JPH024579B2 - - Google Patents

Description

[Detailed description of the invention]

The present invention relates to a drug that inactivates viruses in a human or animal body. During use, cell damage and other harmful side effects in the treated organism must be avoided. The feature of the present invention is that black pepper oil (Oleum Piperis nigri) and/or cinnamon flower oil (Oleum Grassiae Flores) (hereinafter also referred to as extracted oil) which can be obtained from aromatic plants by steam distillation are extracted by the weight of a living body. 5 to 500 mg for 50 kg, especially 25
It consists in being used in daily dosages of 100 mg to 100 mg. These extracted oils have a virucidal effect, that is, a virus-damaging effect, at concentrations that are 1/10 to 10 times lower than the concentration at which these extracted oils exhibit toxic effects on living cells. This wide spacing, as shown, presents an advantageous tolerance for dosing and thus allows for risk-free dosing in veterinary and human medicine. These extracted oils can be obtained from aromatic plants that have been used for many years in animal husbandry and human cultivation, and therefore have been proven to be harmless in the administration of concern. The amount of extracted oil used according to the invention does not cause any dangerous or harmful side effects. Black pepper oils that have proven suitable can be used, each alone or in admixture with one another. The pharmaceutical preparations or agents according to the invention, ie agents for inactivating viruses in the human or animal body, contain one or more of the extracted oils. Obtained from aromatic plant parts by steam distillation and then mixed into a pharmaceutically applicable carrier material in a weight ratio of 1:100 to 5:100.
It is manufactured by mixing in. The medicament according to the present invention, ie, the medicament for inactivating viruses in the human or animal body, consists of the extracted oil mixed in a pharmaceutically applicable carrier material in a mixing ratio of 1:100 to 5:100. Consisting of one or more. The extracted oils used can be obtained from aromatic plants by steam distillation as follows: Black pepper oil from black pepper berries; Cinnamon oil from laurel flowers. Instead of these natural black pepper oil and cinnamon oil, the same synthetic extracted oils can also be used, if available. However, natural extracted oils obtained from perfume plants are advantageous. The effects achieved by the present invention were demonstrated by the following comparative tests. Type “Grirardi Heart” (GH) (ATCC
CCL 27) “Flow 12000” (FL) (Flow Laboratories
GmbH02−150), “Intestine407” (IN) (ATCC
CCL6) and “Vero kindney” (VE) (Flow
The cell culture medium was grown under optimal culture conditions in different culture vessels, thus forming a carpet of cell culture medium containing approximately 0.25 mg of cell material each on the bottom of the vessel. was formed. Additionally, a suspension of viral particles of the virus Adeno Type 6 was added. From each cell type for a total of eight extract oils of the present invention and comparative extract oils listed in the table below.
Fourteen cell culture media were produced. Fourteen cell cultures of each cell type were treated with different amounts of the extracted oil and as follows. Both initial cell cultures maintained ¹⁰⁵ mg of extracted oil per 10 Kg of cell material. Both cell culture media maintained 10 ⁴ mg of extracted oil for 10 Kg of cell material. Both of the following cell culture media maintain 10 ³ mg of extracted oil per 10 Kg of cell material, and immediately up to the last of these 14 cell culture media, extract oil per 10 Kg of cell material.
The dose was maintained at 0.1 mg. Therefore, always two homogeneous cell cultures were treated with the same amount of the same extraction oil. One of these homogeneous cell cultures was left intact for control purposes, while the second one was further prepared with 0.25% cell material in the form of a viral suspension.
It was inoculated with 5×10 ⁶ virus particles per mg.
Other cell culture media and other extraction oils were also manipulated in a corresponding manner. The cell culture medium thus treated was left to stand and observed after 4 to 6 days. Observations were made by microscopic examination of the cell culture medium for cell damage. The observed lesions were classified into four stages as follows: stage 0 means no damage, stage 1 means relaxed growth of multinucleated bodies, and stage 2 when cells become rounded and grow from the bottom. Stage 3 means that the cell tissue has been significantly or completely destroyed. Cell cultures inoculated with viruses protected with very little extracted oil were shown to have reached stage 3 or 2 as the virus damaged the cells. Cell culture media inoculated with viruses containing very high extracted oil content also reached stage 3 or 2 because the cells were damaged by excess extracted oil. However, medium sized cell cultures showed stage 0 and thus no damage. Therefore, the medium-sized extracted oil content sufficiently damaged the virus and protected the cells against viral morbidity, yet the cells were not directly damaged by the extracted oil.
As mentioned above, in the cell culture medium inoculated with the virus, stage 0 was observed after 4 to 6 days, and stage 1 was only rarely observed.The extracted oil concentration was sufficient without damaging the cells. This results in significant viral damage, which is noted in the table below. The extracted oil used is listed in the first column of the table below, the abbreviated name of the cell material treated as described above is listed in the second column, and the essential oil is listed in the third column. 10 of the treated cellular material for the concentration interval at which no target cell damage is observed (thus stage 0).
The amount of extracted oil (in mg) used per kg is stated. This interval represents in each case a range of therapeutic effects extending over several powers of 10. Moreover, the desired virucidal action of all extracted oils listed in the table is achieved at concentrations that are several powers of 10 below the lowest concentration at which cell damage is observed and, for example, the microorganisms to be protected are damaged. It's already happening. For comparison, all cells without the addition of extract oil showed stage 3.
Furthermore, as a comparative example, similar tests were conducted on black pepper oil, cinnamon oil, cinnamon oil obtained by steam distillation from aromatic plants, linalyl acetate, cinnamaldehyde, safrole, carvone, and cis/trans-citral. List what has been implemented.

