JP7386999B2 - 生体細胞によってコロニー形成される生体適合性ポリマーからなる３ｄスキャフォールド、及びその製造 - Google Patents

生体細胞によってコロニー形成される生体適合性ポリマーからなる３ｄスキャフォールド、及びその製造 Download PDF

Info

Publication number
JP7386999B2
JP7386999B2 JP2022530944A JP2022530944A JP7386999B2 JP 7386999 B2 JP7386999 B2 JP 7386999B2 JP 2022530944 A JP2022530944 A JP 2022530944A JP 2022530944 A JP2022530944 A JP 2022530944A JP 7386999 B2 JP7386999 B2 JP 7386999B2
Authority
JP
Japan
Prior art keywords
scaffold
photopolymerizable
cells
photocrosslinkable
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2022530944A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022548423A5 (https=
JP2022548423A (ja
Inventor
クローケ ルッツ
トーマス アレキサンダー
ラム トビアス
Original Assignee
セルブリックス ゲーエムベーハー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by セルブリックス ゲーエムベーハー filed Critical セルブリックス ゲーエムベーハー
Publication of JP2022548423A publication Critical patent/JP2022548423A/ja
Publication of JP2022548423A5 publication Critical patent/JP2022548423A5/ja
Application granted granted Critical
Publication of JP7386999B2 publication Critical patent/JP7386999B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
    • B29C64/10—Processes of additive manufacturing
    • B29C64/106—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material
    • B29C64/124—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
    • B29C64/10—Processes of additive manufacturing
    • B29C64/106—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material
    • B29C64/124—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified
    • B29C64/129—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified characterised by the energy source therefor, e.g. by global irradiation combined with a mask
    • B29C64/135—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified characterised by the energy source therefor, e.g. by global irradiation combined with a mask the energy source being concentrated, e.g. scanning lasers or focused light sources
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y10/00—Processes of additive manufacturing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y70/00—Materials specially adapted for additive manufacturing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y80/00—Products made by additive manufacturing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81C—PROCESSES OR APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OR TREATMENT OF MICROSTRUCTURAL DEVICES OR SYSTEMS
    • B81C1/00—Manufacture or treatment of devices or systems in or on a substrate
    • B81C1/00015—Manufacture or treatment of devices or systems in or on a substrate for manufacturing microsystems
    • B81C1/00023—Manufacture or treatment of devices or systems in or on a substrate for manufacturing microsystems without movable or flexible elements
    • B81C1/00119—Arrangement of basic structures like cavities or channels, e.g. suitable for microfluidic systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81B—MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
    • B81B2201/00—Specific applications of microelectromechanical systems
    • B81B2201/05—Microfluidics
    • B81B2201/051—Micromixers, microreactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81B—MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
    • B81B2201/00—Specific applications of microelectromechanical systems
    • B81B2201/05—Microfluidics
    • B81B2201/058—Microfluidics not provided for in B81B2201/051 - B81B2201/054
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81B—MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
    • B81B2203/00—Basic microelectromechanical structures
    • B81B2203/03—Static structures
    • B81B2203/0315—Cavities
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2537/00—Supports and/or coatings for cell culture characterised by physical or chemical treatment
    • C12N2537/10—Cross-linking

Landscapes

  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Manufacturing & Machinery (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Materials Engineering (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Optics & Photonics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Mechanical Engineering (AREA)
  • Molecular Biology (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Materials For Medical Uses (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
JP2022530944A 2019-11-27 2020-11-20 生体細胞によってコロニー形成される生体適合性ポリマーからなる３ｄスキャフォールド、及びその製造 Active JP7386999B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102019132214.6 2019-11-27
DE102019132214.6A DE102019132214B3 (de) 2019-11-27 2019-11-27 Mit biologischen Zellen besiedeltes 3D-Gerüst aus biokompatiblem Polymer und dessen Herstellung
PCT/DE2020/100986 WO2021104571A1 (de) 2019-11-27 2020-11-20 Mit biologischen zellen besiedeltes 3d-gerüst aus biokompatiblem polymer und dessen herstellung

Publications (3)

Publication Number Publication Date
JP2022548423A JP2022548423A (ja) 2022-11-18
JP2022548423A5 JP2022548423A5 (https=) 2023-02-27
JP7386999B2 true JP7386999B2 (ja) 2023-11-27

Family

ID=73740157

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022530944A Active JP7386999B2 (ja) 2019-11-27 2020-11-20 生体細胞によってコロニー形成される生体適合性ポリマーからなる３ｄスキャフォールド、及びその製造

Country Status (7)

Country Link
US (1) US11993767B2 (https=)
EP (1) EP4065371A1 (https=)
JP (1) JP7386999B2 (https=)
CN (1) CN115943076A (https=)
DE (1) DE102019132214B3 (https=)
IL (1) IL293339A (https=)
WO (1) WO2021104571A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102022132519A1 (de) * 2022-12-07 2024-06-13 Cellbricks Gmbh Durch lichtbasierte strukturierende Aushärtung hergestelltes 3D-Fettgewebegerüst zur Verwendung als Brustimplantat
CN117565386B (zh) * 2024-01-17 2024-03-22 中国科学院化学研究所 一种细胞或类器官芯片及其制备方法与应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030175410A1 (en) 2002-03-18 2003-09-18 Campbell Phil G. Method and apparatus for preparing biomimetic scaffold
WO2009042671A1 (en) 2007-09-24 2009-04-02 The Board Of Trustees Of The University Of Illinois Three-dimensional microfabricated bioreactors with embedded capillary network
JP2017536113A (ja) 2014-11-10 2017-12-07 テヒーニィシエ ウニヴェルジテート ベルリン ３次元多細胞物体を作成するための方法と装置

