JP7319027B2 - 培養培地 - Google Patents

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JP7319027B2
JP7319027B2 JP2017528087A JP2017528087A JP7319027B2 JP 7319027 B2 JP7319027 B2 JP 7319027B2 JP 2017528087 A JP2017528087 A JP 2017528087A JP 2017528087 A JP2017528087 A JP 2017528087A JP 7319027 B2 JP7319027 B2 JP 7319027B2
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culture medium
organoids
cells
lung
cell
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JP2018503360A (ja
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ラース ノーマン ザックス
ヤルノ ドロスト
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コーニンクレッカ ネザーランド アカデミー ヴァン ウェテンシャッペン
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    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0695—Stem cells; Progenitor cells; Precursor cells
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/13—Tumour cells, irrespective of tissue of origin
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    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
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    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
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    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
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JP2017528087A 2014-11-27 2015-11-27 培養培地 Active JP7319027B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2021187540A JP2022028828A (ja) 2014-11-27 2021-11-18 培養培地
JP2023197836A JP2024016281A (ja) 2014-11-27 2023-11-22 培養培地

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB1421092.6 2014-11-27
GBGB1421092.6A GB201421092D0 (en) 2014-11-27 2014-11-27 Culture medium
GB1507834.8 2015-05-07
GB1507834.8A GB2532814A (en) 2014-11-27 2015-05-07 Culture medium
PCT/EP2015/077990 WO2016083613A2 (en) 2014-11-27 2015-11-27 Culture medium

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JP2021187540A Division JP2022028828A (ja) 2014-11-27 2021-11-18 培養培地

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JP2018503360A JP2018503360A (ja) 2018-02-08
JP7319027B2 true JP7319027B2 (ja) 2023-08-01

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JP2021187540A Pending JP2022028828A (ja) 2014-11-27 2021-11-18 培養培地
JP2023197836A Pending JP2024016281A (ja) 2014-11-27 2023-11-22 培養培地

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JP2023197836A Pending JP2024016281A (ja) 2014-11-27 2023-11-22 培養培地

