JP7308468B2 - 凝集の少ないｐｐｒタンパク質及びその利用 - Google Patents

凝集の少ないｐｐｒタンパク質及びその利用 Download PDF

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Publication number
JP7308468B2
JP7308468B2 JP2021521908A JP2021521908A JP7308468B2 JP 7308468 B2 JP7308468 B2 JP 7308468B2 JP 2021521908 A JP2021521908 A JP 2021521908A JP 2021521908 A JP2021521908 A JP 2021521908A JP 7308468 B2 JP7308468 B2 JP 7308468B2
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amino acids
sequence
amino acid
ppr
formula
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JPWO2020241877A1 (https=
Inventor
祐介 八木
崇喜 今井
喬之 玉井
崇裕 中村
岳大 寺本
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EDITFORCE, INC.
Kyushu University NUC
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EDITFORCE, INC.
Kyushu University NUC
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Publication of JPWO2020241877A1 publication Critical patent/JPWO2020241877A1/ja
Priority to JP2022208226A priority Critical patent/JP7526399B2/ja
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Priority to JP2024109757A priority patent/JP7749763B2/ja
Priority to JP2025158040A priority patent/JP2025181985A/ja
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Environmental Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
JP2021521908A 2019-05-29 2020-05-29 凝集の少ないｐｐｒタンパク質及びその利用 Active JP7308468B2 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2022208226A JP7526399B2 (ja) 2019-05-29 2022-12-26 凝集の少ないｐｐｒタンパク質及びその利用
JP2024109757A JP7749763B2 (ja) 2019-05-29 2024-07-08 凝集の少ないｐｐｒタンパク質及びその利用
JP2025158040A JP2025181985A (ja) 2019-05-29 2025-09-24 凝集の少ないｐｐｒタンパク質及びその利用

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019100553 2019-05-29
JP2019100553 2019-05-29
PCT/JP2020/021473 WO2020241877A1 (ja) 2019-05-29 2020-05-29 凝集の少ないｐｐｒタンパク質及びその利用

Related Child Applications (1)

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JP2022208226A Division JP7526399B2 (ja) 2019-05-29 2022-12-26 凝集の少ないｐｐｒタンパク質及びその利用

Publications (2)

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JPWO2020241877A1 JPWO2020241877A1 (https=) 2020-12-03
JP7308468B2 true JP7308468B2 (ja) 2023-07-14

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JP2021521908A Active JP7308468B2 (ja) 2019-05-29 2020-05-29 凝集の少ないｐｐｒタンパク質及びその利用
JP2022208226A Active JP7526399B2 (ja) 2019-05-29 2022-12-26 凝集の少ないｐｐｒタンパク質及びその利用
JP2024109757A Active JP7749763B2 (ja) 2019-05-29 2024-07-08 凝集の少ないｐｐｒタンパク質及びその利用
JP2025158040A Pending JP2025181985A (ja) 2019-05-29 2025-09-24 凝集の少ないｐｐｒタンパク質及びその利用

Family Applications After (3)

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JP2022208226A Active JP7526399B2 (ja) 2019-05-29 2022-12-26 凝集の少ないｐｐｒタンパク質及びその利用
JP2024109757A Active JP7749763B2 (ja) 2019-05-29 2024-07-08 凝集の少ないｐｐｒタンパク質及びその利用
JP2025158040A Pending JP2025181985A (ja) 2019-05-29 2025-09-24 凝集の少ないｐｐｒタンパク質及びその利用

Country Status (10)

