JP7651470B2 - 核酸を解析するための方法および組成物 - Google Patents

核酸を解析するための方法および組成物 Download PDF

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JP7651470B2
JP7651470B2 JP2021559245A JP2021559245A JP7651470B2 JP 7651470 B2 JP7651470 B2 JP 7651470B2 JP 2021559245 A JP2021559245 A JP 2021559245A JP 2021559245 A JP2021559245 A JP 2021559245A JP 7651470 B2 JP7651470 B2 JP 7651470B2
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scaffold
nucleic acid
ssna
oligonucleotide
hybridization
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JP2022528139A5 (enExample
JP2022528139A (ja
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キンケイド， ケリー エム． ハーキンズ
コリン パトリック ノートン，
ヴァーシャ ラオ，
クリストファー ジェイ． トロル，
ジョシュア ディー． カップ，
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クラレット バイオサイエンス， エルエルシー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2021559245A 2019-04-05 2020-04-02 核酸を解析するための方法および組成物 Active JP7651470B2 (ja)

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JP2024189679A JP2025014018A (ja) 2019-04-05 2024-10-29 核酸を解析するための方法および組成物

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US201962830211P 2019-04-05 2019-04-05
US62/830,211 2019-04-05
US201962861594P 2019-06-14 2019-06-14
US62/861,594 2019-06-14
US201962925132P 2019-10-23 2019-10-23
US62/925,132 2019-10-23
PCT/US2020/026421 WO2020206143A1 (en) 2019-04-05 2020-04-02 Methods and compositions for analyzing nucleic acid

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EP (2) EP4538390A3 (enExample)
JP (2) JP7651470B2 (enExample)
KR (1) KR20210148304A (enExample)
CN (1) CN114174530A (enExample)
AU (1) AU2020253471B2 (enExample)
CA (1) CA3134831A1 (enExample)
IL (1) IL286945A (enExample)
SG (1) SG11202110468YA (enExample)
WO (1) WO2020206143A1 (enExample)

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WO2022020346A1 (en) 2020-07-21 2022-01-27 Claret Bioscience, Llc Cancer detection, monitoring, and reporting from sequencing cell-free dna
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US20260078439A1 (en) * 2022-08-24 2026-03-19 The Regents Of The University Of California Next-Generation Sequencing Pipeline for Detection of Ultrashort Single-Stranded Cell-Free DNA
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WO2024192634A1 (zh) * 2023-03-20 2024-09-26 深圳华大智造科技股份有限公司 一种高效且快速进行核酸文库构建的方法
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Publication number Publication date
EP4538390A2 (en) 2025-04-16
EP3947723C0 (en) 2024-12-18
KR20210148304A (ko) 2021-12-07
CA3134831A1 (en) 2020-10-08
EP3947723A1 (en) 2022-02-09
EP4538390A3 (en) 2025-07-09
IL286945A (en) 2021-12-01
CN114174530A (zh) 2022-03-11
JP2025014018A (ja) 2025-01-28
US20220348906A1 (en) 2022-11-03
WO2020206143A9 (en) 2021-12-23
EP3947723B1 (en) 2024-12-18
WO2020206143A1 (en) 2020-10-08
AU2020253471B2 (en) 2026-07-30
SG11202110468YA (en) 2021-10-28
JP2022528139A (ja) 2022-06-08
AU2020253471A1 (en) 2021-10-14

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