JP7716773B2 - ｉＰＳ細胞由来軟骨細胞構造体の製造方法 - Google Patents

ｉＰＳ細胞由来軟骨細胞構造体の製造方法

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Publication number
JP7716773B2
JP7716773B2 JP2023532097A JP2023532097A JP7716773B2 JP 7716773 B2 JP7716773 B2 JP 7716773B2 JP 2023532097 A JP2023532097 A JP 2023532097A JP 2023532097 A JP2023532097 A JP 2023532097A JP 7716773 B2 JP7716773 B2 JP 7716773B2
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cells
cell
day
stem cells
differentiation
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JPWO2023277195A1 (https=
JPWO2023277195A5 (https=
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功一 中山
アンナマリア 中村
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Saga University NUC
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Saga University NUC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y10/00—Processes of additive manufacturing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Materials Engineering (AREA)
  • Manufacturing & Machinery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
JP2023532097A 2021-06-29 2022-06-28 ｉＰＳ細胞由来軟骨細胞構造体の製造方法 Active JP7716773B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021107656 2021-06-29
JP2021107656 2021-06-29
PCT/JP2022/026536 WO2023277195A1 (ja) 2021-06-29 2022-06-28 ｉＰＳ細胞由来軟骨細胞構造体の製造方法

Publications (3)

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JPWO2023277195A1 JPWO2023277195A1 (https=) 2023-01-05
JPWO2023277195A5 JPWO2023277195A5 (https=) 2024-03-25
JP7716773B2 true JP7716773B2 (ja) 2025-08-01

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JP2023532097A Active JP7716773B2 (ja) 2021-06-29 2022-06-28 ｉＰＳ細胞由来軟骨細胞構造体の製造方法

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Country Link
US (1) US20240294877A1 (https=)
EP (1) EP4365281A4 (https=)
JP (1) JP7716773B2 (https=)
CN (1) CN117561328A (https=)
WO (1) WO2023277195A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE1031503B1 (fr) * 2023-04-14 2024-11-13 Novadip Biosciences Composition comprenant une matrice extracellulaire neo-synthesisée déivée de cellules stem pluripotentes induites (ipsc)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008123614A1 (ja) 2007-03-30 2008-10-16 Kyushu University, National University Corporation 細胞の立体構造体の製造方法
JP2020202785A (ja) 2019-06-17 2020-12-24 株式会社サイフューズ 細胞立体構造体及びその製造方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8048999B2 (en) 2005-12-13 2011-11-01 Kyoto University Nuclear reprogramming factor

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008123614A1 (ja) 2007-03-30 2008-10-16 Kyushu University, National University Corporation 細胞の立体構造体の製造方法
JP2020202785A (ja) 2019-06-17 2020-12-24 株式会社サイフューズ 細胞立体構造体及びその製造方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Britta J.H Drissen et al，Cellular reprogramming for clinical cartilage repair，Cell Biol Toxicol，2017年01月31日，Vol.33，pp.329-349，DOI 10.1007/s10565-017-9382-0
MURATA Daiki et al，Osteochondral Regeneration Using Adipose Tissue-Derived Mesenchymal Stem Cells，Int. J. Mol. Sci，2020年05月19日，Vol.21，pp.1-23，doi:10.3390/ijms21103589
UMEDA Katsutsugu et al，Human chondrogenic paraxial mesoderm, directed specification and prospective isolation from pluripotent stem cells，SCIENTIFIC REPORTS，2012年06月13日，Vol.2，pp.1-11，DOI: 10.1038/srep00455

Also Published As

Publication number Publication date
EP4365281A4 (en) 2025-07-16
EP4365281A1 (en) 2024-05-08
JPWO2023277195A1 (https=) 2023-01-05
WO2023277195A1 (ja) 2023-01-05
US20240294877A1 (en) 2024-09-05
CN117561328A (zh) 2024-02-13

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