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US (2) US11130943B2 (US20070167479A1-20070719-C00034.png)
EP (1) EP3224346A2 (US20070167479A1-20070719-C00034.png)
JP (3) JP7319027B2 (US20070167479A1-20070719-C00034.png)
AU (2) AU2015352351B2 (US20070167479A1-20070719-C00034.png)
CA (1) CA2968736A1 (US20070167479A1-20070719-C00034.png)
GB (2) GB201421092D0 (US20070167479A1-20070719-C00034.png)
HK (1) HK1245323A1 (US20070167479A1-20070719-C00034.png)
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Publication number Priority date Publication date Assignee Title
EP2393917B1 (en) 2009-02-03 2016-04-06 Koninklijke Nederlandse Akademie van Wetenschappen Culture medium for epithelial stem cells and organoids comprising said stem cells.
EP2412800A1 (en) 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Liver organoid, uses thereof and culture method for obtaining them
WO2011140441A2 (en) 2010-05-06 2011-11-10 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
PT3045912T (pt) 2011-12-19 2018-12-12 Koninklijke Nederlandse Akademie Van Wetenschappen Um ensaio quantitativo rápido para medir a função do cftr num modelo de cultura primária intestinal
US10597633B2 (en) 2014-05-16 2020-03-24 Koninklijke Nederlandse Akademie Van Wetenschappen Culture method for organoids
EP3712254A1 (en) 2014-05-28 2020-09-23 Children's Hospital Medical Center Methods and systems for converting precursor cells into gastric tissues through directed differentiation
US11584916B2 (en) 2014-10-17 2023-02-21 Children's Hospital Medical Center Method of making in vivo human small intestine organoids from pluripotent stem cells
GB201421092D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
GB201421094D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
GB201603569D0 (en) 2016-03-01 2016-04-13 Koninklijke Nederlandse Akademie Van Wetenschappen Improved differentiation method
EP4177335A1 (en) 2016-05-05 2023-05-10 Children's Hospital Medical Center Methods for the in vitro manufacture of gastric fundus tissue and compositions related to same
GB201611982D0 (en) 2016-07-11 2016-08-24 Cellesce Ltd Cell culture
US20190161737A1 (en) * 2016-07-27 2019-05-30 Georgetown University Process for continuous cell culture of cancer cells and cancer stem cells
IT201600099380A1 (it) * 2016-10-04 2018-04-04 Univ Bologna Alma Mater Studiorum Composizioni, dispositivi e metodi per il controllo in vitro del microambiente chimico in colture cellulari
WO2018067925A1 (en) * 2016-10-06 2018-04-12 Transgenex Nanobiotech, Inc. Methods for cancer stem cell (csc) expansion
SG10202105768WA (en) 2016-12-05 2021-06-29 Childrens Hospital Med Ct Colonic organoids and methods of making and using same
EP3645704A1 (en) * 2017-06-28 2020-05-06 Rutgers, The State University of New Jersey Single lung cell-derived organoids
WO2019006136A1 (en) * 2017-06-28 2019-01-03 Rutgers, The State University Of New Jersey ORGANOIDS DERIVED FROM A SINGLE VESIC CELL
KR102152492B1 (ko) * 2017-08-14 2020-09-04 울산대학교 산학협력단 3차원 폐암 오가노이드 배양 방법 및 이를 이용한 환자 유래 이종이식 동물모델의 제조 방법
JP7315923B2 (ja) * 2017-09-29 2023-07-27 史敏 石野 臓器オルガノイド及びその製造方法
EP3775162A4 (en) * 2018-06-02 2022-01-05 The University of Hong Kong MATURE AIRWAY ORGANOIDS, THEIR MANUFACTURING PROCESSES AND THEIR USES
GB201903573D0 (en) 2019-03-15 2019-05-01 Koninklijke Nederlandse Akademie Van Wetenschappen Culture method for head and neck organoids
GB201906978D0 (en) 2019-05-17 2019-07-03 Koninklijke Nederlandse Akademie Van Wetenschappen Improved culture method using integrin agonist
WO2020242256A2 (ko) * 2019-05-29 2020-12-03 연세대학교 산학협력단 인간 미뢰 오가노이드의 배양용 조성물
