JP7601756B2 - 新規なエステラーゼ及びその使用 - Google Patents

新規なエステラーゼ及びその使用 Download PDF

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Publication number
JP7601756B2
JP7601756B2 JP2021504395A JP2021504395A JP7601756B2 JP 7601756 B2 JP7601756 B2 JP 7601756B2 JP 2021504395 A JP2021504395 A JP 2021504395A JP 2021504395 A JP2021504395 A JP 2021504395A JP 7601756 B2 JP7601756 B2 JP 7601756B2
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JP
Japan
Prior art keywords
esterase
seq
polyester
amino acid
present
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Active
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JP2021504395A
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English (en)
Japanese (ja)
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JP2021531031A5 (enExample
JP2021531031A (ja
Inventor
ドゥケーヌ,ソフィー
トゥルニエ,ヴァンサン
マルティ,アラン
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Carbios SA
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Carbios SA
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Publication of JP2021531031A5 publication Critical patent/JP2021531031A5/ja
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46Hydrolases (3)
    • A61K38/465Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00Recovery or working-up of waste materials
    • C08J11/04Recovery or working-up of waste materials of polymers
    • C08J11/10Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/01Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01074Cutinase (3.1.1.74)
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02Polyesters derived from dicarboxylic acids and dihydroxy compounds
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/50Reuse, recycling or recovery technologies
    • Y02W30/62Plastics recycling; Rubber recycling

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Epidemiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
JP2021504395A 2018-07-27 2019-07-26 新規なエステラーゼ及びその使用 Active JP7601756B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP18306020.1 2018-07-27
EP18306020 2018-07-27
EP18306447 2018-11-05
EP18306447.6 2018-11-05
PCT/EP2019/070289 WO2020021118A1 (en) 2018-07-27 2019-07-26 Novel esterases and uses thereof

Publications (3)

Publication Number Publication Date
JP2021531031A JP2021531031A (ja) 2021-11-18
JP2021531031A5 JP2021531031A5 (enExample) 2022-08-02
JP7601756B2 true JP7601756B2 (ja) 2024-12-17

Family

ID=67396962

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2021504395A Active JP7601756B2 (ja) 2018-07-27 2019-07-26 新規なエステラーゼ及びその使用

Country Status (8)

Country Link
US (1) US11851689B2 (enExample)
EP (1) EP3830271A1 (enExample)
JP (1) JP7601756B2 (enExample)
KR (1) KR102876883B1 (enExample)
CN (2) CN120098957A (enExample)
CA (1) CA3107560A1 (enExample)
MX (1) MX2021001045A (enExample)
WO (1) WO2020021118A1 (enExample)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3307812B1 (en) 2015-06-12 2024-05-08 Carbios Biodegradable polyester composition and uses thereof
CA3024287C (en) 2016-05-19 2024-06-11 Carbios A process for degrading plastic products
EP3485007B1 (en) 2016-07-12 2025-10-29 Carbios Novel esterases and uses thereof
JP7179002B2 (ja) 2016-12-16 2022-11-28 キャルビオス 改良されたプラスチック分解プロテアーゼ
ES2901746T3 (es) 2017-08-31 2022-03-23 Carbios Composición líquida que comprende entidades biológicas y usos de la misma
KR20200103757A (ko) 2017-12-21 2020-09-02 까르비오 신규한 프로테아제 및 이의 용도
PT3830254T (pt) 2018-07-27 2025-01-24 Carbios Novas esterases e suas utilizações
CN112673104B (zh) 2018-07-27 2025-02-25 卡比奥斯公司 新酯酶及其用途
FR3088070B1 (fr) * 2018-11-06 2021-11-26 Carbios Procede de degradation enzymatique de polyethylene terephtalate
AU2020249603A1 (en) 2019-03-28 2021-09-23 Carbios Multicomponent thermoplastic product
EP3997220B1 (en) 2019-07-11 2025-09-10 Carbios Esterases and uses thereof
EP3997219B1 (en) 2019-07-11 2025-08-06 Carbios Novel esterases and uses thereof
GB202005073D0 (en) 2020-04-06 2020-05-20 Mellizyme Biotechnology Ltd Enzymatic degradation of plastics
KR20230093468A (ko) 2020-10-27 2023-06-27 까르비오 신규한 에스테라아제 및 그 용도
MX2023013755A (es) 2021-05-20 2023-12-04 Carbios Proceso para degradar un producto de plastico que comprende al menos un poliester.
CA3218310A1 (en) 2021-05-20 2022-11-24 Carbios Process for degrading a plastic product comprising at least one polyester
EP4370534A1 (en) 2021-07-13 2024-05-22 Novozymes A/S Recombinant cutinase expression
MX2024001814A (es) * 2021-08-11 2024-03-01 Biometis Tech Inc Degradacion enzimatica de polietilentereftalato.
MX2024005891A (es) * 2021-11-16 2024-05-30 Carbios Esterasas y usos de las mismas.
US20250034347A1 (en) * 2021-11-16 2025-01-30 Carbios Use of esterases
EP4433582A2 (en) * 2021-11-16 2024-09-25 Carbios Esterases and uses thereof
CN114891767B (zh) * 2022-05-19 2023-04-28 华南理工大学 角质酶-酯酶融合蛋白及其应用
WO2023242197A1 (en) 2022-06-13 2023-12-21 Carbios Process for purifying mono-ethylene glycol
EP4321616A1 (en) 2022-08-09 2024-02-14 Covestro Deutschland AG Thermally stable carboxylic ester hydrolases in layered double hydroxides for intrinsic recyclable polymers
KR20260011740A (ko) 2023-05-15 2026-01-23 까르비오 신규 에스테라제 및 이의 용도
WO2024236009A2 (en) 2023-05-15 2024-11-21 Carbios Novel esterases and uses thereof
IT202300014223A1 (it) * 2023-07-07 2025-01-07 Milano Politecnico Enzima ingegnerizzato termostabile
WO2025128934A1 (en) * 2023-12-15 2025-06-19 Flagship Pioneering Innovations Vii, Llc Esterases and related methods

