CN112673104B - 新酯酶及其用途 - Google Patents

新酯酶及其用途 Download PDF

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Publication number
CN112673104B
CN112673104B CN201980057623.3A CN201980057623A CN112673104B CN 112673104 B CN112673104 B CN 112673104B CN 201980057623 A CN201980057623 A CN 201980057623A CN 112673104 B CN112673104 B CN 112673104B
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CN
China
Prior art keywords
esterase
polyester
seq
amino acid
esterases
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Active
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CN201980057623.3A
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English (en)
Chinese (zh)
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CN112673104A (zh
Inventor
I·安德烈
V·图尼尔
A·吉勒
S·迪奎斯内
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Carbios SA
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Carbios SA
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02—Polyesters derived from dicarboxylic acids and dihydroxy compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01074—Cutinase (3.1.1.74)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/62—Plastics recycling; Rubber recycling

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Sustainable Development (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201980057623.3A 2018-07-27 2019-07-26 新酯酶及其用途 Active CN112673104B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP18306019 2018-07-27
EP18306019.3 2018-07-27
PCT/EP2019/070288 WO2020021117A1 (en) 2018-07-27 2019-07-26 Novel esterases and uses thereof

Publications (2)

Publication Number Publication Date
CN112673104A CN112673104A (zh) 2021-04-16
CN112673104B true CN112673104B (zh) 2025-02-25

Family

ID=63144938

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980057623.3A Active CN112673104B (zh) 2018-07-27 2019-07-26 新酯酶及其用途

Country Status (6)

Country Link
US (1) US11692181B2 (enExample)
EP (1) EP3830270A1 (enExample)
JP (1) JP7633149B2 (enExample)
KR (1) KR20210042122A (enExample)
CN (1) CN112673104B (enExample)
WO (1) WO2020021117A1 (enExample)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3307812B1 (en) 2015-06-12 2024-05-08 Carbios Biodegradable polyester composition and uses thereof
CA3024287C (en) 2016-05-19 2024-06-11 Carbios A process for degrading plastic products
EP3485007B1 (en) 2016-07-12 2025-10-29 Carbios Novel esterases and uses thereof
JP7179002B2 (ja) 2016-12-16 2022-11-28 キャルビオス 改良されたプラスチック分解プロテアーゼ
ES2901746T3 (es) 2017-08-31 2022-03-23 Carbios Composición líquida que comprende entidades biológicas y usos de la misma
KR20200103757A (ko) 2017-12-21 2020-09-02 까르비오 신규한 프로테아제 및 이의 용도
CA3107560A1 (en) 2018-07-27 2020-01-30 Carbios Esterases and uses thereof
PT3830254T (pt) 2018-07-27 2025-01-24 Carbios Novas esterases e suas utilizações
FR3088070B1 (fr) 2018-11-06 2021-11-26 Carbios Procede de degradation enzymatique de polyethylene terephtalate
AU2020249603A1 (en) 2019-03-28 2021-09-23 Carbios Multicomponent thermoplastic product
EP3997220B1 (en) 2019-07-11 2025-09-10 Carbios Esterases and uses thereof
EP3997219B1 (en) 2019-07-11 2025-08-06 Carbios Novel esterases and uses thereof
GB202005073D0 (en) 2020-04-06 2020-05-20 Mellizyme Biotechnology Ltd Enzymatic degradation of plastics
KR20230093468A (ko) 2020-10-27 2023-06-27 까르비오 신규한 에스테라아제 및 그 용도
DK181658B1 (en) * 2020-11-12 2024-09-11 Petroleo Brasileiro Sa Petrobras PROCESS FOR THE PRODUCTION OF GLYCOLIC ACID OR γ-HYDROXYBUTYRIC ACID INTEGRATED WITH THE BIOTECHNOLOGICAL DEPOLYMERISATION OF POLYETYLENE TEREPHTHALATE OR POLY(BUTYLENE SUCCINATE).
MX2023013755A (es) 2021-05-20 2023-12-04 Carbios Proceso para degradar un producto de plastico que comprende al menos un poliester.
CA3218310A1 (en) 2021-05-20 2022-11-24 Carbios Process for degrading a plastic product comprising at least one polyester
CN113698742B (zh) * 2021-08-31 2022-11-22 江南大学 一种聚对苯二甲酸乙二醇酯的改性方法及其在酶解过程中的应用
MX2024005891A (es) * 2021-11-16 2024-05-30 Carbios Esterasas y usos de las mismas.
EP4433582A2 (en) * 2021-11-16 2024-09-25 Carbios Esterases and uses thereof
US20250034347A1 (en) * 2021-11-16 2025-01-30 Carbios Use of esterases
WO2023242197A1 (en) 2022-06-13 2023-12-21 Carbios Process for purifying mono-ethylene glycol
CN115197927B (zh) * 2022-08-09 2024-06-21 武汉新华扬生物股份有限公司 一种降解生物基塑料的复合酶制剂及其应用
EP4321616A1 (en) 2022-08-09 2024-02-14 Covestro Deutschland AG Thermally stable carboxylic ester hydrolases in layered double hydroxides for intrinsic recyclable polymers
CN115287274B (zh) * 2022-10-08 2023-01-06 中国农业科学院生物技术研究所 角质酶iccg的突变体及其应用
WO2024151608A2 (en) 2023-01-09 2024-07-18 Protein Evolution Inc. Systems, methods, and compositions involving pretreatment and/or enzymatic degradation of crystallizable polymers, including copolymers
TW202506250A (zh) 2023-03-21 2025-02-16 美商蛋白質演化股份有限公司 從流體中分離顆粒的方法和組合物
WO2024236009A2 (en) 2023-05-15 2024-11-21 Carbios Novel esterases and uses thereof
KR20260011740A (ko) 2023-05-15 2026-01-23 까르비오 신규 에스테라제 및 이의 용도
CN117187212B (zh) * 2023-09-19 2025-02-11 源天生物科技(天津)有限公司 PET降解酶PET-mh突变体及其编码基因、重组质粒、工程菌和应用
CN117264927B (zh) * 2023-09-19 2024-11-29 源天生物科技(天津)有限公司 PET降解酶PET-mh29/124/183突变体及其编码基因、工程菌和应用
CN117448297B (zh) * 2023-09-19 2024-12-03 源天生物科技(天津)有限公司 PET降解酶PET-mh183/208突变体及其编码基因、工程菌和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012099018A1 (ja) * 2011-01-19 2012-07-26 天野エンザイム株式会社 枝葉コンポスト由来新規エステラーゼ
WO2015135757A1 (de) * 2014-03-11 2015-09-17 Henkel Ag & Co. Kgaa Pet-esterasen und deren verwendung