AU2019460208A1 (en) * 2019-08-05 2022-03-17 Genex Health Co., Ltd Method for culturing primary cells of lung cancer solid tumor and primary tumor cells of lung cancer pleural effusion, and supporting reagent
KR102292457B1 (ko) * 2019-10-11 2021-08-23 오가노이드사이언스 주식회사 편도 오가노이드의 제조방법 및 이의 용도
CN110734894B (zh) * 2019-10-11 2021-09-24 纳肽得(青岛)生物医药有限公司 普适性癌症类器官体外培养培养基
US20220411747A1 (en) * 2019-10-31 2022-12-29 University Health Network Medium and methods for culturing organoids
CN112779209B (zh) * 2019-11-08 2023-01-24 合肥中科普瑞昇生物医药科技有限公司 原代乳腺上皮细胞培养基、培养方法及其应用
US11629385B2 (en) 2019-11-22 2023-04-18 Tempus Labs, Inc. Tumor organoid culture compositions, systems, and methods
AU2020392124A1 (en) * 2019-11-25 2022-06-16 The Penn State Research Foundation Composition and method for converting human glial cells into neurons
WO2021113821A1 (en) 2019-12-05 2021-06-10 Tempus Labs, Inc. Systems and methods for high throughput drug screening
CN111424015A (zh) * 2019-12-10 2020-07-17 重庆康克唯生物科技有限公司 用于前列腺肿瘤细胞的培养基和三维培养方法
WO2021168199A1 (en) * 2020-02-19 2021-08-26 Trustees Of Boston University Generation of airway basal stem cells from human pluripotent stem cells
EP3884957A1 (en) * 2020-03-26 2021-09-29 The University of British Columbia Methods for treatment of virus and methods for screening of anti-virus reagent using organoids
US11561178B2 (en) 2020-04-20 2023-01-24 Tempus Labs, Inc. Artificial fluorescent image systems and methods
JPWO2021241493A1 (US20070167479A1-20070719-C00034.png) * 2020-05-25 2021-12-02
US20230203450A1 (en) 2020-05-27 2023-06-29 Otsuka Pharmaceutical Co., Ltd. Method of producing organoid derived from lung epithelial cell or lung cancer cell
CN113969262B (zh) * 2020-07-22 2024-03-26 合肥中科普瑞昇生物医药科技有限公司 一种用于肺癌上皮细胞的培养基、培养方法及其应用
CN112592896B (zh) * 2020-11-22 2024-06-25 深圳市第二人民医院(深圳市转化医学研究院) 一种肺腺癌类器官的培养液及其培养方法
CN113186163A (zh) * 2021-01-18 2021-07-30 南昌五元生物科技有限公司 一种基于p53突变筛选肿瘤类器官的培养方法
KR102652944B1 (ko) * 2021-04-14 2024-03-29 연세대학교 산학협력단 섬유화 튜머로이드의 구조를 유지하는 방법
WO2023278525A1 (en) * 2021-06-29 2023-01-05 Biomarin Pharmaceutical Inc. Cell culture feed for recombinant adeno-associated virus production in mammalian cells
GB202109913D0 (en) 2021-07-09 2021-08-25 Stichting Hubrecht Organoid Tech Organoid-derived monolayers and uses thereof
CN113583962B (zh) * 2021-07-30 2023-03-24 新乡医学院 一种诱导培养基及诱导获得癌细胞持续感染ndv的方法
EP4392547A1 (en) * 2021-08-27 2024-07-03 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt GmbH In vitro method of invasive and ductal cell growth
WO2023099526A1 (en) 2021-11-30 2023-06-08 Université Libre de Bruxelles Generation of thyroid and lung organoids from stem cells
KR20230089159A (ko) * 2021-12-13 2023-06-20 연세대학교 산학협력단 인간 타액선 조직으로부터 분리된 기저 세포 또는 내강 세포 유래 오가노이드의 최적 배양 방법
GB202204266D0 (en) 2022-03-25 2022-05-11 Stichting Hubrecht Organoid Tech Methods for co-culturing genotoxic bacteria and organoids
KR102573391B1 (ko) * 2022-10-05 2023-09-01 오가노이드사이언스 주식회사 침샘 오가노이드 제조용 배지 조성물
KR102627833B1 (ko) * 2023-07-18 2024-01-23 엠비디 주식회사 폐암 유래 세포의 3d 배양용 배지 조성물
CN117887688B (zh) * 2024-03-07 2024-06-25 合肥工业大学 一种高活力、高稳定性的脂肪酶突变体及其编码基因和应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005120547A1 (en) 2004-06-11 2005-12-22 The Institute Of Cancer Research Use of neuregulin-3 for inducing mammary cell differentiation and for the treatment of breast cancer
WO2010119819A1 (ja) 2009-04-17 2010-10-21 国立大学法人東北大学 ヒト肺組織幹細胞の調製方法及びヒト肺胞上皮細胞への分化誘導方法
JP2012525148A (ja) 2009-04-27 2012-10-22 バイアサイト インク 低分子サポート多能性細胞成育およびその方法
JP2014516562A (ja) 2011-06-10 2014-07-17 コーニンクレッカ ネザーランド アカデミー ヴァン ウェテンシャッペン 幹細胞のための培養培地