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012099018A1 (ja) 2011-01-19 2012-07-26 天野エンザイム株式会社 枝葉コンポスト由来新規エステラーゼ
WO2015085920A1 (en) 2013-12-11 2015-06-18 Novozymes A/S Cutinase variants and polynucleotides encoding same
WO2018011281A1 (en) 2016-07-12 2018-01-18 Carbios Novel esterases and uses thereof
WO2018011284A1 (en) 2016-07-12 2018-01-18 Carbios Novel esterases and uses thereof

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2984354A1 (fr) 2011-12-20 2013-06-21 Centre Nat Rech Scient Procede de preparation d'alliage polymere/enzymes
ES2707304T3 (es) 2012-11-20 2019-04-03 Carbios Método para reciclar productos de plástico
EP3065890A2 (en) 2013-11-05 2016-09-14 Carbios A method for degrading a plastic
CA2947478C (en) 2014-05-16 2022-09-13 Carbios Process of recycling mixed pet plastic articles
TN2017000085A1 (en) 2014-10-21 2018-07-04 Carbios Polypeptide having a polyester degrading activity and uses thereof
WO2016097325A1 (en) 2014-12-19 2016-06-23 Carbios Plastic compound and preparation process
CN107532153A (zh) 2015-03-13 2018-01-02 卡比奥斯公司 具有聚酯降解活性的新多肽及其用途
EP3307812B1 (en) 2015-06-12 2024-05-08 Carbios Biodegradable polyester composition and uses thereof
US10717996B2 (en) 2015-12-21 2020-07-21 Carbios Recombinant yeast cells producing polylactic acid and uses thereof
CA3024287C (en) 2016-05-19 2024-06-11 Carbios A process for degrading plastic products
JP7179002B2 (ja) 2016-12-16 2022-11-28 キャルビオス 改良されたプラスチック分解プロテアーゼ
ES2901746T3 (es) 2017-08-31 2022-03-23 Carbios Composición líquida que comprende entidades biológicas y usos de la misma
BR112020004040B1 (pt) 2017-08-31 2023-10-24 Carbiolice Artigo de poliéster biodegradável compreendendo enzimas
KR20200103757A (ko) 2017-12-21 2020-09-02 까르비오 신규한 프로테아제 및 이의 용도
PT3830254T (pt) 2018-07-27 2025-01-24 Carbios Novas esterases e suas utilizações
CN112673104B (zh) 2018-07-27 2025-02-25 卡比奥斯公司 新酯酶及其用途

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012099018A1 (ja) 2011-01-19 2012-07-26 天野エンザイム株式会社 枝葉コンポスト由来新規エステラーゼ
WO2015085920A1 (en) 2013-12-11 2015-06-18 Novozymes A/S Cutinase variants and polynucleotides encoding same
WO2018011281A1 (en) 2016-07-12 2018-01-18 Carbios Novel esterases and uses thereof
WO2018011284A1 (en) 2016-07-12 2018-01-18 Carbios Novel esterases and uses thereof

Also Published As

Publication number Publication date
CN120098957A (zh) 2025-06-06
US11851689B2 (en) 2023-12-26
CN112639101A (zh) 2021-04-09
JP2021531031A (ja) 2021-11-18
US20210180037A1 (en) 2021-06-17
CA3107560A1 (en) 2020-01-30
CN112639101B (zh) 2025-03-18
KR20210043599A (ko) 2021-04-21
EP3830271A1 (en) 2021-06-09
MX2021001045A (es) 2021-04-12
KR102876883B1 (ko) 2025-10-27
WO2020021118A1 (en) 2020-01-30

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