JP7633149B2 - 新規なエステラーゼ及びその使用 - Google Patents

新規なエステラーゼ及びその使用 Download PDF

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Publication number
JP7633149B2
JP7633149B2 JP2021504396A JP2021504396A JP7633149B2 JP 7633149 B2 JP7633149 B2 JP 7633149B2 JP 2021504396 A JP2021504396 A JP 2021504396A JP 2021504396 A JP2021504396 A JP 2021504396A JP 7633149 B2 JP7633149 B2 JP 7633149B2
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Japan
Prior art keywords
esterase
polyester
seq
amino acid
present
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JP2021504396A
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English (en)
Japanese (ja)
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JP2021531032A5 (enExample
JP2021531032A (ja
Inventor
アンドレ,イザベル
トゥルニエ,ヴァンサン
ジル,アレクサンドル
ドゥケーヌ,ソフィー
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Carbios SA
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Carbios SA
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Publication of JP2021531032A5 publication Critical patent/JP2021531032A5/ja
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    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00Recovery or working-up of waste materials
    • C08J11/04Recovery or working-up of waste materials of polymers
    • C08J11/10Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02Polyesters derived from dicarboxylic acids and dihydroxy compounds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/01Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01074Cutinase (3.1.1.74)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/50Reuse, recycling or recovery technologies
    • Y02W30/62Plastics recycling; Rubber recycling

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Sustainable Development (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2021504396A 2018-07-27 2019-07-26 新規なエステラーゼ及びその使用 Active JP7633149B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP18306019 2018-07-27
EP18306019.3 2018-07-27
PCT/EP2019/070288 WO2020021117A1 (en) 2018-07-27 2019-07-26 Novel esterases and uses thereof

Publications (3)

Publication Number Publication Date
JP2021531032A JP2021531032A (ja) 2021-11-18
JP2021531032A5 JP2021531032A5 (enExample) 2022-08-03
JP7633149B2 true JP7633149B2 (ja) 2025-02-19

Family

ID=63144938

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2021504396A Active JP7633149B2 (ja) 2018-07-27 2019-07-26 新規なエステラーゼ及びその使用

Country Status (6)

Country Link
US (1) US11692181B2 (enExample)
EP (1) EP3830270A1 (enExample)
JP (1) JP7633149B2 (enExample)
KR (1) KR20210042122A (enExample)
CN (1) CN112673104B (enExample)
WO (1) WO2020021117A1 (enExample)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3307812B1 (en) 2015-06-12 2024-05-08 Carbios Biodegradable polyester composition and uses thereof
CA3024287C (en) 2016-05-19 2024-06-11 Carbios A process for degrading plastic products
EP3485007B1 (en) 2016-07-12 2025-10-29 Carbios Novel esterases and uses thereof
JP7179002B2 (ja) 2016-12-16 2022-11-28 キャルビオス 改良されたプラスチック分解プロテアーゼ
ES2901746T3 (es) 2017-08-31 2022-03-23 Carbios Composición líquida que comprende entidades biológicas y usos de la misma
KR20200103757A (ko) 2017-12-21 2020-09-02 까르비오 신규한 프로테아제 및 이의 용도
CA3107560A1 (en) 2018-07-27 2020-01-30 Carbios Esterases and uses thereof
PT3830254T (pt) 2018-07-27 2025-01-24 Carbios Novas esterases e suas utilizações
FR3088070B1 (fr) 2018-11-06 2021-11-26 Carbios Procede de degradation enzymatique de polyethylene terephtalate
AU2020249603A1 (en) 2019-03-28 2021-09-23 Carbios Multicomponent thermoplastic product
EP3997220B1 (en) 2019-07-11 2025-09-10 Carbios Esterases and uses thereof
EP3997219B1 (en) 2019-07-11 2025-08-06 Carbios Novel esterases and uses thereof
GB202005073D0 (en) 2020-04-06 2020-05-20 Mellizyme Biotechnology Ltd Enzymatic degradation of plastics
KR20230093468A (ko) 2020-10-27 2023-06-27 까르비오 신규한 에스테라아제 및 그 용도
DK181658B1 (en) * 2020-11-12 2024-09-11 Petroleo Brasileiro Sa Petrobras PROCESS FOR THE PRODUCTION OF GLYCOLIC ACID OR γ-HYDROXYBUTYRIC ACID INTEGRATED WITH THE BIOTECHNOLOGICAL DEPOLYMERISATION OF POLYETYLENE TEREPHTHALATE OR POLY(BUTYLENE SUCCINATE).
MX2023013755A (es) 2021-05-20 2023-12-04 Carbios Proceso para degradar un producto de plastico que comprende al menos un poliester.
CA3218310A1 (en) 2021-05-20 2022-11-24 Carbios Process for degrading a plastic product comprising at least one polyester
CN113698742B (zh) * 2021-08-31 2022-11-22 江南大学 一种聚对苯二甲酸乙二醇酯的改性方法及其在酶解过程中的应用
MX2024005891A (es) * 2021-11-16 2024-05-30 Carbios Esterasas y usos de las mismas.
EP4433582A2 (en) * 2021-11-16 2024-09-25 Carbios Esterases and uses thereof
US20250034347A1 (en) * 2021-11-16 2025-01-30 Carbios Use of esterases
WO2023242197A1 (en) 2022-06-13 2023-12-21 Carbios Process for purifying mono-ethylene glycol
CN115197927B (zh) * 2022-08-09 2024-06-21 武汉新华扬生物股份有限公司 一种降解生物基塑料的复合酶制剂及其应用
EP4321616A1 (en) 2022-08-09 2024-02-14 Covestro Deutschland AG Thermally stable carboxylic ester hydrolases in layered double hydroxides for intrinsic recyclable polymers
CN115287274B (zh) * 2022-10-08 2023-01-06 中国农业科学院生物技术研究所 角质酶iccg的突变体及其应用
WO2024151608A2 (en) 2023-01-09 2024-07-18 Protein Evolution Inc. Systems, methods, and compositions involving pretreatment and/or enzymatic degradation of crystallizable polymers, including copolymers
TW202506250A (zh) 2023-03-21 2025-02-16 美商蛋白質演化股份有限公司 從流體中分離顆粒的方法和組合物
WO2024236009A2 (en) 2023-05-15 2024-11-21 Carbios Novel esterases and uses thereof
KR20260011740A (ko) 2023-05-15 2026-01-23 까르비오 신규 에스테라제 및 이의 용도
CN117187212B (zh) * 2023-09-19 2025-02-11 源天生物科技(天津)有限公司 PET降解酶PET-mh突变体及其编码基因、重组质粒、工程菌和应用
CN117264927B (zh) * 2023-09-19 2024-11-29 源天生物科技(天津)有限公司 PET降解酶PET-mh29/124/183突变体及其编码基因、工程菌和应用
CN117448297B (zh) * 2023-09-19 2024-12-03 源天生物科技(天津)有限公司 PET降解酶PET-mh183/208突变体及其编码基因、工程菌和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018011281A1 (en) 2016-07-12 2018-01-18 Carbios Novel esterases and uses thereof
WO2018011284A1 (en) 2016-07-12 2018-01-18 Carbios Novel esterases and uses thereof

