JP7366754B2 - 高精度粒子選別のための電気的検出と光学的検出を組み合わせたマイクロ流体システム、およびその方法 - Google Patents

高精度粒子選別のための電気的検出と光学的検出を組み合わせたマイクロ流体システム、およびその方法 Download PDF

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JP7366754B2
JP7366754B2 JP2019563338A JP2019563338A JP7366754B2 JP 7366754 B2 JP7366754 B2 JP 7366754B2 JP 2019563338 A JP2019563338 A JP 2019563338A JP 2019563338 A JP2019563338 A JP 2019563338A JP 7366754 B2 JP7366754 B2 JP 7366754B2
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microfluidic channel
particles
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sorting
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JP2020508468A (ja
JP2020508468A5 (https=
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ナセフ，ハニー
バラクリシュナン，カーシック
ケサヴァラジュ，アナンド
トゥミネリ，ヴィンセント
アンワー，ジョージ
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ノデクサス インコーポレーテッド
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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    • G01—MEASURING; TESTING
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    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
    • B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
    • B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
    • B07C5/34—Sorting according to other particular properties
    • B07C5/342—Sorting according to other particular properties according to optical properties, e.g. colour
    • B07C5/3425—Sorting according to other particular properties according to optical properties, e.g. colour of granular material, e.g. ore particles, grain
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
    • B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
    • B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
    • B07C5/34—Sorting according to other particular properties
    • B07C5/344—Sorting according to other particular properties according to electric or electromagnetic properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0874—Three dimensional network
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
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    • B01L9/527—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for microfluidic devices, e.g. used for lab-on-a-chip
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
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    • G01N15/10—Investigating individual particles
    • G01N2015/1028—Sorting particles

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Fluid Mechanics (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
JP2019563338A 2017-02-07 2018-02-06 高精度粒子選別のための電気的検出と光学的検出を組み合わせたマイクロ流体システム、およびその方法 Active JP7366754B2 (ja)

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JP2023175614A JP7527453B2 (ja) 2017-02-07 2023-10-11 高精度粒子選別のための電気的検出と光学的検出を組み合わせたマイクロ流体システム、およびその方法
JP2024117735A JP2024167184A (ja) 2017-02-07 2024-07-23 高精度粒子選別のための電気的検出と光学的検出を組み合わせたマイクロ流体システム、およびその方法

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US201762456069P 2017-02-07 2017-02-07
US62/456,069 2017-02-07
PCT/US2018/017036 WO2018148194A1 (en) 2017-02-07 2018-02-06 Microfluidic system with combined electrical and optical detection for high accuracy particle sorting and methods thereof

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JP2020508468A JP2020508468A (ja) 2020-03-19
JP2020508468A5 JP2020508468A5 (https=) 2021-03-18
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JP2023175614A Active JP7527453B2 (ja) 2017-02-07 2023-10-11 高精度粒子選別のための電気的検出と光学的検出を組み合わせたマイクロ流体システム、およびその方法
JP2024117735A Pending JP2024167184A (ja) 2017-02-07 2024-07-23 高精度粒子選別のための電気的検出と光学的検出を組み合わせたマイクロ流体システム、およびその方法

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JP2024117735A Pending JP2024167184A (ja) 2017-02-07 2024-07-23 高精度粒子選別のための電気的検出と光学的検出を組み合わせたマイクロ流体システム、およびその方法

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EP (1) EP3579973A4 (https=)
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Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3933378A4 (en) * 2019-02-27 2022-11-30 Kyocera Corporation PARTICLE SEPARATION AND MEASURING DEVICE AND PARTICLE SEPARATION AND MEASURING DEVICE
WO2020219800A1 (en) 2019-04-24 2020-10-29 Bennubio Inc. Positionally assisted negative particle rejection (panr) to sort and enrich target cells of interest
CN114729872A (zh) * 2019-11-20 2022-07-08 索尼集团公司 微芯片、样本分选试剂盒和微粒分选装置
JP7477378B2 (ja) 2020-06-19 2024-05-01 シスメックス株式会社 粒子ソーター、粒子分取方法およびマイクロ流路カートリッジ
DK181994B1 (en) * 2021-09-28 2025-05-15 Samplix Aps Instrument for sorting of particles
EP4255632A1 (en) * 2020-12-01 2023-10-11 Samplix ApS System and method for sorting of particles
TWI785543B (zh) * 2021-03-18 2022-12-01 歐陽自坤 生物粒子檢測系統及檢測方法
GB2621756B (en) * 2021-04-27 2026-04-01 Boe Technology Group Co Ltd Microfluidic chip, box body device, and microfluidic device
US20230285970A1 (en) * 2022-03-11 2023-09-14 Hewlett-Packard Development Company, L.P. Apparatuses with fluid droplet generators coupled to reaction regions and fluid ejectors
WO2024064914A1 (en) 2022-09-22 2024-03-28 Sigil Biosciences, Inc. Devices and systems for isolating particles in solution by particle permitivity
CN115468942B (zh) * 2022-09-22 2025-09-09 深圳高性能医疗器械国家研究院有限公司 一种单分子荧光检测系统及锥形毛细微管制备方法
WO2024155709A1 (en) * 2023-01-18 2024-07-25 Nodexus Inc. Microfluidic particle sorting
CN116207194B (zh) * 2023-05-05 2023-08-01 武汉精立电子技术有限公司 一种Mini LED芯片自动化制程装置及方法
WO2025106429A1 (en) * 2023-11-15 2025-05-22 Generate Biomedicines, Inc. Systems and methods for image-based cell sorting

