JPH0220235B2 - - Google Patents
Info
- Publication number
- JPH0220235B2 JPH0220235B2 JP57119000A JP11900082A JPH0220235B2 JP H0220235 B2 JPH0220235 B2 JP H0220235B2 JP 57119000 A JP57119000 A JP 57119000A JP 11900082 A JP11900082 A JP 11900082A JP H0220235 B2 JPH0220235 B2 JP H0220235B2
- Authority
- JP
- Japan
- Prior art keywords
- hukn
- adsorbent
- units
- purity
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000872 buffer Substances 0.000 claims description 32
- 210000002700 urine Anatomy 0.000 claims description 23
- 239000005995 Aluminium silicate Substances 0.000 claims description 15
- 235000012211 aluminium silicate Nutrition 0.000 claims description 15
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims description 15
- 229920001661 Chitosan Polymers 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 11
- 102000001399 Kallikrein Human genes 0.000 claims description 7
- 108060005987 Kallikrein Proteins 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 230000007935 neutral effect Effects 0.000 claims description 3
- 239000003463 adsorbent Substances 0.000 description 45
- 230000000694 effects Effects 0.000 description 28
- 238000011084 recovery Methods 0.000 description 28
- 102000004169 proteins and genes Human genes 0.000 description 27
- 108090000623 proteins and genes Proteins 0.000 description 27
- 239000000284 extract Substances 0.000 description 25
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 13
- 238000001914 filtration Methods 0.000 description 11
- VEXZGXHMUGYJMC-UHFFFAOYSA-N hydrochloric acid Substances Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 11
- 239000011780 sodium chloride Substances 0.000 description 10
- 238000003756 stirring Methods 0.000 description 10
- 239000008363 phosphate buffer Substances 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 7
- 238000000605 extraction Methods 0.000 description 7
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 5
- 238000010828 elution Methods 0.000 description 5
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- -1 PH9.0 Substances 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 229920005654 Sephadex Polymers 0.000 description 2
- 239000012507 Sephadex™ Substances 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- 239000008351 acetate buffer Substances 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 102000010631 Kininogens Human genes 0.000 description 1
- 108010077861 Kininogens Proteins 0.000 description 1
- 102000002397 Kinins Human genes 0.000 description 1
- 108010093008 Kinins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000010200 folin Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 150000003512 tertiary amines Chemical class 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 230000024883 vasodilation Effects 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57119000A JPS5911179A (ja) | 1982-07-07 | 1982-07-07 | 人尿カリクレインの抽出法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57119000A JPS5911179A (ja) | 1982-07-07 | 1982-07-07 | 人尿カリクレインの抽出法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5911179A JPS5911179A (ja) | 1984-01-20 |
| JPH0220235B2 true JPH0220235B2 (show.php) | 1990-05-08 |
Family
ID=14750513
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57119000A Granted JPS5911179A (ja) | 1982-07-07 | 1982-07-07 | 人尿カリクレインの抽出法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5911179A (show.php) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05163158A (ja) * | 1991-12-13 | 1993-06-29 | Sanwa Kagaku Kenkyusho Co Ltd | 人尿性キニノゲナーゼを有効成分とする皮膚潰瘍の予防及び治療剤 |
-
1982
- 1982-07-07 JP JP57119000A patent/JPS5911179A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5911179A (ja) | 1984-01-20 |
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