CN113474451B - 来自人多能干细胞的背源性少突胶质祖细胞 - Google Patents

来自人多能干细胞的背源性少突胶质祖细胞 Download PDF

Info

Publication number
CN113474451B
CN113474451B CN202080016840.0A CN202080016840A CN113474451B CN 113474451 B CN113474451 B CN 113474451B CN 202080016840 A CN202080016840 A CN 202080016840A CN 113474451 B CN113474451 B CN 113474451B
Authority
CN
China
Prior art keywords
cells
pluripotent stem
cell
opc
progenitor cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202080016840.0A
Other languages
English (en)
Chinese (zh)
Other versions
CN113474451A (zh
Inventor
K·奥尼施
N·C·曼雷
C·R·哈尔贝施塔特
E·M·怀特利
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Astrias Biotherapy Co ltd
Original Assignee
Astrias Biotherapy Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Astrias Biotherapy Co ltd filed Critical Astrias Biotherapy Co ltd
Priority to CN202510214051.2A priority Critical patent/CN120060141A/zh
Publication of CN113474451A publication Critical patent/CN113474451A/zh
Application granted granted Critical
Publication of CN113474451B publication Critical patent/CN113474451B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0622—Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/113—Acidic fibroblast growth factor (aFGF, FGF-1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/03—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from non-embryonic pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Neurology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Neurosurgery (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
CN202080016840.0A 2019-01-23 2020-01-23 来自人多能干细胞的背源性少突胶质祖细胞 Active CN113474451B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202510214051.2A CN120060141A (zh) 2019-01-23 2020-01-23 来自人多能干细胞的背源性少突胶质祖细胞

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962796077P 2019-01-23 2019-01-23
US62/796,077 2019-01-23
PCT/US2020/014834 WO2020154533A1 (en) 2019-01-23 2020-01-23 Dorsally-derived oligodendrocyte progenitor cells from human pluripotent stem cells

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202510214051.2A Division CN120060141A (zh) 2019-01-23 2020-01-23 来自人多能干细胞的背源性少突胶质祖细胞

Publications (2)

Publication Number Publication Date
CN113474451A CN113474451A (zh) 2021-10-01
CN113474451B true CN113474451B (zh) 2025-06-10

Family

ID=71609769

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202080016840.0A Active CN113474451B (zh) 2019-01-23 2020-01-23 来自人多能干细胞的背源性少突胶质祖细胞
CN202510214051.2A Pending CN120060141A (zh) 2019-01-23 2020-01-23 来自人多能干细胞的背源性少突胶质祖细胞

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202510214051.2A Pending CN120060141A (zh) 2019-01-23 2020-01-23 来自人多能干细胞的背源性少突胶质祖细胞

Country Status (9)

Country Link
US (2) US11603518B2 (https=)
EP (1) EP3914264A4 (https=)
JP (2) JP7469317B2 (https=)
KR (2) KR20260020476A (https=)
CN (2) CN113474451B (https=)
AU (1) AU2020210757B2 (https=)
CA (1) CA3127213A1 (https=)
SG (1) SG11202107732XA (https=)
WO (1) WO2020154533A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116121189A (zh) 2016-03-30 2023-05-16 阿斯特利亚斯生物治疗股份公司 少突胶质细胞祖细胞组合物
IL281628B2 (en) 2018-09-19 2025-06-01 Lineage Cell Therapeutics Inc Methods for differentiating pluripotent stem cells in dynamic suspension culture
WO2020154533A1 (en) 2019-01-23 2020-07-30 Asterias Biotherapeutics, Inc. Dorsally-derived oligodendrocyte progenitor cells from human pluripotent stem cells
AU2022212124A1 (en) * 2021-01-28 2023-08-10 Asterias Biotherapeutics, Inc. Compositions and methods for treating spinal cord injuries
IL305737A (en) * 2021-03-10 2023-11-01 Lineage Cell Therapeutics Inc Methods for generating oligodendrocyte progenitor cells and their use
WO2023147512A2 (en) * 2022-01-28 2023-08-03 Asterias Biotherapeutics, Inc. Compositions and methods for treating spinal cord injuries
CA3256595A1 (en) * 2022-05-24 2023-11-30 Bluerock Therapeutics Lp METHODS FOR MANUFACTURED OLIGODENDROCYTE PROGENITOR CELLS
WO2025049991A1 (en) * 2023-08-31 2025-03-06 The Board Of Trustees Of The Leland Stanford Junior University Compositions comprising neurons and methods of using the same
KR20250094234A (ko) * 2023-12-18 2025-06-25 ㈜바이오솔빅스 콘케이브와 진탕배양 기법으로 형성된 전분화능 줄기세포 유래 신경세포 제조방법 및 이에 의해 제조된 3d 신경 오가노이드를 통한 약물 독성 평가방법

