JPH03290180A - Culture medium-pouring device - Google Patents

Abstract

PURPOSE:To obtain the title device capable of effectively utilizing operation space on a germ-free operation table, carrying out operation well and having good efficiency of pouring operation by pouring a culture medium into a culturing vessel in the germ-free operation table from the outside thereof in a specific constitution. CONSTITUTION:A culture medium pouring vessel table 6 capable of folding is provided outside a germ-free operation table in which clear stream is communicated and simultaneously a culture medium vessel 8 is put on the pouring vessel table 6. A pouring pipe 11 is passed from a culture medium sucking and feeding vessel means connected to the culture medium vessel 8 through the side wall of germ-free operation table into a culturing vessel 10 in the germ-free operation table. Thereby it is made possible to pour a culture medium 9 contained in the culture medium vessel 8 in the outside of germ-free operation table through a pouring pipe 11 into the culturing vessel 10 in the germ-free operation table when culture medium sucking and feeding means is operated.

Description

[Detailed Description of the Invention] [Industrial Field of Application] The present invention relates to a culture medium dispensing device, which makes use of the work space on a sterile workbench and allows one worker to perform dispensing work without using both hands. This invention relates to a medium dispensing device suitable for.

[Prior Art] In recent years, tissue culture of plants and the like has been booming, and culture containers are often sterilized by gamma rays or ethylene oxide gas.

Conventionally, a medium for growing seedlings from tissues of plants, etc.
There were two methods for dispensing into a large number of culture vessels:

One method is to put the culture medium into a container such as an Erlenmeyer flask, stopper it with aluminum foil, etc., sterilize it in a high-pressure steam sterilizer, and then culture it using a pipette etc. on a sterile workbench with clean airflow. This is a method of dispensing into containers.

The other method was to put the culture medium into a dispenser, sterilize the dispenser with a high-pressure steam sterilizer, place it on a sterile workbench, and dispense.

These dispensing operations must be performed before the temperature of the medium drops and the agar, which is the coagulant for the medium, solidifies.

[Problem to be solved by the invention] Generally, it is necessary to dispense the culture medium before the agar hardens.
The temperature of the medium itself during the dispensing operation is close to 100°C.

Therefore, it is dangerous to transfer to a container with one hand, and conventionally, when operating a pipette or dispenser, both hands must be used. The problem was that it was inefficient.

Furthermore, when dispensing with a dispenser, the dispenser itself has to be placed on a sterile workbench and there is a problem that the workspace on the sterile workbench on which the culture container is placed becomes small.

The present invention was made in order to solve the problems of the above-mentioned prior art, and it makes the work space on the sterile workbench more efficient.
It is an object of the present invention to provide a medium dispensing device that allows an operator to work without inconvenience and improves the efficiency of medium dispensing work.

[Means for Solving the Problems] In order to achieve the above object, the structure of the culture medium dispensing device according to the present invention is such that a culture medium is supplied from outside the sterile work bench to a culture container located in a sterile work bench through which a clean airflow flows. This media dispensing device includes a foldable dispenser stand installed outside a sterile workbench, a culture medium container placed on this dispenser stand, and a culture medium suction device connected to this culture medium container. It consists of a raising and sending means, and a dispensing pipe that extends from the culture medium suction and sending means, passes through the side wall of the sterile workbench, and is connected to a culture container in the sterile workbench.

More specifically, a hood switch for operating the drive unit of the medium suction and delivery means is provided at the bottom of the sterile workbench.

[Operation] The operation of the above technical means will be explained with reference to FIG. 2.

FIG. 2 is a principle explanatory diagram showing the medium dispensing operation according to the present invention.

As shown in FIG. 2, a foldable dispenser stand 6 is provided outside the sterile workbench, and a culture medium container equipped with a medium suction and delivery means (details not shown) is provided on the dispensing table 6. 8, that is, the dispenser 20 is placed. A dispensing pipe 11 is connected from the culture medium suction and delivery means to the culture container 10 placed on the table 4 of the sterile work bench through the side wall 5 of the sterile work bench.

By stepping on the food switch 12, the medium suction and delivery means is activated, and the medium 9 outside the sterile workbench is dispensed through the dispensing pipe 11 into the culture container 10 inside the sterile workbench.

[Embodiment] An embodiment of the present invention will be described below with reference to FIGS. 1 to 4.

FIG. 1 is a schematic perspective view of a sterile workbench to which the medium dispensing device of the present invention is applied, and FIG. 3 is an operational explanation showing the state of medium suction in the medium dispensing device according to an embodiment of the present invention. FIG. 4 is an explanatory view of the operation of the apparatus shown in FIG. 3, showing a medium feeding state.

First, the configuration of the sterile workbench will be explained with reference to FIG.