Country Link
US (1) US12559528B2 (https=)
EP (1) EP3977849A4 (https=)
JP (4) JP7308468B2 (https=)
KR (1) KR102898966B1 (https=)
CN (1) CN113966340A (https=)
AU (2) AU2020283367B2 (https=)
CA (1) CA3142303A1 (https=)
IL (2) IL288417B2 (https=)
SG (1) SG11202113057XA (https=)
WO (1) WO2020241877A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7125727B1 (ja) 2021-09-07 2022-08-25 国立大学法人千葉大学 核酸配列改変用組成物および核酸配列の標的部位を改変する方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006188507A (ja) * 2004-12-10 2006-07-20 Tokyo Univ Of Agriculture & Technology 蛋白質の溶解度向上方法
WO2013058404A1 (ja) 2011-10-21 2013-04-25 国立大学法人九州大学 Pprモチーフを利用したrna結合性蛋白質の設計方法及びその利用
KR102893770B1 (ko) * 2013-04-22 2025-11-28 고쿠리쓰다이가쿠호진 규슈다이가쿠 피피알 단백질을 이용하는 dna 결합성 단백질 및 그의 이용
WO2017209122A1 (ja) 2016-06-03 2017-12-07 国立大学法人九州大学 標的ｍＲＮＡからのタンパク質発現量を向上させるための融合タンパク質
JPWO2018030488A1 (ja) 2016-08-10 2019-06-13 富士フイルム和光純薬株式会社 Ｐｐｒモチーフを利用したｄｎａ結合性タンパク質およびその利用
JP6918686B2 (ja) 2017-11-28 2021-08-11 東芝ライフスタイル株式会社 冷蔵庫
KR102568600B1 (ko) 2018-02-19 2023-08-23 삼성디스플레이 주식회사 표시 장치, 이를 포함하는 적층 기판, 및 표시 장치 제조방법
CN114729017A (zh) * 2019-05-29 2022-07-08 日商基因编辑力股份有限公司 Ppr蛋白的有效制作方法及其应用

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Ｂｉｏｏｒｇ Ｍｅｄ Ｃｈｅｍ Ｌｅｔｔ，（２０１０），Ｖｏｌ．２０，ｐ．１７７６－１７７８
Ｊｏｕｒｎａｌ ｏｆ Ｃｈｒｏｍａｔｏｇｒａｐｈｙ Ｂ，２００９年１１月１５日，Ｖｏｌ．８７７ Ｎｏ．３０，Ｐａｇｅ．３８０６－３８１２
Ｎａｔｕｒｅ ｃｏｍｍｕｎｉｃａｔｉｏｎｓ，２０１４年，ｄｏｉ：１０．１０３８／ｎｃｏｍｍｓ６７２９
Ｎａｔｕｒｅ ｃｏｍｍｕｎｉｃａｔｉｏｎｓ，２０１６年，ｄｏｉ：１０．１０３８／ｎｃｏｍｍｓ１１２８５
八木 祐介，内在配列を標的としたＲＮＡイメージング手法の開発，科学研究費助成事業研究成果報告書，２０１６年１０月２７日

Also Published As

Publication number Publication date
EP3977849A1 (en) 2022-04-06
JP7749763B2 (ja) 2025-10-06
CN113966340A (zh) 2022-01-21
IL312349B1 (en) 2024-12-01
IL288417B2 (en) 2024-10-01
CA3142303A1 (en) 2020-12-03
JP2024138381A (ja) 2024-10-08
IL312349B2 (en) 2025-04-01
IL288417B1 (en) 2024-06-01
AU2020283367B2 (en) 2025-04-10
SG11202113057XA (en) 2021-12-30
US20220220166A1 (en) 2022-07-14
JPWO2020241877A1 (https=) 2020-12-03
BR112021023937A2 (pt) 2022-04-19
WO2020241877A1 (ja) 2020-12-03
IL312349A (en) 2024-06-01
JP7526399B2 (ja) 2024-08-01
AU2025205185A1 (en) 2025-07-31
EP3977849A4 (en) 2023-06-28
KR20220023984A (ko) 2022-03-03
IL288417A (en) 2022-01-01
AU2020283367A1 (en) 2022-01-06
US12559528B2 (en) 2026-02-24
NZ783357A (en) 2025-06-27
JP2023036848A (ja) 2023-03-14
KR102898966B1 (ko) 2025-12-10
JP2025181985A (ja) 2025-12-11

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