JP7797623B2 - ポリエチレンテレフタレートの酵素分解 - Google Patents

ポリエチレンテレフタレートの酵素分解

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Publication number
JP7797623B2
JP7797623B2 JP2024508603A JP2024508603A JP7797623B2 JP 7797623 B2 JP7797623 B2 JP 7797623B2 JP 2024508603 A JP2024508603 A JP 2024508603A JP 2024508603 A JP2024508603 A JP 2024508603A JP 7797623 B2 JP7797623 B2 JP 7797623B2
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Prior art keywords
amino acid
acid substitution
petase
bhr
substitution
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JP2024508603A
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English (en)
Japanese (ja)
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JP2024531226A (ja
Inventor
ヤン，ジエ
ジャン，シユン
ウー，キン
バナジー，グータミ
Original Assignee
バイオメティス テクノロジー，インコーポレイテッド
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Priority to JP2025159783A priority Critical patent/JP2026004384A/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02—Polyesters derived from dicarboxylic acids and dihydroxy compounds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/62—Plastics recycling; Rubber recycling

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sustainable Development (AREA)
  • Polymers & Plastics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Separation, Recovery Or Treatment Of Waste Materials Containing Plastics (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Confectionery (AREA)
JP2024508603A 2021-08-11 2022-08-11 ポリエチレンテレフタレートの酵素分解 Active JP7797623B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025159783A JP2026004384A (ja) 2021-08-11 2025-09-26 ポリエチレンテレフタレートの酵素分解

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163232122P 2021-08-11 2021-08-11
US63/232,122 2021-08-11
PCT/US2022/074866 WO2023019222A1 (en) 2021-08-11 2022-08-11 Enzymatic degradation of polyethylene terephthalate

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025159783A Division JP2026004384A (ja) 2021-08-11 2025-09-26 ポリエチレンテレフタレートの酵素分解

Publications (2)

Publication Number Publication Date
JP2024531226A JP2024531226A (ja) 2024-08-29
JP7797623B2 true JP7797623B2 (ja) 2026-01-13

Family

ID=85201000

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2024508603A Active JP7797623B2 (ja) 2021-08-11 2022-08-11 ポリエチレンテレフタレートの酵素分解
JP2025159783A Pending JP2026004384A (ja) 2021-08-11 2025-09-26 ポリエチレンテレフタレートの酵素分解

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2025159783A Pending JP2026004384A (ja) 2021-08-11 2025-09-26 ポリエチレンテレフタレートの酵素分解

Country Status (9)

