JPH021156B2 - - Google Patents
Info
- Publication number
- JPH021156B2 JPH021156B2 JP56022276A JP2227681A JPH021156B2 JP H021156 B2 JPH021156 B2 JP H021156B2 JP 56022276 A JP56022276 A JP 56022276A JP 2227681 A JP2227681 A JP 2227681A JP H021156 B2 JPH021156 B2 JP H021156B2
- Authority
- JP
- Japan
- Prior art keywords
- anticancer
- substance
- anticancer substance
- fraction
- absorption
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 77
- 230000001093 anti-cancer Effects 0.000 claims description 73
- 239000000126 substance Substances 0.000 claims description 66
- 239000002244 precipitate Substances 0.000 claims description 50
- 239000000243 solution Substances 0.000 claims description 49
- 238000011282 treatment Methods 0.000 claims description 29
- 239000006228 supernatant Substances 0.000 claims description 28
- 238000006243 chemical reaction Methods 0.000 claims description 24
- 238000000034 method Methods 0.000 claims description 23
- 150000003839 salts Chemical class 0.000 claims description 23
- 238000010521 absorption reaction Methods 0.000 claims description 22
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 18
- 239000003960 organic solvent Substances 0.000 claims description 17
- 238000004519 manufacturing process Methods 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 15
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 15
- 108091005804 Peptidases Proteins 0.000 claims description 14
- 238000000862 absorption spectrum Methods 0.000 claims description 13
- 230000002255 enzymatic effect Effects 0.000 claims description 13
- 239000000843 powder Substances 0.000 claims description 13
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 12
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 12
- 241000605909 Fusobacterium Species 0.000 claims description 12
- 238000012258 culturing Methods 0.000 claims description 12
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 10
- 239000007864 aqueous solution Substances 0.000 claims description 10
- 241000605986 Fusobacterium nucleatum Species 0.000 claims description 9
- 206010028980 Neoplasm Diseases 0.000 claims description 9
- 102000035195 Peptidases Human genes 0.000 claims description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- 201000011510 cancer Diseases 0.000 claims description 8
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 claims description 8
- 241000699670 Mus sp. Species 0.000 claims description 7
- 239000008103 glucose Substances 0.000 claims description 7
- 239000002904 solvent Substances 0.000 claims description 7
- 206010003445 Ascites Diseases 0.000 claims description 6
- 108010059712 Pronase Proteins 0.000 claims description 6
- 239000004365 Protease Substances 0.000 claims description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims description 6
- 201000009030 Carcinoma Diseases 0.000 claims description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 5
- 108090000631 Trypsin Proteins 0.000 claims description 5
- 102000004142 Trypsin Human genes 0.000 claims description 5
- 238000011481 absorbance measurement Methods 0.000 claims description 5
- 230000003308 immunostimulating effect Effects 0.000 claims description 5
- 239000012588 trypsin Substances 0.000 claims description 5
- MYKOKMFESWKQRX-UHFFFAOYSA-N 10h-anthracen-9-one;sulfuric acid Chemical compound OS(O)(=O)=O.C1=CC=C2C(=O)C3=CC=CC=C3CC2=C1 MYKOKMFESWKQRX-UHFFFAOYSA-N 0.000 claims description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 4
- 239000002246 antineoplastic agent Substances 0.000 claims description 4
- 229940098773 bovine serum albumin Drugs 0.000 claims description 4
- 238000000921 elemental analysis Methods 0.000 claims description 4
- RJTZUHVCZIGJMB-UHFFFAOYSA-N hydron;1h-indole;chloride Chemical compound Cl.C1=CC=C2NC=CC2=C1 RJTZUHVCZIGJMB-UHFFFAOYSA-N 0.000 claims description 4
- 238000002844 melting Methods 0.000 claims description 4
- 230000008018 melting Effects 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 239000003480 eluent Substances 0.000 claims description 3
- 229910052739 hydrogen Inorganic materials 0.000 claims description 3
- 150000002500 ions Chemical class 0.000 claims description 3
- FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical compound C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 claims description 3