Family Cites Families (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE208494T1 (de) 1994-05-04 2001-11-15 Mount Sinai Hospital Corp Modulatoren der cytokine der tgf-beta überfamilie und verfahren zu ihrer bestimmung
EP0953633A1 (en) 1998-04-28 1999-11-03 Livercell L.L.C. Cell culturing method and medium for producing proliferated, normal, differentiated human liver cells
AU3859800A (en) 1999-02-24 2000-09-14 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Artificial salivary gland
US20030003088A1 (en) 2001-05-03 2003-01-02 Board Of Trustees Operating Michigan State University Human pancreatic pluripotential stem cell line
WO2003076564A2 (en) 2001-05-16 2003-09-18 The General Hospital Corporation Tissue-engineered organs
US7217570B2 (en) 2001-08-23 2007-05-15 The Wistar Institute Of Anatomy And Biology Organotypic intestinal culture and methods of use thereof
US20050053588A1 (en) 2001-10-18 2005-03-10 Li Yin Conversion of liver stem and progenitor cells to pancreatic functional cells
EP1567639A4 (en) 2002-12-05 2005-12-21 Technion Res & Dev Foundation CULTURE OF HUMAN PANCREATIC ISLANDS AND USES THEREOF
WO2004087896A2 (en) 2003-03-31 2004-10-14 Pfizer Products Inc. Hepatocyte differentiation of stem cells
US20040229355A1 (en) 2003-05-14 2004-11-18 Board Of Regents University Of Texas System Culture medium for long-term culture of hepatocytes
US20070010008A1 (en) 2005-06-29 2007-01-11 Tissuetech, Inc. Ex vivo expansion of primary animal cells
WO2005040391A1 (en) * 2003-10-27 2005-05-06 Murdoch Childrens Research Institute Compositions and methods for differentiating stem cells
US20070036769A9 (en) 2004-06-03 2007-02-15 Linheng Li BMP pathway methods and compositions
US8492148B2 (en) 2005-02-23 2013-07-23 Foundation For Biomedical Research & Innovation Method for amplification of endothelial progenitor cell in vitro
AU2006202209B2 (en) 2005-05-27 2011-04-14 Lifescan, Inc. Amniotic fluid derived cells
CA2621161A1 (en) 2005-09-08 2007-03-15 The Government Of The United States Of America As Represented By The Sec Retary Of The Department Of Health And Human Services Methods for promoting stem cell proliferation and survival
CA2643478C (en) 2006-02-23 2019-06-18 Novocell, Inc. Compositions and methods useful for culturing differentiable cells
US7989204B2 (en) 2006-04-28 2011-08-02 Viacyte, Inc. Hepatocyte lineage cells
US8628964B2 (en) 2006-10-11 2014-01-14 Drexel University Fetal pulmonary cells and uses thereof
ES2564794T5 (es) 2006-10-20 2019-03-14 Deutsches Krebsforschungszentrum Stiftung Des Oeffentlichen Rechts R-espondinas como moduladores de angiogénesis y vasculogénesis
US9251637B2 (en) 2006-11-15 2016-02-02 Bank Of America Corporation Method and apparatus for using at least a portion of a one-time password as a dynamic card verification value
US20080182328A1 (en) 2006-12-19 2008-07-31 The Burnham Institute Mammalian extraembryonic endoderm cells and methods of isolation
WO2008101215A1 (en) 2007-02-16 2008-08-21 Varian Medical Systems Technologies, Inc. Preparative regimen for engraftment, growth and differentiation of non-hematopoeitic cells in vivo
EP2022848A1 (en) 2007-08-10 2009-02-11 Hubrecht Institut A method for identifying, expanding, and removing adult stem cells and cancer stem cells
EP2183353B1 (en) 2007-08-22 2015-04-29 ProBioGen AG Culture system and method for immunogenicity and immunofunction testing in vitro
EP2233565B1 (en) * 2007-12-28 2015-05-13 Fujirebio Inc. Medium for mammalian somatic cells and additive therefor
JP2009195142A (ja) 2008-02-20 2009-09-03 Institute Of Physical & Chemical Research 肝臓卵形細胞の取得方法
CN102144163A (zh) * 2008-04-10 2011-08-03 麻省理工学院 关于鉴定和使用靶向癌症干细胞的试剂的方法
EP2138571B1 (en) 2008-06-26 2017-04-12 SpheroTec GmbH Process for the preparation of multicellular spheroids
WO2010011352A2 (en) 2008-07-25 2010-01-28 The University Of Georgia Research Foundation, Inc. Compositions for mesoderm derived isl1+ multipotent cells (imps), epicardial progenitor cells (epcs) and multipotent cxcr4+cd56+ cells (c56cs) and methods of use
WO2010016766A2 (en) 2008-08-08 2010-02-11 Koninklijke Nederlandse Akademie Van Wetenschappen Antibodies recognizing endogenous human lgr5 and/or lgr6
US9464275B2 (en) 2008-08-21 2016-10-11 The Board Of Trustees Of The Leland Stanford Junior University Ex vivo culture, proliferation and expansion of intestinal epithelium
US20100100396A1 (en) 2008-10-21 2010-04-22 Monitorx, Llc Medical decision-making tool
EP2412800A1 (en) 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Liver organoid, uses thereof and culture method for obtaining them
US9752124B2 (en) * 2009-02-03 2017-09-05 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for epithelial stem cells and organoids comprising the stem cells