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004007665A2 (en) * 2002-07-16 2004-01-22 Trustees Of Columbia University In The City Of Newyork Methods for inducing differentiation of embryonic stem cells and uses thereof

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998048001A1 (en) * 1997-04-24 1998-10-29 California Institute Of Technology Methods for differentiating neural stem cells
US6361996B1 (en) * 1997-05-07 2002-03-26 University Of Utah Research Foundation Neuroepithelial stem cells and glial-restricted intermediate precursors
AU729377B2 (en) 1997-10-23 2001-02-01 Asterias Biotherapeutics, Inc. Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
CA2388736A1 (en) 1999-10-22 2001-04-26 Washington University Oligodendrocyte cell cultures and methods for their preparation and use
US6280718B1 (en) 1999-11-08 2001-08-28 Wisconsin Alumni Reasearch Foundation Hematopoietic differentiation of human pluripotent embryonic stem cells
CA2403000C (en) 2000-03-14 2015-06-23 Es Cell International Pte Ltd Embryonic stem cells and neural progenitor cells derived therefrom
AU2003211063A1 (en) 2002-02-15 2003-09-09 Cornell Research Foundation, Inc. Myelination of congenitally dysmyelinated forebrains using oligodendrocyte progenitor cells
US7285415B2 (en) 2002-07-11 2007-10-23 The Regents Of The University Of California Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
US20050101014A1 (en) 2002-07-11 2005-05-12 Keirstead Hans S. Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
US20050031598A1 (en) 2002-12-10 2005-02-10 Shulamit Levenberg Engineering three-dimensional tissue structures using differentiating embryonic stem cells
EP2399990B1 (en) 2003-06-27 2015-07-22 DePuy Synthes Products, Inc. Cells derived from post-partum umbilical cord for use in treatment of disease of the heart and circulatory system
CN101084303A (zh) 2004-11-17 2007-12-05 神经干公司 用于治疗神经变性症状的人类神经细胞的移植
US8709797B2 (en) 2006-06-20 2014-04-29 Cook General Biotechnology Llc Systems and methods for cryopreservation of cells
WO2009008928A2 (en) 2007-04-13 2009-01-15 Stemnion, Inc. Methods for treating nervous system injury and disease
EP2028268A1 (en) * 2007-08-20 2009-02-25 Université Libre De Bruxelles Generation of neuronal cells from pluripotent stem cells
KR100941036B1 (ko) 2007-10-05 2010-02-05 고려대학교 산학협력단 인간 배아줄기세포로부터 척수신경계 희소돌기 아교세포생산을 위한 삼단계 분화기법
EP2217252A2 (en) 2007-10-29 2010-08-18 Hadasit Medical Research Services & Development Limited Human stem cell-derived neural precursors for treatment of autoimmune diseases of the central nervous system
US8227247B2 (en) 2007-12-20 2012-07-24 Wisconsin Alumni Research Foundation Method of generating myelinating oligodendrocytes
CA2714010C (en) 2008-01-30 2020-06-16 Corning Incorporated Synthetic surfaces for culturing stem cell derived oligodendrocyte progenitor cells
KR20110011658A (ko) 2008-05-08 2011-02-08 유니버시티 오브 로체스터 최적화된 세포 제제에 의한 수초 질환의 치료
CN102083964B (zh) 2008-05-09 2013-06-12 格拉斯哥大学大学行政评议会 与基于细胞的疗法相关的材料和方法
US20100158878A1 (en) 2008-12-23 2010-06-24 Alexandra Capela Target populations of oligodendrocyte precursor cells and methods of making and using same
CN102803472B (zh) 2009-06-25 2014-04-23 吉隆公司 去除了外来表型的由多能干细胞分化的子代细胞
WO2011059920A2 (en) 2009-11-10 2011-05-19 The J. David Gladstone Institutes Methods of generating neural stem cells
CN102160546B (zh) 2010-02-24 2016-01-20 库克通用生物科技有限责任公司 冷冻保存细胞的系统和方法
WO2012021818A2 (en) 2010-08-13 2012-02-16 Georgetown University Ggf2 and methods of use
CN103429734A (zh) 2010-10-26 2013-12-04 卡斯西部储备大学 用于产生神经胶质细胞的群体的分化方法
CN103384680B (zh) 2010-12-17 2015-08-26 拜奥拉米那公司 细胞培养基
TWI586805B (zh) 2011-01-12 2017-06-11 城戶常雄 獲得及維持在體外易於分化為寡樹突細胞－族系細胞之哺乳類神經幹細胞及／或神經祖源細胞的純或增富族群之培養方法
ES2666503T3 (es) 2012-05-16 2018-05-04 Becton, Dickinson And Company Características distintivas de superficie celular para aislar neuronas de cultivos celulares derivados de células madre pluripotentes
EP2954046B1 (en) 2013-02-06 2025-10-15 University Of Rochester Induced pluripotent cell-derived oligodendrocyte progenitor cells for the treatment of myelin disorders
US10160950B2 (en) 2013-03-01 2018-12-25 Wisconsin Alumni Research Foundation Methods of maintaining, expanding and differentiating neuronal subtype specific progenitors
KR20230058556A (ko) 2013-03-15 2023-05-03 아스텔라스 인스티튜트 포 리제너러티브 메디슨 만능 줄기 세포로부터 제조된 광수용기 및 광수용기 전구체
WO2015088625A2 (en) 2013-09-25 2015-06-18 Case Western Reserve University Compounds and methods of promoting myelination
WO2015143342A1 (en) 2014-03-21 2015-09-24 Cellular Dynamics International, Inc. Production of midbrain dopaminergic neurons and methods for the use thereof
KR102585909B1 (ko) 2014-05-22 2023-10-05 뉴욕 스템 셀 파운데이션, 인코포레이티드 다능성 줄기 세포로부터 유래된 기능성 희소돌기아교세포 및 이의 제조 및 사용 방법
WO2016063985A1 (ja) * 2014-10-24 2016-04-28 大日本住友製薬株式会社 神経組織の製造方法
CN105624116B (zh) * 2014-11-07 2020-04-07 中国科学院上海生命科学研究院 利用多能干细胞制备神经前体细胞的方法
WO2016103269A1 (en) 2014-12-23 2016-06-30 Ramot At Tel-Aviv University Ltd. Populations of neural progenitor cells and methods of producing and using same
US10286009B2 (en) 2015-05-16 2019-05-14 Asterias Biotherapeutics, Inc. Pluripotent stem cell-derived oligodendrocyte progenitor cells for the treatment of spinal cord injury
AU2017211858B2 (en) * 2016-01-27 2023-05-18 Memorial Sloan-Kettering Cancer Center Differentiation of cortical neurons from human pluripotent stem cells
CN116121189A (zh) 2016-03-30 2023-05-16 阿斯特利亚斯生物治疗股份公司 少突胶质细胞祖细胞组合物
IL281628B2 (en) 2018-09-19 2025-06-01 Lineage Cell Therapeutics Inc Methods for differentiating pluripotent stem cells in dynamic suspension culture
WO2020154533A1 (en) 2019-01-23 2020-07-30 Asterias Biotherapeutics, Inc. Dorsally-derived oligodendrocyte progenitor cells from human pluripotent stem cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004007665A2 (en) * 2002-07-16 2004-01-22 Trustees Of Columbia University In The City Of Newyork Methods for inducing differentiation of embryonic stem cells and uses thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells;Meixiang Zhang et al;《stem cell research &therapy》;20180315;第9卷(第67期);1-13 *