- 239000008363 phosphate buffer Substances 0.000 claims description 3
- 238000002835 absorbance Methods 0.000 claims 1
- 238000005194 fractionation Methods 0.000 claims 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 claims 1
- 230000001568 sexual effect Effects 0.000 claims 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 20
- 102000004190 Enzymes Human genes 0.000 description 17
- 108090000790 Enzymes Proteins 0.000 description 17
- 229940088598 enzyme Drugs 0.000 description 17
- 238000005406 washing Methods 0.000 description 15
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 14
- 210000004027 cell Anatomy 0.000 description 13
- 238000005119 centrifugation Methods 0.000 description 10
- 238000003756 stirring Methods 0.000 description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 239000007924 injection Substances 0.000 description 8
- 238000002347 injection Methods 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 230000001580 bacterial effect Effects 0.000 description 7
- 239000001888 Peptone Substances 0.000 description 6
- 108010080698 Peptones Proteins 0.000 description 6
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 6
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 6
- 238000007796 conventional method Methods 0.000 description 6
- 238000001816 cooling Methods 0.000 description 6
- 235000019319 peptone Nutrition 0.000 description 6
- 239000002504 physiological saline solution Substances 0.000 description 6
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 6
- -1 ethanol and methanol Chemical class 0.000 description 5
- 239000012228 culture supernatant Substances 0.000 description 4
- 229910001873 dinitrogen Inorganic materials 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 238000010908 decantation Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000008101 lactose Substances 0.000 description 3
- 231100000252 nontoxic Toxicity 0.000 description 3
- 230000003000 nontoxic effect Effects 0.000 description 3
- 230000000144 pharmacologic effect Effects 0.000 description 3
- 235000019419 proteases Nutrition 0.000 description 3
- 235000010265 sodium sulphite Nutrition 0.000 description 3
- GNBVPFITFYNRCN-UHFFFAOYSA-M sodium thioglycolate Chemical compound [Na+].[O-]C(=O)CS GNBVPFITFYNRCN-UHFFFAOYSA-M 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 239000003610 charcoal Substances 0.000 description 2
- 229920001429 chelating resin Polymers 0.000 description 2
- 230000005757 colony formation Effects 0.000 description 2
- 239000012531 culture fluid Substances 0.000 description 2
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
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- 230000036737 immune function Effects 0.000 description 2
- 238000007918 intramuscular administration Methods 0.000 description 2
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- 229940066779 peptones Drugs 0.000 description 2
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- 229940046307 sodium thioglycolate Drugs 0.000 description 2
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- 238000007920 subcutaneous administration Methods 0.000 description 2
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- 241001148471 unidentified anaerobic bacterium Species 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 1
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- NNJVILVZKWQKPM-UHFFFAOYSA-N Lidocaine Chemical compound CCN(CC)CC(=O)NC1=C(C)C=CC=C1C NNJVILVZKWQKPM-UHFFFAOYSA-N 0.000 description 1
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- 230000003307 reticuloendothelial effect Effects 0.000 description 1
- 210000002235 sarcomere Anatomy 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000019086 sulfide ion homeostasis Effects 0.000 description 1
- 239000010414 supernatant solution Substances 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 229960001322 trypsin Drugs 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Compounds Of Unknown Constitution (AREA)
Priority Applications (22)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56022276A JPS57139088A (en) | 1981-02-19 | 1981-02-19 | Carcinostatic substance tf-340, its preparation and carcinostatic agent containing the same |
| US06/347,871 US4477437A (en) | 1981-02-19 | 1982-02-11 | Substances having carcinostatic and immunostimulating activity |