EP2393917B1 (en) 2009-02-03 2016-04-06 Koninklijke Nederlandse Akademie van Wetenschappen Culture medium for epithelial stem cells and organoids comprising said stem cells.
US20120207744A1 (en) 2009-03-19 2012-08-16 Mendlein John D Reprogramming compositions and methods of using the same
WO2016094457A1 (en) 2014-12-08 2016-06-16 Ray Partha S Methods for treating basal-like and claudin-low breast cancer and combination therapies thereof
CN102741695B (zh) 2010-02-11 2015-08-26 霍夫曼-拉罗奇有限公司 3d adcc nk facs测定法
WO2011159726A2 (en) 2010-06-14 2011-12-22 The Scripps Research Institute Reprogramming of cells to a new fate
GB201014169D0 (en) 2010-08-25 2010-10-06 Cambridge Entpr Ltd In vitro hepatic differentiation
SG188666A1 (en) 2010-09-30 2013-05-31 Agency Science Tech & Res Methods and reagents for detection and treatment of esophageal metaplasia
WO2012087965A2 (en) 2010-12-22 2012-06-28 Fate Therapauetics, Inc. Cell culture platform for single cell sorting and enhanced reprogramming of ipscs
GB201106395D0 (en) 2011-04-14 2011-06-01 Hubrecht Inst Compounds
WO2013003729A1 (en) * 2011-06-29 2013-01-03 The Penn State Research Foundation Compositions, methods and kits for treating leukemia
US20130052729A1 (en) 2011-08-29 2013-02-28 Olivier Pourquie Method for preparing induced paraxial mesoderm progenitor (ipam) cells and their use
WO2013052155A1 (en) 2011-10-05 2013-04-11 Genentech, Inc. Methods of treating liver conditions using notch2 antagonists
GB201117469D0 (en) * 2011-10-10 2011-11-23 Cell Guidance Systems Ltd Culture media for pluripotent stem cells
WO2013061608A1 (ja) 2011-10-27 2013-05-02 国立大学法人東京医科歯科大学 大腸上皮幹細胞の単離・培養技術と、これを用いた大腸上皮移植技術
PT3045912T (pt) 2011-12-19 2018-12-12 Koninklijke Nederlandse Akademie Van Wetenschappen Um ensaio quantitativo rápido para medir a função do cftr num modelo de cultura primária intestinal
WO2013131000A1 (en) * 2012-03-01 2013-09-06 Whitehead Institute For Biomedical Research Emt-inducing transcription factors cooperate with sox9
CA2886396C (en) 2012-07-23 2023-08-22 Institute Of Biophysics, Chinese Academy Of Sciences Method for inducing pluripotent stem cells to differentiate into ventricular cardiomyocytes in vitro
WO2014066649A1 (en) * 2012-10-26 2014-05-01 The Regents Of The University Of California A strategy for engineering various 3d tissues, organoids and vasculature
WO2014127170A1 (en) 2013-02-13 2014-08-21 Wake Forest University Health Sciences Bioengineered liver constructs and methods relating thereto
US20150368616A1 (en) 2013-02-14 2015-12-24 The Cleveland Clinic Foundation Methods for induction of cell fates from pluripotent cells
WO2014124527A1 (en) 2013-02-18 2014-08-21 University Health Network Methods for generating hepatocytes and cholangiocytes from pluripotent stem cells
SI2970890T1 (sl) 2013-03-14 2020-10-30 The Brigham And Women's Hospital, Inc. Sestavki in postopki za razmnoževanje in kultiviranje epitelijskih matičnih celic
KR102588702B1 (ko) * 2013-03-15 2023-10-16 더 보드 오브 리젠츠 오브 더 유니버시티 오브 텍사스 시스템 누트린-3a 및 펩티드에 의한 폐 섬유증의 저해
US10251934B2 (en) * 2013-04-16 2019-04-09 Orbsen Therapeutics Limited Syndecan-2 compositions and methods of use
US10597633B2 (en) 2014-05-16 2020-03-24 Koninklijke Nederlandse Akademie Van Wetenschappen Culture method for organoids
AU2015264315B2 (en) 2014-05-19 2018-08-02 Transgenex Nanobiotech, Inc. Formation of multicellular tumoroids and uses thereof
US11920164B2 (en) 2014-07-30 2024-03-05 Yeda Research And Development Co. Ltd. Media for culturing naive human pluripotent stem cells
SG11201701844YA (en) 2014-10-08 2017-04-27 Agency Science Tech & Res Methods of differentiating stem cells into liver cell lineages
GB201421092D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
GB201421094D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
US20180187191A1 (en) 2015-06-15 2018-07-05 Texas Tech University System Targeted-Delivery of Small Interference RNA Against Anthrax
US20180258400A1 (en) 2015-09-15 2018-09-13 Agency For Science, Technology And Research (A*Star) Derivation of liver organoids from human pluripotent stem cells
CN106754636B (zh) 2015-11-19 2019-08-30 中国人民解放军第二军医大学 体外诱导原代肝细胞胆管化并长期培养、扩增和分化的方法及其应用
GB201603569D0 (en) 2016-03-01 2016-04-13 Koninklijke Nederlandse Akademie Van Wetenschappen Improved differentiation method
WO2017205511A1 (en) 2016-05-25 2017-11-30 Salk Institute For Biological Studies Compositions and methods for organoid generation and disease modeling
GB201610748D0 (en) 2016-06-20 2016-08-03 Koninklijke Nederlandse Akademie Van Wetenschappen Improved diffrentation method
GB201721615D0 (en) 2017-12-21 2018-02-07 Koninklijke Nederlandse Akademie Van Wetenschappen Immune cell organoid co-cultures

