JP7340252B2 - 生物工学的に作製された神経オルガノイド（ｂｅｎｏｓ）の生産方法およびその使用 - Google Patents

生物工学的に作製された神経オルガノイド（ｂｅｎｏｓ）の生産方法およびその使用 Download PDF

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JP7340252B2
JP7340252B2 JP2019567628A JP2019567628A JP7340252B2 JP 7340252 B2 JP7340252 B2 JP 7340252B2 JP 2019567628 A JP2019567628 A JP 2019567628A JP 2019567628 A JP2019567628 A JP 2019567628A JP 7340252 B2 JP7340252 B2 JP 7340252B2
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ヴォルフラム－フーベルトゥス・ツィマーマン
マリア・パタピア・ツァファイリオウ
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ゲオルク－アウグスト－ウニヴェルジテート ゲッティンゲン シュティフトゥング エッフェントリヒェン レッヒツ，ウニヴェルジテーツメディツィン
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JP2019567628A 2017-06-13 2018-06-08 生物工学的に作製された神経オルガノイド（ｂｅｎｏｓ）の生産方法およびその使用 Active JP7340252B2 (ja)

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Application Number Priority Date Filing Date Title
EP17175874 2017-06-13
EP17175874.1 2017-06-13
EP17205951.1 2017-12-07
EP17205951.1A EP3415618B1 (en) 2017-06-13 2017-12-07 Methods of producing bioengineered neuronal organoids (benos) and uses thereof
PCT/EP2018/065204 WO2018228948A1 (en) 2017-06-13 2018-06-08 Methods of producing bioengineered neuronal organoids (benos) and uses thereof

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JP2020523013A JP2020523013A (ja) 2020-08-06
JP2020523013A5 JP2020523013A5 (https=) 2021-08-05
JP7340252B2 true JP7340252B2 (ja) 2023-09-07

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US (2) US11781109B2 (https=)
EP (1) EP3415618B1 (https=)
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KR (1) KR102590084B1 (https=)
CN (1) CN110741078B (https=)
AU (1) AU2018283364B2 (https=)
CA (1) CA3067068C (https=)
DK (1) DK3415618T3 (https=)
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Families Citing this family (13)

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US12599670B2 (en) 2014-12-12 2026-04-14 The Trustees Of The University Of Pennsylvania Composition comprising three-dimensional astrocyte bundles of bi-polar and aligned astrocyte processes and a method of making thereof
WO2017181068A1 (en) 2016-04-14 2017-10-19 The Trustees Of The University Of Pennsylvania Implantable living electrodes and methods for use thereof
CN108456659B (zh) 2018-03-14 2020-05-05 浙江霍德生物工程有限公司 3d大脑类器官的制备方法
WO2020165059A1 (en) * 2019-02-11 2020-08-20 Miltenyi Biotec B.V. & Co. KG Generation of human pluripotent stem cell derived artificial tissue structures without three dimensional matrices
US20220233602A1 (en) * 2019-07-05 2022-07-28 Novo Nordisk A/S Generation of neural stem cell lines derived from human pluripotent stem cells
CN112481212A (zh) * 2020-11-27 2021-03-12 杭州联众医疗科技股份有限公司 一种利用多能干细胞生成脑部类器官的方法
KR102638839B1 (ko) * 2021-04-06 2024-02-23 충북대학교 산학협력단 돼지 신경 오가노이드 제조방법
WO2022256643A1 (en) * 2021-06-04 2022-12-08 The Trustees Of The University Of Pennsylvania Tissue-engineered rostral migratory stream for neuronal replacement
CN115404218B (zh) * 2022-01-13 2024-09-24 南华大学附属第一医院 一种包含胶质细胞的3d人脑类器官培养方法
EP4386384A1 (en) 2022-12-15 2024-06-19 Georg-August-Universität Göttingen Stiftung Öffentlichen Rechts, Universitätsmedizin Potency assay for pharmaceutical products
CN116159182B (zh) * 2023-02-02 2024-09-24 中国科学院苏州纳米技术与纳米仿生研究所 用于治疗脊髓损伤的神经类器官-水凝胶体系及制备方法
CN118272307A (zh) * 2024-04-23 2024-07-02 妙顺（上海）生物科技有限公司 一种神经类器官三维培养诱导分化的方法及其应用
EP4678739A1 (en) * 2024-07-10 2026-01-14 Fundación Pública Andaluza Progreso Y Salud In vitro method for obtaining cortical brain organoids, cortical brain organoids and uses thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016028880A1 (en) 2014-08-19 2016-02-25 Cellular Dynamics International, Inc. Neural networks formed from cells derived from pluripotent stem cells
JP2016506244A (ja) 2012-12-13 2016-03-03 イーエムベーアー−インスティテュート フュール モレクラレ バイオテクロノジー ゲゼルシャフト ミットベシュレンクテル ハフツング 三次元不均一分化組織培養

