JPH0224467B2 - - Google Patents

Info

Publication number
JPH0224467B2
JPH0224467B2 JP58006748A JP674883A JPH0224467B2 JP H0224467 B2 JPH0224467 B2 JP H0224467B2 JP 58006748 A JP58006748 A JP 58006748A JP 674883 A JP674883 A JP 674883A JP H0224467 B2 JPH0224467 B2 JP H0224467B2
Authority
JP
Japan
Prior art keywords
solution
azure
eosin
dye
methylene blue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP58006748A
Other languages
English (en)
Japanese (ja)
Other versions
JPS58134155A (ja
Inventor
Ee Herufuritsuchi Erein
Fuai Itsupu Kin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Miles Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Inc filed Critical Miles Inc
Publication of JPS58134155A publication Critical patent/JPS58134155A/ja
Publication of JPH0224467B2 publication Critical patent/JPH0224467B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
    • C09B67/0033—Blends of pigments; Mixtured crystals; Solid solutions
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
    • C09B67/0071—Process features in the making of dyestuff preparations; Dehydrating agents; Dispersing agents; Dustfree compositions
    • C09B67/0083—Solutions of dyes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
JP58006748A 1982-02-01 1983-01-20 安定化されたロマノウスキイ型染色溶液 Granted JPS58134155A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/344,390 US4392864A (en) 1982-02-01 1982-02-01 Stabilized Romanowsky stain solution
US344390 1994-11-23

Publications (2)

Publication Number Publication Date
JPS58134155A JPS58134155A (ja) 1983-08-10
JPH0224467B2 true JPH0224467B2 (enExample) 1990-05-29

Family

ID=23350351

Family Applications (1)

Application Number Title Priority Date Filing Date
JP58006748A Granted JPS58134155A (ja) 1982-02-01 1983-01-20 安定化されたロマノウスキイ型染色溶液

Country Status (6)

Country Link
US (1) US4392864A (enExample)
EP (1) EP0086951B1 (enExample)
JP (1) JPS58134155A (enExample)
AU (1) AU532581B2 (enExample)
CA (1) CA1167749A (enExample)
DE (1) DE3361084D1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU186309B (en) * 1983-09-02 1985-07-29 Reanal Finomvegyszergyar Reagent for determining the thrombacyta and leucocyte number
US4595524A (en) * 1984-03-28 1986-06-17 Miles Laboratories, Inc. Two component stain composition for producing a Giemsa blood stain effect
US4741898A (en) * 1985-04-01 1988-05-03 Fisher Scientific Company Stabilized stain composition
DE3533515A1 (de) * 1985-09-20 1987-04-02 Merck Patent Gmbh Stabilisator fuer faerbeloesungen
US5175109A (en) * 1986-09-10 1992-12-29 Toa Medical Electronics Co., Ltd. Reagent for classifying leukocytes by flow cytometry
US5039613A (en) * 1986-11-27 1991-08-13 Toa Medical Electronics Co., Ltd. Reagents used in a method of classifying leukocytes by flow cytometry
US5179026A (en) * 1986-11-27 1993-01-12 Toa Medical Electronics Co., Ltd. Method of classifying leukocytes by flow cytometry and reagents used in the method
DE3736490A1 (de) * 1987-10-28 1989-05-11 Merck Patent Gmbh Stabilisatoren fuer faerbeloesungen
US6241788B1 (en) * 1999-11-16 2001-06-05 Betzdearborn Inc. Method of stabilizing dye solutions and stabilized dye compositions
US6858432B2 (en) * 2002-08-29 2005-02-22 Wescor, Inc. Method and staining reagent for staining hematology sample in an automated staining apparatus
FR2854239B1 (fr) * 2003-04-23 2006-03-10 Reactifs Ral Procede de preparation d'un reactif de coloration de cellules et reactif obtenu
CN111610138B (zh) * 2011-04-15 2023-06-09 罗氏血液诊断股份有限公司 测量细胞体积和成份
EP2721391B1 (en) 2011-06-17 2022-04-06 Roche Diagnostics Hematology, Inc. Solution and method for histoprocessing of biological samples
CN102980793B (zh) * 2012-11-20 2015-05-27 武汉友芝友生物制药有限公司 循环肿瘤细胞染色试剂盒及其应用
EP2954301B1 (en) * 2013-02-05 2024-06-12 Tripath Imaging, Inc. Cytological staining compositions and uses thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1183499A (en) * 1966-12-05 1970-03-04 Baxter Laboratories Inc Biological Stains
US4290769A (en) * 1980-10-14 1981-09-22 Miles Laboratories, Inc. Stabilized Romanowsky stain solution
US4382075A (en) * 1981-04-03 1983-05-03 Miles Laboratories, Inc. Stabilized Romanowsky stain solution
US4363632A (en) * 1981-12-28 1982-12-14 Miles Laboratories, Inc. Stabilized solution producing a Romanowsky effect

Also Published As

Publication number Publication date
EP0086951B1 (en) 1985-10-30
AU8784082A (en) 1983-08-11
EP0086951A1 (en) 1983-08-31
AU532581B2 (en) 1983-10-06
US4392864A (en) 1983-07-12
DE3361084D1 (en) 1985-12-05
JPS58134155A (ja) 1983-08-10
CA1167749A (en) 1984-05-22

Similar Documents

Publication Publication Date Title
JPH0224467B2 (enExample)
Tsien et al. [14] Measurement of cytosolic free Ca2+ with quin2
Enerbäck Berberine sulphate binding to mast cell polyanions: a cytofluorometric method for the quantitation of heparin
US6329205B1 (en) Detection method using luminescent europium-based protein stains
Araki et al. Accurate determination of DNA content in single cell nuclei stained with Hoechst 33258 fluorochrome at high salt concentration
WO2009003948A2 (de) Verfahren zur verbesserung der photostabilität und/oder kontrolle der fluoreszenzintensität von fluoreszenzfarbstoffen
US4018530A (en) Fluorescence spectrometry employing excitation of bleaching intensity
Godman et al. The composition of the LE and hematoxylin bodies of systemic lupus erythematosus
JP6475671B2 (ja) タンパク質検出
JP6950922B2 (ja) 染色方法、染色剤、及び染色キット
Tas et al. The Naphthol Yellow S stain for proteins tested in a model system of polyacrylamide films and evaluated for practical use in histochemistry
EP1619256B1 (en) Microorganism staining agent and use thereof
Jakovljevic Lead or thallium salts as external heavy atoms for room temperature quantitative phosphorescence
MAGUN et al. A new fluorescent method with phenanthrenequinone for the histochemical demonstration of arginine residues in tissues
JPH0129264B2 (enExample)
JP2005156564A (ja) 濃縮アゾ染色液、これを含む染色用キット、及びタンパク質染色液の調製方法
Blaies et al. A simplified method for staining mast cells with astra blue
VIALLI et al. Contribution to the cytospectrofluorometric measurement of 5-hydroxytryptamine in enterochromaffin cells
Bottiroli et al. Propidium iodide and the thiol‐specific reagent dacm as a dye pair for fluorescence resonance energy transfer analysis: An application to mouse sperm chromatin
US20040089847A1 (en) Stain solution which is devoid of phenol
EP0243300A2 (de) Nachweis von Pilzen
JPH07113807A (ja) リチウム測定のための試薬組成物と方法
US4137299A (en) Biological staining composition and staining method
CN117624107B (zh) 线粒体动态sted成像的罗丹明荧光探针及其制备方法
JP2014044119A (ja) 電子顕微鏡観察用染色剤および電子顕微鏡観察用試料の染色方法