JP7808028B2 - 加水分解酵素を利用した微量物質の測定方法 - Google Patents

加水分解酵素を利用した微量物質の測定方法

Info

Publication number
JP7808028B2
JP7808028B2 JP2022515447A JP2022515447A JP7808028B2 JP 7808028 B2 JP7808028 B2 JP 7808028B2 JP 2022515447 A JP2022515447 A JP 2022515447A JP 2022515447 A JP2022515447 A JP 2022515447A JP 7808028 B2 JP7808028 B2 JP 7808028B2
Authority
JP
Japan
Prior art keywords
substrate
hydrolase
compound
label
amino
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2022515447A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2021210669A1 (https=
JPWO2021210669A5 (https=
Inventor
直哉 戸塚
陽介 鉞
智一 末永
久美 井上
隆広 伊藤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kikkoman Corp
Original Assignee
Kikkoman Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kikkoman Corp filed Critical Kikkoman Corp
Publication of JPWO2021210669A1 publication Critical patent/JPWO2021210669A1/ja
Publication of JPWO2021210669A5 publication Critical patent/JPWO2021210669A5/ja
Application granted granted Critical
Publication of JP7808028B2 publication Critical patent/JP7808028B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Electrochemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2022515447A 2020-04-16 2021-04-16 加水分解酵素を利用した微量物質の測定方法 Active JP7808028B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020073489 2020-04-16
JP2020073489 2020-04-16
PCT/JP2021/015693 WO2021210669A1 (ja) 2020-04-16 2021-04-16 加水分解酵素を利用した微量物質の測定方法

Publications (3)

Publication Number Publication Date
JPWO2021210669A1 JPWO2021210669A1 (https=) 2021-10-21
JPWO2021210669A5 JPWO2021210669A5 (https=) 2024-04-24
JP7808028B2 true JP7808028B2 (ja) 2026-01-28

Family

ID=78084928

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022515447A Active JP7808028B2 (ja) 2020-04-16 2021-04-16 加水分解酵素を利用した微量物質の測定方法

Country Status (3)

Country Link
EP (1) EP4137817A4 (https=)
JP (1) JP7808028B2 (https=)
WO (1) WO2021210669A1 (https=)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002542154A (ja) 1999-02-23 2002-12-10 ペンタファルム アクチェンゲゼルシャフト プロテアーゼ活性の電気化学的測定のためのオリゴペプチド誘導体
US20160091511A1 (en) 2014-09-26 2016-03-31 Abbott Point Of Care Inc. Single channel cartridge device for coagulation assays in fluid samples
WO2017141961A1 (ja) 2016-02-16 2017-08-24 生化学工業株式会社 フェニレンジアミン誘導体を用いた電気化学測定
JP2019184580A (ja) 2018-04-16 2019-10-24 キッコーマン株式会社 新規メディエータ

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63154957A (ja) * 1986-12-19 1988-06-28 Terumo Corp 膜被覆センサの製造方法
WO1995014931A1 (fr) 1993-11-22 1995-06-01 Seikagaku Corporation Procede de dosage d'une substance reagissant avec un reactif de limulus
US6849426B2 (en) 2001-06-28 2005-02-01 Cambrex Bio Science Walkersville, Inc. Methods and reagents for detecting endotoxin
CN103562724B (zh) 2011-02-28 2016-10-05 生化学工业株式会社 用于测量内毒素的试剂
WO2014092079A1 (ja) 2012-12-10 2014-06-19 生化学工業株式会社 新規組換えファクターｃ、その製造法、およびエンドトキシンの測定法
WO2015136370A2 (en) 2014-03-10 2015-09-17 3-D Matrix, Ltd. Sterilization and filtration of peptide compositions
WO2015137356A1 (ja) 2014-03-11 2015-09-17 大日本印刷株式会社 微生物夾雑物の濃度検出方法、電極チップおよびオリゴペプチド
JP2018072331A (ja) 2016-10-25 2018-05-10 大日本印刷株式会社 エンドトキシン検出用チップおよびエンドトキシン濃度の定量方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002542154A (ja) 1999-02-23 2002-12-10 ペンタファルム アクチェンゲゼルシャフト プロテアーゼ活性の電気化学的測定のためのオリゴペプチド誘導体
US20160091511A1 (en) 2014-09-26 2016-03-31 Abbott Point Of Care Inc. Single channel cartridge device for coagulation assays in fluid samples
WO2017141961A1 (ja) 2016-02-16 2017-08-24 生化学工業株式会社 フェニレンジアミン誘導体を用いた電気化学測定
JP2019184580A (ja) 2018-04-16 2019-10-24 キッコーマン株式会社 新規メディエータ

