AU2020392245A1 - Multipartite luciferase peptides and polypeptides - Google Patents

Multipartite luciferase peptides and polypeptides Download PDF

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Publication number
AU2020392245A1
AU2020392245A1 AU2020392245A AU2020392245A AU2020392245A1 AU 2020392245 A1 AU2020392245 A1 AU 2020392245A1 AU 2020392245 A AU2020392245 A AU 2020392245A AU 2020392245 A AU2020392245 A AU 2020392245A AU 2020392245 A1 AU2020392245 A1 AU 2020392245A1
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AU
Australia
Prior art keywords
seq
protein
peptide
polypeptide
sequence identity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
AU2020392245A
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English (en)
Inventor
Melanie Dart
Lance P. Encell
Mary Hall
Trish HOANG
Michael Killoran
Virginia Kincaid
Keith Wood
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of AU2020392245A1 publication Critical patent/AU2020392245A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G01N21/763—Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12005—Renilla-luciferin 2-monooxygenase (1.13.12.5), i.e. renilla-luciferase

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Plasma & Fusion (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Cosmetics (AREA)
AU2020392245A 2019-11-27 2020-11-27 Multipartite luciferase peptides and polypeptides Pending AU2020392245A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962941255P 2019-11-27 2019-11-27
US62/941,255 2019-11-27
PCT/US2020/062499 WO2021108765A1 (en) 2019-11-27 2020-11-27 Multipartite luciferase peptides and polypeptides

Publications (1)

Publication Number Publication Date
AU2020392245A1 true AU2020392245A1 (en) 2022-06-23

Family

ID=74106118

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2020392245A Pending AU2020392245A1 (en) 2019-11-27 2020-11-27 Multipartite luciferase peptides and polypeptides

Country Status (10)

Country Link
US (2) US12241839B2 (https=)
EP (1) EP4065699A1 (https=)
JP (2) JP7713448B2 (https=)
KR (1) KR20220121816A (https=)
CN (1) CN115427556A (https=)
AU (1) AU2020392245A1 (https=)
BR (1) BR112022010153A2 (https=)
CA (1) CA3158729A1 (https=)
IL (1) IL293204A (https=)
WO (1) WO2021108765A1 (https=)

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EP3395803A1 (en) 2017-04-28 2018-10-31 Institut Pasteur Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins
EP3941195A4 (en) 2019-03-20 2023-03-22 Promega Corporation PHOTOAFFINITY PROBES
US20240003881A1 (en) * 2020-12-04 2024-01-04 The Governing Council Of The University Of Toronto System and method for rapid and sensitive detection of anti-pathogen antibodies
EP4337646A4 (en) 2021-05-13 2025-05-21 Promega Corporation BIOLUMINESCENT DETECTION OF DNA SYNTHESIS
WO2023039535A1 (en) * 2021-09-09 2023-03-16 Trustees Of Tufts College Outer membrane vesicle (omv)-based vaccines against sudden acute respiratory syndrome coronavirus 2 (sars-cov-2) spike protein
KR20240154535A (ko) * 2022-01-17 2024-10-25 유니버시티 오브 워싱턴 새롭게 설계된 루시퍼라제
WO2023215497A1 (en) 2022-05-04 2023-11-09 Promega Corporation Photoactivatable compounds and uses thereof
GB2619059A (en) * 2022-05-26 2023-11-29 Univ Leeds Innovations Ltd Split reporter complex
AU2024265176A1 (en) * 2023-05-04 2025-12-11 Promega Corporation Complementation-based tags and reporters for dual-modality labeling
WO2025064581A1 (en) * 2023-09-18 2025-03-27 The Brigham And Women’S Hospital, Inc. Biosensor platform for the rapid detection of analytes

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EP0281604B1 (en) 1986-09-02 1993-03-31 Enzon Labs Inc. Single polypeptide chain binding molecules
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
US5260203A (en) 1986-09-02 1993-11-09 Enzon, Inc. Single polypeptide chain binding molecules
US5914095A (en) 1989-04-07 1999-06-22 Salutar, Inc. Polychelants containg amide bonds
US5368484A (en) 1992-05-22 1994-11-29 Atari Games Corp. Vehicle simulator with realistic operating feedback
US5756688A (en) 1992-10-14 1998-05-26 Sterling Winthrop Inc. MR imaging compositions and methods
WO1994009056A1 (en) 1992-10-14 1994-04-28 Sterling Winthrop Inc. Chelating polymers
GB9503969D0 (en) 1995-02-28 1995-04-19 Sams Bernard Incrementing mechanism
US7268229B2 (en) 2001-11-02 2007-09-11 Promega Corporation Compounds to co-localize luminophores with luminescent proteins
US20080248511A1 (en) 2007-03-26 2008-10-09 Promega Corporation Methods to quench light from optical reactions
LT3409764T (lt) 2009-05-01 2020-06-10 Promega Corporation Sintetinės oplophorus liuciferazės su sustiprinta šviesos išeiga
TW201105646A (en) 2009-05-28 2011-02-16 Solvay Fluor Gmbh Process for the preparation of 4-fluoro-4-R-5-R'-1,3-dioxolane-2-ones
BR112013010487B1 (pt) 2010-11-02 2021-02-02 Promega Corporation compostos de coelenterazina, kit compreendendo ditos compostos e método para detectar luminescência em uma amostra in vitro
WO2012061477A1 (en) 2010-11-02 2012-05-10 Promega Corporation Coelenterazine derivatives and methods of using same
EP2782916B1 (en) 2011-11-21 2018-02-28 Promega Corporation Carboxy x rhodamine analogs
WO2013177255A2 (en) 2012-05-24 2013-11-28 Connecticut College Chimeric luciferases
AU2013266968B2 (en) 2012-05-25 2017-06-29 Emmanuelle CHARPENTIER Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription
US20140099654A1 (en) 2012-09-26 2014-04-10 Promega Corporation Real-time monitoring
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SG11201507306VA (en) 2013-03-15 2015-10-29 Promega Corp Activation of bioluminescence by structural complementation
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US9908918B2 (en) 2015-06-30 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University Cyan-excitable orange-red fluorescent proteins and bioluminescent resonance energy transfer systems
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PL3759233T4 (pl) 2018-02-26 2022-10-10 Technische Universiteit Eindhoven Bioczujnik bioluminescencyjny do wykrywania i oznaczania ilościowego biocząsteczek
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US10962538B2 (en) * 2018-06-14 2021-03-30 The Trustees Of Columbia University In The City Of New York Assays using arrestin recruitment and unmodified receptors
BR112021020354A2 (pt) 2019-04-10 2021-12-14 Promega Corp Composições e métodos para detecção de analito com o uso de bioluminescência

Also Published As

Publication number Publication date
CA3158729A1 (en) 2021-06-03
CN115427556A (zh) 2022-12-02
KR20220121816A (ko) 2022-09-01
IL293204A (en) 2022-07-01
US12241839B2 (en) 2025-03-04
BR112022010153A2 (pt) 2022-08-09
JP7713448B2 (ja) 2025-07-25
EP4065699A1 (en) 2022-10-05
US20250297961A1 (en) 2025-09-25
WO2021108765A1 (en) 2021-06-03
JP2023503653A (ja) 2023-01-31
US20210262941A1 (en) 2021-08-26
JP2025162556A (ja) 2025-10-27

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