JP7853277B2 - スルホン化ｄｎａの精製 - Google Patents
スルホン化ｄｎａの精製Info
- Publication number
- JP7853277B2 JP7853277B2 JP2023505706A JP2023505706A JP7853277B2 JP 7853277 B2 JP7853277 B2 JP 7853277B2 JP 2023505706 A JP2023505706 A JP 2023505706A JP 2023505706 A JP2023505706 A JP 2023505706A JP 7853277 B2 JP7853277 B2 JP 7853277B2
- Authority
- JP
- Japan
- Prior art keywords
- dna
- solution
- acidic
- sulfonated
- binding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2523/00—Reactions characterised by treatment of reaction samples
- C12Q2523/10—Characterised by chemical treatment
- C12Q2523/125—Bisulfite(s)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/119—Reactions demanding special reaction conditions pH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/125—Specific component of sample, medium or buffer
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063058179P | 2020-07-29 | 2020-07-29 | |
| US63/058,179 | 2020-07-29 | ||
| PCT/US2021/043769 WO2022039904A2 (en) | 2020-07-29 | 2021-07-29 | Purification of sulfonated dna |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2023536085A JP2023536085A (ja) | 2023-08-23 |
| JP2023536085A5 JP2023536085A5 (https=) | 2024-08-05 |
| JP7853277B2 true JP7853277B2 (ja) | 2026-04-28 |
Family
ID=80323147
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023505706A Active JP7853277B2 (ja) | 2020-07-29 | 2021-07-29 | スルホン化ｄｎａの精製 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20240026341A1 (https=) |
| EP (1) | EP4189086B1 (https=) |
| JP (1) | JP7853277B2 (https=) |
| KR (1) | KR20230041000A (https=) |
| CN (1) | CN116157519A (https=) |
| AU (1) | AU2021329235A1 (https=) |
| CA (1) | CA3186515A1 (https=) |
| WO (1) | WO2022039904A2 (https=) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2816122A1 (en) | 2008-02-15 | 2014-12-24 | Mayo Foundation For Medical Education And Research | Detecting neoplasm from a stool sample |
| US8361720B2 (en) | 2010-11-15 | 2013-01-29 | Exact Sciences Corporation | Real time cleavage assay |
| CA2902916C (en) | 2013-03-14 | 2018-08-28 | Mayo Foundation For Medical Education And Research | Detecting neoplasm |
| CN120700143A (zh) | 2014-03-31 | 2025-09-26 | 梅奥医学教育和研究基金会 | 检测结直肠赘生物 |
| US10184154B2 (en) | 2014-09-26 | 2019-01-22 | Mayo Foundation For Medical Education And Research | Detecting cholangiocarcinoma |
| US10435755B2 (en) | 2015-03-27 | 2019-10-08 | Exact Sciences Development Company, Llc | Detecting esophageal disorders |
| CN114574585A (zh) | 2015-08-31 | 2022-06-03 | 梅约医药教育及研究基金会 | 检测胃肿瘤 |
| CN108350485A (zh) | 2015-10-30 | 2018-07-31 | 精密科学发展有限责任公司 | 血浆dna的多重扩增检测测定以及分离和检测 |
| CN109563546B (zh) | 2016-05-05 | 2022-09-09 | 精密科学发展有限责任公司 | 通过分析甲基化dna来检测肺肿瘤 |
| JP7277460B2 (ja) | 2017-11-30 | 2023-05-19 | マヨ ファウンデーション フォア メディカル エデュケーション アンド リサーチ | 乳癌の検出 |
| US10648025B2 (en) | 2017-12-13 | 2020-05-12 | Exact Sciences Development Company, Llc | Multiplex amplification detection assay II |
| CA3127329A1 (en) | 2019-01-24 | 2020-07-30 | Mayo Foundation For Medical Education And Research | Detecting endometrial cancer |
| US11702704B2 (en) | 2019-10-31 | 2023-07-18 | Mayo Foundation For Medical Education And Research | Detecting ovarian cancer |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004089195A (ja) | 2002-08-29 | 2004-03-25 | F Hoffmann La Roche Ag | 亜硫酸水素塩処理のための改善された方法 |
| JP2009519023A (ja) | 2005-12-14 | 2009-05-14 | エフ．ホフマン−ラ ロシュ アーゲー | 重亜硫酸処理のための新規方法 |
| WO2014120918A1 (en) | 2013-01-30 | 2014-08-07 | Exact Sciences Corporation | Treatment of a sample vessel |
| US20160265033A1 (en) | 2012-01-30 | 2016-09-15 | Exact Sciences Corporation | Modification of dna on magnetic beads |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4554088A (en) | 1983-05-12 | 1985-11-19 | Advanced Magnetics Inc. | Magnetic particles for use in separations |
| FI932866A0 (fi) | 1993-06-21 | 1993-06-21 | Labsystems Oy | Separeringsfoerfarande |
