CN121208351A - 荧光偏振免疫分析和荧光标记物质 - Google Patents

荧光偏振免疫分析和荧光标记物质

Info

Publication number
CN121208351A
CN121208351A CN202511485711.7A CN202511485711A CN121208351A CN 121208351 A CN121208351 A CN 121208351A CN 202511485711 A CN202511485711 A CN 202511485711A CN 121208351 A CN121208351 A CN 121208351A
Authority
CN
China
Prior art keywords
fluorescence polarization
antibody
fluorescent
substance
fluorescent dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202511485711.7A
Other languages
English (en)
Chinese (zh)
Inventor
福山真央
火原彰秀
今井阿由子
重村幸治
渡庆次学
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tohoku University NUC
Hokkaido University NUC
Tianma Japan Ltd
Original Assignee
Tohoku University NUC
Hokkaido University NUC
Tianma Japan Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tohoku University NUC, Hokkaido University NUC, Tianma Japan Ltd filed Critical Tohoku University NUC
Publication of CN121208351A publication Critical patent/CN121208351A/zh
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7786—Fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/062—LED's

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CN202511485711.7A 2020-04-30 2021-04-27 荧光偏振免疫分析和荧光标记物质 Pending CN121208351A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020-080767 2020-04-30
JP2020080767A JP7561364B2 (ja) 2020-04-30 2020-04-30 蛍光偏光免疫分析法
CN202110458846.XA CN113588961A (zh) 2020-04-30 2021-04-27 荧光偏振免疫分析和荧光标记物质

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN202110458846.XA Division CN113588961A (zh) 2020-04-30 2021-04-27 荧光偏振免疫分析和荧光标记物质

Publications (1)

Publication Number Publication Date
CN121208351A true CN121208351A (zh) 2025-12-26

Family

ID=78243117

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202511485711.7A Pending CN121208351A (zh) 2020-04-30 2021-04-27 荧光偏振免疫分析和荧光标记物质
CN202110458846.XA Pending CN113588961A (zh) 2020-04-30 2021-04-27 荧光偏振免疫分析和荧光标记物质

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202110458846.XA Pending CN113588961A (zh) 2020-04-30 2021-04-27 荧光偏振免疫分析和荧光标记物质

Country Status (3)

Country Link
US (1) US20210341488A1 (https=)
JP (2) JP7561364B2 (https=)
CN (2) CN121208351A (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12566139B2 (en) 2022-05-13 2026-03-03 Canon Kabushiki Kaisha Method analysis employing measurement based on polarization anisotropy
CN115326761B (zh) * 2022-06-28 2025-03-14 中国水产科学研究院南海水产研究所 一种用于检测丁香酚残留量的试剂盒及其应用
JP2024049691A (ja) * 2022-09-29 2024-04-10 Ｔｉａｎｍａ Ｊａｐａｎ株式会社 蛍光偏光免疫測定法及び蛍光偏光免疫測定装置
EP4646582B1 (en) * 2023-03-21 2026-02-18 Bit Group France Method of fluorescent dye variation compensation by free state fluorescence measurement
CN117929727B (zh) * 2023-12-28 2024-09-06 南京迪安医学检验所有限公司 一种乙肝病毒检测试剂盒及其应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5445935A (en) * 1992-11-23 1995-08-29 Royer; Catherine A. Quantitative detection of macromolecules with fluorescent oligonucleotides
JP3255293B2 (ja) * 1997-09-05 2002-02-12 松下電器産業株式会社 蛍光偏光法
WO2003081243A1 (en) 2002-03-27 2003-10-02 Matsushita Electric Industrial Co., Ltd. Fluorescent polarization method, kit used therefor and biosensor
US20060086309A1 (en) * 2002-06-24 2006-04-27 Fluiding Corporation Recirculating fluidic network and methods for using the same
AU2004228678A1 (en) * 2003-04-03 2004-10-21 Fluidigm Corp. Microfluidic devices and methods of using same
CA2538038C (en) * 2003-09-11 2015-10-27 Theranos, Inc. Medical device for analyte monitoring and drug delivery
WO2007021813A2 (en) 2005-08-11 2007-02-22 Eksigent Technologies, Llc Microfluidic system and methods
US8497361B2 (en) * 2010-08-23 2013-07-30 Sri International Topoisomerase binding probe and method of use
CN104066435A (zh) * 2011-12-21 2014-09-24 纽约大学 蛋白水解抗性的氢键替代螺旋
WO2016077840A2 (en) * 2014-11-14 2016-05-19 Ossianix, Inc. TfR SELECTIVE BINDING COMPOUNDS AND RELATED METHODS
GB201510758D0 (en) * 2015-06-18 2015-08-05 Ucb Biopharma Sprl Novel TNFa structure for use in therapy
MA43219A (fr) * 2015-11-10 2018-09-19 Univ Medical Center Hamburg Eppendorf Polypeptides de liaison d'antigène dirigés contre cd38
US20190011437A1 (en) 2015-12-18 2019-01-10 Valitacell Limited A method of determining the abundance of a target molecule in a sample
CN110325547B (zh) 2016-11-07 2023-12-26 德格克斯有限公司 新淀粉样蛋白β寡聚体特异性结合分子

Also Published As

Publication number Publication date
JP7837518B2 (ja) 2026-03-31
US20210341488A1 (en) 2021-11-04
CN113588961A (zh) 2021-11-02
JP2024124545A (ja) 2024-09-12
JP2021173731A (ja) 2021-11-01
JP7561364B2 (ja) 2024-10-04

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