| GB8204093A GB2093347B (en) | 1981-02-19 | 1982-02-12 | Immunostimulant material tf-2 from fusobacterium sp |
| DE19823205074 DE3205074A1 (de) | 1981-02-19 | 1982-02-12 | Neue carcinostatische und immunostimulierende substanzen, verfahren zur herstellung derselben und carcinostatische mittel mit einem gehalt derselben |
| DD82237480A DD202891A5 (de) | 1981-02-19 | 1982-02-17 | Neue carcinostatische und immunostimulierende substanzen,verfahren zu ihrer herstellung und carcinostatische mittel mit einem gehalt derselben |
| CA000396425A CA1184864A (en) | 1981-02-19 | 1982-02-17 | Substances having carcinostatic and immunostimulating activity, process for preparing the same and carcinostatic agent containing the same |
| CH979/82A CH651052A5 (de) | 1981-02-19 | 1982-02-17 | Verfahren zur herstellung einer karzinostatischen substanz und karzinostatische substanz. |
| IT47817/82A IT1189224B (it) | 1981-02-19 | 1982-02-17 | Sostanze carcinostatiche ed immunostimolanti tf-2,procedimento per prepararle ed agente carcinostatico che le contiene |
| DK069282A DK151640C (da) | 1981-02-19 | 1982-02-17 | Fremgangsmaade til fremstilling af forbindelser med carcinostatisk og immunostimulerende virkning |
| FI820527A FI69096C (fi) | 1981-02-19 | 1982-02-17 | Foerfarande foer framstaellning av en karcinostatisk och immunostimulerande tf-2-substans |
| FR8202601A FR2499855B1 (fr) | 1981-02-19 | 1982-02-17 | Nouvelles substances a action carcinostatique et immuno-stimulante, procede pour les preparer a partir de bacteries du genre fusobacterium, et agent carcinostatique contenant de telles substances |
| SE8201016A SE457000B (sv) | 1981-02-19 | 1982-02-18 | Foerfarande foer framstaellning av en carcinostatisk och immunostimulerande tf-2-substans, carcinostatisk tf-2-substans samt carcinostatiskt medel. |
| NZ199771A NZ199771A (en) | 1981-02-19 | 1982-02-18 | Carcinostatic and immunostimulating substances produced by fusobacterium nucleatum |
| NL8200640A NL8200640A (nl) | 1981-02-19 | 1982-02-18 | Nieuwe, carcinostatische stoffen, preparaten die ze bevatten, en werkwijze voor de bereiding daarvan. |
| KR8200714A KR890002256B1 (ko) | 1981-02-19 | 1982-02-18 | 항암 물질 tf-2 제조 방법 |
| PT74455A PT74455B (en) | 1981-02-19 | 1982-02-18 | Process for preparing novel substances having carcinostatic and immunostimulating activity and of a carcinostatic agent containing same |
| NO820509A NO157426C (no) | 1981-02-19 | 1982-02-18 | Fremgangsmaate for fremstilling av terapeutisk aktive forbindelser ved dyrking av mikroorganismer. |
| ES509774A ES509774A0 (es) | 1981-02-19 | 1982-02-19 | "un procedimiento para la preparacion de una sustancia carcinostatica tf-2". |
| AT0064682A AT381722B (de) | 1981-02-19 | 1982-02-19 | Verfahren zur herstellung einer neuen carcinostatischen substanz |
| BE0/207355A BE892204A (fr) | 1981-02-19 | 1982-02-19 | Nouvelles substances a action carcinostatique et immunq-stimulante, procede pour les preparer a partir de bacteries du genre fusobacterium, et agent carcinostatique contenat de telles substances |
| US06/619,895 US4591558A (en) | 1981-02-19 | 1984-08-06 | Novel substances having antitumor and immunostimulating activity, process for preparing the same and antitumor agent containing the same |
| DK251186A DK165641C (da) | 1981-02-19 | 1986-05-29 | Fremgangsmaade til fremstilling af tf-220, tf-230 og tf-240 forbindelser og de tilsvarende proteinfrie forbindelser |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56022276A JPS57139088A (en) | 1981-02-19 | 1981-02-19 | Carcinostatic substance tf-340, its preparation and carcinostatic agent containing the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS57139088A JPS57139088A (en) | 1982-08-27 |
| JPH021156B2 true JPH021156B2 (it) | 1990-01-10 |
Family
ID=12078229
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP56022276A Granted JPS57139088A (en) | 1981-02-19 | 1981-02-19 | Carcinostatic substance tf-340, its preparation and carcinostatic agent containing the same |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS57139088A (it) |
-
1981
- 1981-02-19 JP JP56022276A patent/JPS57139088A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS57139088A (en) | 1982-08-27 |
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