Also Published As

Publication number Publication date
US11603518B2 (en) 2023-03-14
CN120060141A (zh) 2025-05-30
AU2020210757B2 (en) 2026-04-16
JP2024096785A (ja) 2024-07-17
JP7469317B2 (ja) 2024-04-16
US20200231932A1 (en) 2020-07-23
WO2020154533A1 (en) 2020-07-30
KR20210121112A (ko) 2021-10-07
CA3127213A1 (en) 2020-07-30
EP3914264A1 (en) 2021-12-01
EP3914264A4 (en) 2022-11-23
CN113474451A (zh) 2021-10-01
KR102910994B1 (ko) 2026-01-12
JP2022520722A (ja) 2022-04-01
KR20260020476A (ko) 2026-02-11
AU2020210757A1 (en) 2021-08-05
US20230139899A1 (en) 2023-05-04
SG11202107732XA (en) 2021-08-30

Similar Documents

Publication Publication Date Title
CN113474451B (zh) 来自人多能干细胞的背源性少突胶质祖细胞
CN113646422B (zh) 用于在动态悬浮培养中分化多能干细胞的方法
JP2008099662A (ja) 幹細胞の培養方法
CA3182115A1 (en) Methods for differentiating stem cells into dopaminergic progenitor cells
CA3193659A1 (en) Methods for generating neural progenitor cells with a spinal cord identity
HK40124763A (zh) 来自人多能干细胞的背源性少突胶质祖细胞
HK40061262B (zh) 来自人多能干细胞的背源性少突胶质祖细胞
HK40061262A (zh) 来自人多能干细胞的背源性少突胶质祖细胞
HK40125770A (zh) 用於在动态悬浮培养中分化多能干细胞的方法
CN117242174A (zh) 生成少突胶质细胞祖细胞的方法及其用途

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40061262

Country of ref document: HK

GR01 Patent grant
GR01 Patent grant