JP7561364B2 - 蛍光偏光免疫分析法 - Google Patents

蛍光偏光免疫分析法 Download PDF

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JP7561364B2
JP7561364B2 JP2020080767A JP2020080767A JP7561364B2 JP 7561364 B2 JP7561364 B2 JP 7561364B2 JP 2020080767 A JP2020080767 A JP 2020080767A JP 2020080767 A JP2020080767 A JP 2020080767A JP 7561364 B2 JP7561364 B2 JP 7561364B2
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fluorescence polarization
substance
antibody
fluorescence
measured
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JP2021173731A (ja
Inventor
真央 福山
彰秀 火原
阿由子 今井
幸治 重村
学 渡慶次
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Tianma Japan Ltd
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Tianma Japan Ltd
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Priority to JP2020080767A priority Critical patent/JP7561364B2/ja
Priority to CN202511485711.7A priority patent/CN121208351A/zh
Priority to US17/241,861 priority patent/US20210341488A1/en
Priority to CN202110458846.XA priority patent/CN113588961A/zh
Publication of JP2021173731A publication Critical patent/JP2021173731A/ja
Priority to JP2024110033A priority patent/JP7837518B2/ja
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7786—Fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/062—LED's

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
JP2020080767A 2020-04-30 2020-04-30 蛍光偏光免疫分析法 Active JP7561364B2 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2020080767A JP7561364B2 (ja) 2020-04-30 2020-04-30 蛍光偏光免疫分析法
CN202511485711.7A CN121208351A (zh) 2020-04-30 2021-04-27 荧光偏振免疫分析和荧光标记物质
US17/241,861 US20210341488A1 (en) 2020-04-30 2021-04-27 Fluorescence polarization immunoassay and fluorescent labeling substance
CN202110458846.XA CN113588961A (zh) 2020-04-30 2021-04-27 荧光偏振免疫分析和荧光标记物质
JP2024110033A JP7837518B2 (ja) 2020-04-30 2024-07-09 蛍光偏光免疫分析法

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JP2020080767A JP7561364B2 (ja) 2020-04-30 2020-04-30 蛍光偏光免疫分析法

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JP7561364B2 true JP7561364B2 (ja) 2024-10-04

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Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12566139B2 (en) 2022-05-13 2026-03-03 Canon Kabushiki Kaisha Method analysis employing measurement based on polarization anisotropy
CN115326761B (zh) * 2022-06-28 2025-03-14 中国水产科学研究院南海水产研究所 一种用于检测丁香酚残留量的试剂盒及其应用
JP2024049691A (ja) * 2022-09-29 2024-04-10 Ｔｉａｎｍａ Ｊａｐａｎ株式会社 蛍光偏光免疫測定法及び蛍光偏光免疫測定装置
EP4646582B1 (en) * 2023-03-21 2026-02-18 Bit Group France Method of fluorescent dye variation compensation by free state fluorescence measurement
CN117929727B (zh) * 2023-12-28 2024-09-06 南京迪安医学检验所有限公司 一种乙肝病毒检测试剂盒及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003081243A1 (en) 2002-03-27 2003-10-02 Matsushita Electric Industrial Co., Ltd. Fluorescent polarization method, kit used therefor and biosensor
US20090142853A1 (en) 2005-08-11 2009-06-04 Eksigent Technologies, Llc Microfluidic system and methods
JP2019503477A (ja) 2015-12-18 2019-02-07 バリタセル リミテッド 試料中の標的分子の存在量を決定する方法
JP2020503062A (ja) 2016-11-07 2020-01-30 クロスベータ バイオサイエンシズ べスローテン フェンノートシャップ 新規アミロイドベータオリゴマー特異的結合分子

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US5445935A (en) * 1992-11-23 1995-08-29 Royer; Catherine A. Quantitative detection of macromolecules with fluorescent oligonucleotides
JP3255293B2 (ja) * 1997-09-05 2002-02-12 松下電器産業株式会社 蛍光偏光法
US20060086309A1 (en) * 2002-06-24 2006-04-27 Fluiding Corporation Recirculating fluidic network and methods for using the same
AU2004228678A1 (en) * 2003-04-03 2004-10-21 Fluidigm Corp. Microfluidic devices and methods of using same
CA2538038C (en) * 2003-09-11 2015-10-27 Theranos, Inc. Medical device for analyte monitoring and drug delivery
US8497361B2 (en) * 2010-08-23 2013-07-30 Sri International Topoisomerase binding probe and method of use
CN104066435A (zh) * 2011-12-21 2014-09-24 纽约大学 蛋白水解抗性的氢键替代螺旋
WO2016077840A2 (en) * 2014-11-14 2016-05-19 Ossianix, Inc. TfR SELECTIVE BINDING COMPOUNDS AND RELATED METHODS
GB201510758D0 (en) * 2015-06-18 2015-08-05 Ucb Biopharma Sprl Novel TNFa structure for use in therapy
MA43219A (fr) * 2015-11-10 2018-09-19 Univ Medical Center Hamburg Eppendorf Polypeptides de liaison d'antigène dirigés contre cd38

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003081243A1 (en) 2002-03-27 2003-10-02 Matsushita Electric Industrial Co., Ltd. Fluorescent polarization method, kit used therefor and biosensor
US20090142853A1 (en) 2005-08-11 2009-06-04 Eksigent Technologies, Llc Microfluidic system and methods
JP2019503477A (ja) 2015-12-18 2019-02-07 バリタセル リミテッド 試料中の標的分子の存在量を決定する方法
JP2020503062A (ja) 2016-11-07 2020-01-30 クロスベータ バイオサイエンシズ べスローテン フェンノートシャップ 新規アミロイドベータオリゴマー特異的結合分子

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JP7837518B2 (ja) 2026-03-31
US20210341488A1 (en) 2021-11-04
CN113588961A (zh) 2021-11-02
JP2024124545A (ja) 2024-09-12
JP2021173731A (ja) 2021-11-01
CN121208351A (zh) 2025-12-26

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