Country Link
US (1) US20250368971A1 (https=)
EP (1) EP4384607A4 (https=)
JP (2) JP7797623B2 (https=)
KR (1) KR20240032968A (https=)
CN (1) CN117836407A (https=)
AU (1) AU2022325230B2 (https=)
CA (1) CA3228057A1 (https=)
MX (1) MX2024001814A (https=)
WO (1) WO2023019222A1 (https=)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20240270924A1 (en) * 2023-02-09 2024-08-15 Laura KOLINSKY Residential Plastic Recycling Unit
CN121175416A (zh) * 2023-05-15 2025-12-19 卡比奥斯公司 酯酶变体及其在聚酯降解中的用途
TW202511480A (zh) * 2023-05-15 2025-03-16 法商卡爾畢歐斯公司 新穎之酯酶及其用途
KR20240167733A (ko) * 2023-05-18 2024-11-28 주식회사 자이엔 Pet 분해 활성을 갖는 변이형 폴리펩티드
IT202300014223A1 (it) * 2023-07-07 2025-01-07 Milano Politecnico Enzima ingegnerizzato termostabile
WO2025015288A1 (en) * 2023-07-12 2025-01-16 Triad National Security, Llc Methods to improve enzymes that degrade polyethylene terephthalate
CN119842650A (zh) * 2023-10-16 2025-04-18 中国科学院天津工业生物技术研究所 一种合成聚酯中间体的酰基转移酶及其应用
WO2025128953A1 (en) * 2023-12-15 2025-06-19 Flagship Pioneering Innovations Vii, Llc Esterases and related methods
WO2025129034A2 (en) * 2023-12-15 2025-06-19 Flagship Pioneering Innovations Vii, Llc Esterases and related methods
WO2025128934A1 (en) * 2023-12-15 2025-06-19 Flagship Pioneering Innovations Vii, Llc Esterases and related methods
WO2025136384A1 (en) * 2023-12-20 2025-06-26 Biometis Technology, Inc. Enzymatic degradation of polyethylene terephthalate
KR20250115684A (ko) * 2024-01-24 2025-07-31 씨제이제일제당 (주) 폴리에스테르 효소 분해 공정에서 TPA(terephthalic acid)를 효율적으로 회수하는 공정
WO2025254706A1 (en) * 2024-06-07 2025-12-11 Biometis Technology, Inc. Methods of plastic pretreatment and depolymerization
WO2026034323A1 (ja) * 2024-08-06 2026-02-12 東洋紡株式会社 エチレンテレフタレート化合物分解酵素
CN120905186B (zh) * 2025-09-28 2026-03-17 源天生物科技(天津)有限公司 BhrPETase突变体及其应用
CN121406605A (zh) * 2025-12-30 2026-01-27 源天生物科技(天津)有限公司 一种KL-CCF-MHETase突变体及其相关生物材料和应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015119670A (ja) 2013-12-24 2015-07-02 国立大学法人京都工芸繊維大学 新規なクチナーゼ、クチナーゼをコードする遺伝子、及びクチナーゼを用いたポリエステル又はエステル化合物の分解方法
WO2017198786A1 (en) 2016-05-19 2017-11-23 Carbios A process for degrading plastic products
JP2018500003A (ja) 2014-10-21 2018-01-11 キャルビオスＣａｒｂｉｏｓ ポリエステル分解活性を有するポリペプチド及びその使用
WO2020021118A1 (en) 2018-07-27 2020-01-30 Carbios Novel esterases and uses thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102017214870A1 (de) * 2017-08-24 2019-03-14 Henkel Ag & Co. Kgaa Verbesserte Pflegeeigenschaften von Polyester Textilien II
WO2019168811A1 (en) * 2018-02-28 2019-09-06 Alliance For Sustainable Energy, Llc Enzymes for polymer degradation
WO2021081633A1 (en) * 2019-10-28 2021-05-06 The Royal Institution For The Advancement Of Learning / Mcgill University Mechanoenzymatic degradation of polymers
WO2021145822A1 (en) * 2020-01-16 2021-07-22 Agency For Science, Technology And Research Thermostable petase enzyme
CN115125225B (zh) * 2021-03-25 2023-07-04 湖北大学 具有提升的热稳定性的pet降解酶

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015119670A (ja) 2013-12-24 2015-07-02 国立大学法人京都工芸繊維大学 新規なクチナーゼ、クチナーゼをコードする遺伝子、及びクチナーゼを用いたポリエステル又はエステル化合物の分解方法
JP2018500003A (ja) 2014-10-21 2018-01-11 キャルビオスＣａｒｂｉｏｓ ポリエステル分解活性を有するポリペプチド及びその使用
WO2017198786A1 (en) 2016-05-19 2017-11-23 Carbios A process for degrading plastic products
WO2020021118A1 (en) 2018-07-27 2020-01-30 Carbios Novel esterases and uses thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Kato, S., et al.，ACCESSION No. GBD22443, DEFINITION: Poly(ethylene terephthalate) hydrolase [bacterium HR29].，Database GenBank [online]，2018年，インターネット <URL: https://www.ncbi.nlm.nih.gov/protein/GBD22443.1?report=genbank&log$=protalign&blast_rank=1&RID=XHGWUJZY013>，08-JUN-2018 uploaded, [検索日 2025.03.18]
Nature，2020年，Vol.580, No.7802，pp.216-219
Schmidt, J., et al.，ACCESSION No. CUM11140, DEFINITION: LC cutinase [synthetic construct].，Database GenBank [online]，2015年，インターネット <URL: https://www.ncbi.nlm.nih.gov/protein/CUM11140.1?report=genbank&log$=protalign&blast_rank=3&RID=XHGWUJZY013>，01-OCT-2015 uploaded, [検索日 2025.03.18]
化学と生物，2020年，Vol.58, No.6，pp.362-368

Also Published As

Publication number Publication date
US20250368971A1 (en) 2025-12-04
AU2022325230B2 (en) 2025-09-11
MX2024001814A (es) 2024-03-01
KR20240032968A (ko) 2024-03-12
WO2023019222A1 (en) 2023-02-16
CN117836407A (zh) 2024-04-05
JP2024531226A (ja) 2024-08-29
CA3228057A1 (en) 2023-02-16
EP4384607A4 (en) 2025-09-03
EP4384607A1 (en) 2024-06-19
JP2026004384A (ja) 2026-01-14
AU2022325230A1 (en) 2024-02-15

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