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003502655A (ja) 1999-06-22 2003-01-21 アジレント・テクノロジーズ・インコーポレーテッド 微小流体デバイスの操作用装置
JP2006220423A (ja) 2005-02-08 2006-08-24 Japan Science & Technology Agency ゲル電極付セルソーターチップ
JP2007533971A (ja) 2003-09-30 2007-11-22 シンギュレックス・インコーポレイテッド 粒子検出の分析精度を改善する方法
WO2016182034A1 (ja) 2015-05-12 2016-11-17 株式会社オンチップ・バイオテクノロジーズ 単一粒子解析方法およびその解析のためのシステム

Family Cites Families (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE48477T1 (de) * 1984-09-11 1989-12-15 Partec Ag Verfahren und vorrichtung zur sortierung von mikroskopischen partikeln.
JPS61160038A (ja) 1985-01-08 1986-07-19 Sumitomo Electric Ind Ltd 粒子検出器
US4778657A (en) 1985-09-10 1988-10-18 Kernforschungszentrum Karlsruhe Gmbh Apparatus for determining the characteristics of particles suspended in liquids
US5846708A (en) 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
US6849423B2 (en) * 2000-11-29 2005-02-01 Picoliter Inc Focused acoustics for detection and sorting of fluid volumes
WO2002059575A2 (en) 2001-01-23 2002-08-01 The Trustees Of Princeton University, Princeton University Method and apparatus for analysis of biological solutions
JP2002286594A (ja) * 2001-03-28 2002-10-03 Olympus Optical Co Ltd 微粒子搬送機構
CN1325658C (zh) 2001-04-23 2007-07-11 三星电子株式会社 包含mosfet分子检测芯片和采用该芯片的分子检测装置以及使用该装置的分子检测方法
AU2002318269A1 (en) * 2001-07-18 2003-03-03 The Regents Of The University Of Michigan Gas-focusing flow cytometer cell and flow cytometer detection system with waveguide optics
EP1421365A1 (en) * 2001-07-19 2004-05-26 Tufts University Optical array device and methods of use thereof for screening, analysis and manipulation of particles
US7179420B2 (en) * 2001-10-25 2007-02-20 Techelan, Llc Apparatus comprising a particle sorter/dispenser and method therefor
CA2480728A1 (en) * 2002-04-01 2003-10-16 Fluidigm Corporation Microfluidic particle-analysis systems
BR0309321A (pt) * 2002-04-17 2005-03-29 Cytonome Inc Método e aparelho para selecionar partìculas
US7389879B2 (en) * 2004-01-21 2008-06-24 Hewlett-Packard Development Company, L.P. Sorting particles
CN100582766C (zh) 2004-03-05 2010-01-20 安捷伦科技有限公司 检测通道内剖面缩小的非接触检测单元
US7392908B2 (en) 2005-01-12 2008-07-01 Beckman Coulter, Inc. Methods and apparatus for sorting particles hydraulically
EP1855114A1 (en) 2005-03-01 2007-11-14 Rohm Co., Ltd. Microchannel and microfluid chip
CA2607579A1 (en) 2005-05-06 2006-11-23 The Regents Of The University Of California Microfluidic system for identifying or sizing individual particles passing through a channel
WO2007005907A1 (en) * 2005-07-01 2007-01-11 Honeywell International, Inc. A molded cartridge with 3-d hydrodynamic focusing
US20070059683A1 (en) 2005-09-15 2007-03-15 Tom Barber Veterinary diagnostic system
US8290625B2 (en) 2007-04-04 2012-10-16 Beckman Coulter, Inc. Flow cytometer sorter
US8691164B2 (en) * 2007-04-20 2014-04-08 Celula, Inc. Cell sorting system and methods
KR100830221B1 (ko) 2007-04-23 2008-05-16 (주)에이치시티 입자 측정장치
WO2009032827A2 (en) 2007-09-04 2009-03-12 Purdue Research Foundation Electroporative flow cytometry
KR20110030415A (ko) 2008-01-22 2011-03-23 인터젠엑스 인크. 만능 샘플 제조 시스템 및 집적 분석 시스템에서의 용도
US20100099198A1 (en) 2008-07-11 2010-04-22 Board Of Regents, The University Of Texas System Apparatus and system for pattern recognition sensing for biomolecules
ATE544063T1 (de) 2008-07-17 2012-02-15 Koninkl Philips Electronics Nv Nanoporenvorrichtung und verfahren zur nukleinsäureanalyse
JP5382852B2 (ja) 2009-02-06 2014-01-08 株式会社オンチップ・バイオテクノロジーズ 使い捨てチップ型フローセルとそれを用いたフローサイトメーター
JP5487638B2 (ja) * 2009-02-17 2014-05-07 ソニー株式会社 微小粒子分取のための装置及びマイクロチップ
WO2010104993A2 (en) * 2009-03-10 2010-09-16 The Regents Of The University Of California Fluidic flow cytometry devices and particle sensing based on signal-encoding
US9364831B2 (en) 2009-08-08 2016-06-14 The Regents Of The University Of California Pulsed laser triggered high speed microfluidic switch and applications in fluorescent activated cell sorting
US8771933B2 (en) 2009-10-06 2014-07-08 Massachusetts Institute Of Technology Continuous-flow deformability-based cell separation
EP2311975A1 (en) 2009-10-13 2011-04-20 Iee International Electronics & Engineering S.A. Electrophoretic biomolecules separation device
EP3671180B1 (en) * 2010-01-15 2023-03-08 On-chip Biotechnologies Co., Ltd. Method of using an apparatus configured to separate biological particles
US20110293558A1 (en) 2010-03-22 2011-12-01 Massachusetts Institute Of Technology Material properties of t cells and related methods and compositions
US8705031B2 (en) * 2011-02-04 2014-04-22 Cytonome/St, Llc Particle sorting apparatus and method
AU2012243396A1 (en) 2011-04-15 2013-10-31 The University Of British Columbia Method and apparatus for separation of particles
US10466160B2 (en) * 2011-08-01 2019-11-05 Celsee Diagnostics, Inc. System and method for retrieving and analyzing particles
EP2764347B1 (en) 2011-10-06 2022-04-20 The Regents of The University of California Devices for detecting a particle in a sample and methods for use thereof
US8894946B2 (en) * 2011-10-21 2014-11-25 Integenx Inc. Sample preparation, processing and analysis systems
EP2602608B1 (en) * 2011-12-07 2016-09-14 Imec Analysis and sorting of biological cells in flow
US9297784B2 (en) * 2012-12-05 2016-03-29 Caliper Life Sciences, Inc. Device and method for extracting target objects from a sample
JP2016514965A (ja) 2013-03-15 2016-05-26 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 試料中の細胞を分類する装置及び該装置の使用方法
AU2014235403B2 (en) 2013-03-15 2018-06-28 Beckman Coulter, Inc. Optics system for a flow cytometer
CA2895638A1 (en) * 2013-03-15 2014-09-18 Fluidigm Corporation Methods and devices for analysis of defined multicellular combinations
US10343165B2 (en) * 2013-09-05 2019-07-09 Bio-Rad Laboratories, Inc. On-demand particle dispensing system
US8820538B1 (en) 2014-03-17 2014-09-02 Namocell LLC Method and apparatus for particle sorting
CN110038653A (zh) * 2014-05-27 2019-07-23 伊鲁米那股份有限公司 包括基本仪器和可拆卸盒的用于生物化学分析的系统和方法
EP3154693B1 (en) * 2014-06-11 2021-11-03 PerkinElmer Health Sciences, Inc. Method for performing a sample assay with a microfluidic cartridges with integrated assay controls
KR101702745B1 (ko) * 2014-11-13 2017-02-03 인제대학교 산학협력단 단일세포 분리장치 및 방법
GB2531615B (en) * 2015-02-02 2017-11-22 Atlas Genetics Ltd Instrument for performing a diagnostic test on a fluidic cartridge
JP7130562B2 (ja) 2016-03-17 2022-09-05 ベクトン・ディキンソン・アンド・カンパニー ハイスループット蛍光フローサイトメーターを用いる細胞選別
US10650602B2 (en) 2016-04-15 2020-05-12 Center Of Human-Centered Interaction For Coexistence Apparatus and method for three-dimensional information augmented video see-through display, and rectification apparatus
US10611995B2 (en) 2018-08-15 2020-04-07 Deepcell, Inc. Systems and methods for particle analysis

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003502655A (ja) 1999-06-22 2003-01-21 アジレント・テクノロジーズ・インコーポレーテッド 微小流体デバイスの操作用装置
JP2007533971A (ja) 2003-09-30 2007-11-22 シンギュレックス・インコーポレイテッド 粒子検出の分析精度を改善する方法
JP2006220423A (ja) 2005-02-08 2006-08-24 Japan Science & Technology Agency ゲル電極付セルソーターチップ
WO2016182034A1 (ja) 2015-05-12 2016-11-17 株式会社オンチップ・バイオテクノロジーズ 単一粒子解析方法およびその解析のためのシステム

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