CN115943076A - 由生物相容性聚合物构成的定植有生物细胞的3d支架及其制造 - Google Patents

由生物相容性聚合物构成的定植有生物细胞的3d支架及其制造 Download PDF

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Publication number
CN115943076A
CN115943076A CN202080082748.4A CN202080082748A CN115943076A CN 115943076 A CN115943076 A CN 115943076A CN 202080082748 A CN202080082748 A CN 202080082748A CN 115943076 A CN115943076 A CN 115943076A
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scaffold
liquid
photopolymerizable
biological cells
photocrosslinkable
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Chinese (zh)
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亚历山大·托马斯
托比亚斯·拉姆
路茨·克洛克
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Cell Building Blocks Ltd
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Cell Building Blocks Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
    • B29C64/10—Processes of additive manufacturing
    • B29C64/106—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material
    • B29C64/124—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
    • B29C64/10—Processes of additive manufacturing
    • B29C64/106—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material
    • B29C64/124—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified
    • B29C64/129—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified characterised by the energy source therefor, e.g. by global irradiation combined with a mask
    • B29C64/135—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified characterised by the energy source therefor, e.g. by global irradiation combined with a mask the energy source being concentrated, e.g. scanning lasers or focused light sources
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y10/00—Processes of additive manufacturing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y70/00—Materials specially adapted for additive manufacturing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y80/00—Products made by additive manufacturing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81C—PROCESSES OR APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OR TREATMENT OF MICROSTRUCTURAL DEVICES OR SYSTEMS
    • B81C1/00—Manufacture or treatment of devices or systems in or on a substrate
    • B81C1/00015—Manufacture or treatment of devices or systems in or on a substrate for manufacturing microsystems
    • B81C1/00023—Manufacture or treatment of devices or systems in or on a substrate for manufacturing microsystems without movable or flexible elements
    • B81C1/00119—Arrangement of basic structures like cavities or channels, e.g. suitable for microfluidic systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81B—MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
    • B81B2201/00—Specific applications of microelectromechanical systems
    • B81B2201/05—Microfluidics
    • B81B2201/051—Micromixers, microreactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81B—MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
    • B81B2201/00—Specific applications of microelectromechanical systems
    • B81B2201/05—Microfluidics
    • B81B2201/058—Microfluidics not provided for in B81B2201/051 - B81B2201/054
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81B—MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
    • B81B2203/00—Basic microelectromechanical structures
    • B81B2203/03—Static structures
    • B81B2203/0315—Cavities
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2537/00—Supports and/or coatings for cell culture characterised by physical or chemical treatment
    • C12N2537/10—Cross-linking

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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Manufacturing & Machinery (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Materials Engineering (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Optics & Photonics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Mechanical Engineering (AREA)
  • Molecular Biology (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Materials For Medical Uses (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
CN202080082748.4A 2019-11-27 2020-11-20 由生物相容性聚合物构成的定植有生物细胞的3d支架及其制造 Pending CN115943076A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102019132214.6 2019-11-27
DE102019132214.6A DE102019132214B3 (de) 2019-11-27 2019-11-27 Mit biologischen Zellen besiedeltes 3D-Gerüst aus biokompatiblem Polymer und dessen Herstellung
PCT/DE2020/100986 WO2021104571A1 (de) 2019-11-27 2020-11-20 Mit biologischen zellen besiedeltes 3d-gerüst aus biokompatiblem polymer und dessen herstellung

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Publication Number Publication Date
CN115943076A true CN115943076A (zh) 2023-04-07

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CN202080082748.4A Pending CN115943076A (zh) 2019-11-27 2020-11-20 由生物相容性聚合物构成的定植有生物细胞的3d支架及其制造

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US (1) US11993767B2 (https=)
EP (1) EP4065371A1 (https=)
JP (1) JP7386999B2 (https=)
CN (1) CN115943076A (https=)
DE (1) DE102019132214B3 (https=)
IL (1) IL293339A (https=)
WO (1) WO2021104571A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102022132519A1 (de) * 2022-12-07 2024-06-13 Cellbricks Gmbh Durch lichtbasierte strukturierende Aushärtung hergestelltes 3D-Fettgewebegerüst zur Verwendung als Brustimplantat
CN117565386B (zh) * 2024-01-17 2024-03-22 中国科学院化学研究所 一种细胞或类器官芯片及其制备方法与应用