Family Cites Families (8)

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US8343923B2 (en) * 2007-11-09 2013-01-01 Washington University Use of notch signaling regulators for modulating osteogenesis
US10227563B2 (en) * 2008-06-06 2019-03-12 Riken Method for culture of stem cell
JP6185907B2 (ja) * 2011-03-30 2017-08-23 セルラー ダイナミクス インターナショナル， インコーポレイテッド 神経分化のための多能性幹細胞の予備刺激
JP2016521971A (ja) * 2013-04-23 2016-07-28 イェダ リサーチ アンド ディベロップメント カンパニー リミテッド 単離ナイーブ型多能性幹細胞およびそれを発生させる方法関連出願本出願は、米国特許法１１９条第（ｅ）項に基づき、２０１４年１月２９日出願の米国特許仮出願第６１／９３２，９３５号、２０１３年９月１７日出願の米国特許仮出願第６１／８７８，７６９号、および２０１３年４月２３日出願の米国特許仮出願第６１／８１４，９２０号の優先権を主張する。また、本出願は、同時に提出された同出願人による同時係属出願である、ＹａｑｕｂＨＡＮＮＡ、ＮｏａＮＯＶＥＲＳＨＴＥＲＮ、およびＹｏａｃｈＲＡＩＳによる米国特許出願（発明の名称「単離ナイーブ型多能性幹細胞およびそれを発生させる方法（ＩＳＯＬＡＴＥＤＮＡＩＶＥＰＬＵＲＩＰＯＴＥＮＴＳＴＥＭＣＥＬＬＳＡＮＤＭＥＴＨＯＤＳＯＦＧＥＮＥＲＡＴＩＮＧＳＡＭＥ）」）（代理人事件記録簿第５８８７０号）にも関する。上記出願の内容はその全体を参考として本明細書に組み込む。
AU2015261380B2 (en) * 2014-05-16 2021-04-15 Koninklijke Nederlandse Akademie Van Wetenschappen Improved culture method for organoids
LU92845B1 (en) * 2015-10-08 2017-05-02 Univ Du Luxembourg Campus Belval Means and methods for generating midbrain organoids
WO2017117547A1 (en) * 2015-12-31 2017-07-06 President And Fellows Of Harvard College Methods for generating neural tissue and uses thereof
EP3190176A1 (en) * 2016-01-11 2017-07-12 IMBA-Institut für Molekulare Biotechnologie GmbH Method for tissue culture development on scaffold and differentiated tissue culture

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016506244A (ja) 2012-12-13 2016-03-03 イーエムベーアー−インスティテュート フュール モレクラレ バイオテクロノジー ゲゼルシャフト ミットベシュレンクテル ハフツング 三次元不均一分化組織培養
WO2016028880A1 (en) 2014-08-19 2016-02-25 Cellular Dynamics International, Inc. Neural networks formed from cells derived from pluripotent stem cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Nat Methods，2015年，Vol.12, No.7，pp.671-678
Nature Biotechnology，2017年，Vol.35, No.7，pp.659-666，published online 31 May 2017

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