The sterile workbench 1 shown in Fig. 1 is surrounded by side walls on three sides and one side is open to the operator, and is equipped with a high-performance filter 2 at the back of the upper part and an air suction port 3 at the back of the lower part. Work can be done cleanly on a table 4 in a sterile workbench where airflow circulates.

On the outside of this sterile workbench, under the side wall 5 and in an extension of the table 4, a dispenser stand 6 is provided so as to be foldable with a hinge (not shown) or the like.

A hole 7 for piping is bored in the side wall 5.

In this sterile workbench 1, a culture container 10 is placed on a table 4, as shown in FIG. In the figure, only one is shown, but in reality, the required number can be placed.

A culture medium 9 is stored on the dispenser table 6 outside the sterile work table 1,
A culture medium container 8 equipped with a culture medium suction and delivery means (details not shown in FIG. 2) is placed. Then, the dispensing pipe 11 is passed from the culture medium suction and delivery means to the piping hole 7 of the side wall 5.
is connected to the culture container 10 on the table 4.

An embodiment of the medium suction and delivery means will be described with reference to FIGS. 3 and 4.

In Figures 3 and 4, 13 is the motor, 14 is the motor↓
15 is a syringe that moves vertically within the bilulet; 16 is a suction tube that sucks up the medium 9 in the medium container 8; 17 is a Billet part connected to suction pipe 16, work 8
19 is a suction on-off valve provided at the connection between the suction pipe 16 and the billet portion 17;
This is a delivery opening/closing valve provided at the connection between the pipe and the dispensing pipe 11. These constitute a dispenser 20 placed on the dispenser stand 6.

The operation of the dispenser 20 is performed by the food switch 2 installed at the bottom of the sterile workbench 1.
2 is electrically connected to the motor 13.

When performing dispensing work, connect the mass dispensing pipe 11 to the culture container 1.
Connect to 0. In order to move the dispensing pipe 11 to a desired position through the piping hole 7, the dispensing pipe 11 is preferably a flexible pipe.

The dispensing operation is performed by moving the syringe 15 in the billet section 17 in the vertical direction by the motor 13, and the operation is performed by stepping on the food switch 12.

As shown in FIG. 3, when the motor 13 is rotated in the forward direction, the syringe 15 moves upward via the rotational force transmission section 14, and the bilulet section 17 is brought into a near-vacuum state. is opened, the delivery on/off valve 19 is closed, and the culture medium 9 is sucked from the suction pipe 16 to the billet part F.

Next, as shown in FIG. 4, when the motor 13 is rotated in the opposite direction, the syringe 15 moves downward, and the suction on-off valve 1
8 closes, the delivery on-off valve 19 opens, and the billet part 17'
The volume of the biulet part is compressed as shown in FIG.

According to this embodiment, (1) activating the food switch 12 by stepping on it frees up both hands and improves the efficiency of the medium dispensing work.

7-- (2) By placing the dispenser 20 on the dispenser stand 6 outside the sterile workbench 1, the work space of the table 4 can be used widely.

In addition, although the above-mentioned Example explained operation by a hood switch, this invention is not limited to this, For example, remote control is also possible. Furthermore, although an example has been described in which the culture medium suction and delivery operation of the dispenser is performed by motor drive, the present invention does not preclude the adoption of other simple dropper systems.

[Effects of the Invention] As described above in detail, the present invention provides a culture medium that makes the working space on a sterile workbench effective, allows workers to work without any inconvenience, and improves the efficiency of culture medium dispensing work. A dispensing device can be provided.

[Brief explanation of drawings]

Fig. 2 is a schematic perspective view of a sterile workbench to which the medium dispensing device of the present invention is applied; An operation explanatory diagram showing a culture medium suction state in a culture medium dispensing device according to an embodiment,
FIG. 4 is an explanatory diagram of the operation of the apparatus shown in FIG. 3, showing a medium feeding state. 1... Sterile workbench, 4... Table, 5... Side wall, 6... Dispenser stand, 7... Piping hole, 8... Culture medium container, 9... Culture medium , lO...Culture container, 11...
・Dispensing pipe, 12... Food switch, 13...
Motor, 15... Syringe, 17.17'... Billet part, 18... Suction on/off valve, 19... Outlet on/off valve, 20... Dispenser.

Claims (1)

[Scope of Claims] 1. A culture medium dispensing device for dispensing a culture medium from outside the sterile workbench into a culture container placed in a sterile workbench through which a clean airflow flows, comprising a folding device provided outside the sterile workbench. a dispensing device stand that can be used, a culture medium container to be placed on the dispensing device stand, a medium suction and delivery means connected to this medium container, and a medium suction and delivery means that penetrates the side wall of the sterile workbench. and a dispensing pipe connected to the culture container in the sterile workbench. 2. The culture medium dispensing device according to claim 1, further comprising a food switch provided at the bottom of the sterile work table for operating the drive unit of the culture medium suction and delivery means.