CN114929894B - Dna检测方法以及dna检测系统 - Google Patents

Dna检测方法以及dna检测系统

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Publication number
CN114929894B
CN114929894B CN202080091619.1A CN202080091619A CN114929894B CN 114929894 B CN114929894 B CN 114929894B CN 202080091619 A CN202080091619 A CN 202080091619A CN 114929894 B CN114929894 B CN 114929894B
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CN
China
Prior art keywords
dna
gene
partitions
melting temperature
temperature
Prior art date
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Application number
CN202080091619.1A
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English (en)
Chinese (zh)
Other versions
CN114929894A (zh
Inventor
田中淳子
中川树生
白鸟亚希子
植松千宗
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Hitachi High Tech Corp
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Hitachi High Technologies Corp
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Publication date
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Publication of CN114929894A publication Critical patent/CN114929894A/zh
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Publication of CN114929894B publication Critical patent/CN114929894B/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202080091619.1A 2020-01-16 2020-01-16 Dna检测方法以及dna检测系统 Active CN114929894B (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2020/001171 WO2021144903A1 (ja) 2020-01-16 2020-01-16 Ｄｎａ検出方法およびｄｎａ検出システム

Publications (2)

Publication Number Publication Date
CN114929894A CN114929894A (zh) 2022-08-19
CN114929894B true CN114929894B (zh) 2025-11-11

Family

ID=76864572

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080091619.1A Active CN114929894B (zh) 2020-01-16 2020-01-16 Dna检测方法以及dna检测系统

Country Status (5)

Country Link
US (1) US20230053441A1 (https=)
EP (1) EP4092134A4 (https=)
JP (1) JP7432622B2 (https=)
CN (1) CN114929894B (https=)
WO (1) WO2021144903A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2025034360A (ja) * 2023-08-31 2025-03-13 株式会社日立製作所 Ｄｎａ検出方法およびｄｎａ検出キット

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JP5017552B2 (ja) * 2006-08-24 2012-09-05 社会医療法人大雄会 遺伝子検査方法及び遺伝子検査方法に使用されるキット
CN101646786A (zh) * 2007-12-26 2010-02-10 爱科来株式会社 目标核酸序列的扩增方法和用于该方法的探针
CA2789425C (en) * 2010-02-12 2020-04-28 Raindance Technologies, Inc. Digital analyte analysis with polymerase error correction
US9840737B2 (en) * 2010-06-02 2017-12-12 Canon U.S. Life Sciences, Inc. Methods and systems for sequential determination of genetic mutations and/or variants
WO2012095378A1 (en) * 2011-01-11 2012-07-19 Roche Diagnostics Gmbh High resolution melting analysis as a prescreening tool
WO2014180905A1 (en) * 2013-05-08 2014-11-13 Roche Diagnostics Gmbh Non-invasive early detection of solid organ transplant rejection by quantitative analysis of mixtures by deep sequencing of hla gene amplicons using next generation systems
EP2878374B1 (en) * 2013-11-29 2021-05-12 IMEC vzw Method for performing digital PCR
JP2019058071A (ja) * 2016-02-03 2019-04-18 ジェノダイブファーマ株式会社 Ｐｃｒを用いないキャプチャー法によるｈｌａ遺伝子タイピング用プローブセット及びそれを用いたタイピング方法
WO2018119443A1 (en) * 2016-12-23 2018-06-28 The Regents Of The University Of California Method and device for digital high resolution melt
JP6754301B2 (ja) * 2017-01-05 2020-09-09 株式会社日立製作所 ドロップレットデジタルｐｃｒの測定方法および測定装置
CN107099609A (zh) * 2017-06-19 2017-08-29 东莞市妇幼保健院 一种微滴式数字pcr检测低比例痕量目标基因的遗传分析方法
JP7066540B2 (ja) * 2018-06-14 2022-05-13 株式会社日立製作所 デジタルｐｃｒの測定方法および測定装置

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Cost-effective and fast KIR gene-content genotyping by multiplex melting curve analysis;Amorim Leonardo M等;《HLA》;20190325;第92卷(第6期);384-391 *
Jasmin Teresa Ney 等.High-Resolution Melting Analysis as a Sensitive Prescreening Diagnostic Tool to Detect KRAS,BRAF, PIK3CA,and AKT1 Mutations in Formalin-Fixed,Paraffin-Embedded Tissues.《ARCHIVES OF PATHOLOGY&LABORATORY MEDICINE》.2012,第136卷(第9期), 983-992. *
P063Killer-cell immunoglobulin-like receptors genotypes &haplotypes among kuwaiti families;AMEEN REEM等;《HUMAN IMMUNOLOGY》;20180831;第79卷;107 *

Also Published As

Publication number Publication date
EP4092134A4 (en) 2023-10-04
JP7432622B2 (ja) 2024-02-16
EP4092134A1 (en) 2022-11-23
CN114929894A (zh) 2022-08-19
JPWO2021144903A1 (https=) 2021-07-22
US20230053441A1 (en) 2023-02-23
WO2021144903A1 (ja) 2021-07-22

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