The present invention aims to improve these drawbacks.
（課題を解決するための手段）
上記の目的を達成するための本発明の構成を実施例に対
応する図面に基づいて説明すると本発明は溶液を満たし
たキャピラリイ（５）をフートスイッチ式３方電磁弁（
４）と調圧弁（２）とを介して空気圧力源（１）に接続
したことを特徴とする。(Means for Solving the Problems) The structure of the present invention for achieving the above object will be explained based on the drawings corresponding to the embodiments. Solenoid valve (
4) and a pressure regulating valve (2), the air pressure source (1) is connected to the air pressure source (1).
（作 用）
そして本発明は上記の手段によりキャピラリイ（５）を
油圧式マイクロマニュピユレータ−で操作しつ＼顕微鏡
下で生体細胞組織に刺し込み次いで足でフートスイッチ
式３方電磁弁（４）を操作することにより所定量の溶液
が生体細胞組織内に注入される。(Function) According to the above-described method, the capillary (5) is operated by a hydraulic micromanipulator, inserted into a living cell tissue under a microscope, and then operated with a foot switch type three-way solenoid valve. By operating (4), a predetermined amount of solution is injected into the living cell tissue.
（実施例）
以下図面について本発明の詳細な説明すると（１）はボ
ンベからなる空気圧力源を示し、該空気圧力源（１）に
は２段式の第１：Ａ圧弁（２）とニードルバルブ（３）
と１段式の第２調圧弁（２）とフートスイッチ式３方電
磁弁（４）とを順次接続し、該３方電磁弁（４）は第２
図に示すように電磁作動により摺動する弁体（４ａ）に
より開閉される給入口（４ｂ）と戻し口（４ｃ）と出力
口（４ｄ）とを有し、その出力口（４ｄ）に溶液を満た
したキャピラリイ（５）を接続した。図中（６）はフー
トスイッチ式３方電磁弁（４）のフートスイッチ、（７
）はキャピラリイ（５）を操作する油圧式マイクロマニ
ピュータ、（８）は各調圧弁（２）の圧力計を示す。(Example) To explain the present invention in detail with reference to the drawings below, (1) shows an air pressure source consisting of a cylinder, and the air pressure source (1) includes a two-stage first: A pressure valve (2) and a needle. Valve (3)
A one-stage second pressure regulating valve (2) and a footswitch-type three-way solenoid valve (4) are connected in sequence, and the three-way solenoid valve (4) is connected to the second pressure regulating valve (2).
As shown in the figure, it has a supply port (4b), a return port (4c), and an output port (4d) that are opened and closed by a valve body (4a) that slides by electromagnetic operation. A capillary (5) filled with water was connected. In the figure, (6) is the foot switch of the foot switch type 3-way solenoid valve (4), and (7)
) indicates a hydraulic micromanipulator that operates the capillary (5), and (8) indicates the pressure gauge of each pressure regulating valve (2).
かくてキャピラリイ（５）の先端の直径と調圧弁（２）
による空気圧力源の空気圧の調整とフートスイッチ式３
方電磁弁（４）の注入時間の設定とにより、キャピラリ
イ（５）内の溶液の注射量は正確に決めることができ、
このときキャピラリイ（５）内にかけられた空気圧は３
方電磁弁（４）のためスイッチ（６）か切れたとき即座
にその戻し口（４ｃ）より解除されるため、スイッチ（
６）を切った直後に溶液の流出が止まり、余分の溶液を
注射することがなく正確に注入量を調整でき、また注射
時に両手でキャピラリイ（５）を操作したま＼、その注
入量は足で操作されるフートスイッチ（６）により行わ
れるため溶液の注入が従来の方法に比べ迅速に行うこと
ができると共に生体細胞組織内のキャピラリイ（５）の
位置の、より微細な調整を行いながら、しかも組織に損
傷を与えずに行える。Thus, the diameter of the tip of the capillary (5) and the pressure regulating valve (2)
Adjustment of air pressure of air pressure source and foot switch type 3
By setting the injection time of the solenoid valve (4), the injection amount of the solution in the capillary (5) can be determined accurately.
