JP7469317B2 - ヒト多能性幹細胞からの背側由来オリゴデンドロサイト前駆細胞 - Google Patents

ヒト多能性幹細胞からの背側由来オリゴデンドロサイト前駆細胞 Download PDF

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JP7469317B2
JP7469317B2 JP2021543274A JP2021543274A JP7469317B2 JP 7469317 B2 JP7469317 B2 JP 7469317B2 JP 2021543274 A JP2021543274 A JP 2021543274A JP 2021543274 A JP2021543274 A JP 2021543274A JP 7469317 B2 JP7469317 B2 JP 7469317B2
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憲人 大西
ネイサン シー． マンレイ
クレイグ アール． ハルバーシュタット
エリック エム． ウィットリー
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アステリアス バイオセラピューティクス インコーポレイテッド
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JP2021543274A 2019-01-23 2020-01-23 ヒト多能性幹細胞からの背側由来オリゴデンドロサイト前駆細胞 Active JP7469317B2 (ja)

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KR (2) KR20260020476A (https=)
CN (2) CN113474451B (https=)
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Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116121189A (zh) 2016-03-30 2023-05-16 阿斯特利亚斯生物治疗股份公司 少突胶质细胞祖细胞组合物
IL281628B2 (en) 2018-09-19 2025-06-01 Lineage Cell Therapeutics Inc Methods for differentiating pluripotent stem cells in dynamic suspension culture
WO2020154533A1 (en) 2019-01-23 2020-07-30 Asterias Biotherapeutics, Inc. Dorsally-derived oligodendrocyte progenitor cells from human pluripotent stem cells
AU2022212124A1 (en) * 2021-01-28 2023-08-10 Asterias Biotherapeutics, Inc. Compositions and methods for treating spinal cord injuries
IL305737A (en) * 2021-03-10 2023-11-01 Lineage Cell Therapeutics Inc Methods for generating oligodendrocyte progenitor cells and their use
WO2023147512A2 (en) * 2022-01-28 2023-08-03 Asterias Biotherapeutics, Inc. Compositions and methods for treating spinal cord injuries
CA3256595A1 (en) * 2022-05-24 2023-11-30 Bluerock Therapeutics Lp METHODS FOR MANUFACTURED OLIGODENDROCYTE PROGENITOR CELLS
WO2025049991A1 (en) * 2023-08-31 2025-03-06 The Board Of Trustees Of The Leland Stanford Junior University Compositions comprising neurons and methods of using the same
KR20250094234A (ko) * 2023-12-18 2025-06-25 ㈜바이오솔빅스 콘케이브와 진탕배양 기법으로 형성된 전분화능 줄기세포 유래 신경세포 제조방법 및 이에 의해 제조된 3d 신경 오가노이드를 통한 약물 독성 평가방법

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006517084A (ja) 2002-07-16 2006-07-20 トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク 胚性幹細胞の分化を誘導する方法及びその用途
JP2010536357A (ja) 2007-08-20 2010-12-02 ユニヴァルシテ リブレ デ ブリュッセル 多能性幹細胞からのニューロン細胞の生成
JP2017511153A (ja) 2014-03-21 2017-04-20 セルラー ダイナミクス インターナショナル， インコーポレイテッド 中脳ドーパミン作動性ニューロンの生産およびその使用方法
WO2017132596A1 (en) 2016-01-27 2017-08-03 Memorial Sloan-Kettering Cancer Center Differentiation of cortical neurons from human pluripotent stem cells
WO2020061371A2 (en) 2018-09-19 2020-03-26 Lineage Cell Therapeutics, Inc. Methods for differentiating pluripotent stem cells in dynamic suspension culture

