JPH0355101B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial Application Field) The present invention relates to a microinjection device for a living cell tissue, for injecting a small amount of solution into a living cell tissue such as silkworm eggs.

(Prior art) In conventional devices of this type, a capillary filled with a solution is connected to a syringe or a rubber bulb, and the air pressure inside the syringe or rubber bulb is used to push out the solution in the capillary, or the solution is filled. A commonly used method is to connect a capillary with a syringe filled with an oily solution such as liquid paraffin via a pipe, and to force the solution in the capillary into the syringe using fluid pressure.

(Problem to be Solved by the Invention) However, in all of these methods, the pressure to push out the solution in the capillary is manually adjusted, so there is a limit to accuracy. Adjustment of the position for insertion must be done only with one hand, which is different from the one used to adjust the pressure, which has disadvantages such as inaccurate operation and easy damage to the tissue, and the fluid pressure inside the latter. In the extrusion method, there is a risk of contamination because the solution comes into contact with oil, which has the disadvantage of injecting substances that are harmful to the tissue.

The present invention aims to improve these drawbacks.

(Means for Solving the Problems) To achieve the above object, the present invention provides a three-way solenoid valve having a valve body for switching an air outlet to communicate with an air inlet or an air outlet, and a three-way solenoid valve having a valve body for switching an air outlet to communicate with an air inlet or an air outlet; It is characterized by comprising a capillary connected to the air outlet, an air pressure source connected to the air inlet via a pressure regulating valve, and a foot switch for switching the three-way solenoid valve.

(Function) According to the above-described method, the capillary 5 is operated by a hydraulic micromanipulator, the capillary 5 is inserted into a living cell tissue under a microscope, and the foot switch type three-way solenoid valve 4 is operated by the foot. A fixed amount of solution is injected into living tissue.

(Embodiment) An embodiment of the present invention will be described below with reference to the drawings. Reference numeral 1 indicates an air pressure source consisting of a cylinder, and the air pressure source 1 includes a two-stage first pressure regulating valve 2, a needle valve 3, and a one-stage A second pressure regulating valve 2 and a footswitch type three-way solenoid valve 4 are connected in sequence, and the three-way solenoid valve 4 slides by electromagnetic operation as shown in FIG. 2, and has a valve body having upper and lower contact surfaces. 4a, an air inlet 4b and an air outlet 4c that are opened and closed by the valve body 4a, and a valve body 4.
An air outlet 4d is provided, which is switched to communicate with the air inlet 4b or the discharge port 4c by movement of the air outlet a, and a capillary 5 filled with a solution is connected to the air outlet 4d. In the figure, reference numeral 6 indicates a foot switch of the foot switch type three-way solenoid valve 4, reference numeral 7 indicates a hydraulic micromanipulator for operating the capillary 5, and reference numeral 8 indicates a pressure gauge for each pressure regulating valve 2.

Thus, the diameter of the tip of the capillary 5 and the pressure regulating valve 2
By adjusting the air pressure of the air pressure source and setting the injection time of the footswitch type three-way solenoid valve 4, the injection amount of the solution in the capillary 5 can be determined accurately.
The air pressure applied to the capillary 5 through
When the switch 6 for the solenoid valve 4 is turned off, it is immediately released through the air outlet 4d and the discharge port 4c, so the outflow of the solution stops immediately after the switch 6 is turned off, making it possible to inject excess solution. The amount of injection can be adjusted accurately without any problems, and since the amount of injection is controlled by the foot switch 6 operated by the foot while operating the capillary 5 with both hands during injection, the solution can be injected more quickly than with conventional methods. In addition, the position of the capillary 5 within the living cell tissue can be more finely adjusted without damaging the tissue.

(Effects of the Invention) According to the present invention, since the capillary 5 filled with a solution is connected to the air pressure source 1 via the footswitch type three-way solenoid valve 4 and the pressure regulating valve 2, The air pressure to the air can be easily adjusted by the regulating valve 2, and the supply time can be accurately adjusted by operating the foot switch 6 of the 3-way solenoid valve 4, and since the capillary 5 can be operated with both hands, it is possible to It has the effect of not damaging the

[Brief explanation of drawings]

The drawings show one embodiment of the present invention, and FIG. 1 is a schematic diagram, and FIG. 2 is a longitudinal sectional view of a three-way solenoid valve. 1... Air pressure source, 2... Pressure regulating valve, 4... Foot switch type 3-way solenoid valve, 5... Capillary.

Claims (1)

[Claims] 1. A three-way solenoid valve having a valve body that switches the air outlet to communicate with the air inlet or the discharge port, a capillary filled with the solution to be injected and connected to the air outlet, and a capillary connected to the air inlet via a pressure regulating valve. 1. A microinjection device for biological cell tissue, comprising an air pressure source and a foot switch for switching the three-way solenoid valve.