CN117836407A - 聚对苯二甲酸乙二醇酯的酶促降解 - Google Patents

聚对苯二甲酸乙二醇酯的酶促降解 Download PDF

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Publication number
CN117836407A
CN117836407A CN202280055812.9A CN202280055812A CN117836407A CN 117836407 A CN117836407 A CN 117836407A CN 202280055812 A CN202280055812 A CN 202280055812A CN 117836407 A CN117836407 A CN 117836407A
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CN
China
Prior art keywords
amino acid
acid substitution
petase
bhr
variant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202280055812.9A
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English (en)
Chinese (zh)
Inventor
J·杨
X·张
K·奥
G·班纳吉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomedia Technology Co
Original Assignee
Biomedia Technology Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomedia Technology Co filed Critical Biomedia Technology Co
Publication of CN117836407A publication Critical patent/CN117836407A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02—Polyesters derived from dicarboxylic acids and dihydroxy compounds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/62—Plastics recycling; Rubber recycling

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sustainable Development (AREA)
  • Polymers & Plastics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Separation, Recovery Or Treatment Of Waste Materials Containing Plastics (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Confectionery (AREA)
CN202280055812.9A 2021-08-11 2022-08-11 聚对苯二甲酸乙二醇酯的酶促降解 Pending CN117836407A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163232122P 2021-08-11 2021-08-11
US63/232,122 2021-08-11
PCT/US2022/074866 WO2023019222A1 (en) 2021-08-11 2022-08-11 Enzymatic degradation of polyethylene terephthalate

Publications (1)

Publication Number Publication Date
CN117836407A true CN117836407A (zh) 2024-04-05

Family

ID=85201000

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202280055812.9A Pending CN117836407A (zh) 2021-08-11 2022-08-11 聚对苯二甲酸乙二醇酯的酶促降解

Country Status (9)

Country Link
US (1) US20250368971A1 (https=)
EP (1) EP4384607A4 (https=)
JP (2) JP7797623B2 (https=)
KR (1) KR20240032968A (https=)
CN (1) CN117836407A (https=)
AU (1) AU2022325230B2 (https=)
CA (1) CA3228057A1 (https=)
MX (1) MX2024001814A (https=)
WO (1) WO2023019222A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120905186A (zh) * 2025-09-28 2025-11-07 源天生物科技(天津)有限公司 BhrPETase突变体及其应用

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20240270924A1 (en) * 2023-02-09 2024-08-15 Laura KOLINSKY Residential Plastic Recycling Unit
CN121175416A (zh) * 2023-05-15 2025-12-19 卡比奥斯公司 酯酶变体及其在聚酯降解中的用途
TW202511480A (zh) * 2023-05-15 2025-03-16 法商卡爾畢歐斯公司 新穎之酯酶及其用途
KR20240167733A (ko) * 2023-05-18 2024-11-28 주식회사 자이엔 Pet 분해 활성을 갖는 변이형 폴리펩티드
IT202300014223A1 (it) * 2023-07-07 2025-01-07 Milano Politecnico Enzima ingegnerizzato termostabile
WO2025015288A1 (en) * 2023-07-12 2025-01-16 Triad National Security, Llc Methods to improve enzymes that degrade polyethylene terephthalate
CN119842650A (zh) * 2023-10-16 2025-04-18 中国科学院天津工业生物技术研究所 一种合成聚酯中间体的酰基转移酶及其应用
WO2025128953A1 (en) * 2023-12-15 2025-06-19 Flagship Pioneering Innovations Vii, Llc Esterases and related methods
WO2025129034A2 (en) * 2023-12-15 2025-06-19 Flagship Pioneering Innovations Vii, Llc Esterases and related methods
WO2025128934A1 (en) * 2023-12-15 2025-06-19 Flagship Pioneering Innovations Vii, Llc Esterases and related methods
WO2025136384A1 (en) * 2023-12-20 2025-06-26 Biometis Technology, Inc. Enzymatic degradation of polyethylene terephthalate
KR20250115684A (ko) * 2024-01-24 2025-07-31 씨제이제일제당 (주) 폴리에스테르 효소 분해 공정에서 TPA(terephthalic acid)를 효율적으로 회수하는 공정
WO2025254706A1 (en) * 2024-06-07 2025-12-11 Biometis Technology, Inc. Methods of plastic pretreatment and depolymerization
WO2026034323A1 (ja) * 2024-08-06 2026-02-12 東洋紡株式会社 エチレンテレフタレート化合物分解酵素
CN121406605A (zh) * 2025-12-30 2026-01-27 源天生物科技(天津)有限公司 一种KL-CCF-MHETase突变体及其相关生物材料和应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6315978B2 (ja) 2013-12-24 2018-04-25 合同会社ミクロバイオ開発研究所 新規なクチナーゼ、クチナーゼをコードする遺伝子、及びクチナーゼを用いたポリエステル又はエステル化合物の分解方法
TN2017000085A1 (en) 2014-10-21 2018-07-04 Carbios Polypeptide having a polyester degrading activity and uses thereof
MX2018014090A (es) 2016-05-19 2019-04-01 Carbios Un proceso para degradar productos de plastico.
DE102017214870A1 (de) * 2017-08-24 2019-03-14 Henkel Ag & Co. Kgaa Verbesserte Pflegeeigenschaften von Polyester Textilien II
WO2019168811A1 (en) * 2018-02-28 2019-09-06 Alliance For Sustainable Energy, Llc Enzymes for polymer degradation
JP7601756B2 (ja) * 2018-07-27 2024-12-17 キャルビオス 新規なエステラーゼ及びその使用
WO2021081633A1 (en) * 2019-10-28 2021-05-06 The Royal Institution For The Advancement Of Learning / Mcgill University Mechanoenzymatic degradation of polymers
WO2021145822A1 (en) * 2020-01-16 2021-07-22 Agency For Science, Technology And Research Thermostable petase enzyme
CN115125225B (zh) * 2021-03-25 2023-07-04 湖北大学 具有提升的热稳定性的pet降解酶

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120905186A (zh) * 2025-09-28 2025-11-07 源天生物科技(天津)有限公司 BhrPETase突变体及其应用
CN120905186B (zh) * 2025-09-28 2026-03-17 源天生物科技(天津)有限公司 BhrPETase突变体及其应用

Also Published As

Publication number Publication date
US20250368971A1 (en) 2025-12-04
JP7797623B2 (ja) 2026-01-13
AU2022325230B2 (en) 2025-09-11
MX2024001814A (es) 2024-03-01
KR20240032968A (ko) 2024-03-12
WO2023019222A1 (en) 2023-02-16
JP2024531226A (ja) 2024-08-29
CA3228057A1 (en) 2023-02-16
EP4384607A4 (en) 2025-09-03
EP4384607A1 (en) 2024-06-19
JP2026004384A (ja) 2026-01-14
AU2022325230A1 (en) 2024-02-15

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