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005095581A1 (en) * 2004-03-19 2005-10-13 Macropore Biosurgery Inc. Cell carrier and cell carrier containment devices containing regenerative cells
DE102007020302B4 (de) 2007-04-20 2012-03-22 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verbesserte dreidimensionale biokompatible Gerüststruktur, die Nanopartikel beinhaltet
EP2203548A4 (en) 2007-10-11 2014-01-15 Agency Science Tech & Res FORMATION OF A CELL STRUCTURE WITH A TRANSIENT LINKER IN A CAGE
US8592574B2 (en) 2009-01-22 2013-11-26 Quegen Biotech Co., Ltd. Beta-glucan-based scaffold for biological tissue engineering using radiation fusion technology, and production method therefor
US8691974B2 (en) * 2009-09-28 2014-04-08 Virginia Tech Intellectual Properties, Inc. Three-dimensional bioprinting of biosynthetic cellulose (BC) implants and scaffolds for tissue engineering
SG11201806445UA (en) 2016-01-29 2018-08-30 Nat Cerebral & Cardiovascular Ct Cell mass, cell structure, and three-dimensional tissue body
CN107058098B (zh) 2017-03-01 2025-08-15 北京科技大学 一种基于3d打印技术的细胞图案化培养的装置及方法
DE102018204624A1 (de) 2018-03-27 2019-10-02 Robert Bosch Gmbh Verfahren und mikrofluidische Vorrichtung zur Aliquotierung einer Probenflüssigkeit unter Verwendung einer Versiegelungsflüssigkeit, Verfahren zum Herstellen einer mikrofluidischen Vorrichtung und mikrofluidisches System
DE102018111751A1 (de) * 2018-05-16 2019-11-21 Cellbricks Gmbh Verfahren zur Herstellung eines Zellkultureinsatzes mit mindestens einer Membran

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030175410A1 (en) 2002-03-18 2003-09-18 Campbell Phil G. Method and apparatus for preparing biomimetic scaffold
WO2009042671A1 (en) 2007-09-24 2009-04-02 The Board Of Trustees Of The University Of Illinois Three-dimensional microfabricated bioreactors with embedded capillary network
JP2017536113A (ja) 2014-11-10 2017-12-07 テヒーニィシエ ウニヴェルジテート ベルリン ３次元多細胞物体を作成するための方法と装置

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Kuo, A. P. et al.，"High-Precision Stereolithography of Biomicrofluidic Devices"，Adv. Mater. Technol.，2019年01月03日，Vol. 4; 1800395，pp. 1-11
Xia, C. et al.，"3D microfabricated bioreactor with capillaries"，Biomed. Microdevices，2009年，Vol. 11，pp. 1309-1315

Also Published As

Publication number Publication date
CN115943076A (zh) 2023-04-07
WO2021104571A1 (de) 2021-06-03
DE102019132214B3 (de) 2021-04-29
JP2022548423A (ja) 2022-11-18
IL293339A (en) 2022-07-01
EP4065371A1 (de) 2022-10-05
US11993767B2 (en) 2024-05-28
US20230133963A1 (en) 2023-05-04

Similar Documents

Publication Publication Date Title
AU2015345206B2 (en) Method and device for producing a three-dimensional, multi-cell object
US12201982B2 (en) 3D scaffold comprising a biocompatible polymer with a colonization chamber open at the top for biological cells, and with a canal vessel surrounding the colonization chamber
US12584103B2 (en) Hypothermic 3D bioprinting of living tissues supported by perfusable vasculature
Biju et al. Role of three-dimensional cell culture in therapeutics and diagnostics: an updated review
Taebnia et al. Dual-material 3D-printed intestinal model devices with integrated villi-like scaffolds
JP7386999B2 (ja) 生体細胞によってコロニー形成される生体適合性ポリマーからなる３ｄスキャフォールド、及びその製造
US20210054324A1 (en) 3d spatially patterned lymph node on a microfluidic device
Ge et al. Development of multi-dimensional cell co-culture via a novel microfluidic chip fabricated by DMD-based optical projection lithography
CN113652389B (zh) 一种用于药物筛选的三维水凝胶阵列的高通量制备方法
US20250283020A1 (en) Microphysiological system and uses thereof
EP3746541B1 (en) Devices and methods for three-dimensional growth of cells
DE102019132180B3 (de) 3D-Gerüst aus biokompatiblem Polymer mit überdachtem Hohlraum zur Besiedlung mit biologischen Zellen
Simińska-Stanny et al. Controlling Flow Dynamics and Permeability in Perfusable Vascular Constructs Using Volumetric 3D Printing
Naderi Rapidly prototyping complex microfluidics through stereolithography
Zeußel On the way to bioinspired scaffolds: a study on light assisted additive manufacturing, materials and functionalization
Kruse Tissue Engineering Using 3D Template Casting
Yamahira et al. Masato Tamura1, Toshiyuki Takagi1, Eri Nakatani1, 2, Yuta Kusama1, 2, Kimio Sumaru1, Shinji Sugiura1 and Toshiyuki Kanamori1
Heidari Fabrication of Extremely Soft Cellular Hydrogels for Vascular Modelling
IT202100025460A1 (it) Dispositivo lab-on-chip per studiare la migrazione cellulare in sistemi tridimensionali e relativo metodo di utilizzo
Nguyen Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues
HK1236016A1 (en) Method and device for producing a three-dimensional, multi-cell object

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20220726

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20220726

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20221115

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230213

A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20230213

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20230412

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20230704

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20230911

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20231030

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20231114

R150 Certificate of patent or registration of utility model

Ref document number: 7386999

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150