CN114829595A - Cas12a核酸酶的变体及其制备方法和用途 - Google Patents

Cas12a核酸酶的变体及其制备方法和用途 Download PDF

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CN114829595A
CN114829595A CN202080087121.8A CN202080087121A CN114829595A CN 114829595 A CN114829595 A CN 114829595A CN 202080087121 A CN202080087121 A CN 202080087121A CN 114829595 A CN114829595 A CN 114829595A
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nucleic acid
sequence
lys
activity
polypeptide
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J·M·瓦特斯
S·S·贾里
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Pairing Plant Service Co ltd
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    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04004—Adenosine deaminase (3.5.4.4)
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    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005—Cytidine deaminase (3.5.4.5)

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  • Plant Pathology (AREA)
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  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN202080087121.8A 2019-10-17 2020-10-15 Cas12a核酸酶的变体及其制备方法和用途 Pending CN114829595A (zh)

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US201962916392P 2019-10-17 2019-10-17
US62/916,392 2019-10-17
PCT/US2020/055659 WO2021076682A1 (en) 2019-10-17 2020-10-15 Variants of cas12a nucleases and methods of making and use thereof

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US (3) US11866745B2 (https=)
EP (1) EP4045643A4 (https=)
JP (2) JP7644755B2 (https=)
KR (1) KR20220097408A (https=)
CN (1) CN114829595A (https=)
AU (1) AU2020366358A1 (https=)
BR (1) BR112022007125A2 (https=)
CA (1) CA3157707A1 (https=)
CL (4) CL2022000937A1 (https=)
IL (1) IL292117A (https=)
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WO2022174108A1 (en) * 2021-02-12 2022-08-18 The Board Of Trustees Of The Leland Stanford Junior University Synthetic cas12a for enhanced multiplex gene control and editing
CN113136376B (zh) * 2021-05-26 2022-10-21 武汉大学 一种Cas12a变体及其在基因编辑中的应用
CA3230609A1 (en) * 2021-09-08 2023-03-16 Shaorong Chong Compositions comprising a crispr nuclease and uses thereof
CN114214348A (zh) * 2021-11-16 2022-03-22 河南农业大学 应用于核酸检测的Crispr/LbCas12a突变体蛋白及其制备方法和应用
CN118339285A (zh) * 2021-12-09 2024-07-12 北京干细胞与再生医学研究院 工程化Cas12b效应子蛋白及其使用方法
IL314074A (en) * 2022-01-06 2024-09-01 Ensoma Aps CAS12A endonuclease variants and methods of use
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CN116286734B (zh) * 2022-11-29 2024-04-02 武汉大学 野生型LbCas12a蛋白的突变体及SNP检测用途
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WO2025007140A2 (en) 2023-06-30 2025-01-02 Christiana Care Gene Editing Institute, Inc. Nras gene knockout for treatment of cancer
KR102789465B1 (ko) 2023-09-20 2025-04-04 주식회사 엔세이지 신규 CRISPR/Cas12a 시스템 및 그 유전자 편집 용도
CN119432813A (zh) * 2023-12-29 2025-02-14 武汉艾迪晶生物科技有限公司 Cas蛋白、组合蛋白、核酸分子、核糖核蛋白复合物、重组载体、转基因细胞及其应用
CN117844782B (zh) * 2024-03-06 2024-05-31 崖州湾国家实验室 靶向范围广的基因编辑核酸酶及其在核酸检测中的应用
US20250319206A1 (en) 2024-04-04 2025-10-16 Christina Care Gene Editing Institute, Inc. Crispr/cas gene editing of neh4 and/or neh5 domains in nrf2
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WO2025244732A1 (en) * 2024-05-24 2025-11-27 The Regents Of The University Of California Variant cas12a protein compositions and methods
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WO2021076682A1 (en) 2021-04-22
CL2024001737A1 (es) 2024-11-15
US20240076640A1 (en) 2024-03-07
IL292117A (en) 2022-06-01
JP7644755B2 (ja) 2025-03-12
AU2020366358A1 (en) 2022-04-28
JP2025087754A (ja) 2025-06-10
CL2024001734A1 (es) 2024-11-15
US11866745B2 (en) 2024-01-09
KR20220097408A (ko) 2022-07-07
NZ787124A (en) 2025-09-26
BR112022007125A2 (pt) 2022-07-05
MX2022004549A (es) 2022-07-21
US20210115421A1 (en) 2021-04-22
EP4045643A1 (en) 2022-08-24
CA3157707A1 (en) 2021-04-22
JP2022552409A (ja) 2022-12-15
CL2022000937A1 (es) 2023-01-20
EP4045643A4 (en) 2023-11-08
CL2024001733A1 (es) 2024-11-15

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