At this time, the air pressure applied inside the capillary (5) was 3
Since the solenoid valve (4) is immediately released from the return port (4c) when the switch (6) is turned off, the switch (
Immediately after turning off the capillary (5), the solution stops flowing out, allowing you to accurately adjust the injection volume without injecting excess solution. Since it is performed by a foot-operated foot switch (6), the solution can be injected more quickly than conventional methods, and the position of the capillary (5) within the living cell tissue can be more finely adjusted. However, it can be done without damaging the tissue.
（発明の効果）
このように本発明によるときは、溶液を満たしたキャピ
ラリイ（５）をフートスイッチ式３方電磁弁（４）と調
圧弁（２）とを介して空気圧力源（１）に接続したもの
であるからキャピラリイ（５）内への空気圧は調整弁（
２）により容易に調整されると共にその供給時間は３方
電磁弁（４）のフートスイッチ（６）の操作により正確
に調整され而もキャピラリイ（５）の操作は両手で行う
ことができるため生体細胞組織を損傷することがない等
の効果を有する。(Effects of the Invention) According to the present invention, the capillary (5) filled with a solution is connected to the air pressure source (1) via the foot switch type three-way solenoid valve (4) and the pressure regulating valve (2). Since it is connected to the capillary (5), the air pressure inside the capillary (5) is controlled by the regulating valve (
2), the supply time can be adjusted accurately by operating the foot switch (6) of the three-way solenoid valve (4), and the capillary (5) can be operated with both hands. It has the effect of not damaging living cell tissues.
図面は本発明の一実施例を示すもので第１図は概略図、
第２図は３方電磁弁の縦断面図である。
（１）・・・空気圧力源 （２）・・・調圧弁（４
）・・・フートスイッチ式３方電磁弁（５）・・・キャ
ピラリイ
第１図
第２図
Ｃ
外３名The drawings show one embodiment of the present invention, and FIG. 1 is a schematic diagram;
FIG. 2 is a longitudinal sectional view of the three-way solenoid valve. (1)... Air pressure source (2)... Pressure regulating valve (4
)...Foot switch type 3-way solenoid valve (5)...Capillary Figure 1 Figure 2 C 3 other people
Claims (1)
磁弁と、調圧弁とを介して空気圧力源に接続したことを
特徴とする生体細胞組織への微量注射装置。A microinjection device for biological cell tissue, characterized in that a capillary filled with a solution is connected to an air pressure source via a footswitch type three-way solenoid valve and a pressure regulating valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24618888A JPH0292268A (en) | 1988-09-30 | 1988-09-30 | Injection device of very small amount to cell tissue of organism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24618888A JPH0292268A (en) | 1988-09-30 | 1988-09-30 | Injection device of very small amount to cell tissue of organism |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0292268A true JPH0292268A (en) | 1990-04-03 |
| JPH0355101B2 JPH0355101B2 (en) | 1991-08-22 |
Family
ID=17144824
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP24618888A Granted JPH0292268A (en) | 1988-09-30 | 1988-09-30 | Injection device of very small amount to cell tissue of organism |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0292268A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03290180A (en) * | 1990-04-05 | 1991-12-19 | Hitachi Air Conditioning & Refrig Co Ltd | Culture medium-pouring device |
| US7499087B2 (en) | 2002-06-13 | 2009-03-03 | Panasonic Corporation | Noise reducing device and noise reducing method |
-
1988
- 1988-09-30 JP JP24618888A patent/JPH0292268A/en active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03290180A (en) * | 1990-04-05 | 1991-12-19 | Hitachi Air Conditioning & Refrig Co Ltd | Culture medium-pouring device |
| US7499087B2 (en) | 2002-06-13 | 2009-03-03 | Panasonic Corporation | Noise reducing device and noise reducing method |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0355101B2 (en) | 1991-08-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |