DE102019132214B3 - Mit biologischen Zellen besiedeltes 3D-Gerüst aus biokompatiblem Polymer und dessen Herstellung - Google Patents

Mit biologischen Zellen besiedeltes 3D-Gerüst aus biokompatiblem Polymer und dessen Herstellung Download PDF

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Publication number
DE102019132214B3
DE102019132214B3 DE102019132214.6A DE102019132214A DE102019132214B3 DE 102019132214 B3 DE102019132214 B3 DE 102019132214B3 DE 102019132214 A DE102019132214 A DE 102019132214A DE 102019132214 B3 DE102019132214 B3 DE 102019132214B3
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framework
photopolymerizable
biological cells
cells
photocrosslinkable
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DE102019132214.6A
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German (de)
English (en)
Inventor
Lutz Kloke
Alexander Thomas
Tobias Lam
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Cellbricks GmbH
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Cellbricks GmbH
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Priority to DE102019132214.6A priority Critical patent/DE102019132214B3/de
Priority to EP20820324.0A priority patent/EP4065371A1/de
Priority to CN202080082748.4A priority patent/CN115943076A/zh
Priority to PCT/DE2020/100986 priority patent/WO2021104571A1/de
Priority to IL293339A priority patent/IL293339A/en
Priority to US17/779,100 priority patent/US11993767B2/en
Priority to JP2022530944A priority patent/JP7386999B2/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
    • B29C64/10—Processes of additive manufacturing
    • B29C64/106—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material
    • B29C64/124—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
    • B29C64/10—Processes of additive manufacturing
    • B29C64/106—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material
    • B29C64/124—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified
    • B29C64/129—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified characterised by the energy source therefor, e.g. by global irradiation combined with a mask
    • B29C64/135—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified characterised by the energy source therefor, e.g. by global irradiation combined with a mask the energy source being concentrated, e.g. scanning lasers or focused light sources
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y10/00—Processes of additive manufacturing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y70/00—Materials specially adapted for additive manufacturing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y80/00—Products made by additive manufacturing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81C—PROCESSES OR APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OR TREATMENT OF MICROSTRUCTURAL DEVICES OR SYSTEMS
    • B81C1/00—Manufacture or treatment of devices or systems in or on a substrate
    • B81C1/00015—Manufacture or treatment of devices or systems in or on a substrate for manufacturing microsystems
    • B81C1/00023—Manufacture or treatment of devices or systems in or on a substrate for manufacturing microsystems without movable or flexible elements
    • B81C1/00119—Arrangement of basic structures like cavities or channels, e.g. suitable for microfluidic systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81B—MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
    • B81B2201/00—Specific applications of microelectromechanical systems
    • B81B2201/05—Microfluidics
    • B81B2201/051—Micromixers, microreactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81B—MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
    • B81B2201/00—Specific applications of microelectromechanical systems
    • B81B2201/05—Microfluidics
    • B81B2201/058—Microfluidics not provided for in B81B2201/051 - B81B2201/054
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81B—MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
    • B81B2203/00—Basic microelectromechanical structures
    • B81B2203/03—Static structures
    • B81B2203/0315—Cavities
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2537/00—Supports and/or coatings for cell culture characterised by physical or chemical treatment
    • C12N2537/10—Cross-linking

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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Manufacturing & Machinery (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Materials Engineering (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Optics & Photonics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Mechanical Engineering (AREA)
  • Molecular Biology (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Materials For Medical Uses (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
DE102019132214.6A 2019-11-27 2019-11-27 Mit biologischen Zellen besiedeltes 3D-Gerüst aus biokompatiblem Polymer und dessen Herstellung Active DE102019132214B3 (de)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DE102019132214.6A DE102019132214B3 (de) 2019-11-27 2019-11-27 Mit biologischen Zellen besiedeltes 3D-Gerüst aus biokompatiblem Polymer und dessen Herstellung
EP20820324.0A EP4065371A1 (de) 2019-11-27 2020-11-20 Mit biologischen zellen besiedeltes 3d-gerüst aus biokompatiblem polymer und dessen herstellung
CN202080082748.4A CN115943076A (zh) 2019-11-27 2020-11-20 由生物相容性聚合物构成的定植有生物细胞的3d支架及其制造
PCT/DE2020/100986 WO2021104571A1 (de) 2019-11-27 2020-11-20 Mit biologischen zellen besiedeltes 3d-gerüst aus biokompatiblem polymer und dessen herstellung
IL293339A IL293339A (en) 2019-11-27 2020-11-20 3d scaffold of a biocompatible polymer being colonised with biological cells, and production thereof
US17/779,100 US11993767B2 (en) 2019-11-27 2020-11-20 Method for producing 3D, biocompatible polymer scaffold with a cell-filled cavity
JP2022530944A JP7386999B2 (ja) 2019-11-27 2020-11-20 生体細胞によってコロニー形成される生体適合性ポリマーからなる３ｄスキャフォールド、及びその製造

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102019132214.6A DE102019132214B3 (de) 2019-11-27 2019-11-27 Mit biologischen Zellen besiedeltes 3D-Gerüst aus biokompatiblem Polymer und dessen Herstellung

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DE102019132214B3 true DE102019132214B3 (de) 2021-04-29

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US (1) US11993767B2 (https=)
EP (1) EP4065371A1 (https=)
JP (1) JP7386999B2 (https=)
CN (1) CN115943076A (https=)
DE (1) DE102019132214B3 (https=)
IL (1) IL293339A (https=)
WO (1) WO2021104571A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024120579A1 (de) * 2022-12-07 2024-06-13 Cellbricks Gmbh Durch lichtbasierte strukturierende aushärtung hergestelltes 3d-fettgewebegerüst zur verwendung als brustimplantat

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117565386B (zh) * 2024-01-17 2024-03-22 中国科学院化学研究所 一种细胞或类器官芯片及其制备方法与应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011038373A2 (en) * 2009-09-28 2011-03-31 Virginia Tech Intellectual Properties, Inc. Three-dimensional bioprinting of biosynthetic cellulose (bc) implants and scaffolds for tissue engineering
WO2019219605A1 (de) * 2018-05-16 2019-11-21 Cellbricks Gmbh Verfahren zur herstellung eines zellkultureinsatzes mit mindestens einer membran

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WO2003079985A2 (en) 2002-03-18 2003-10-02 Carnegie Mellon University Method and apparatus for preparing biomimetic scaffold
WO2005095581A1 (en) * 2004-03-19 2005-10-13 Macropore Biosurgery Inc. Cell carrier and cell carrier containment devices containing regenerative cells
DE102007020302B4 (de) 2007-04-20 2012-03-22 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verbesserte dreidimensionale biokompatible Gerüststruktur, die Nanopartikel beinhaltet
WO2009042671A1 (en) * 2007-09-24 2009-04-02 The Board Of Trustees Of The University Of Illinois Three-dimensional microfabricated bioreactors with embedded capillary network
EP2203548A4 (en) 2007-10-11 2014-01-15 Agency Science Tech & Res FORMATION OF A CELL STRUCTURE WITH A TRANSIENT LINKER IN A CAGE
US8592574B2 (en) 2009-01-22 2013-11-26 Quegen Biotech Co., Ltd. Beta-glucan-based scaffold for biological tissue engineering using radiation fusion technology, and production method therefor
PL3018531T3 (pl) 2014-11-10 2020-11-16 Technische Universität Berlin Sposób i urządzenie do wytwarzania trójwymiarowego obiektu wielokomórkowego
SG11201806445UA (en) 2016-01-29 2018-08-30 Nat Cerebral & Cardiovascular Ct Cell mass, cell structure, and three-dimensional tissue body
CN107058098B (zh) 2017-03-01 2025-08-15 北京科技大学 一种基于3d打印技术的细胞图案化培养的装置及方法
DE102018204624A1 (de) 2018-03-27 2019-10-02 Robert Bosch Gmbh Verfahren und mikrofluidische Vorrichtung zur Aliquotierung einer Probenflüssigkeit unter Verwendung einer Versiegelungsflüssigkeit, Verfahren zum Herstellen einer mikrofluidischen Vorrichtung und mikrofluidisches System

Patent Citations (2)

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WO2011038373A2 (en) * 2009-09-28 2011-03-31 Virginia Tech Intellectual Properties, Inc. Three-dimensional bioprinting of biosynthetic cellulose (bc) implants and scaffolds for tissue engineering
WO2019219605A1 (de) * 2018-05-16 2019-11-21 Cellbricks Gmbh Verfahren zur herstellung eines zellkultureinsatzes mit mindestens einer membran

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024120579A1 (de) * 2022-12-07 2024-06-13 Cellbricks Gmbh Durch lichtbasierte strukturierende aushärtung hergestelltes 3d-fettgewebegerüst zur verwendung als brustimplantat
DE102022132519A1 (de) * 2022-12-07 2024-06-13 Cellbricks Gmbh Durch lichtbasierte strukturierende Aushärtung hergestelltes 3D-Fettgewebegerüst zur Verwendung als Brustimplantat

Also Published As

Publication number Publication date
CN115943076A (zh) 2023-04-07
WO2021104571A1 (de) 2021-06-03
JP2022548423A (ja) 2022-11-18
IL293339A (en) 2022-07-01
EP4065371A1 (de) 2022-10-05
JP7386999B2 (ja) 2023-11-27
US11993767B2 (en) 2024-05-28
US20230133963A1 (en) 2023-05-04

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