[Table]

[Example 1 (injection solution)]

Dissolve 50 g of black pepper oil in 1,2-propanediol 2. The solution was sterilized in an autoclave at 121°C for 50 minutes, then cooled and
Divide into 2g portions and transfer into ampoules. One ampoule contains 50 mg of black pepper oil;
and contains an average daily dosage for an adult weighing 70 kg for the treatment and prevention of epidemic cold infections. For human or animal patients of other weights:
The daily dosage must be varied in proportion to the patient's weight. The mixing ratio of black pepper oil to 1,2-propanediol used is in this example 2.5:100;
Other mixing ratios are possible for injection solutions, but also in the range 1:100 to 5:100.
In this case, the daily dosage of the injection solution must be adapted to the changed terpene content of the injection solution. Example 2 (Aerosol) 325 g of black pepper oil are dissolved in 631.8 g of ether mixed with 1805.07 g of ethanol. 31.6 g of ester of castor oil fatty acid and oxyethylated glycerin and 210.6 g of caprylic/capric triglyceride are mixed into this solution. this mixture
2.68 g are transferred together with 2.527 g of difluorodichloromethane as propellant into a jet container with a capacity of 20 cm ² . The injection vessel is closed and has a dosing valve that releases on each actuation a predetermined portion of the mixture under pressure of difluorodichloromethane to be injected as an aerosol. By correspondingly adjusting and measuring the dosing valve, each actuation releases an individual dose with 6.5 mg of black pepper oil. Aerosols are sprayed into the mouth or nose and inhaled to treat and prevent epidemic cold infections.
8 injection parts per day for an adult weighing 70 kg
Advantageous treatment is carried out with 8×6.5=50 mg of black pepper oil in total. Virus-infected skin areas can also be treated with aerosol, for which a skin area of 50 cm ² is injected in 7 injection areas with up to 6.5 mg of black pepper oil each. The mixing ratio of black pepper oil to the aerosol substance used may range from 12:100 to 20:100 in this example; Must be adapted to the soybean oil content. Example 3 (Capsule) A capsule filling is prepared from a mixture of 12.5 g of black pepper oil, 12.5 g of cinnamon oil and 3 g of soybean lecithin as an emulsifier. Each capsule contains 28 mg of this capsule fill and is surrounded by a capsule envelope consisting of 87.5 mg gelatin and 37.5 mg glycerin. For the treatment and prevention of epidemic cold infections, one to four capsules per day are administered orally to adult patients weighing 70 kg, with many capsules being distributed over the entire day. Administer. One capsule contains 25 mg of black pepper oil;
It can also be modified to contain 10 to 50 mg, but in this case the daily dosage has to be adapted to the modified black pepper oil content. Example 4 (Static) 1 g of black pepper oil is mixed into a form-stable carrier material. The carrier material consisted of 59.84 g petrolatum album and 39.16 g paraffin and was thoroughly mixed with black pepper oil at 70° C., subsequently cast into stakes in a mold and hardened by cooling. For topical treatment, the carrier material of the stake is abraded by friction and distributed onto the skin, thus in the example 1 ml of the stake material containing 5 mg of black pepper oil is distributed onto 50 cm ² of skin. This can be repeated three times a day. The mixing ratio of black pepper oil to carrier material used is 1:100 in this example; other mixing ratios and even mixing ratios in the range from 1:100 to 5:100 are possible. [Example 5 (Ointment)] Parahuinum durum 3.2g and white petrolatum
Heat 86.8g to 60°C and stir. Mix 10g cinnamon oil into the warm mixture. Cool the mixture;
and can be used for topical use as an ointment. Ointment containing approximately 5 mg of cinnamon oil
Dispense 0.1 ml onto 50 cm ² of skin surface. Do this 8 times a day
Can be repeated multiple times. In this example, the mixture ratio of cinnamon oil, Parahuinum durum and white petrolatum used is
11:100; other mixing ratios for the ointment are also possible, and in the range from 5:100 to 20:100. Example 6 (Ointment) A vapor-tight plaster foil is produced from a textile fabric which has been made vapor-tight by coating it with a synthetic resin on the underside. On the other side (contact side) the plaster is 1
It was covered with plaster to a thickness of mm. 97g of lead plaster, 9g of yellow wax, gum dammara to produce plaster.
9 g of colophonium, 10 g of colophonium and 1 g of turpentine are mixed, heated to 100° C. and stirred until the melt no longer foams. Next, 5 g of black pepper oil is stirred in, and then plaster is applied on the contact side of the plaster foil and hardened by cooling. Place the plaster on the skin surface with the contact side and leave it for 4 hours. In this case, it can subsequently be replenished with a new plaster. The mixing ratio of black pepper oil to plaster used is in this example 4:100; other mixing ratios are also possible, and even in the range of 1:100 to 10:100. With the formulations and treatments described in the examples, viral morbidity is prevented or suppressed, without having to endure cell damage or other serious side effects in the treated organism. In the above examples, no difference in effect is observed even if the black pepper oil or cinnamon oil used is replaced with each other or with a mixture thereof. In all these cases, a virucidal effect can be achieved, and in this case it is not necessary to endure undesirable side effects.