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SZUBARGA Alex et al，ELECTROCHEMICAL ACTIVITY DETERMINATION OF TRYPSIN-LIKE ENZYMES. IX. FACTOR X AND FACTOR Xa IN PLASMA AND WHOLE BLOOD.，THROMBOSIS RESEARCH，Vol. 36, No. 6，1984年，Pages. 647-650

Also Published As

Publication number Publication date
JPWO2021210669A1 (https=) 2021-10-21
EP4137817A1 (en) 2023-02-22
WO2021210669A1 (ja) 2021-10-21
EP4137817A4 (en) 2024-08-07

Similar Documents

Publication Publication Date Title
Zhang et al. Development and analytical application of an uric acid biosensor using an uricase-immobilized eggshell membrane
Wu et al. Biosensors for determination of glucose with glucose oxidase immobilized on an eggshell membrane
Park et al. Washing-and separation-free electrochemical detection of porphyromonas gingivalis in saliva for initial diagnosis of periodontitis
EP2211167A1 (en) Method and kit for measurement of endotoxin level
JPH0684952B2 (ja) 分析方法およびセンサ電極
BRPI0716908A2 (pt) Indicadores de esterilização, kit indicador de esterilização, processo para manufaturar o mesmo, processo de esterilização e processos para determinar a eficiência de esterilização.
Cao et al. A simple and general approach to assay protease activity with electrochemical technique
Kaneto A new tyrosinase biosensor based on covalent immobilization of enzyme on N-(3-aminopropyl) pyrrole polymer film
JPWO2007094354A1 (ja) ヘモグロビンＡ１ｃセンサ
Park et al. Wash-free amperometric Escherichia coli detection via rapid and specific proteolytic cleavage by its outer membrane OmpT
CN105601658B (zh) 一种能够区分生物硫醇的荧光探针的制备与应用
Zhang et al. A novel electrochemical method to determine α-amylase activity
Cheng et al. Peptide cleavage induced assembly enables highly sensitive electrochemiluminescence detection of protease activity
Wu et al. A biosensor with myrosinase and glucose oxidase bienzyme system for determination of glucosinolates in seeds of commonly consumed vegetables
Abd-Rabboh et al. Electrochemical assay of protease activities based on polycation/polyanion complex as substrate and polyion sensitive membrane electrode detection
JP7808028B2 (ja) 加水分解酵素を利用した微量物質の測定方法
Dai et al. Optical detection of polycations via polymer film-modified microtiter plates: response mechanism and bioanalytical applications
Dash et al. Structural and mechanistic insight into the inhibition of aspartic proteases by a slow-tight binding inhibitor from an extremophilic Bacillus sp.: correlation of the kinetic parameters with the inhibitor induced conformational changes
US5223404A (en) Composition for testing periodontal diseases
EP0325472B1 (en) Composition for testing periodontal diseases
Chauhan et al. Biosensors
KR0140216B1 (ko) 치주질환 검사용 조성물
Rernglit et al. Real-time electrochemical measurement of N-acetyl-ß-D-glucosaminidase activity, using redox-tagged N-acetyl-ß-D-glucosaminide hydrolysis as proof-of-principle
Herrmann Dyes in diagnostics
JP2662228B2 (ja) 歯周病原性菌検査薬

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20240415

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20240415

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20250513

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20250711

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250911

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20260106

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20260116

R150 Certificate of patent or registration of utility model

Ref document number: 7808028

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150