JP7713448B2 - 多分子ルシフェラーゼペプチド及びポリペプチド - Google Patents

多分子ルシフェラーゼペプチド及びポリペプチド

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Publication number
JP7713448B2
JP7713448B2 JP2022531486A JP2022531486A JP7713448B2 JP 7713448 B2 JP7713448 B2 JP 7713448B2 JP 2022531486 A JP2022531486 A JP 2022531486A JP 2022531486 A JP2022531486 A JP 2022531486A JP 7713448 B2 JP7713448 B2 JP 7713448B2
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JP
Japan
Prior art keywords
seq
peptide
protein
polypeptide
sequence identity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2022531486A
Other languages
English (en)
Japanese (ja)
Other versions
JP2023503653A5 (https=
JP2023503653A (ja
Inventor
バージニア キンケイド
ランス ピー エンセル
メアリー ホール
マイケル キロラン
キース ウッド
メラニー ダート
トリシュ ホアン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of JP2023503653A publication Critical patent/JP2023503653A/ja
Publication of JP2023503653A5 publication Critical patent/JP2023503653A5/ja
Priority to JP2025118267A priority Critical patent/JP2025162556A/ja
Application granted granted Critical
Publication of JP7713448B2 publication Critical patent/JP7713448B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G01N21/763—Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12005—Renilla-luciferin 2-monooxygenase (1.13.12.5), i.e. renilla-luciferase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Plasma & Fusion (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Cosmetics (AREA)
JP2022531486A 2019-11-27 2020-11-27 多分子ルシフェラーゼペプチド及びポリペプチド Active JP7713448B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025118267A JP2025162556A (ja) 2019-11-27 2025-07-14 多分子ルシフェラーゼペプチド及びポリペプチド

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962941255P 2019-11-27 2019-11-27
US62/941,255 2019-11-27
PCT/US2020/062499 WO2021108765A1 (en) 2019-11-27 2020-11-27 Multipartite luciferase peptides and polypeptides

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025118267A Division JP2025162556A (ja) 2019-11-27 2025-07-14 多分子ルシフェラーゼペプチド及びポリペプチド

Publications (3)

Publication Number Publication Date
JP2023503653A JP2023503653A (ja) 2023-01-31
JP2023503653A5 JP2023503653A5 (https=) 2023-12-05
JP7713448B2 true JP7713448B2 (ja) 2025-07-25

Family

ID=74106118

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2022531486A Active JP7713448B2 (ja) 2019-11-27 2020-11-27 多分子ルシフェラーゼペプチド及びポリペプチド
JP2025118267A Pending JP2025162556A (ja) 2019-11-27 2025-07-14 多分子ルシフェラーゼペプチド及びポリペプチド

Family Applications After (1)

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JP2025118267A Pending JP2025162556A (ja) 2019-11-27 2025-07-14 多分子ルシフェラーゼペプチド及びポリペプチド

Country Status (10)

Country Link
US (2) US12241839B2 (https=)
EP (1) EP4065699A1 (https=)
JP (2) JP7713448B2 (https=)
KR (1) KR20220121816A (https=)
CN (1) CN115427556A (https=)
AU (1) AU2020392245A1 (https=)
BR (1) BR112022010153A2 (https=)
CA (1) CA3158729A1 (https=)
IL (1) IL293204A (https=)
WO (1) WO2021108765A1 (https=)

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Publication number Priority date Publication date Assignee Title
EP3395803A1 (en) 2017-04-28 2018-10-31 Institut Pasteur Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins
EP3941195A4 (en) 2019-03-20 2023-03-22 Promega Corporation PHOTOAFFINITY PROBES
US20240003881A1 (en) * 2020-12-04 2024-01-04 The Governing Council Of The University Of Toronto System and method for rapid and sensitive detection of anti-pathogen antibodies
EP4337646A4 (en) 2021-05-13 2025-05-21 Promega Corporation BIOLUMINESCENT DETECTION OF DNA SYNTHESIS
WO2023039535A1 (en) * 2021-09-09 2023-03-16 Trustees Of Tufts College Outer membrane vesicle (omv)-based vaccines against sudden acute respiratory syndrome coronavirus 2 (sars-cov-2) spike protein
KR20240154535A (ko) * 2022-01-17 2024-10-25 유니버시티 오브 워싱턴 새롭게 설계된 루시퍼라제
WO2023215497A1 (en) 2022-05-04 2023-11-09 Promega Corporation Photoactivatable compounds and uses thereof
GB2619059A (en) * 2022-05-26 2023-11-29 Univ Leeds Innovations Ltd Split reporter complex
AU2024265176A1 (en) * 2023-05-04 2025-12-11 Promega Corporation Complementation-based tags and reporters for dual-modality labeling
WO2025064581A1 (en) * 2023-09-18 2025-03-27 The Brigham And Women’S Hospital, Inc. Biosensor platform for the rapid detection of analytes

Citations (1)

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US20190383808A1 (en) 2018-06-14 2019-12-19 The Trustees Of Columbia University In The City New York Assays using arrestin recruitment and unmodified receptors

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BR112013010487B1 (pt) 2010-11-02 2021-02-02 Promega Corporation compostos de coelenterazina, kit compreendendo ditos compostos e método para detectar luminescência em uma amostra in vitro
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Also Published As

Publication number Publication date
CA3158729A1 (en) 2021-06-03
CN115427556A (zh) 2022-12-02
KR20220121816A (ko) 2022-09-01
IL293204A (en) 2022-07-01
US12241839B2 (en) 2025-03-04
BR112022010153A2 (pt) 2022-08-09
EP4065699A1 (en) 2022-10-05
US20250297961A1 (en) 2025-09-25
WO2021108765A1 (en) 2021-06-03
JP2023503653A (ja) 2023-01-31
US20210262941A1 (en) 2021-08-26
JP2025162556A (ja) 2025-10-27
AU2020392245A1 (en) 2022-06-23

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