Claims (1)

[Claims] 1. A method for inactivating viruses in human or animal bodies, characterized by containing at least one of black pepper oil and cinnamon oil obtained from aromatic plants by steam distillation as a main component. Drugs that do. 2. The drug according to claim 1, characterized in that the pharmaceutically applicable carrier material contains at least one of black pepper oil and cinnamon oil. 3. Claim 1, which is in the form of an injection solution in which at least one of black pepper oil and cinnamon oil is dissolved in 1,2-propanediol in a weight ratio of 1:100 to 5:100. The drug according to Section 1 or Section 2. 4. At least one of black pepper oil and cinnamon oil is contained in the aerosol material in a weight ratio of 5:100 to 20:100, and the aerosol material contains 1 part of ether, 2 to 5 parts of ethanol, and castor oil. Esters of fatty acids and oxyethylated glycerin
0.02 to 0.1 part of caprylic/capric triglyceride and 0.2 to 1 part of caprylic/capric triglyceride, in the form of an aerosol under a propellant pressure of 2 to 6 parts of difluorodichloromethane. The drug described in Section 2. 5. The capsule has a capsule covering that completely surrounds the capsule filling, and the capsule filling consists of at least 10 to 50 mg of black pepper oil or cinnamon oil mixed with 1 to 8 mg of soybean lecithin as an emulsifier; The drug according to claim 1 or 2, which is in the form of a capsule and has a total weight of 80 to 200 mg. 6. Consisting of a stick-like shape-stable but abrasive carrier material containing at least one of black pepper oil and cinnamon oil in a weight ratio of 1:100 to 5:100, and the carrier material is 1:100 to 5:100. 3. The drug according to claim 1 or 2, which is in the form of a stick consisting of petrolatum album and paraffin in a mixing ratio of 1:1 to 2:1. 7. At least one of black pepper oil and cinnamon oil is mixed in the ointment carrier material in a weight ratio of 5:100 to 20:100, and the ointment carrier material is mixed in a weight ratio of 1:100 to 20:100.
The drug according to claim 1 or 2, characterized in that it is in the form of an ointment consisting of paraffin durum mixed with white petrolatum in a mixing ratio of 20 to 1:35. 8 On the contact side, there is a steam-tight ointment foil coated with an ointment substance with a thickness of 0.5 to 2 m/m, and the ointment substance contains at least one of black pepper oil and cinnamon oil in a weight ratio of 1 :100 to 10:100 with a carrier material, and the carrier material is 90 parts of single lead ointment,
Yellow wax 7-14 parts, Dammala gum 7-14 parts, colophonium 8-16 parts, and turpentine 0.5-3
3. The drug according to claim 1 or 2, which is in the form of an ointment.