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2984354A1 (fr) 2011-12-20 2013-06-21 Centre Nat Rech Scient Procede de preparation d'alliage polymere/enzymes
ES2707304T3 (es) 2012-11-20 2019-04-03 Carbios Método para reciclar productos de plástico
EP3065890A2 (en) 2013-11-05 2016-09-14 Carbios A method for degrading a plastic
CA2947478C (en) 2014-05-16 2022-09-13 Carbios Process of recycling mixed pet plastic articles
TN2017000085A1 (en) 2014-10-21 2018-07-04 Carbios Polypeptide having a polyester degrading activity and uses thereof
WO2016097325A1 (en) 2014-12-19 2016-06-23 Carbios Plastic compound and preparation process
CN107532153A (zh) 2015-03-13 2018-01-02 卡比奥斯公司 具有聚酯降解活性的新多肽及其用途
EP3307812B1 (en) 2015-06-12 2024-05-08 Carbios Biodegradable polyester composition and uses thereof
US10717996B2 (en) 2015-12-21 2020-07-21 Carbios Recombinant yeast cells producing polylactic acid and uses thereof
CA3024287C (en) 2016-05-19 2024-06-11 Carbios A process for degrading plastic products
EP3485007B1 (en) * 2016-07-12 2025-10-29 Carbios Novel esterases and uses thereof
MX2019000493A (es) 2016-07-12 2019-04-01 Carbios Nuevas esterasas y usos de las mismas.
JP7179002B2 (ja) 2016-12-16 2022-11-28 キャルビオス 改良されたプラスチック分解プロテアーゼ
ES2901746T3 (es) 2017-08-31 2022-03-23 Carbios Composición líquida que comprende entidades biológicas y usos de la misma
BR112020004040B1 (pt) 2017-08-31 2023-10-24 Carbiolice Artigo de poliéster biodegradável compreendendo enzimas
KR20200103757A (ko) 2017-12-21 2020-09-02 까르비오 신규한 프로테아제 및 이의 용도
CA3107560A1 (en) 2018-07-27 2020-01-30 Carbios Esterases and uses thereof
PT3830254T (pt) 2018-07-27 2025-01-24 Carbios Novas esterases e suas utilizações

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012099018A1 (ja) * 2011-01-19 2012-07-26 天野エンザイム株式会社 枝葉コンポスト由来新規エステラーゼ
WO2015135757A1 (de) * 2014-03-11 2015-09-17 Henkel Ag & Co. Kgaa Pet-esterasen und deren verwendung

Also Published As

Publication number Publication date
US20210171921A1 (en) 2021-06-10
KR20210042122A (ko) 2021-04-16
EP3830270A1 (en) 2021-06-09
JP7633149B2 (ja) 2025-02-19
US11692181B2 (en) 2023-07-04
CN112673104A (zh) 2021-04-16
JP2021531032A (ja) 2021-11-18
WO2020021117A1 (en) 2020-01-30

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