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005120547A1 (en) 2004-06-11 2005-12-22 The Institute Of Cancer Research Use of neuregulin-3 for inducing mammary cell differentiation and for the treatment of breast cancer
WO2010119819A1 (ja) 2009-04-17 2010-10-21 国立大学法人東北大学 ヒト肺組織幹細胞の調製方法及びヒト肺胞上皮細胞への分化誘導方法
JP2012525148A (ja) 2009-04-27 2012-10-22 バイアサイト インク 低分子サポート多能性細胞成育およびその方法
JP2014516562A (ja) 2011-06-10 2014-07-17 コーニンクレッカ ネザーランド アカデミー ヴァン ウェテンシャッペン 幹細胞のための培養培地

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ＩＮ ＶＩＴＲＯ，１９８０年，Ｖｏｌ．１６，Ｎｏ．１１，ｐ．９９４－１００６
Ｌｅｊｌａ Ｐａｓｉｃ ｅｔ ａｌ．，Ｓｕｓｔａｉｎｅｄ ａｃｔｉｖａｔｉｏｎ ｏｆ ｔｈｅ ＨＥＲ１－ＥＲＫ１／２－ＲＳＫ ｓｉｇｎａｌｉｎｇ ｐａｔｈｗａｙ ｃｏｎｔｒｏｌｓ ｍｙｏｅｐｉｔｈｅｌｉａｌ ｃｅｌｌ ｆａｔｅ ｉｎ ｈｕｍａｎ ｍａｍｍａｒｙ ｔｉｓｓｕｅ，ＧＥＮＥＳ ＆ ＤＥＶＥＬＯＰＭＥＮＴ，２０１１年，Ｖｏｌ．２５， ｐ．１６４１－１６５３
Ｍｏｌｅｃｕｌａｒ ａｎｄ Ｃｅｌｌｕｌａｒ Ｂｉｏｃｈｅｍｉｓｔｒｙ，２００７年，Ｖｏｌ．３０５，ｐ．１７１－１７８

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