JP7432622B2 - Ｄｎａ検出方法およびｄｎａ検出システム - Google Patents

Ｄｎａ検出方法およびｄｎａ検出システム Download PDF

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JP7432622B2
JP7432622B2 JP2021570553A JP2021570553A JP7432622B2 JP 7432622 B2 JP7432622 B2 JP 7432622B2 JP 2021570553 A JP2021570553 A JP 2021570553A JP 2021570553 A JP2021570553 A JP 2021570553A JP 7432622 B2 JP7432622 B2 JP 7432622B2
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dna
gene
melting temperature
compartments
pseudogene
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JPWO2021144903A5 (https=
JPWO2021144903A1 (https=
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淳子 田中
樹生 中川
亜希子 白鳥
千宗 植松
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Hitachi High Tech Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2021570553A 2020-01-16 2020-01-16 Ｄｎａ検出方法およびｄｎａ検出システム Active JP7432622B2 (ja)

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PCT/JP2020/001171 WO2021144903A1 (ja) 2020-01-16 2020-01-16 Ｄｎａ検出方法およびｄｎａ検出システム

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JPWO2021144903A1 JPWO2021144903A1 (https=) 2021-07-22
JPWO2021144903A5 JPWO2021144903A5 (https=) 2022-08-17
JP7432622B2 true JP7432622B2 (ja) 2024-02-16

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US (1) US20230053441A1 (https=)
EP (1) EP4092134A4 (https=)
JP (1) JP7432622B2 (https=)
CN (1) CN114929894B (https=)
WO (1) WO2021144903A1 (https=)

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Publication number Priority date Publication date Assignee Title
JP2025034360A (ja) * 2023-08-31 2025-03-13 株式会社日立製作所 Ｄｎａ検出方法およびｄｎａ検出キット

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016519929A (ja) 2013-05-08 2016-07-11 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 次世代システムを用いるｈｌａ遺伝子アンプリコンのディープシーケンシングにより混合物を定量解析することによる固形臓器移植片拒絶の非侵襲的早期検出
JP2019213513A (ja) 2018-06-14 2019-12-19 株式会社日立製作所 デジタルｐｃｒの測定方法および測定装置

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JP5017552B2 (ja) * 2006-08-24 2012-09-05 社会医療法人大雄会 遺伝子検査方法及び遺伝子検査方法に使用されるキット
CN101646786A (zh) * 2007-12-26 2010-02-10 爱科来株式会社 目标核酸序列的扩增方法和用于该方法的探针
CA2789425C (en) * 2010-02-12 2020-04-28 Raindance Technologies, Inc. Digital analyte analysis with polymerase error correction
US9840737B2 (en) * 2010-06-02 2017-12-12 Canon U.S. Life Sciences, Inc. Methods and systems for sequential determination of genetic mutations and/or variants
WO2012095378A1 (en) * 2011-01-11 2012-07-19 Roche Diagnostics Gmbh High resolution melting analysis as a prescreening tool
EP2878374B1 (en) * 2013-11-29 2021-05-12 IMEC vzw Method for performing digital PCR
JP2019058071A (ja) * 2016-02-03 2019-04-18 ジェノダイブファーマ株式会社 Ｐｃｒを用いないキャプチャー法によるｈｌａ遺伝子タイピング用プローブセット及びそれを用いたタイピング方法
WO2018119443A1 (en) * 2016-12-23 2018-06-28 The Regents Of The University Of California Method and device for digital high resolution melt
JP6754301B2 (ja) * 2017-01-05 2020-09-09 株式会社日立製作所 ドロップレットデジタルｐｃｒの測定方法および測定装置
CN107099609A (zh) * 2017-06-19 2017-08-29 东莞市妇幼保健院 一种微滴式数字pcr检测低比例痕量目标基因的遗传分析方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016519929A (ja) 2013-05-08 2016-07-11 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 次世代システムを用いるｈｌａ遺伝子アンプリコンのディープシーケンシングにより混合物を定量解析することによる固形臓器移植片拒絶の非侵襲的早期検出
JP2019213513A (ja) 2018-06-14 2019-12-19 株式会社日立製作所 デジタルｐｃｒの測定方法および測定装置

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LEBLANC, M. A. et al.，Hum. Genet.，2013年，Vol. 132，pp. 1223-1234

Also Published As

Publication number Publication date
EP4092134A4 (en) 2023-10-04
EP4092134A1 (en) 2022-11-23
CN114929894A (zh) 2022-08-19
JPWO2021144903A1 (https=) 2021-07-22
US20230053441A1 (en) 2023-02-23
CN114929894B (zh) 2025-11-11
WO2021144903A1 (ja) 2021-07-22

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