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5850342B2 (ja) * 2011-01-19 2016-02-03 天野エンザイム株式会社 枝葉コンポスト由来新規エステラーゼ
FR2984354A1 (fr) 2011-12-20 2013-06-21 Centre Nat Rech Scient Procede de preparation d'alliage polymere/enzymes
ES2707304T3 (es) 2012-11-20 2019-04-03 Carbios Método para reciclar productos de plástico
EP3065890A2 (en) 2013-11-05 2016-09-14 Carbios A method for degrading a plastic
DE102014204374A1 (de) 2014-03-11 2015-09-17 Henkel Ag & Co. Kgaa PET-Esterasen und deren Verwendung
CA2947478C (en) 2014-05-16 2022-09-13 Carbios Process of recycling mixed pet plastic articles
TN2017000085A1 (en) 2014-10-21 2018-07-04 Carbios Polypeptide having a polyester degrading activity and uses thereof
WO2016097325A1 (en) 2014-12-19 2016-06-23 Carbios Plastic compound and preparation process
CN107532153A (zh) 2015-03-13 2018-01-02 卡比奥斯公司 具有聚酯降解活性的新多肽及其用途
EP3307812B1 (en) 2015-06-12 2024-05-08 Carbios Biodegradable polyester composition and uses thereof
US10717996B2 (en) 2015-12-21 2020-07-21 Carbios Recombinant yeast cells producing polylactic acid and uses thereof
CA3024287C (en) 2016-05-19 2024-06-11 Carbios A process for degrading plastic products
JP7179002B2 (ja) 2016-12-16 2022-11-28 キャルビオス 改良されたプラスチック分解プロテアーゼ
ES2901746T3 (es) 2017-08-31 2022-03-23 Carbios Composición líquida que comprende entidades biológicas y usos de la misma
BR112020004040B1 (pt) 2017-08-31 2023-10-24 Carbiolice Artigo de poliéster biodegradável compreendendo enzimas
KR20200103757A (ko) 2017-12-21 2020-09-02 까르비오 신규한 프로테아제 및 이의 용도
CA3107560A1 (en) 2018-07-27 2020-01-30 Carbios Esterases and uses thereof
PT3830254T (pt) 2018-07-27 2025-01-24 Carbios Novas esterases e suas utilizações

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018011281A1 (en) 2016-07-12 2018-01-18 Carbios Novel esterases and uses thereof
WO2018011284A1 (en) 2016-07-12 2018-01-18 Carbios Novel esterases and uses thereof

Also Published As

Publication number Publication date
CN112673104B (zh) 2025-02-25
US20210171921A1 (en) 2021-06-10
KR20210042122A (ko) 2021-04-16
EP3830270A1 (en) 2021-06-09
US11692181B2 (en) 2023-07-04
CN112673104A (zh) 2021-04-16
JP2021531032A (ja) 2021-11-18
WO2020021117A1 (en) 2020-01-30

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