JP7853958B2 - 組み換えａａｖの作製方法 - Google Patents

組み換えａａｖの作製方法

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Publication number
JP7853958B2
JP7853958B2 JP2023512464A JP2023512464A JP7853958B2 JP 7853958 B2 JP7853958 B2 JP 7853958B2 JP 2023512464 A JP2023512464 A JP 2023512464A JP 2023512464 A JP2023512464 A JP 2023512464A JP 7853958 B2 JP7853958 B2 JP 7853958B2
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Japan
Prior art keywords
aav
cells
recombinant
gene
host cell
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2023512464A
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English (en)
Japanese (ja)
Other versions
JP2023538409A (ja
JP2023538409A5 (enExample
Inventor
ケイウッド、ライアン
スー、ウェイヘン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford Genetics Ltd
Original Assignee
Oxford Genetics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Oxford Genetics Ltd filed Critical Oxford Genetics Ltd
Publication of JP2023538409A publication Critical patent/JP2023538409A/ja
Publication of JP2023538409A5 publication Critical patent/JP2023538409A5/ja
Priority to JP2026068682A priority Critical patent/JP2026123082A/ja
Application granted granted Critical
Publication of JP7853958B2 publication Critical patent/JP7853958B2/ja
Active legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10041—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10044—Chimeric viral vector comprising heterologous viral elements for production of another viral vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10344—Chimeric viral vector comprising heterologous viral elements for production of another viral vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C12N2750/14152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
    • C12N2830/006—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB tet repressible

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2023512464A 2020-08-21 2021-08-20 組み換えａａｖの作製方法 Active JP7853958B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2026068682A JP2026123082A (ja) 2020-08-21 2026-04-17 組み換えａａｖの作製方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB2013057.1A GB202013057D0 (en) 2020-08-21 2020-08-21 Method of making recombinant aavs
GB2013057.1 2020-08-21
PCT/GB2021/052162 WO2022038369A1 (en) 2020-08-21 2021-08-20 Method of making recombinant aavs

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2026068682A Division JP2026123082A (ja) 2020-08-21 2026-04-17 組み換えａａｖの作製方法

Publications (3)

Publication Number Publication Date
JP2023538409A JP2023538409A (ja) 2023-09-07
JP2023538409A5 JP2023538409A5 (enExample) 2024-08-21
JP7853958B2 true JP7853958B2 (ja) 2026-04-30

Family

ID=72660685

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2023512464A Active JP7853958B2 (ja) 2020-08-21 2021-08-20 組み換えａａｖの作製方法
JP2026068682A Pending JP2026123082A (ja) 2020-08-21 2026-04-17 組み換えａａｖの作製方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2026068682A Pending JP2026123082A (ja) 2020-08-21 2026-04-17 組み換えａａｖの作製方法

Country Status (10)

Country Link
US (1) US20240026378A1 (enExample)
EP (1) EP4200428B1 (enExample)
JP (2) JP7853958B2 (enExample)
KR (1) KR20230074485A (enExample)
CN (1) CN116419974A (enExample)
AU (1) AU2021327855A1 (enExample)
CA (1) CA3189844A1 (enExample)
GB (1) GB202013057D0 (enExample)
MX (1) MX2023001936A (enExample)
WO (1) WO2022038369A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110892064A (zh) 2017-07-25 2020-03-17 牛津遗传学有限公司 腺病毒载体
GB201901571D0 (en) 2019-02-05 2019-03-27 Oxford Genetics Ltd Inducible AAV sysyem
US12054738B2 (en) 2020-07-30 2024-08-06 Shape Therapeutics Inc. Stable cell lines for inducible production of rAAV virions
JP2024537896A (ja) 2021-10-18 2024-10-16 リジェネロン・ファーマシューティカルズ・インコーポレイテッド アデノウイルス随伴ウイルスポリヌクレオチドを含む真核細胞
AR127674A1 (es) 2021-10-18 2024-02-21 Regeneron Pharma Células de mamífero que comprenden genes cas9 integrados para producir sitios de integración estables, y células de mamífero que comprenden sitios de integración estables y otros sitios

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040180440A1 (en) 2002-08-28 2004-09-16 Sergei Zolotukhin Modified AAV
JP2004534543A (ja) 2001-07-13 2004-11-18 ユニバーシテイ・オブ・アイオワ・リサーチ・フアウンデーシヨン シュードタイプ化アデノ関連ウイルスおよびその使用
US20070015238A1 (en) 2002-06-05 2007-01-18 Snyder Richard O Production of pseudotyped recombinant AAV virions
WO2019020992A1 (en) 2017-07-25 2019-01-31 Oxford Genetics Limited ADENOVIRAL VECTOR

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8632764B2 (en) * 2008-04-30 2014-01-21 University Of North Carolina At Chapel Hill Directed evolution and in vivo panning of virus vectors
FR3069668B1 (fr) 2017-07-27 2021-02-12 Safran Procede et dispositif de recherche d'un defaut susceptible d'affecter un dispositif mecanique tournant de transmission de puissance

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004534543A (ja) 2001-07-13 2004-11-18 ユニバーシテイ・オブ・アイオワ・リサーチ・フアウンデーシヨン シュードタイプ化アデノ関連ウイルスおよびその使用
US20070015238A1 (en) 2002-06-05 2007-01-18 Snyder Richard O Production of pseudotyped recombinant AAV virions
US20040180440A1 (en) 2002-08-28 2004-09-16 Sergei Zolotukhin Modified AAV
WO2019020992A1 (en) 2017-07-25 2019-01-31 Oxford Genetics Limited ADENOVIRAL VECTOR
JP2020533956A (ja) 2017-07-25 2020-11-26 オックスフォード ジェネティクス リミテッドＯｘｆｏｒｄ Ｇｅｎｅｔｉｃｓ Ｌｉｍｉｔｅｄ アデノウイルスベクター

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JOURNAL OF VIROLOGY，2005年，Vol.79, No.1，pp.193-201，doi:10.1128/JVI.79.1.193_201.2005

Also Published As

Publication number Publication date
JP2026123082A (ja) 2026-07-29
CA3189844A1 (en) 2022-02-24
WO2022038369A1 (en) 2022-02-24
US20240026378A1 (en) 2024-01-25
AU2021327855A9 (en) 2023-07-13
KR20230074485A (ko) 2023-05-30
GB202013057D0 (en) 2020-10-07
EP4200428B1 (en) 2025-02-19
CN116419974A (zh) 2023-07-11
AU2021327855A1 (en) 2023-05-04
EP4200428A1 (en) 2023-06-28
JP2023538409A (ja) 2023-09-07
MX2023001936A (es) 2023-04-10

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