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005095581A1 (en) * 2004-03-19 2005-10-13 Macropore Biosurgery Inc. Cell carrier and cell carrier containment devices containing regenerative cells
WO2009048435A1 (en) * 2007-10-11 2009-04-16 Agency For Science, Technology And Research Forming cell structure with transient linker in cage
CN101679948A (zh) * 2007-04-20 2010-03-24 弗劳恩霍芬-格泽尔沙夫特祖尔弗德伦德尔安格万特福施有限公司 细胞培养系统
CN102292113A (zh) * 2009-01-22 2011-12-21 株式会社奎真生物技术 通过辐射融合技术制造的用于生物组织工程的β-葡聚糖基支架及其制造方法
CN107058098A (zh) * 2017-03-01 2017-08-18 北京科技大学 一种基于3d打印技术的细胞图案化培养的装置及方法
CN107148302A (zh) * 2014-11-10 2017-09-08 柏林工业大学 用于生产三维多细胞物体的方法和装置
US20190040359A1 (en) * 2016-01-29 2019-02-07 National Cerebral And Cardiovascular Center Cell mass, cell structure, and three-dimensional tissue body
WO2019185508A1 (de) * 2018-03-27 2019-10-03 Robert Bosch Gmbh Verfahren und mikrofluidische vorrichtung zur aliquotierung einer probenflüssigkeit unter verwendung einer versiegelungsflüssigkeit, verfahren zum herstellen einer mikrofluidischen vorrichtung und mikrofluidisches system

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003079985A2 (en) 2002-03-18 2003-10-02 Carnegie Mellon University Method and apparatus for preparing biomimetic scaffold
WO2009042671A1 (en) * 2007-09-24 2009-04-02 The Board Of Trustees Of The University Of Illinois Three-dimensional microfabricated bioreactors with embedded capillary network
US8691974B2 (en) * 2009-09-28 2014-04-08 Virginia Tech Intellectual Properties, Inc. Three-dimensional bioprinting of biosynthetic cellulose (BC) implants and scaffolds for tissue engineering
DE102018111751A1 (de) * 2018-05-16 2019-11-21 Cellbricks Gmbh Verfahren zur Herstellung eines Zellkultureinsatzes mit mindestens einer Membran

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005095581A1 (en) * 2004-03-19 2005-10-13 Macropore Biosurgery Inc. Cell carrier and cell carrier containment devices containing regenerative cells
CN101679948A (zh) * 2007-04-20 2010-03-24 弗劳恩霍芬-格泽尔沙夫特祖尔弗德伦德尔安格万特福施有限公司 细胞培养系统
WO2009048435A1 (en) * 2007-10-11 2009-04-16 Agency For Science, Technology And Research Forming cell structure with transient linker in cage
CN101883842A (zh) * 2007-10-11 2010-11-10 新加坡科技研究局 在栅栏内用短期连接体形成细胞结构
CN102292113A (zh) * 2009-01-22 2011-12-21 株式会社奎真生物技术 通过辐射融合技术制造的用于生物组织工程的β-葡聚糖基支架及其制造方法
CN107148302A (zh) * 2014-11-10 2017-09-08 柏林工业大学 用于生产三维多细胞物体的方法和装置
US20190040359A1 (en) * 2016-01-29 2019-02-07 National Cerebral And Cardiovascular Center Cell mass, cell structure, and three-dimensional tissue body
CN107058098A (zh) * 2017-03-01 2017-08-18 北京科技大学 一种基于3d打印技术的细胞图案化培养的装置及方法
WO2019185508A1 (de) * 2018-03-27 2019-10-03 Robert Bosch Gmbh Verfahren und mikrofluidische vorrichtung zur aliquotierung einer probenflüssigkeit unter verwendung einer versiegelungsflüssigkeit, verfahren zum herstellen einer mikrofluidischen vorrichtung und mikrofluidisches system

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Publication number Publication date
WO2021104571A1 (de) 2021-06-03
DE102019132214B3 (de) 2021-04-29
JP2022548423A (ja) 2022-11-18
IL293339A (en) 2022-07-01
EP4065371A1 (de) 2022-10-05
JP7386999B2 (ja) 2023-11-27
US11993767B2 (en) 2024-05-28
US20230133963A1 (en) 2023-05-04

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