| EP1130397B1 (en) | 1993-02-01 | 2006-10-11 | Thermo Electron Oy | Equipment for determination of an analyte from a sample |
| US5648124A (en) | 1993-07-09 | 1997-07-15 | Seradyn, Inc. | Process for preparing magnetically responsive microparticles |
| JP3115501B2 (ja) | 1994-06-15 | 2000-12-11 | プレシジョン・システム・サイエンス株式会社 | 分注機を利用した磁性体の脱着制御方法及びこの方法によって処理される各種装置 |
| FI944937A0 (fi) | 1994-10-20 | 1994-10-20 | Labsystems Oy | Separeringsanordning |
| US5786146A (en) | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| US6027945A (en) | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
| WO1999040098A1 (de) | 1998-02-04 | 1999-08-12 | Merck Patent Gmbh | Verfahren zur isolierung und aufreinigung von nucleinsäuren |
| US6194562B1 (en) | 1998-04-22 | 2001-02-27 | Promega Corporation | Endotoxin reduction in nucleic acid purification |
| US7078224B1 (en) | 1999-05-14 | 2006-07-18 | Promega Corporation | Cell concentration and lysate clearance using paramagnetic particles |
| US6270970B1 (en) | 1999-05-14 | 2001-08-07 | Promega Corporation | Mixed-bed solid phase and its use in the isolation of nucleic acids |
| EP1394172A1 (en) | 2002-08-29 | 2004-03-03 | Boehringer Mannheim Gmbh | Improved method for bisulfite treatment |
| EP2016188B1 (en) * | 2006-04-24 | 2015-10-21 | Xiyu Jia | Methods for detection of methylated dna |
| US8361720B2 (en) | 2010-11-15 | 2013-01-29 | Exact Sciences Corporation | Real time cleavage assay |
| US8916344B2 (en) | 2010-11-15 | 2014-12-23 | Exact Sciences Corporation | Methylation assay |
| US8715937B2 (en) | 2010-11-15 | 2014-05-06 | Exact Sciences Corporation | Mutation detection assay |
| CN104450680A (zh) | 2011-05-12 | 2015-03-25 | 精密科学公司 | 核酸的分离 |
| WO2013181651A1 (en) | 2012-06-01 | 2013-12-05 | Omega Bio-Tek, Inc. | Selective nucleic acid fragment recovery |
| US9212392B2 (en) | 2012-09-25 | 2015-12-15 | Exact Sciences Corporation | Normalization of polymerase activity |
| CA2965500A1 (en) * | 2014-10-24 | 2016-04-28 | Abbott Molecular Inc. | Enrichment of small nucleic acids |
| WO2018017710A1 (en) | 2016-07-19 | 2018-01-25 | Exact Sciences Development Company, Llc | Nucleic acid control molecules from non-human organisms |
| US10648025B2 (en) * | 2017-12-13 | 2020-05-12 | Exact Sciences Development Company, Llc | Multiplex amplification detection assay II |
| CN109022417A (zh) | 2018-08-13 | 2018-12-18 | 益善生物技术股份有限公司 | 一种磁珠法核酸提取转化试剂盒及其使用方法 |
-
2021
- 2021-07-29 AU AU2021329235A patent/AU2021329235A1/en active Pending
- 2021-07-29 CA CA3186515A patent/CA3186515A1/en active Pending
- 2021-07-29 JP JP2023505706A patent/JP7853277B2/ja active Active
- 2021-07-29 WO PCT/US2021/043769 patent/WO2022039904A2/en not_active Ceased
- 2021-07-29 US US18/005,943 patent/US20240026341A1/en active Pending
- 2021-07-29 CN CN202180059268.0A patent/CN116157519A/zh active Pending
- 2021-07-29 KR KR1020237003158A patent/KR20230041000A/ko active Pending
- 2021-07-29 EP EP21858796.2A patent/EP4189086B1/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004089195A (ja) | 2002-08-29 | 2004-03-25 | F Hoffmann La Roche Ag | 亜硫酸水素塩処理のための改善された方法 |
| JP2009519023A (ja) | 2005-12-14 | 2009-05-14 | エフ．ホフマン−ラ ロシュ アーゲー | 重亜硫酸処理のための新規方法 |
| US20160265033A1 (en) | 2012-01-30 | 2016-09-15 | Exact Sciences Corporation | Modification of dna on magnetic beads |
| WO2014120918A1 (en) | 2013-01-30 | 2014-08-07 | Exact Sciences Corporation | Treatment of a sample vessel |
Non-Patent Citations (1)
| Title |
|---|
| PLoS ONE，2017年，Vol.12, No.5，e0176848 (pp.1-14) |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2021329235A1 (en) | 2023-02-23 |
| KR20230041000A (ko) | 2023-03-23 |
| CA3186515A1 (en) | 2022-02-24 |
| EP4189086A2 (en) | 2023-06-07 |
| WO2022039904A2 (en) | 2022-02-24 |
| JP2023536085A (ja) | 2023-08-23 |
| US20240026341A1 (en) | 2024-01-25 |
| WO2022039904A3 (en) | 2022-06-02 |
| EP4189086B1 (en) | 2026-03-11 |
| CN116157519A (zh) | 2023-05-23 |
| EP4189086A4 (en) | 2024-07-17 |
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