JP7805590B2 - 可逆的不死化細胞の製造方法 - Google Patents

可逆的不死化細胞の製造方法

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Publication number
JP7805590B2
JP7805590B2 JP2022560822A JP2022560822A JP7805590B2 JP 7805590 B2 JP7805590 B2 JP 7805590B2 JP 2022560822 A JP2022560822 A JP 2022560822A JP 2022560822 A JP2022560822 A JP 2022560822A JP 7805590 B2 JP7805590 B2 JP 7805590B2
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cells
cell
gene
vector
immortalized
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Japanese (ja)
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JPWO2022097716A1 (https=
Inventor
光雄 押村
寿晃 田畑
康宏 香月
愛海 宇野
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Trans Chromosomics Inc
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Trans Chromosomics Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/18011—Paramyxoviridae
    • C12N2760/18811—Sendai virus
    • C12N2760/18841—Use of virus, viral particle or viral elements as a vector
    • C12N2760/18843—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2022560822A 2020-11-06 2021-11-05 可逆的不死化細胞の製造方法 Active JP7805590B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025181712A JP2026012286A (ja) 2020-11-06 2025-10-28 可逆的不死化細胞の製造方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020186146 2020-11-06
JP2020186146 2020-11-06
PCT/JP2021/040757 WO2022097716A1 (ja) 2020-11-06 2021-11-05 可逆的不死化細胞の製造方法

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JP2025181712A Division JP2026012286A (ja) 2020-11-06 2025-10-28 可逆的不死化細胞の製造方法

Publications (2)

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JPWO2022097716A1 JPWO2022097716A1 (https=) 2022-05-12
JP7805590B2 true JP7805590B2 (ja) 2026-01-26

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JP2022560822A Active JP7805590B2 (ja) 2020-11-06 2021-11-05 可逆的不死化細胞の製造方法
JP2025181712A Pending JP2026012286A (ja) 2020-11-06 2025-10-28 可逆的不死化細胞の製造方法

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Country Link
US (1) US20230407268A1 (https=)
EP (1) EP4242317A4 (https=)
JP (2) JP7805590B2 (https=)
CN (1) CN116419968A (https=)
WO (1) WO2022097716A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4636087A1 (en) 2022-11-22 2025-10-22 Kagoshima University Anti-gpa33 antibody and utilization thereof
CN118109517A (zh) * 2024-02-04 2024-05-31 南京市第一医院 一种永生化人结直肠癌成纤维细胞系及其构建方法和应用
WO2026005077A1 (ja) * 2024-06-24 2026-01-02 株式会社Trans Chromosomics 可逆的不死化細胞から不死化解除細胞を製造する方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003514565A (ja) 1999-11-25 2003-04-22 ユニヴェルシテ・ドゥ・ジュネーブ 非分裂細胞を不死化する能力をもつベクター及び前記ベクターで不死化された細胞
JP2003174870A (ja) 2001-10-12 2003-06-24 Naoya Kobayashi 不死化骨髄間葉系幹細胞
JP2009291157A (ja) 2008-06-06 2009-12-17 Hidetoshi Yamashita 結膜上皮細胞株
WO2010008054A1 (ja) 2008-07-16 2010-01-21 ディナベック株式会社 染色体非組み込み型ウイルスベクターを用いてリプログラムされた細胞を製造する方法
WO2010084970A1 (ja) 2009-01-23 2010-07-29 国立大学法人大阪大学 標的細胞誘導用フィーダー細胞
JP2012525825A (ja) 2009-05-06 2012-10-25 サノフイ 可逆的に不死化された細胞及びそれに関連する方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL296832A (en) 2005-10-18 2022-11-01 Nat Jewish Health Process of preparing red blood cells using conditionally immortalized long-term hematopoietic stem cells and erythropoietin
US10975358B2 (en) 2013-09-24 2021-04-13 Id Pharma Co., Ltd. Method for improving efficiency of inducing pluripotent stem cell
HK1257561A1 (zh) 2015-11-05 2019-10-25 株式会社Cysay 永生化干细胞及其制作方法
JP2020186146A (ja) 2019-05-13 2020-11-19 キヤノン株式会社 ガラスの製造方法および光学素子の製造方法
JP7369343B2 (ja) * 2019-10-11 2023-10-26 国立研究開発法人農業・食品産業技術総合研究機構 豚感染ウイルスの製造方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003514565A (ja) 1999-11-25 2003-04-22 ユニヴェルシテ・ドゥ・ジュネーブ 非分裂細胞を不死化する能力をもつベクター及び前記ベクターで不死化された細胞
JP2003174870A (ja) 2001-10-12 2003-06-24 Naoya Kobayashi 不死化骨髄間葉系幹細胞
JP2009291157A (ja) 2008-06-06 2009-12-17 Hidetoshi Yamashita 結膜上皮細胞株
WO2010008054A1 (ja) 2008-07-16 2010-01-21 ディナベック株式会社 染色体非組み込み型ウイルスベクターを用いてリプログラムされた細胞を製造する方法
WO2010084970A1 (ja) 2009-01-23 2010-07-29 国立大学法人大阪大学 標的細胞誘導用フィーダー細胞
JP2012525825A (ja) 2009-05-06 2012-10-25 サノフイ 可逆的に不死化された細胞及びそれに関連する方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Biochemical and Biophysical Research Communications，2012年，Vol. 422，pp. 28-35
Journal of Andrology，2002年，Vol. 23, No. 6，pp. 854-869
PNAS，2011年，Vol. 108, No. 34，pp. 14234-14239，https://www.pnas.org/content/108/34/14234参照

Also Published As

Publication number Publication date
JP2026012286A (ja) 2026-01-23
CN116419968A (zh) 2023-07-11
WO2022097716A1 (ja) 2022-05-12
JPWO2022097716A1 (https=) 2022-05-12
EP4242317A1 (en) 2023-09-13
US20230407268A1 (en) 2023-12-21
EP4242317A4 (en) 2024-11-13

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