CN115427556A - 多部分萤光素酶肽和多肽 - Google Patents

多部分萤光素酶肽和多肽 Download PDF

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Publication number
CN115427556A
CN115427556A CN202080095195.6A CN202080095195A CN115427556A CN 115427556 A CN115427556 A CN 115427556A CN 202080095195 A CN202080095195 A CN 202080095195A CN 115427556 A CN115427556 A CN 115427556A
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China
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seq
protein
peptide
polypeptide
sequence identity
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Pending
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CN202080095195.6A
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English (en)
Chinese (zh)
Inventor
V·金凯德
L·P·恩塞尔
M·霍尔
M·基洛兰
K·伍德
M·达特
T·黄
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Promega Corp
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Promega Corp
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Publication of CN115427556A publication Critical patent/CN115427556A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G01N21/763—Bioluminescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12005—Renilla-luciferin 2-monooxygenase (1.13.12.5), i.e. renilla-luciferase

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Plasma & Fusion (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Cosmetics (AREA)
CN202080095195.6A 2019-11-27 2020-11-27 多部分萤光素酶肽和多肽 Pending CN115427556A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962941255P 2019-11-27 2019-11-27
US62/941,255 2019-11-27
PCT/US2020/062499 WO2021108765A1 (en) 2019-11-27 2020-11-27 Multipartite luciferase peptides and polypeptides

Publications (1)

Publication Number Publication Date
CN115427556A true CN115427556A (zh) 2022-12-02

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080095195.6A Pending CN115427556A (zh) 2019-11-27 2020-11-27 多部分萤光素酶肽和多肽

Country Status (10)

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US (2) US12241839B2 (https=)
EP (1) EP4065699A1 (https=)
JP (2) JP7713448B2 (https=)
KR (1) KR20220121816A (https=)
CN (1) CN115427556A (https=)
AU (1) AU2020392245A1 (https=)
BR (1) BR112022010153A2 (https=)
CA (1) CA3158729A1 (https=)
IL (1) IL293204A (https=)
WO (1) WO2021108765A1 (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3395803A1 (en) 2017-04-28 2018-10-31 Institut Pasteur Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins
EP3941195A4 (en) 2019-03-20 2023-03-22 Promega Corporation PHOTOAFFINITY PROBES
US20240003881A1 (en) * 2020-12-04 2024-01-04 The Governing Council Of The University Of Toronto System and method for rapid and sensitive detection of anti-pathogen antibodies
EP4337646A4 (en) 2021-05-13 2025-05-21 Promega Corporation BIOLUMINESCENT DETECTION OF DNA SYNTHESIS
WO2023039535A1 (en) * 2021-09-09 2023-03-16 Trustees Of Tufts College Outer membrane vesicle (omv)-based vaccines against sudden acute respiratory syndrome coronavirus 2 (sars-cov-2) spike protein
KR20240154535A (ko) * 2022-01-17 2024-10-25 유니버시티 오브 워싱턴 새롭게 설계된 루시퍼라제
WO2023215497A1 (en) 2022-05-04 2023-11-09 Promega Corporation Photoactivatable compounds and uses thereof
GB2619059A (en) * 2022-05-26 2023-11-29 Univ Leeds Innovations Ltd Split reporter complex
AU2024265176A1 (en) * 2023-05-04 2025-12-11 Promega Corporation Complementation-based tags and reporters for dual-modality labeling
WO2025064581A1 (en) * 2023-09-18 2025-03-27 The Brigham And Women’S Hospital, Inc. Biosensor platform for the rapid detection of analytes

Citations (5)

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US20150152395A1 (en) * 2012-05-24 2015-06-04 Connecticut College Chimeric luciferases
US20160282360A1 (en) * 2015-02-05 2016-09-29 Promega Corporation Luciferase-based thermal shift assays
US20180172692A1 (en) * 2016-04-26 2018-06-21 Andrew S. Dixon Target-binding activated split reporter systems for analyte detection and related components and methods
WO2019164402A1 (en) * 2018-02-26 2019-08-29 Technische Universiteit Eindhoven Bioluminescent biosensor for detecting and quantifying biomolecules
US20190352623A1 (en) * 2014-09-12 2019-11-21 Promega Corporation Internal protein tags

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EP0281604B1 (en) 1986-09-02 1993-03-31 Enzon Labs Inc. Single polypeptide chain binding molecules
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
US5260203A (en) 1986-09-02 1993-11-09 Enzon, Inc. Single polypeptide chain binding molecules
US5914095A (en) 1989-04-07 1999-06-22 Salutar, Inc. Polychelants containg amide bonds
US5368484A (en) 1992-05-22 1994-11-29 Atari Games Corp. Vehicle simulator with realistic operating feedback
US5756688A (en) 1992-10-14 1998-05-26 Sterling Winthrop Inc. MR imaging compositions and methods
WO1994009056A1 (en) 1992-10-14 1994-04-28 Sterling Winthrop Inc. Chelating polymers
GB9503969D0 (en) 1995-02-28 1995-04-19 Sams Bernard Incrementing mechanism
US7268229B2 (en) 2001-11-02 2007-09-11 Promega Corporation Compounds to co-localize luminophores with luminescent proteins
US20080248511A1 (en) 2007-03-26 2008-10-09 Promega Corporation Methods to quench light from optical reactions
LT3409764T (lt) 2009-05-01 2020-06-10 Promega Corporation Sintetinės oplophorus liuciferazės su sustiprinta šviesos išeiga
TW201105646A (en) 2009-05-28 2011-02-16 Solvay Fluor Gmbh Process for the preparation of 4-fluoro-4-R-5-R'-1,3-dioxolane-2-ones
BR112013010487B1 (pt) 2010-11-02 2021-02-02 Promega Corporation compostos de coelenterazina, kit compreendendo ditos compostos e método para detectar luminescência em uma amostra in vitro
WO2012061477A1 (en) 2010-11-02 2012-05-10 Promega Corporation Coelenterazine derivatives and methods of using same
EP2782916B1 (en) 2011-11-21 2018-02-28 Promega Corporation Carboxy x rhodamine analogs
AU2013266968B2 (en) 2012-05-25 2017-06-29 Emmanuelle CHARPENTIER Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription
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US20150152395A1 (en) * 2012-05-24 2015-06-04 Connecticut College Chimeric luciferases
US20190352623A1 (en) * 2014-09-12 2019-11-21 Promega Corporation Internal protein tags
US20160282360A1 (en) * 2015-02-05 2016-09-29 Promega Corporation Luciferase-based thermal shift assays
US20180172692A1 (en) * 2016-04-26 2018-06-21 Andrew S. Dixon Target-binding activated split reporter systems for analyte detection and related components and methods
WO2019164402A1 (en) * 2018-02-26 2019-08-29 Technische Universiteit Eindhoven Bioluminescent biosensor for detecting and quantifying biomolecules

Also Published As

Publication number Publication date
CA3158729A1 (en) 2021-06-03
KR20220121816A (ko) 2022-09-01
IL293204A (en) 2022-07-01
US12241839B2 (en) 2025-03-04
BR112022010153A2 (pt) 2022-08-09
JP7713448B2 (ja) 2025-07-25
EP4065699A1 (en) 2022-10-05
US20250297961A1 (en) 2025-09-25
WO2021108765A1 (en) 2021-06-03
JP2023503653A (ja) 2023-01-31
US20210262941A1 (en) 2021-08-26
JP2025162556A (ja) 2025-10-27
AU2020392245A1 (en) 2022-06-23

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