JP7452792B2 - 気管支断端瘻治療用細胞構造体とその製造方法 - Google Patents
気管支断端瘻治療用細胞構造体とその製造方法 Download PDFInfo
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Description
ラット (F344, 3週齢)から線維芽細胞 (Fibroblast)、骨髄由来間葉系幹細胞(MSC-BM)を単離した。また、正常ラット肺毛細血管内皮細胞 (RLMVEC, Lot number:RLMV-3, Cat. No.: KBKJB210, Vec Technologies社)の細胞株を購入して使用した。それぞれの細胞を37℃、5%CO2インキュベーター内にて培養 (D-MEM / Ham's F-12 with L-Glutamine and Phenol Red, 富士フィルム和光純薬株式会社・間葉系幹細胞増殖培地2 , D12132, タカラバイオ株式会社・EGM-2, CC-3162, ロンザジャパン株式会社)した後に、サイフューズ社製3D bio printer (Regenova)を用いて、スフェロイドを融合させて固形化させ、表1(1)~(3)に示した細胞混合比の細胞構造体を作製し、上記インキュベーター内で2週間の培養期間をおいて1mm x 8mm程度の円柱状の細胞構造体を完成させた。この細胞構造体は、鑷子で把持可能な強度である。
(瘻孔閉鎖の検討方法)
実験モデルとして、上記の実施例1で作製した細胞構造体を別個体の同系統ラット (F344, 8-12週齢)の気管支に挿入し、病理・組織学的に評価等を行った。具体的には、全身麻酔下に左開胸して左気管支を露出させた。中枢側は結紮し、末梢側気管支より気管支内をブラシで擦過したのちに、培養した細胞構造体を挿入し、末梢側も結紮した。これを細胞構造体挿入群とし、コントロール群では細胞構造体を挿入しない。短期・長期の観察期間をおいた後に、犠牲死させ、病理像にて気管支内の線維化や気管支閉鎖の有無の程度を評価した。
結果を図1-3に示す。コントロール群では、図1のように気管支内腔は開存したままであった。
Claims (2)
- 気管支断端に供給されて瘻孔を閉鎖するための気管支断端瘻治療用細胞構造体の製造方法であって、間葉系幹細胞を含む複数のスフェロイドを融合させて固形化する工程を含むことを特徴とする気管支断端瘻治療用細胞構造体の製造方法。
- 前記スフェロイドは、線維芽細胞または血管内皮細胞のうちの少なくともいずれかを含むことを特徴とする請求項1の気管支断端瘻治療用細胞構造体の製造方法。
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008123614A1 (ja) | 2007-03-30 | 2008-10-16 | Kyushu University, National University Corporation | 細胞の立体構造体の製造方法 |
US20140330309A1 (en) | 2012-01-17 | 2014-11-06 | Spiration, Inc. | Systems and methods for treating fistulas in the lung and trachea |
JP2017131198A (ja) | 2016-01-29 | 2017-08-03 | 国立研究開発法人国立循環器病研究センター | 軟骨細胞塊及び移植材料の製造方法、軟骨細胞塊及び移植材料並びに複合材 |
WO2018175624A1 (en) | 2017-03-21 | 2018-09-27 | Mayo Foundation For Medical Education And Research | Methods and materials for treating fistulas |
WO2019018737A1 (en) | 2017-07-21 | 2019-01-24 | President And Fellows Of Harvard College | METHODS FOR PRODUCING MULTILAYER TUBULAR TISSUE CONSTRUCTIONS |
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Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008123614A1 (ja) | 2007-03-30 | 2008-10-16 | Kyushu University, National University Corporation | 細胞の立体構造体の製造方法 |
US20140330309A1 (en) | 2012-01-17 | 2014-11-06 | Spiration, Inc. | Systems and methods for treating fistulas in the lung and trachea |
JP2015510409A (ja) | 2012-01-17 | 2015-04-09 | スパイレーション インコーポレイテッド | 肺及び気管内の瘻を処置するシステム及び方法 |
JP2017131198A (ja) | 2016-01-29 | 2017-08-03 | 国立研究開発法人国立循環器病研究センター | 軟骨細胞塊及び移植材料の製造方法、軟骨細胞塊及び移植材料並びに複合材 |
WO2018175624A1 (en) | 2017-03-21 | 2018-09-27 | Mayo Foundation For Medical Education And Research | Methods and materials for treating fistulas |
WO2019018737A1 (en) | 2017-07-21 | 2019-01-24 | President And Fellows Of Harvard College | METHODS FOR PRODUCING MULTILAYER TUBULAR TISSUE CONSTRUCTIONS |
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