CN116157519A - 经磺化dna的纯化 - Google Patents

经磺化dna的纯化 Download PDF

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Publication number
CN116157519A
CN116157519A CN202180059268.0A CN202180059268A CN116157519A CN 116157519 A CN116157519 A CN 116157519A CN 202180059268 A CN202180059268 A CN 202180059268A CN 116157519 A CN116157519 A CN 116157519A
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CN
China
Prior art keywords
dna
sulfonated
acidic
solution
sulfonated dna
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Pending
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CN202180059268.0A
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English (en)
Chinese (zh)
Inventor
E·M·拉格兰
Z·加格拉特
M·J·多马尼科
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Exact Sciences Corp
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Exact Sciences Corp
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Publication of CN116157519A publication Critical patent/CN116157519A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
    • C12Q2523/125—Bisulfite(s)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/119—Reactions demanding special reaction conditions pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
CN202180059268.0A 2020-07-29 2021-07-29 经磺化dna的纯化 Pending CN116157519A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063058179P 2020-07-29 2020-07-29
US63/058,179 2020-07-29
PCT/US2021/043769 WO2022039904A2 (en) 2020-07-29 2021-07-29 Purification of sulfonated dna

Publications (1)

Publication Number Publication Date
CN116157519A true CN116157519A (zh) 2023-05-23

Family

ID=80323147

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180059268.0A Pending CN116157519A (zh) 2020-07-29 2021-07-29 经磺化dna的纯化

Country Status (8)

Country Link
US (1) US20240026341A1 (https=)
EP (1) EP4189086B1 (https=)
JP (1) JP7853277B2 (https=)
KR (1) KR20230041000A (https=)
CN (1) CN116157519A (https=)
AU (1) AU2021329235A1 (https=)
CA (1) CA3186515A1 (https=)
WO (1) WO2022039904A2 (https=)

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EP2816122A1 (en) 2008-02-15 2014-12-24 Mayo Foundation For Medical Education And Research Detecting neoplasm from a stool sample
US8361720B2 (en) 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
CA2902916C (en) 2013-03-14 2018-08-28 Mayo Foundation For Medical Education And Research Detecting neoplasm
CN120700143A (zh) 2014-03-31 2025-09-26 梅奥医学教育和研究基金会 检测结直肠赘生物
US10184154B2 (en) 2014-09-26 2019-01-22 Mayo Foundation For Medical Education And Research Detecting cholangiocarcinoma
US10435755B2 (en) 2015-03-27 2019-10-08 Exact Sciences Development Company, Llc Detecting esophageal disorders
CN114574585A (zh) 2015-08-31 2022-06-03 梅约医药教育及研究基金会 检测胃肿瘤
CN108350485A (zh) 2015-10-30 2018-07-31 精密科学发展有限责任公司 血浆dna的多重扩增检测测定以及分离和检测
CN109563546B (zh) 2016-05-05 2022-09-09 精密科学发展有限责任公司 通过分析甲基化dna来检测肺肿瘤
JP7277460B2 (ja) 2017-11-30 2023-05-19 マヨ ファウンデーション フォア メディカル エデュケーション アンド リサーチ 乳癌の検出
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
CA3127329A1 (en) 2019-01-24 2020-07-30 Mayo Foundation For Medical Education And Research Detecting endometrial cancer
US11702704B2 (en) 2019-10-31 2023-07-18 Mayo Foundation For Medical Education And Research Detecting ovarian cancer

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FI932866A0 (fi) 1993-06-21 1993-06-21 Labsystems Oy Separeringsfoerfarande
EP1130397B1 (en) 1993-02-01 2006-10-11 Thermo Electron Oy Equipment for determination of an analyte from a sample
US5648124A (en) 1993-07-09 1997-07-15 Seradyn, Inc. Process for preparing magnetically responsive microparticles
JP3115501B2 (ja) 1994-06-15 2000-12-11 プレシジョン・システム・サイエンス株式会社 分注機を利用した磁性体の脱着制御方法及びこの方法によって処理される各種装置
FI944937A0 (fi) 1994-10-20 1994-10-20 Labsystems Oy Separeringsanordning
US5786146A (en) 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US6027945A (en) 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
WO1999040098A1 (de) 1998-02-04 1999-08-12 Merck Patent Gmbh Verfahren zur isolierung und aufreinigung von nucleinsäuren
US6194562B1 (en) 1998-04-22 2001-02-27 Promega Corporation Endotoxin reduction in nucleic acid purification
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
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CA2631829C (en) 2005-12-14 2012-01-24 F. Hoffmann-La Roche Ag New method for bisulfite treatment
EP2016188B1 (en) * 2006-04-24 2015-10-21 Xiyu Jia Methods for detection of methylated dna
US8361720B2 (en) 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
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Also Published As

Publication number Publication date
AU2021329235A1 (en) 2023-02-23
JP7853277B2 (ja) 2026-04-28
KR20230041000A (ko) 2023-03-23
CA3186515A1 (en) 2022-02-24
EP4189086A2 (en) 2023-06-07
WO2022039904A2 (en) 2022-02-24
JP2023536085A (ja) 2023-08-23
US20240026341A1 (en) 2024-01-25
WO2022039904A3 (en) 2022-06-02
EP4189086B1 (en) 2026-03-11
EP4189086A4 (en) 2024-07-17

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