JP7644755B2 - Ｃａｓ１２ａヌクレアーゼの変異体ならびにその作製方法および使用方法 - Google Patents

Ｃａｓ１２ａヌクレアーゼの変異体ならびにその作製方法および使用方法 Download PDF

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JP7644755B2
JP7644755B2 JP2022523132A JP2022523132A JP7644755B2 JP 7644755 B2 JP7644755 B2 JP 7644755B2 JP 2022523132 A JP2022523132 A JP 2022523132A JP 2022523132 A JP2022523132 A JP 2022523132A JP 7644755 B2 JP7644755 B2 JP 7644755B2
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polypeptide
nucleic acid
lbcas12a
sequence
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ジョセフ マシュー ワッツ
サシャ シーラ ジャリ
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ペアーワイズ プランツ サービシズ， インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/20—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Spirochaetales (O), e.g. Treponema, Leptospira
    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04004—Adenosine deaminase (3.5.4.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005—Cytidine deaminase (3.5.4.5)

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  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2022523132A 2019-10-17 2020-10-15 Ｃａｓ１２ａヌクレアーゼの変異体ならびにその作製方法および使用方法 Active JP7644755B2 (ja)

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JP2025030119A JP2025087754A (ja) 2019-10-17 2025-02-27 Ｃａｓ１２ａヌクレアーゼの変異体ならびにその作製方法および使用方法

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US201962916392P 2019-10-17 2019-10-17
US62/916,392 2019-10-17
PCT/US2020/055659 WO2021076682A1 (en) 2019-10-17 2020-10-15 Variants of cas12a nucleases and methods of making and use thereof

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JP2022552409A JP2022552409A (ja) 2022-12-15
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JP2025030119A Pending JP2025087754A (ja) 2019-10-17 2025-02-27 Ｃａｓ１２ａヌクレアーゼの変異体ならびにその作製方法および使用方法

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US (3) US11866745B2 (https=)
EP (1) EP4045643A4 (https=)
JP (2) JP7644755B2 (https=)
KR (1) KR20220097408A (https=)
CN (1) CN114829595A (https=)
AU (1) AU2020366358A1 (https=)
BR (1) BR112022007125A2 (https=)
CA (1) CA3157707A1 (https=)
CL (4) CL2022000937A1 (https=)
IL (1) IL292117A (https=)
MX (1) MX2022004549A (https=)
WO (1) WO2021076682A1 (https=)

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CN110382692A (zh) * 2016-04-19 2019-10-25 博德研究所 新型crispr酶以及系统
WO2018022634A1 (en) 2016-07-26 2018-02-01 The General Hospital Corporation Variants of crispr from prevotella and francisella 1 (cpf1)
WO2022174108A1 (en) * 2021-02-12 2022-08-18 The Board Of Trustees Of The Leland Stanford Junior University Synthetic cas12a for enhanced multiplex gene control and editing
CN113136376B (zh) * 2021-05-26 2022-10-21 武汉大学 一种Cas12a变体及其在基因编辑中的应用
CA3230609A1 (en) * 2021-09-08 2023-03-16 Shaorong Chong Compositions comprising a crispr nuclease and uses thereof
CN114214348A (zh) * 2021-11-16 2022-03-22 河南农业大学 应用于核酸检测的Crispr/LbCas12a突变体蛋白及其制备方法和应用
CN118339285A (zh) * 2021-12-09 2024-07-12 北京干细胞与再生医学研究院 工程化Cas12b效应子蛋白及其使用方法
IL314074A (en) * 2022-01-06 2024-09-01 Ensoma Aps CAS12A endonuclease variants and methods of use
EP4508205A1 (en) * 2022-04-12 2025-02-19 John Innes Centre Compositions and methods for increasing genome editing efficiency
AR129505A1 (es) * 2022-06-01 2024-09-04 Inst Genetics & Developmental Biology Cas Novedoso sistema de edición de genes crispr
CN116286734B (zh) * 2022-11-29 2024-04-02 武汉大学 野生型LbCas12a蛋白的突变体及SNP检测用途
CN116144631B (zh) * 2023-01-17 2023-09-15 华中农业大学 耐热型核酸内切酶及其介导的基因编辑系统
WO2024182746A1 (en) 2023-03-01 2024-09-06 Pairwise Plants Services, Inc. Engineered proteins and methods of use thereof
CN116410955B (zh) * 2023-03-10 2023-12-19 华中农业大学 两种新型核酸内切酶及其在核酸检测中的应用
CN116179512B (zh) * 2023-03-16 2023-09-15 华中农业大学 靶标识别范围广的核酸内切酶及其应用
WO2025007140A2 (en) 2023-06-30 2025-01-02 Christiana Care Gene Editing Institute, Inc. Nras gene knockout for treatment of cancer
KR102789465B1 (ko) 2023-09-20 2025-04-04 주식회사 엔세이지 신규 CRISPR/Cas12a 시스템 및 그 유전자 편집 용도
CN119432813A (zh) * 2023-12-29 2025-02-14 武汉艾迪晶生物科技有限公司 Cas蛋白、组合蛋白、核酸分子、核糖核蛋白复合物、重组载体、转基因细胞及其应用
CN117844782B (zh) * 2024-03-06 2024-05-31 崖州湾国家实验室 靶向范围广的基因编辑核酸酶及其在核酸检测中的应用
US20250319206A1 (en) 2024-04-04 2025-10-16 Christina Care Gene Editing Institute, Inc. Crispr/cas gene editing of neh4 and/or neh5 domains in nrf2
WO2025226816A1 (en) 2024-04-23 2025-10-30 Christiana Care Gene Editing Institute, Inc. Methods of identifying and correlating crispr-induced exon skipping to phenotypic outcomes
WO2025244732A1 (en) * 2024-05-24 2025-11-27 The Regents Of The University Of California Variant cas12a protein compositions and methods
US20260009010A1 (en) 2024-07-08 2026-01-08 Pairwise Plants Services, Inc. Methods and compositions for modification of protospacer adjacent motif specificity of cas12a
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CN119506250B (zh) * 2024-11-29 2025-09-09 大连理工大学 一种多PAM选择的基因编辑系统NovaCas-2及其应用
CN119876148B (zh) * 2025-01-23 2025-10-17 中国科学技术大学 Crispr rna突变体及其在基因编辑和核酸检测中的应用
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WO2017184768A1 (en) 2016-04-19 2017-10-26 The Broad Institute Inc. Novel crispr enzymes and systems
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US20240076639A1 (en) 2024-03-07
WO2021076682A1 (en) 2021-04-22
CL2024001737A1 (es) 2024-11-15
US20240076640A1 (en) 2024-03-07
CN114829595A (zh) 2022-07-29
IL292117A (en) 2022-06-01
AU2020366358A1 (en) 2022-04-28
JP2025087754A (ja) 2025-06-10
CL2024001734A1 (es) 2024-11-15
US11866745B2 (en) 2024-01-09
KR20220097408A (ko) 2022-07-07
NZ787124A (en) 2025-09-26
BR112022007125A2 (pt) 2022-07-05
MX2022004549A (es) 2022-07-21
US20210115421A1 (en) 2021-04-22
EP4045643A1 (en) 2022-08-24
CA3157707A1 (en) 2021-04-22
JP2022552409A (ja) 2022-12-15
CL2022000937A1 (es) 2023-01-20
EP4045643A4 (en) 2023-11-08
CL2024001733A1 (es) 2024-11-15

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