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998048001A1 (en) * 1997-04-24 1998-10-29 California Institute Of Technology Methods for differentiating neural stem cells
US6361996B1 (en) * 1997-05-07 2002-03-26 University Of Utah Research Foundation Neuroepithelial stem cells and glial-restricted intermediate precursors
AU729377B2 (en) 1997-10-23 2001-02-01 Asterias Biotherapeutics, Inc. Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
CA2388736A1 (en) 1999-10-22 2001-04-26 Washington University Oligodendrocyte cell cultures and methods for their preparation and use
US6280718B1 (en) 1999-11-08 2001-08-28 Wisconsin Alumni Reasearch Foundation Hematopoietic differentiation of human pluripotent embryonic stem cells
CA2403000C (en) 2000-03-14 2015-06-23 Es Cell International Pte Ltd Embryonic stem cells and neural progenitor cells derived therefrom
AU2003211063A1 (en) 2002-02-15 2003-09-09 Cornell Research Foundation, Inc. Myelination of congenitally dysmyelinated forebrains using oligodendrocyte progenitor cells
US7285415B2 (en) 2002-07-11 2007-10-23 The Regents Of The University Of California Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
US20050101014A1 (en) 2002-07-11 2005-05-12 Keirstead Hans S. Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
US20050031598A1 (en) 2002-12-10 2005-02-10 Shulamit Levenberg Engineering three-dimensional tissue structures using differentiating embryonic stem cells
EP2399990B1 (en) 2003-06-27 2015-07-22 DePuy Synthes Products, Inc. Cells derived from post-partum umbilical cord for use in treatment of disease of the heart and circulatory system
CN101084303A (zh) 2004-11-17 2007-12-05 神经干公司 用于治疗神经变性症状的人类神经细胞的移植
US8709797B2 (en) 2006-06-20 2014-04-29 Cook General Biotechnology Llc Systems and methods for cryopreservation of cells
WO2009008928A2 (en) 2007-04-13 2009-01-15 Stemnion, Inc. Methods for treating nervous system injury and disease
KR100941036B1 (ko) 2007-10-05 2010-02-05 고려대학교 산학협력단 인간 배아줄기세포로부터 척수신경계 희소돌기 아교세포생산을 위한 삼단계 분화기법
EP2217252A2 (en) 2007-10-29 2010-08-18 Hadasit Medical Research Services & Development Limited Human stem cell-derived neural precursors for treatment of autoimmune diseases of the central nervous system
US8227247B2 (en) 2007-12-20 2012-07-24 Wisconsin Alumni Research Foundation Method of generating myelinating oligodendrocytes
CA2714010C (en) 2008-01-30 2020-06-16 Corning Incorporated Synthetic surfaces for culturing stem cell derived oligodendrocyte progenitor cells
KR20110011658A (ko) 2008-05-08 2011-02-08 유니버시티 오브 로체스터 최적화된 세포 제제에 의한 수초 질환의 치료
CN102083964B (zh) 2008-05-09 2013-06-12 格拉斯哥大学大学行政评议会 与基于细胞的疗法相关的材料和方法
US20100158878A1 (en) 2008-12-23 2010-06-24 Alexandra Capela Target populations of oligodendrocyte precursor cells and methods of making and using same
CN102803472B (zh) 2009-06-25 2014-04-23 吉隆公司 去除了外来表型的由多能干细胞分化的子代细胞
WO2011059920A2 (en) 2009-11-10 2011-05-19 The J. David Gladstone Institutes Methods of generating neural stem cells
CN102160546B (zh) 2010-02-24 2016-01-20 库克通用生物科技有限责任公司 冷冻保存细胞的系统和方法
WO2012021818A2 (en) 2010-08-13 2012-02-16 Georgetown University Ggf2 and methods of use
CN103429734A (zh) 2010-10-26 2013-12-04 卡斯西部储备大学 用于产生神经胶质细胞的群体的分化方法
CN103384680B (zh) 2010-12-17 2015-08-26 拜奥拉米那公司 细胞培养基
TWI586805B (zh) 2011-01-12 2017-06-11 城戶常雄 獲得及維持在體外易於分化為寡樹突細胞－族系細胞之哺乳類神經幹細胞及／或神經祖源細胞的純或增富族群之培養方法
ES2666503T3 (es) 2012-05-16 2018-05-04 Becton, Dickinson And Company Características distintivas de superficie celular para aislar neuronas de cultivos celulares derivados de células madre pluripotentes
EP2954046B1 (en) 2013-02-06 2025-10-15 University Of Rochester Induced pluripotent cell-derived oligodendrocyte progenitor cells for the treatment of myelin disorders
US10160950B2 (en) 2013-03-01 2018-12-25 Wisconsin Alumni Research Foundation Methods of maintaining, expanding and differentiating neuronal subtype specific progenitors
KR20230058556A (ko) 2013-03-15 2023-05-03 아스텔라스 인스티튜트 포 리제너러티브 메디슨 만능 줄기 세포로부터 제조된 광수용기 및 광수용기 전구체
WO2015088625A2 (en) 2013-09-25 2015-06-18 Case Western Reserve University Compounds and methods of promoting myelination
KR102585909B1 (ko) 2014-05-22 2023-10-05 뉴욕 스템 셀 파운데이션, 인코포레이티드 다능성 줄기 세포로부터 유래된 기능성 희소돌기아교세포 및 이의 제조 및 사용 방법
WO2016063985A1 (ja) * 2014-10-24 2016-04-28 大日本住友製薬株式会社 神経組織の製造方法
CN105624116B (zh) * 2014-11-07 2020-04-07 中国科学院上海生命科学研究院 利用多能干细胞制备神经前体细胞的方法
WO2016103269A1 (en) 2014-12-23 2016-06-30 Ramot At Tel-Aviv University Ltd. Populations of neural progenitor cells and methods of producing and using same
US10286009B2 (en) 2015-05-16 2019-05-14 Asterias Biotherapeutics, Inc. Pluripotent stem cell-derived oligodendrocyte progenitor cells for the treatment of spinal cord injury
CN116121189A (zh) 2016-03-30 2023-05-16 阿斯特利亚斯生物治疗股份公司 少突胶质细胞祖细胞组合物
WO2020154533A1 (en) 2019-01-23 2020-07-30 Asterias Biotherapeutics, Inc. Dorsally-derived oligodendrocyte progenitor cells from human pluripotent stem cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006517084A (ja) 2002-07-16 2006-07-20 トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク 胚性幹細胞の分化を誘導する方法及びその用途
JP2010536357A (ja) 2007-08-20 2010-12-02 ユニヴァルシテ リブレ デ ブリュッセル 多能性幹細胞からのニューロン細胞の生成
JP2017511153A (ja) 2014-03-21 2017-04-20 セルラー ダイナミクス インターナショナル， インコーポレイテッド 中脳ドーパミン作動性ニューロンの生産およびその使用方法
WO2017132596A1 (en) 2016-01-27 2017-08-03 Memorial Sloan-Kettering Cancer Center Differentiation of cortical neurons from human pluripotent stem cells
WO2020061371A2 (en) 2018-09-19 2020-03-26 Lineage Cell Therapeutics, Inc. Methods for differentiating pluripotent stem cells in dynamic suspension culture

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Develop. Neurobiol.，2012年，Vol.72，pp.1471-1481
Methods，2018年，Vol.133，pp.65-80
Stem Cell Res. Ther.，2018年，Vol.9，67 (pp.1-13)
Stem Cells Development，2014年，Vol.23, No.1，pp.5-15

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